Strain Fitness in Sphingobium sp. HT1-2 around GFF253

Experiment: EMM_noCarbon with Sucrose and Putrescine

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF251 and GFF252 are separated by 74 nucleotidesGFF252 and GFF253 are separated by 6 nucleotidesGFF253 and GFF254 are separated by 98 nucleotides GFF251 - Site-specific tyrosine recombinase XerC, at 255,302 to 256,204 GFF251 GFF252 - hypothetical protein, at 256,279 to 256,572 GFF252 GFF253 - DedA, at 256,579 to 256,719 GFF253 GFF254 - hypothetical protein, at 256,818 to 258,431 GFF254 Position (kb) 256 257Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3at 255.597 kb on + strand, within GFF251at 255.618 kb on - strand, within GFF251at 255.635 kb on + strand, within GFF251at 255.648 kb on - strand, within GFF251at 255.673 kb on - strand, within GFF251at 255.707 kb on + strand, within GFF251at 255.745 kb on + strand, within GFF251at 255.770 kb on + strand, within GFF251at 255.810 kb on + strand, within GFF251at 255.823 kb on - strand, within GFF251at 255.833 kb on - strand, within GFF251at 255.852 kb on - strand, within GFF251at 255.862 kb on - strand, within GFF251at 255.892 kb on - strand, within GFF251at 255.902 kb on - strand, within GFF251at 255.961 kb on + strand, within GFF251at 255.984 kb on + strand, within GFF251at 255.984 kb on + strand, within GFF251at 255.984 kb on + strand, within GFF251at 256.030 kb on - strand, within GFF251at 256.035 kb on - strand, within GFF251at 256.040 kb on - strand, within GFF251at 256.053 kb on - strand, within GFF251at 256.064 kb on + strand, within GFF251at 256.093 kb on - strand, within GFF251at 256.097 kb on + strand, within GFF251at 256.103 kb on + strand, within GFF251at 256.106 kb on + strand, within GFF251at 256.114 kb on - strandat 256.124 kb on - strandat 256.128 kb on + strandat 256.133 kb on + strandat 256.138 kb on + strandat 256.187 kb on + strandat 256.195 kb on - strandat 256.206 kb on - strandat 256.217 kb on - strandat 256.229 kb on - strandat 256.239 kb on - strandat 256.239 kb on - strandat 256.241 kb on + strandat 256.241 kb on + strandat 256.249 kb on - strandat 256.249 kb on - strandat 256.256 kb on + strandat 256.272 kb on + strandat 256.277 kb on + strandat 256.280 kb on - strandat 256.290 kb on + strandat 256.297 kb on + strandat 256.298 kb on - strandat 256.298 kb on - strandat 256.315 kb on - strand, within GFF252at 256.358 kb on - strand, within GFF252at 256.359 kb on - strand, within GFF252at 256.423 kb on - strand, within GFF252at 256.449 kb on + strand, within GFF252at 256.463 kb on + strand, within GFF252at 256.473 kb on + strand, within GFF252at 256.473 kb on + strand, within GFF252at 256.482 kb on + strand, within GFF252at 256.482 kb on + strand, within GFF252at 256.482 kb on + strand, within GFF252at 256.482 kb on + strand, within GFF252at 256.482 kb on + strand, within GFF252at 256.483 kb on + strand, within GFF252at 256.483 kb on + strand, within GFF252at 256.490 kb on - strand, within GFF252at 256.490 kb on - strand, within GFF252at 256.490 kb on - strand, within GFF252at 256.490 kb on - strand, within GFF252at 256.491 kb on - strand, within GFF252at 256.507 kb on + strand, within