Experiment: EMM_noCarbon with Sucrose and Putrescine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF28 and GFF29 are separated by 330 nucleotides GFF29 and GFF30 overlap by 1 nucleotides GFF30 and GFF31 are separated by 299 nucleotides GFF31 and GFF32 are separated by 77 nucleotides GFF32 and GFF33 are separated by 98 nucleotides GFF33 and GFF34 are separated by 62 nucleotides
GFF28 - hypothetical protein, at 25,314 to 25,427
GFF28
GFF29 - hypothetical protein, at 25,758 to 26,288
GFF29
GFF30 - hypothetical protein, at 26,288 to 26,803
GFF30
GFF31 - Phage lysin (EC 3.2.1.17) # Phage lysozyme or muramidase (EC 3.2.1.17), at 27,103 to 27,312
GFF31
GFF32 - hypothetical protein, at 27,390 to 27,506
GFF32
GFF33 - hypothetical protein, at 27,605 to 27,721
GFF33
GFF34 - hypothetical protein, at 27,784 to 27,912
GFF34
Position (kb)
26
27 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 25.371 kb on + strand, within GFF28 at 25.405 kb on + strand, within GFF28 at 25.405 kb on + strand, within GFF28 at 25.415 kb on + strand, within GFF28 at 25.415 kb on + strand, within GFF28 at 25.419 kb on + strand at 25.419 kb on + strand at 25.420 kb on + strand at 25.424 kb on + strand at 25.424 kb on + strand at 25.428 kb on - strand at 25.428 kb on - strand at 25.432 kb on - strand at 25.433 kb on - strand at 25.433 kb on - strand at 25.437 kb on - strand at 25.437 kb on - strand at 25.480 kb on + strand at 25.492 kb on + strand at 25.521 kb on + strand at 25.524 kb on + strand at 25.524 kb on - strand at 25.538 kb on + strand at 25.538 kb on + strand at 25.539 kb on - strand at 25.557 kb on + strand at 25.578 kb on - strand at 25.578 kb on - strand at 25.594 kb on + strand at 25.614 kb on + strand at 25.614 kb on + strand at 25.614 kb on + strand at 25.622 kb on - strand at 25.622 kb on - strand at 25.634 kb on + strand at 25.644 kb on - strand at 25.644 kb on - strand at 25.775 kb on + strand at 25.783 kb on - strand at 25.818 kb on - strand, within GFF29 at 25.831 kb on - strand, within GFF29 at 25.841 kb on + strand, within GFF29 at 25.844 kb on + strand, within GFF29 at 25.849 kb on + strand, within GFF29 at 25.849 kb on + strand, within GFF29 at 25.857 kb on - strand, within GFF29 at 25.857 kb on - strand, within GFF29 at 25.857 kb on - strand, within GFF29 at 25.857 kb on - strand, within GFF29 at 25.857 kb on - strand, within GFF29 at 25.857 kb on - strand, within GFF29 at 25.892 kb on + strand, within GFF29 at 25.892 kb on + strand, within GFF29 at 25.892 kb on + strand, within GFF29 at 25.900 kb on - strand, within GFF29 at 25.934 kb on + strand, within GFF29 at 25.939 kb on + strand, within GFF29 at 25.939 kb on + strand, within GFF29 at 25.939 