Experiment: EMM_noCarbon with Sucrose and Putrescine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF241 and GFF242 are separated by 142 nucleotides GFF242 and GFF243 overlap by 8 nucleotides GFF243 and GFF244 overlap by 4 nucleotides
GFF241 - hypothetical protein, at 245,982 to 246,404
GFF241
GFF242 - Ribonuclease T2 family protein, at 246,547 to 247,254
GFF242
GFF243 - Quinolinate phosphoribosyltransferase [decarboxylating] (EC 2.4.2.19), at 247,247 to 248,101
GFF243
GFF244 - Prolyl endopeptidase (EC 3.4.21.26), at 248,098 to 250,224
GFF244
Position (kb)
247
248
249 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 246.253 kb on + strand, within GFF241 at 246.278 kb on + strand, within GFF241 at 246.286 kb on - strand, within GFF241 at 246.360 kb on - strand, within GFF241 at 246.368 kb on - strand at 246.457 kb on + strand at 246.457 kb on + strand at 246.457 kb on + strand at 246.465 kb on - strand at 246.467 kb on + strand at 246.467 kb on + strand at 246.475 kb on - strand at 246.477 kb on + strand at 246.485 kb on - strand at 246.499 kb on - strand at 246.511 kb on - strand at 246.543 kb on + strand at 246.543 kb on - strand at 246.552 kb on - strand at 246.563 kb on + strand at 246.563 kb on + strand at 246.581 kb on - strand at 246.595 kb on + strand at 246.635 kb on + strand, within GFF242 at 246.635 kb on + strand, within GFF242 at 246.640 kb on + strand, within GFF242 at 246.640 kb on + strand, within GFF242 at 246.640 kb on + strand, within GFF242 at 246.641 kb on - strand, within GFF242 at 246.648 kb on - strand, within GFF242 at 246.648 kb on - strand, within GFF242 at 246.648 kb on - strand, within GFF242 at 246.665 kb on - strand, within GFF242 at 246.704 kb on + strand, within GFF242 at 246.706 kb on - strand, within GFF242 at 246.709 kb on + strand, within GFF242 at 246.709 kb on + strand, within GFF242 at 246.712 kb on - strand, within GFF242 at 246.712 kb on - strand, within GFF242 at 246.727 kb on - strand, within GFF242 at 246.777 kb on + strand, within GFF242 at 246.785 kb on - strand, within GFF242 at 246.790 kb on - strand, within GFF242 at 246.792 kb on + strand, within GFF242 at 246.792 kb on + strand, within GFF242 at 246.795 kb on - strand, within GFF242 at 246.843 kb on + strand, within GFF242 at 246.858 kb on + strand, within GFF242 at 246.874 kb on - strand, within GFF242 at 246.885 kb on + strand, within GFF242 at 246.893 kb on - strand, within GFF242 at 246.899 kb on + strand, within GFF242 at 246.923 kb on - strand, within GFF242 at 246.962 kb on - strand, within GFF242 at 247.030 kb on + strand, within GFF242 at 247.077 kb on + strand, within GFF242 at 247.131 kb on + strand, within GFF242 at 247.169 kb on + strand, within GFF242 at 247.187 kb on + strand at 247.187 kb on + strand at 247.193 kb on + strand at 247.193 kb on + strand at 247.193 kb on + strand at 247.198 kb on + strand at 247.208 kb on + strand at 247.222 kb on - strand at 247.237 kb on - strand at 247.252 kb on - strand at 247.257 kb on + strand at 247.274 kb on + strand at 247.285 kb on + strand at 247.321 kb on - strand at 247.359 kb on - strand, within GFF243 at 247.371 kb on - strand, within GFF243 at 247.392 kb on + strand, within GFF243 at 247.428 kb on - strand, within GFF243 at 247.541 kb on + strand, within GFF243 at 247.592 kb on - strand, within GFF243 at 247.612 kb on - strand, within GFF243 at 247.612 kb on - strand, within GFF243 at 247.669 kb on + strand, within GFF243 at 247.680 kb on + strand, within GFF243 at 247.712 kb on + strand, within GFF243 at 247.717 kb on + strand, within GFF243 at 247.718 kb on - strand, within GFF243 at 247.728 kb on + strand, within GFF243 at 247.736 kb on - strand, within GFF243 at 247.771 kb on - strand, within GFF243 at 247.771 kb on - strand, within GFF243 at 247.771 kb on - strand, within GFF243 at 247.773 kb on + strand, within GFF243 at 247.775 kb on + strand, within GFF243 at 247.776 kb on - strand, within GFF243 at 247.782 kb on + strand, within GFF243 at 247.794 kb on + strand, within GFF243 at 247.802 kb on - strand, within GFF243 at 247.814 kb on - strand, within GFF243 at 247.818 kb on + strand, within GFF243 at 247.860 kb on + strand, within GFF243 at 247.879 kb on - strand, within GFF243 at 247.896 kb on - strand, within GFF243 at 247.925 kb on + strand, within GFF243 at 247.961 kb on - strand, within GFF243 at 247.966 kb on - strand, within GFF243 at 247.978 kb on - strand, within GFF243 at 248.019 kb on - strand at 248.034 kb on - strand at 248.063 kb on - strand at 248.068 kb on + strand at 248.125 kb on + strand at 248.142 kb on + strand at 248.143 kb on - strand at 248.266 kb on + strand at 248.274 kb on - strand at 248.274 kb on - strand at 248.279 kb on + strand at 248.290 kb on + strand at 248.328 kb on - strand, within GFF244 at 248.403 kb on - strand, within GFF244 at 248.443 kb on - strand, within GFF244 at 248.495 kb on - strand, within GFF244 at 248.515 kb on + strand, within GFF244 at 248.523 kb on - strand, within GFF244 at 248.523 kb on - strand, within GFF244 at 248.525 kb on - strand, within GFF244 at 248.576 kb on + strand, within GFF244 at 248.640 kb on + strand, within GFF244 at 248.702 kb on - strand, within GFF244 at 248.717 kb on + strand, within GFF244 at 248.717 kb on + strand, within GFF244 at 248.717 kb on + strand, within GFF244 at 248.717 kb on + strand, within GFF244 at 248.717 kb on + strand, within GFF244 at 248.717 kb on + strand, within GFF244 at 248.720 kb on - strand, within GFF244 at 248.725 kb on - strand, within GFF244 at 248.725 kb on - strand, within GFF244 at 248.725 kb on - strand, within GFF244 at 248.725 kb on - strand, within GFF244 at 248.725 kb on - strand, within GFF244 at 248.725 kb on - strand, within GFF244 at 248.757 kb on - strand, within GFF244 at 248.834 kb on + strand, within GFF244 at 248.862 kb on + strand, within GFF244 at 248.862 kb on + strand, within GFF244 at 248.862 kb on + strand, within GFF244 at 248.870 kb on - strand, within GFF244 at 248.870 kb on - strand, within GFF244 at 248.870 kb on - strand, within GFF244 at 248.885 kb on + strand, within GFF244 at 248.938 kb on - strand, within GFF244 at 248.962 kb on - strand, within GFF244 at 248.981 kb on - strand, within GFF244 at 248.982 kb on - strand, within GFF244 at 249.007 kb on - strand, within GFF244 at 249.007 kb on - strand, within GFF244 at 249.038 kb on + strand, within GFF244 at 249.054 kb on - strand, within GFF244
Per-strain Table