GFF252at 256.509 kb on - strand, within GFF252at 256.519 kb on - strand, within GFF252at 256.532 kb on + strand, within GFF252at 256.532 kb on - strand, within GFF252at 256.539 kb on - strand, within GFF252at 256.539 kb on - strand, within GFF252at 256.539 kb on - strand, within GFF252at 256.540 kb on - strand, within GFF252at 256.540 kb on - strand, within GFF252at 256.541 kb on + strand, within GFF252at 256.549 kb on - strandat 256.549 kb on - strandat 256.576 kb on + strandat 256.576 kb on + strandat 256.584 kb on - strandat 256.584 kb on - strandat 256.584 kb on - strandat 256.584 kb on - strandat 256.584 kb on - strandat 256.584 kb on - strandat 256.608 kb on + strand, within GFF253at 256.616 kb on - strand, within GFF253at 256.616 kb on - strand, within GFF253at 256.618 kb on + strand, within GFF253at 256.618 kb on + strand, within GFF253at 256.618 kb on + strand, within GFF253at 256.618 kb on + strand, within GFF253at 256.618 kb on + strand, within GFF253at 256.621 kb on - strand, within GFF253at 256.621 kb on - strand, within GFF253at 256.621 kb on - strand, within GFF253at 256.621 kb on - strand, within GFF253at 256.623 kb on + strand, within GFF253at 256.623 kb on + strand, within GFF253at 256.626 kb on - strand, within GFF253at 256.626 kb on - strand, within GFF253at 256.626 kb on - strand, within GFF253at 256.626 kb on - strand, within GFF253at 256.706 kb on + strandat 256.706 kb on + strandat 256.706 kb on + strandat 256.706 kb on + strandat 256.706 kb on + strandat 256.706 kb on + strandat 256.711 kb on + strandat 256.714 kb on - strandat 256.714 kb on - strandat 256.714 kb on - strandat 256.716 kb on + strandat 256.716 kb on + strandat 256.719 kb on - strandat 256.748 kb on + strandat 256.748 kb on + strandat 256.804 kb on + strandat 256.874 kb on + strandat 256.874 kb on + strandat 256.882 kb on - strandat 256.892 kb on + strandat 256.893 kb on + strandat 256.900 kb on - strandat 256.900 kb on - strandat 256.901 kb on + strandat 256.901 kb on + strandat 256.907 kb on - strandat 256.909 kb on - strandat 256.929 kb on + strandat 256.929 kb on + strandat 256.929 kb on + strandat 256.934 kb on + strandat 256.942 kb on - strandat 256.946 kb on + strandat 256.946 kb on + strandat 256.953 kb on + strandat 256.954 kb on - strandat 257.031 kb on - strand, within GFF254at 257.041 kb on - strand, within GFF254at 257.046 kb on + strand, within GFF254at 257.054 kb on - strand, within GFF254at 257.054 kb on - strand, within GFF254at 257.091 kb on + strand, within GFF254at 257.114 kb on - strand, within GFF254at 257.139 kb on - strand, within GFF254at 257.139 kb on - strand, within GFF254at 257.139 kb on - strand, within GFF254at 257.175 kb on + strand, within GFF254at 257.181 kb on + strand, within GFF254at 257.193 kb on - strand, within GFF254at 257.275 kb on - strand, within GFF254at 257.346 kb on - strand, within GFF254at 257.346 kb on - strand, within GFF254at 257.404 kb on - strand, within GFF254at 257.422 kb on + strand, within GFF254at 257.430 kb on - strand, within GFF254at 257.430 kb on - strand, within GFF254at 257.466 kb on + strand, within GFF254at 257.466 kb on + strand, within GFF254at 257.469 kb on - strand, within GFF254at 257.474 kb on - strand, within GFF254at 257.474 kb on - strand, within GFF254at 257.476 kb on + strand, within GFF254at 257.484 kb on - strand, within GFF254at 257.501 kb on + strand, within GFF254at 257.537 kb on + strand, within GFF254at 257.545 kb on - strand, within GFF254at 257.560 kb on + strand, within GFF254at 257.560 kb on + strand, within GFF254at 257.560 kb on + strand, within GFF254at 257.565 kb on + strand, within GFF254at 257.568 kb on - strand, within GFF254at 257.568 kb on - strand, within GFF254at 257.570 kb on + strand, within GFF254at 257.578 kb on + strand, within GFF254at 257.578 kb on - strand, within GFF254at 257.602 kb on - strand, within GFF254at 257.604 kb on - strand, within GFF254at 257.630 kb on + strand, within GFF254at 257.630 kb on + strand, within GFF254at 257.630 kb on + strand, within GFF254at 257.635 kb on - strand, within GFF254at 257.650 kb on + strand, within GFF254at 257.650 kb on + strand, within GFF254at 257.650 kb on + strand, within GFF254at 257.650 kb on + strand, within GFF254at 257.650 kb on + strand, within GFF254at 257.650 kb on + strand, within GFF254at 257.650 kb on + strand, within GFF254at 257.657 kb on - strand, within GFF254at 257.658 kb on - strand, within GFF254at 257.668 kb on - strand, within GFF254at 257.669 kb on + strand, within GFF254at 257.693 kb on + strand, within GFF254at 257.693 kb on + strand, within GFF254

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Per-strain Table

Position Strand Gene LocusTag Fraction EMM_noCarbon with Sucrose and Putrescine
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255,597 + GFF251 0.33 +0.7
255,618 - GFF251 0.35 +0.5
255,635 + GFF251 0.37 -0.4
255,648 - GFF251 0.38 -1.0
255,673 - GFF251 0.41 -0.3
255,707 + GFF251 0.45 +0.3
255,745 + GFF251 0.49 -0.7
255,770 + GFF251 0.52 -0.9
255,810 + GFF251 0.56 -0.5
255,823 - GFF251 0.58 +1.6
255,833 - GFF251 0.59 +1.0
255,852 - GFF251 0.61 -0.9
255,862 - GFF251 0.62 +1.0
255,892 - GFF251 0.65 -0.5
255,902 - GFF251 0.66 -0.3
255,961 + GFF251 0.73 -0.7
255,984 + GFF251 0.76 +0.0
255,984 + GFF251 0.76 +2.0
255,984 + GFF251 0.76 -0.9
256,030 - GFF251 0.81 +0.1
256,035 - GFF251 0.81 -0.9
256,040 - GFF251 0.82 -0.2
256,053 - GFF251 0.83 -1.0
256,064 + GFF251 0.84 -0.5
256,093 - GFF251 0.88 +0.5
256,097 + GFF251 0.88 +1.0
256,103 + GFF251 0.89 +1.3
256,106 + GFF251 0.89 +0.4
256,114 - -0.0
256,124 - +1.0
256,128 + +0.3
256,133 + +0.9
256,138 + -1.5
256,187 + -1.6
256,195 - +3.3
256,206 - +0.1
256,217 - -0.7
256,229 - -0.3
256,239 - +0.2
256,239 - +0.5
256,241 + +0.8
256,241 + +0.6
256,249 - -0.6
256,249 - +2.6
256,256 + +0.6
256,272 + +0.2
256,277 + -1.7
256,280 - -0.4
256,290 + +0.3
256,297 + -2.6
256,298 - -1.1
256,298 - -0.5
256,315 - GFF252 0.12 -1.6
256,358 - GFF252 0.27 -0.2
256,359 - GFF252 0.27 +0.6
256,423 - GFF252 0.49 -0.8
256,449 + GFF252 0.58 +0.3
256,463 + GFF252 0.63 -0.4
256,473 + GFF252 0.66 -0.5
256,473 + GFF252 0.66 -0.1
256,482 + GFF252 0.69 -0.9
256,482 + GFF252 0.69 -0.1
256,482 + GFF252 0.69 +0.5
256,482 + GFF252 0.69 +1.6
256,482 + GFF252 0.69 +0.6
256,483 + GFF252 0.69 -0.1
256,483 + GFF252 0.69 -0.1
256,490 - GFF252 0.72 -0.3