kb on + strand, within GFF29 at 25.947 kb on - strand, within GFF29 at 25.947 kb on - strand, within GFF29 at 25.947 kb on - strand, within GFF29 at 25.947 kb on - strand, within GFF29 at 25.947 kb on - strand, within GFF29 at 25.947 kb on - strand, within GFF29 at 25.949 kb on + strand, within GFF29 at 25.949 kb on + strand, within GFF29 at 25.949 kb on + strand, within GFF29 at 25.949 kb on + strand, within GFF29 at 25.952 kb on - strand, within GFF29 at 25.957 kb on - strand, within GFF29 at 25.959 kb on + strand, within GFF29 at 25.965 kb on + strand, within GFF29 at 25.981 kb on + strand, within GFF29 at 26.004 kb on - strand, within GFF29 at 26.004 kb on - strand, within GFF29 at 26.006 kb on + strand, within GFF29 at 26.025 kb on + strand, within GFF29 at 26.025 kb on + strand, within GFF29 at 26.033 kb on - strand, within GFF29 at 26.033 kb on - strand, within GFF29 at 26.033 kb on - strand, within GFF29 at 26.073 kb on - strand, within GFF29 at 26.088 kb on + strand, within GFF29 at 26.098 kb on - strand, within GFF29 at 26.103 kb on - strand, within GFF29 at 26.108 kb on + strand, within GFF29 at 26.149 kb on - strand, within GFF29 at 26.172 kb on + strand, within GFF29 at 26.172 kb on + strand, within GFF29 at 26.172 kb on + strand, within GFF29 at 26.172 kb on + strand, within GFF29 at 26.180 kb on - strand, within GFF29 at 26.180 kb on - strand, within GFF29 at 26.180 kb on - strand, within GFF29 at 26.211 kb on + strand, within GFF29 at 26.216 kb on + strand, within GFF29 at 26.219 kb on - strand, within GFF29 at 26.219 kb on - strand, within GFF29 at 26.219 kb on - strand, within GFF29 at 26.246 kb on + strand at 26.254 kb on - strand at 26.254 kb on - strand at 26.263 kb on - strand at 26.278 kb on - strand at 26.278 kb on - strand at 26.278 kb on - strand at 26.278 kb on - strand at 26.467 kb on + strand, within GFF30 at 26.467 kb on + strand, within GFF30 at 26.488 kb on + strand, within GFF30 at 26.502 kb on + strand, within GFF30 at 26.515 kb on + strand, within GFF30 at 26.517 kb on + strand, within GFF30 at 26.535 kb on + strand, within GFF30 at 26.535 kb on + strand, within GFF30 at 26.538 kb on - strand, within GFF30 at 26.538 kb on - strand, within GFF30 at 26.538 kb on - strand, within GFF30 at 26.543 kb on - strand, within GFF30 at 26.543 kb on - strand, within GFF30 at 26.553 kb on - strand, within GFF30 at 26.577 kb on + strand, within GFF30 at 26.580 kb on - strand, within GFF30 at 26.592 kb on + strand, within GFF30 at 26.592 kb on + strand, within GFF30 at 26.593 kb on - strand, within GFF30 at 26.642 kb on + strand, within GFF30 at 26.650 kb on - strand, within GFF30 at 26.650 kb on - strand, within GFF30 at 26.650 