Experiment: EMM_noCarbon with Sucrose and Putrescine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF231 and GFF232 are separated by 55 nucleotides GFF232 and GFF233 are separated by 121 nucleotides GFF233 and GFF234 are separated by 55 nucleotides GFF234 and GFF235 are separated by 34 nucleotides
GFF231 - Autolysin sensor kinase (EC 2.7.3.-), at 235,344 to 236,450
GFF231
GFF232 - Transcriptional regulator, HxlR family, at 236,506 to 236,988
GFF232
GFF233 - 'Guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase (EC 3.1.7.2) / GTP pyrophosphokinase (EC 2.7.6.5), (p)ppGpp synthetase II' transl_table=11, at 237,110 to 239,215
GFF233
GFF234 - hypothetical protein, at 239,271 to 239,558
GFF234
GFF235 - Phage protein (ACLAME 827), at 239,593 to 239,883
GFF235
Position (kb)
237
238
239
240 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 236.437 kb on + strand at 236.444 kb on - strand at 236.467 kb on - strand at 236.472 kb on - strand at 236.544 kb on - strand at 236.558 kb on + strand, within GFF232 at 236.558 kb on + strand, within GFF232 at 236.566 kb on - strand, within GFF232 at 236.566 kb on - strand, within GFF232 at 236.577 kb on + strand, within GFF232 at 236.577 kb on + strand, within GFF232 at 236.577 kb on + strand, within GFF232 at 236.577 kb on + strand, within GFF232 at 236.577 kb on + strand, within GFF232 at 236.585 kb on - strand, within GFF232 at 236.590 kb on + strand, within GFF232 at 236.590 kb on + strand, within GFF232 at 236.620 kb on + strand, within GFF232 at 236.628 kb on - strand, within GFF232 at 236.630 kb on + strand, within GFF232 at 236.638 kb on - strand, within GFF232 at 236.652 kb on + strand, within GFF232 at 236.674 kb on + strand, within GFF232 at 236.753 kb on + strand, within GFF232 at 236.785 kb on - strand, within GFF232 at 236.785 kb on - strand, within GFF232 at 236.832 kb on - strand, within GFF232 at 236.863 kb on - strand, within GFF232 at 236.912 kb on + strand, within GFF232 at 236.915 kb on - strand, within GFF232 at 236.915 kb on - strand, within GFF232 at 236.925 kb on - strand, within GFF232 at 236.925 kb on - strand, within GFF232 at 236.926 kb on + strand, within GFF232 at 236.958 kb on + strand at 236.964 kb on - strand at 237.080 kb on + strand at 237.115 kb on + strand at 237.120 kb on + strand at 237.139 kb on + strand at 237.183 kb on - strand at 237.208 kb on - strand at 237.208 kb on - strand at 237.238 kb on + strand at 237.267 kb on + strand at 237.322 kb on + strand, within GFF233 at 237.346 kb on + strand, within GFF233 at 237.351 kb on + strand, within GFF233 at 237.351 kb on + strand, within GFF233 at 237.359 kb on - strand, within GFF233 at 237.359 kb on - strand, within GFF233 at 237.418 kb on + strand, within GFF233 at 237.418 kb on + strand, within GFF233 at 237.424 kb on + strand, within GFF233 at 237.424 