256,490 - GFF252 0.72 -0.6
256,490 - GFF252 0.72 -1.5
256,490 - GFF252 0.72 +0.5
256,491 - GFF252 0.72 -0.5
256,507 + GFF252 0.78 +0.1
256,509 - GFF252 0.78 -0.2
256,519 - GFF252 0.82 -2.0
256,532 + GFF252 0.86 +0.7
256,532 - GFF252 0.86 +0.0
256,539 - GFF252 0.88 -0.9
256,539 - GFF252 0.88 +0.8
256,539 - GFF252 0.88 -1.3
256,540 - GFF252 0.89 +0.4
256,540 - GFF252 0.89 -0.6
256,541 + GFF252 0.89 +0.2
256,549 - -0.0
256,549 - -0.6
256,576 + -0.1
256,576 + +0.4
256,584 - +0.8
256,584 - +0.0
256,584 - -1.3
256,584 - +0.4
256,584 - -0.3
256,584 - +1.0
256,608 + GFF253 0.21 +1.0
256,616 - GFF253 0.26 +0.2
256,616 - GFF253 0.26 +0.3
256,618 + GFF253 0.28 +0.0
256,618 + GFF253 0.28 +0.0
256,618 + GFF253 0.28 +0.4
256,618 + GFF253 0.28 -0.2
256,618 + GFF253 0.28 -0.7
256,621 - GFF253 0.30 -2.0
256,621 - GFF253 0.30 +1.9
256,621 - GFF253 0.30 -0.2
256,621 - GFF253 0.30 -1.2
256,623 + GFF253 0.31 -0.1
256,623 + GFF253 0.31 +1.3
256,626 - GFF253 0.33 -0.5
256,626 - GFF253 0.33 -0.6
256,626 - GFF253 0.33 +1.1
256,626 - GFF253 0.33 +0.5
256,706 + -0.1
256,706 + +1.0
256,706 + +2.1
256,706 + +1.1
256,706 + -2.5
256,706 + +0.9
256,711 + +0.7
256,714 - +0.2
256,714 - -0.7
256,714 - +2.3
256,716 + +0.6
256,716 + +0.9
256,719 - +0.0
256,748 + -0.4
256,748 + +0.2
256,804 + -1.7
256,874 + -1.1
256,874 + +1.5
256,882 - -0.3
256,892 + +0.4
256,893 + +0.3
256,900 - +0.3
256,900 - -0.1
256,901 + -1.2
256,901 + +0.7
256,907 - +0.7
256,909 - +0.4
256,929 + +0.3
256,929 + +0.7
256,929 + +1.2
256,934 + -0.7
256,942 - -1.2
256,946 + -0.0
256,946 + +0.3
256,953 + +0.8
256,954 - -1.2
257,031 - GFF254 0.13 +0.8
257,041 - GFF254 0.14 +0.9
257,046 + GFF254 0.14 +0.0
257,054 - GFF254 0.15 -0.7
257,054 - GFF254 0.15 +0.3
257,091 + GFF254 0.17 +1.3
257,114 - GFF254 0.18 +0.5
257,139 - GFF254 0.20 -1.3
257,139 - GFF254 0.20 +1.0
257,139 - GFF254 0.20 -2.8
257,175 + GFF254 0.22 +0.9
257,181 + GFF254 0.22 -0.0
257,193 - GFF254 0.23 +0.1
257,275 - GFF254 0.28 -1.4
257,346 - GFF254 0.33 +1.2
257,346 - GFF254 0.33 +1.7
257,404 - GFF254 0.36 -1.4
257,422 + GFF254 0.37 -0.8
257,430 - GFF254 0.38 -0.2
257,430 - GFF254 0.38 -0.5
257,466 + GFF254 0.40 +0.9
257,466 + GFF254 0.40 +0.5
257,469 - GFF254 0.40 +0.6
257,474 - GFF254 0.41 -0.6
257,474 - GFF254 0.41 +0.4
257,476 + GFF254 0.41 +0.4
257,484 - GFF254 0.41 -0.5
257,501 + GFF254 0.42 -2.5
257,537 + GFF254 0.45 +0.9
257,545 - GFF254 0.45 -0.3
257,560 + GFF254 0.46 +1.3
257,560 + GFF254 0.46 -0.3
257,560 + GFF254 0.46 +1.0
257,565 + GFF254 0.46 -1.1
257,568 - GFF254 0.46 +1.1
257,568 - GFF254 0.46 -0.2
257,570 + GFF254 0.47 -0.6
257,578 + GFF254 0.47 -0.0
257,578 - GFF254 0.47 -1.7
257,602 - GFF254 0.49 +0.9
257,604 - GFF254 0.49 +1.2
257,630 + GFF254 0.50 +0.2
257,630 + GFF254 0.50 +0.7
257,630 + GFF254 0.50 -0.4
257,635 - GFF254 0.51 -0.5
257,650 + GFF254 0.52 -0.2
257,650 + GFF254 0.52 +0.2
257,650 + GFF254 0.52 +1.1
257,650 + GFF254 0.52 +0.3
257,650 + GFF254 0.52 -0.9
257,650 + GFF254 0.52 +0.6
257,650 + GFF254 0.52 -2.1
257,657 - GFF254 0.52 -1.8
257,658 - GFF254 0.52 +0.8
257,668 - GFF254 0.53 +1.3
257,669 + GFF254 0.53 -2.1
257,693 + GFF254 0.54 -0.6
257,693 + GFF254 0.54 -0.2

Or see this region's nucleotide sequence