kb on - strand, within GFF30 at 26.655 kb on + strand, within GFF30 at 26.685 kb on - strand, within GFF30 at 26.952 kb on + strand at 26.981 kb on + strand at 26.987 kb on + strand at 26.994 kb on - strand at 27.004 kb on - strand at 27.026 kb on + strand at 27.026 kb on - strand at 27.111 kb on + strand at 27.127 kb on + strand, within GFF31 at 27.135 kb on - strand, within GFF31 at 27.137 kb on - strand, within GFF31 at 27.197 kb on + strand, within GFF31 at 27.200 kb on - strand, within GFF31 at 27.205 kb on + strand, within GFF31 at 27.256 kb on + strand, within GFF31 at 27.256 kb on + strand, within GFF31 at 27.264 kb on - strand, within GFF31 at 27.278 kb on - strand, within GFF31 at 27.309 kb on - strand at 27.340 kb on - strand at 27.369 kb on - strand at 27.388 kb on + strand at 27.396 kb on - strand at 27.396 kb on - strand at 27.396 kb on - strand at 27.407 kb on + strand, within GFF32 at 27.410 kb on - strand, within GFF32 at 27.410 kb on - strand, within GFF32 at 27.410 kb on - strand, within GFF32 at 27.410 kb on - strand, within GFF32 at 27.441 kb on + strand, within GFF32 at 27.556 kb on + strand at 27.591 kb on + strand at 27.591 kb on + strand at 27.591 kb on + strand at 27.591 kb on + strand at 27.591 kb on + strand at 27.591 kb on + strand at 27.599 kb on - strand at 27.599 kb on - strand at 27.606 kb on + strand at 27.650 kb on - strand, within GFF33 at 27.700 kb on - strand, within GFF33 at 27.713 kb on - strand at 27.729 kb on + strand at 27.731 kb on - strand at 27.731 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction EMM_noCarbon with Sucrose and Putrescine remove 25,371 + GFF28 0.50 +1.9 25,405 + GFF28 0.80 +1.4 25,405 + GFF28 0.80 -0.0 25,415 + GFF28 0.89 -1.6 25,415 + GFF28 0.89 +0.2 25,419 + +0.9 25,419 + -0.3 25,420 + +0.3 25,424 + +0.2 25,424 + -0.8 25,428 - -0.3 25,428 - +2.0 25,432 - +0.0 25,433 - +0.4 25,433 - +0.3 25,437 - -1.3 25,437 - -0.0 25,480 + +0.2 25,492 + -1.6 25,521 + -1.7 25,524 + -0.5 25,524 - -0.4 25,538 + +0.5 25,538 + +0.5 25,539 - +0.1 25,557 + +0.8 25,578 - -2.9 25,578 - +1.3 25,594 + +0.3 25,614 + +0.3 25,614 + -1.5 25,614 + +1.1 25,622 - +0.1 25,622 - -0.2 25,634 + +0.4 25,644 - -0.4 25,644 - -0.2 25,775 + -1.0 25,783 - +0.2 25,818 - GFF29 0.11 +1.3 25,831 - GFF29 0.14 -0.4 25,841 + GFF29 0.16 +1.7 25,844 + GFF29 0.16 -1.1 25,849 + GFF29 0.17 +0.6 25,849 + GFF29 0.17 +0.6 25,857 - GFF29 0.19 -0.9 25,857 - GFF29 0.19 +0.3 25,857 - GFF29 0.19 +1.1 25,857 - GFF29 0.19 -0.1 25,857 - GFF29 0.19 -0.2 25,857 - GFF29 0.19 +0.3 25,892 + GFF29 0.25 +1.1 25,892 + GFF29 0.25 -0.3 25,892 + GFF29 0.25 -1.7 25,900 - GFF29 0.27 -0.6 25,934 + GFF29 0.33 -0.9 25,939 + GFF29 0.34 -1.9 25,939 + GFF29 