kb on + strand, within GFF233 at 237.426 kb on - strand, within GFF233 at 237.432 kb on - strand, within GFF233 at 237.546 kb on + strand, within GFF233 at 237.556 kb on - strand, within GFF233 at 237.564 kb on - strand, within GFF233 at 237.606 kb on + strand, within GFF233 at 237.612 kb on - strand, within GFF233 at 237.673 kb on - strand, within GFF233 at 237.692 kb on + strand, within GFF233 at 237.692 kb on + strand, within GFF233 at 237.700 kb on - strand, within GFF233 at 237.700 kb on - strand, within GFF233 at 237.700 kb on - strand, within GFF233 at 237.700 kb on - strand, within GFF233 at 237.700 kb on - strand, within GFF233 at 237.710 kb on - strand, within GFF233 at 237.724 kb on + strand, within GFF233 at 237.724 kb on + strand, within GFF233 at 237.735 kb on - strand, within GFF233 at 237.735 kb on - strand, within GFF233 at 237.742 kb on + strand, within GFF233 at 237.742 kb on + strand, within GFF233 at 237.789 kb on + strand, within GFF233 at 237.789 kb on + strand, within GFF233 at 237.789 kb on + strand, within GFF233 at 237.789 kb on + strand, within GFF233 at 237.807 kb on - strand, within GFF233 at 237.870 kb on + strand, within GFF233 at 237.870 kb on + strand, within GFF233 at 237.870 kb on + strand, within GFF233 at 237.870 kb on + strand, within GFF233 at 237.873 kb on - strand, within GFF233 at 237.878 kb on - strand, within GFF233 at 237.878 kb on - strand, within GFF233 at 237.886 kb on - strand, within GFF233 at 237.889 kb on + strand, within GFF233 at 237.953 kb on + strand, within GFF233 at 237.982 kb on + strand, within GFF233 at 237.990 kb on - strand, within GFF233 at 237.990 kb on - strand, within GFF233 at 238.070 kb on + strand, within GFF233 at 238.070 kb on + strand, within GFF233 at 238.078 kb on - strand, within GFF233 at 238.194 kb on + strand, within GFF233 at 238.212 kb on + strand, within GFF233 at 238.225 kb on + strand, within GFF233 at 238.295 kb on + strand, within GFF233 at 238.308 kb on + strand, within GFF233 at 238.330 kb on - strand, within GFF233 at 238.350 kb on - strand, within GFF233 at 238.399 kb on - strand, within GFF233 at 238.465 kb on - strand, within GFF233 at 238.476 kb on + strand, within GFF233 at 238.601 kb on - strand, within GFF233 at 238.623 kb on - strand, within GFF233 at 238.663 kb on - strand, within GFF233 at 238.715 kb on + strand, within GFF233 at 238.779 kb on + strand, within GFF233 at 238.803 kb on + strand, within GFF233 at 238.803 kb on + strand, within GFF233 at 238.811 kb on - strand, within GFF233 at 238.908 kb on + strand, within GFF233 at 238.942 kb on - strand, within GFF233 at 238.955 kb on + strand, within GFF233 at 238.975 kb on + strand, within GFF233 at 238.975 kb on + strand, within GFF233 at 239.004 kb on - strand, within GFF233 at 239.097 kb on - strand at 239.102 kb on + strand at 