Position Strand Gene LocusTag Fraction EMM_noCarbon with Sucrose and Putrescine remove 246,253 + GFF241 0.64 -1.7 246,278 + GFF241 0.70 -0.1 246,286 - GFF241 0.72 +0.6 246,360 - GFF241 0.89 -0.9 246,368 - -0.4 246,457 + -1.1 246,457 + +0.3 246,457 + -1.8 246,465 - -0.6 246,467 + +1.7 246,467 + +0.3 246,475 - -0.4 246,477 + +1.2 246,485 - +0.4 246,499 - -0.8 246,511 - -1.2 246,543 + +1.9 246,543 - +1.6 246,552 - +0.7 246,563 + -0.3 246,563 + -1.2 246,581 - -0.4 246,595 + -2.4 246,635 + GFF242 0.12 -1.0 246,635 + GFF242 0.12 -0.0 246,640 + GFF242 0.13 -0.5 246,640 + GFF242 0.13 -0.8 246,640 + GFF242 0.13 -0.1 246,641 - GFF242 0.13 -1.6 246,648 - GFF242 0.14 -1.1 246,648 - GFF242 0.14 -0.4 246,648 - GFF242 0.14 +0.1 246,665 - GFF242 0.17 +1.2 246,704 + GFF242 0.22 -0.3 246,706 - GFF242 0.22 -0.8 246,709 + GFF242 0.23 +0.1 246,709 + GFF242 0.23 +0.4 246,712 - GFF242 0.23 -0.5 246,712 - GFF242 0.23 -2.0 246,727 - GFF242 0.25 -0.1 246,777 + GFF242 0.32 +1.0 246,785 - GFF242 0.34 +1.0 246,790 - GFF242 0.34 +0.9 246,792 + GFF242 0.35 +0.2 246,792 + GFF242 0.35 -1.1 246,795 - GFF242 0.35 -0.0 246,843 + GFF242 0.42 +1.0 246,858 + GFF242 0.44 -0.7 246,874 - GFF242 0.46 -0.8 246,885 + GFF242 0.48 -0.8 246,893 - GFF242 0.49 +0.0 246,899 + GFF242 0.50 +0.6 246,923 - GFF242 0.53 +0.3 246,962 - GFF242 0.59 -2.1 247,030 + GFF242 0.68 -0.3 247,077 + GFF242 0.75 -1.2 247,131 + GFF242 0.82 +0.0 247,169 + GFF242 0.88 -1.5 247,187 + +0.8 247,187 + +1.4 247,193 + -1.0 247,193 + -0.5 247,193 + -0.4 247,198 + -1.5 247,208 + +0.6 247,222 - +0.6 247,237 - -1.5 247,252 - -0.4 247,257 + -2.3 247,274 + -1.5 247,285 + -2.9 247,321 - -0.4 247,359 - GFF243 0.13 +0.1 247,371 - GFF243 0.15 -1.0 247,392 + GFF243 0.17 +0.1 247,428 - GFF243 0.21 -1.0 247,541 + GFF243 0.34 -0.7 247,592 - GFF243 0.40 -1.2 247,612 - GFF243 0.43 -1.0 247,612 - GFF243 0.43 +1.6 247,669 + GFF243 0.49 -2.0 247,680 + GFF243 0.51 -0.7 247,712 + GFF243 0.54 +0.3 247,717 + GFF243 0.55 -0.7 247,718 - GFF243 0.55 -0.4 247,728 + GFF243 0.56 +0.7 247,736 - GFF243 0.57 +0.7 247,771 - GFF243 0.61 -2.1 247,771 - GFF243 0.61 +0.3 247,771 - GFF243 0.61 -1.9 247,773 + GFF243 0.62 +1.4 247,775 + GFF243 0.62 -2.8 247,776 - GFF243 0.62 -1.7 247,782 + GFF243 0.63 +0.5 247,794 + GFF243 0.64 -1.7 247,802 - GFF243 0.65 -1.3 247,814 - GFF243 0.66 -0.8 247,818 + GFF243 0.67 -1.5 247,860 + GFF243 0.72 -1.7 247,879 - GFF243 0.74 -0.0 247,896 - GFF243 0.76 -0.7 247,925 + GFF243 0.79 -0.4 247,961 - GFF243 0.84 -3.0 247,966 - GFF243 0.84 -0.0 247,978 - GFF243 0.85 -0.8 248,019 - +0.2 248,034 - -0.6 248,063 - -1.8 248,068 + -1.6 248,125 + +1.2 248,142 + +0.5 248,143 - -0.1 248,266 + -0.2 248,274 - -0.2 248,274 - +0.2 248,279 + -0.7 248,290 + +0.6 248,328 - GFF244 0.11 -1.3 248,403 - GFF244 0.14 +0.9 248,443 - GFF244 0.16 -1.6 248,495 - GFF244 0.19 +0.0 248,515 + GFF244 0.20 -0.9 248,523 - GFF244 0.20 +0.1 248,523 - GFF244 0.20 -1.5 248,525 - GFF244 0.20 +0.4 248,576 + GFF244 0.22 -1.2 248,640 + GFF244 0.25 -0.4 248,702 - GFF244 0.28 +1.4 248,717 + GFF244 0.29 +0.5 248,717 + GFF244 0.29 -0.0 248,717 + GFF244 0.29 -0.3 248,717 + GFF244 0.29 +2.9 248,717 + GFF244 0.29 +1.2 248,717 + GFF244 0.29 -0.7 248,720 - GFF244 0.29 -0.7 248,725 - GFF244 0.29 +1.6 248,725 - GFF244 0.29 +0.4 248,725 - GFF244 0.29 +0.5 248,725 - GFF244 0.29 +0.4 248,725 - GFF244 0.29 +1.3 248,725 - GFF244 0.29 -2.0 248,757 - GFF244 0.31 -0.9 248,834 + GFF244 0.35 -0.8 248,862 + GFF244 0.36 +2.6 248,862 + GFF244 0.36 +0.9 248,862 + GFF244 0.36 -1.8 248,870 - GFF244 0.36 +0.7 248,870 - GFF244 0.36 -0.2 248,870 - GFF244 0.36 -0.7 248,885 + GFF244 0.37 -1.6 248,938 - GFF244 0.39 +2.0 248,962 - GFF244 0.41 +1.6 248,981 - GFF244 0.42 +0.6 248,982 - GFF244 0.42 -0.3 249,007 - GFF244 0.43 -0.2 249,007 - GFF244 0.43 -0.0 249,038 + GFF244 0.44 -0.7 249,054 - GFF244 0.45 +0.3
Or see this region's nucleotide sequence