0.34 +0.6 25,939 + GFF29 0.34 +1.5 25,947 - GFF29 0.36 +0.8 25,947 - GFF29 0.36 +0.6 25,947 - GFF29 0.36 +1.0 25,947 - GFF29 0.36 +0.4 25,947 - GFF29 0.36 -0.2 25,947 - GFF29 0.36 +0.2 25,949 + GFF29 0.36 +0.5 25,949 + GFF29 0.36 -0.6 25,949 + GFF29 0.36 -2.0 25,949 + GFF29 0.36 +0.2 25,952 - GFF29 0.37 +0.7 25,957 - GFF29 0.37 +0.5 25,959 + GFF29 0.38 -0.4 25,965 + GFF29 0.39 -0.2 25,981 + GFF29 0.42 -0.0 26,004 - GFF29 0.46 -0.0 26,004 - GFF29 0.46 +0.3 26,006 + GFF29 0.47 -0.3 26,025 + GFF29 0.50 -0.4 26,025 + GFF29 0.50 -0.8 26,033 - GFF29 0.52 -0.5 26,033 - GFF29 0.52 +1.0 26,033 - GFF29 0.52 +0.7 26,073 - GFF29 0.59 -0.6 26,088 + GFF29 0.62 -1.0 26,098 - GFF29 0.64 -0.5 26,103 - GFF29 0.65 +0.9 26,108 + GFF29 0.66 -0.6 26,149 - GFF29 0.74 +1.1 26,172 + GFF29 0.78 +2.5 26,172 + GFF29 0.78 -2.0 26,172 + GFF29 0.78 -1.7 26,172 + GFF29 0.78 -1.5 26,180 - GFF29 0.79 -0.8 26,180 - GFF29 0.79 +1.5 26,180 - GFF29 0.79 +1.4 26,211 + GFF29 0.85 +0.1 26,216 + GFF29 0.86 +1.5 26,219 - GFF29 0.87 +0.2 26,219 - GFF29 0.87 -0.3 26,219 - GFF29 0.87 -0.2 26,246 + +0.7 26,254 - -0.2 26,254 - +0.5 26,263 - +0.1 26,278 - +0.1 26,278 - +0.1 26,278 - -0.5 26,278 - +0.6 26,467 + GFF30 0.35 +0.6 26,467 + GFF30 0.35 +1.0 26,488 + GFF30 0.39 -1.2 26,502 + GFF30 0.41 +1.5 26,515 + GFF30 0.44 +0.9 26,517 + GFF30 0.44 -0.4 26,535 + GFF30 0.48 +0.0 26,535 + GFF30 0.48 +0.1 26,538 - GFF30 0.48 +0.4 26,538 - GFF30 0.48 +0.7 26,538 - GFF30 0.48 +1.2 26,543 - GFF30 0.49 +1.1 26,543 - GFF30 0.49 -0.0 26,553 - GFF30 0.51 -0.2 26,577 + GFF30 0.56 +0.1 26,580 - GFF30 0.57 +0.9 26,592 + GFF30 0.59 -1.5 26,592 + GFF30 0.59 +0.3 26,593 - GFF30 0.59 +0.9 26,642 + GFF30 0.69 -1.2 26,650 - GFF30 0.70 -0.3 26,650 - GFF30 0.70 +0.8 26,650 - GFF30 0.70 +0.8 26,655 + GFF30 0.71 -0.2 26,685 - GFF30 0.77 -0.6 26,952 + -1.1 26,981 + -1.2 26,987 + +1.1 26,994 - +0.4 27,004 - +0.2 27,026 + +0.9 27,026 - -0.1 27,111 + -0.3 27,127 + GFF31 0.11 -0.7 27,135 - GFF31 0.15 -0.0 27,137 - GFF31 0.16 -0.4 27,197 + GFF31 0.45 +0.3 27,200 - GFF31 0.46 +0.7 27,205 + GFF31 0.49 -0.0 27,256 + GFF31 0.73 +0.1 27,256 + GFF31 0.73 +0.1 27,264 - GFF31 0.77 +0.1 27,278 - GFF31 0.83 +0.1 27,309 - +0.2 27,340 - +1.5 27,369 - +0.6 27,388 + -0.7 27,396 - -0.4 27,396 - -1.6 27,396 - -0.1 27,407 + GFF32 0.15 +0.4 27,410 - GFF32 0.17 +0.2 27,410 - GFF32 0.17 -0.9 27,410 - GFF32 0.17 -1.2 27,410 - GFF32 0.17 +1.3 27,441 + GFF32 0.44 +0.6 27,556 + +0.7 27,591 + -1.6 27,591 + -2.2 27,591 + +0.3 27,591 + +0.1 27,591 + +1.3 27,591 + -1.5 27,599 - -0.2 27,599 - +1.3 27,606 + -1.6 27,650 - GFF33 0.38 -0.6 27,700 - GFF33 0.81 -1.8 27,713 - +1.1 27,729 + -1.1 27,731 - -0.4 27,731 - +0.4
Or see this region's nucleotide sequence