239.115 kb on - strand at 239.127 kb on + strand at 239.127 kb on + strand at 239.135 kb on - strand at 239.185 kb on + strand at 239.200 kb on - strand at 239.218 kb on - strand at 239.241 kb on + strand at 239.241 kb on + strand at 239.326 kb on - strand, within GFF234 at 239.348 kb on + strand, within GFF234 at 239.356 kb on - strand, within GFF234 at 239.587 kb on - strand at 239.593 kb on - strand at 239.599 kb on - strand at 239.599 kb on - strand at 239.609 kb on + strand at 239.609 kb on + strand at 239.609 kb on + strand at 239.609 kb on + strand at 239.617 kb on - strand at 239.617 kb on - strand at 239.624 kb on - strand, within GFF235 at 239.624 kb on - strand, within GFF235 at 239.630 kb on - strand, within GFF235 at 239.642 kb on + strand, within GFF235 at 239.665 kb on - strand, within GFF235 at 239.665 kb on - strand, within GFF235 at 239.668 kb on - strand, within GFF235 at 239.679 kb on + strand, within GFF235 at 239.687 kb on - strand, within GFF235 at 239.753 kb on - strand, within GFF235 at 239.768 kb on + strand, within GFF235 at 239.770 kb on + strand, within GFF235 at 239.776 kb on - strand, within GFF235 at 239.778 kb on - strand, within GFF235 at 239.810 kb on + strand, within GFF235 at 239.841 kb on + strand, within GFF235 at 239.849 kb on - strand, within GFF235 at 239.849 kb on - strand, within GFF235 at 239.868 kb on - strand at 239.869 kb on + strand at 239.915 kb on + strand at 239.924 kb on + strand at 239.952 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction EMM_noCarbon with Sucrose and Putrescine remove 236,437 + -0.3 236,444 - -1.3 236,467 - -1.2 236,472 - +0.1 236,544 - -0.6 236,558 + GFF232 0.11 +0.3 236,558 + GFF232 0.11 -0.3 236,566 - GFF232 0.12 +0.1 236,566 - GFF232 0.12 +0.7 236,577 + GFF232 0.15 -0.5 236,577 + GFF232 0.15 -1.0 236,577 + GFF232 0.15 +0.3 236,577 + GFF232 0.15 -0.4 236,577 + GFF232 0.15 +0.3 236,585 - GFF232 0.16 -1.0 236,590 + GFF232 0.17 +0.8 236,590 + GFF232 0.17 -0.7 236,620 + GFF232 0.24 +0.0 236,628 - GFF232 0.25 -1.0 236,630 + GFF232 0.26 -0.9 236,638 - GFF232 0.27 -0.3 236,652 + GFF232 0.30 +0.5 236,674 + GFF232 0.35 -0.5 236,753 + GFF232 0.51 -1.2 236,785 - GFF232 0.58 +1.0 236,785 - GFF232 0.58 +0.6 236,832 - GFF232 0.67 +1.1 236,863 - GFF232 0.74 +1.7 236,912 + GFF232 0.84 -0.2 236,915 - GFF232 0.85 -0.7 236,915 - GFF232 0.85 +1.4 236,925 - GFF232 0.87 -1.7 236,925 - GFF232 0.87 +0.3 236,926 + GFF232 0.87 +0.3 236,958 + +0.8 236,964 - -0.8 237,080 + +1.3 237,115 + -1.6 237,120 + -3.6 237,139 + -1.3 237,183 - -1.2 237,208 - -1.5 237,208 - -2.5 237,238 + -1.8 237,267 + -2.9 237,322 + GFF233 0.10 -2.8 237,346 + GFF233 0.11 +0.5 237,351 + GFF233 0.11 -2.0 237,351 + GFF233 0.11 -1.1 237,359 - GFF233 0.12 -2.4 237,359 - GFF233 0.12 -1.3 237,418 + GFF233 0.15 -2.2 237,418 + GFF233 0.15 -1.1 237,424 + GFF233 0.15 -0.6 237,424 + GFF233 0.15 +1.1 237,426 - GFF233 0.15 -1.9 237,432 - GFF233 0.15 -1.6 237,546 + GFF233 0.21 -1.6 237,556 - GFF233 0.21 -1.7 237,564 - GFF233 0.22 -1.7 237,606 + GFF233 0.24 -0.5 237,612 - GFF233 0.24 -2.6 237,673 - GFF233 0.27 -2.6 237,692 + GFF233 0.28 -1.6 237,692 + GFF233 0.28 -0.5 237,700 - GFF233 0.28 -2.8 237,700 - GFF233 0.28 -3.4 237,700 - GFF233 0.28 -3.1 237,700 - GFF233 0.28 -1.8 237,700 - GFF233 0.28 -2.2 237,710 - GFF233 0.28 -1.2 237,724 + GFF233 0.29 -2.4 237,724 + GFF233 0.29 -1.5 237,735 - GFF233 0.30 -3.2 237,735 - GFF233 0.30 -1.6 237,742 + GFF233 0.30 -2.6 237,742 + GFF233 0.30 -1.9 237,789 + GFF233 0.32 -1.6 237,789 + GFF233 0.32 -2.3 237,789 + GFF233 0.32 +0.0 237,789 + GFF233 0.32 -2.7 237,807 - GFF233 0.33 -0.8 237,870 + GFF233 0.36 -1.5 237,870 + GFF233 0.36 -2.5 237,870 + GFF233 0.36 -3.8 237,870 + GFF233 0.36 -0.9 237,873 - GFF233 0.36 -2.4 237,878 - GFF233 0.36 -0.2 237,878 - GFF233 0.36 -2.2 237,886 - GFF233 0.37 -1.5 237,889 + GFF233 0.37 -1.4 237,953 + GFF233 0.40 -2.1 237,982 + GFF233 0.41 -2.6 237,990 - GFF233 0.42 -2.2 237,990 - GFF233 0.42 -1.0 238,070 + GFF233 0.46 -0.9 238,070 + GFF233 0.46 -3.7 238,078 - GFF233 0.46 -2.5 238,194 + GFF233 0.51 -0.3 238,212 + GFF233 0.52 -2.2 238,225 + GFF233 0.53 +0.1 238,295 + GFF233 0.56 -1.0 238,308 + GFF233 0.57 -1.2 238,330 - GFF233 0.58 -2.9 238,350 - GFF233 0.59 -2.4 238,399 - GFF233 0.61 -2.6 238,465 - GFF233 0.64 -0.9 238,476 + GFF233 0.65 -3.6 238,601 - GFF233 0.71 -1.4 238,623 - GFF233 0.72 -3.4 238,663 - GFF233 0.74 -3.0 238,715 + GFF233 0.76 -2.8 238,779 + GFF233 0.79 -2.6 238,803 + GFF233 0.80 -0.3 238,803 + GFF233 0.80 +0.6 238,811 - GFF233 0.81 -2.2 238,908 + GFF233 0.85 +0.7 238,942 - GFF233 0.87 +0.7 238,955 + GFF233 0.88 -0.5 238,975 + GFF233 0.89 -1.6 238,975 + GFF233 0.89 +0.2 239,004 - GFF233 0.90 -3.5 239,097 - -1.4 239,102 + -0.8 239,115 - -3.0 239,127 + +0.3 239,127 + -1.5 239,135 - -2.8 239,185 + -1.8 239,200 - -1.2 239,218 - -0.5 239,241 + -0.5 239,241 + +0.7 239,326 - GFF234 0.19 -1.7 239,348 + GFF234 0.27 -2.6 239,356 - GFF234 0.30 +0.6 239,587 - +0.7 239,593 - -0.4 239,599 - +0.2 239,599 - +1.2 239,609 + -2.3 239,609 + +0.6 239,609 + +1.5 239,609 + -0.8 239,617 - -1.4 239,617 - +0.4 239,624 - GFF235 0.11 -1.5 239,624 - GFF235 0.11 -1.6 239,630 - GFF235 0.13 +0.1 239,642 + GFF235 0.17 +0.1 239,665 - GFF235 0.25 -0.8 239,665 - GFF235 0.25 +0.7 239,668 - GFF235 0.26 +0.5 239,679 + GFF235 0.30 +0.5 239,687 - GFF235 0.32 +0.2 239,753 - GFF235 0.55 +0.8 239,768 + GFF235 0.60 -0.8 239,770 + GFF235 0.61 -1.4 239,776 - GFF235 0.63 +0.4 239,778 - GFF235 0.64 +0.4 239,810 + GFF235 0.75 -1.2 239,841 + GFF235 0.85 -0.0 239,849 - GFF235 0.88 +1.0 239,849 - GFF235 0.88 -0.6 239,868 - +1.2 239,869 + -0.9 239,915 + -0.4 239,924 + +0.1 239,952 + +0.6
Or see this region's nucleotide sequence