Experiment: EMM_noCarbon with Sucrose and Putrescine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2297 and GFF2298 are separated by 66 nucleotides GFF2298 and GFF2299 are separated by 5 nucleotides GFF2299 and GFF2300 are separated by 71 nucleotides GFF2300 and GFF2301 are separated by 102 nucleotides GFF2301 and GFF2302 are separated by 32 nucleotides GFF2302 and GFF2303 overlap by 26 nucleotides
GFF2297 - hypothetical protein, at 2,324,955 to 2,326,709
GFF2297
GFF2298 - hypothetical protein, at 2,326,776 to 2,326,979
GFF2298
GFF2299 - UPF0391 membrane protein RPC_2356, at 2,326,985 to 2,327,170
GFF2299
GFF2300 - Glyoxylate reductase (EC 1.1.1.79) @ Glyoxylate reductase (EC 1.1.1.26) @ Hydroxypyruvate reductase (EC 1.1.1.81), at 2,327,242 to 2,328,240
GFF2300
GFF2301 - hypothetical protein, at 2,328,343 to 2,328,840
GFF2301
GFF2302 - hypothetical protein, at 2,328,873 to 2,329,187
GFF2302
GFF2303 - hypothetical protein, at 2,329,162 to 2,330,313
GFF2303
Position (kb)
2327
2328
2329 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2326.348 kb on + strand, within GFF2297 at 2326.348 kb on + strand, within GFF2297 at 2326.348 kb on + strand, within GFF2297 at 2326.358 kb on + strand, within GFF2297 at 2326.358 kb on + strand, within GFF2297 at 2326.408 kb on + strand, within GFF2297 at 2326.408 kb on + strand, within GFF2297 at 2326.413 kb on + strand, within GFF2297 at 2326.413 kb on + strand, within GFF2297 at 2326.413 kb on + strand, within GFF2297 at 2326.416 kb on - strand, within GFF2297 at 2326.416 kb on - strand, within GFF2297 at 2326.421 kb on - strand, within GFF2297 at 2326.421 kb on - strand, within GFF2297 at 2326.446 kb on + strand, within GFF2297 at 2326.446 kb on + strand, within GFF2297 at 2326.456 kb on + strand, within GFF2297 at 2326.459 kb on - strand, within GFF2297 at 2326.464 kb on - strand, within GFF2297 at 2326.464 kb on - strand, within GFF2297 at 2326.476 kb on - strand, within GFF2297 at 2326.488 kb on + strand, within GFF2297 at 2326.488 kb on + strand, within GFF2297 at 2326.496 kb on - strand, within GFF2297 at 2326.496 kb on - strand, within GFF2297 at 2326.498 kb on + strand, within GFF2297 at 2326.498 kb on + strand, within GFF2297 at 2326.506 kb on - strand, within GFF2297 at 2326.506 kb on - strand, within GFF2297 at 2326.506 kb on - strand, within GFF2297 at 2326.506 kb on - strand, within GFF2297 at 2326.506 kb on - strand, within GFF2297 at 2326.511 kb on - strand, within GFF2297 at 2326.511 kb on - strand, within GFF2297 at 2326.511 kb on - strand, within GFF2297 at 2326.530 kb on - strand, within GFF2297 at 2326.540 kb on - strand at 2326.580 kb on + strand at 2326.590 kb on + strand at 2326.646 kb on - strand at 2326.646 kb on - strand at 2326.648 kb on + strand at 2326.648 kb on + strand at 2326.648 kb on + strand at 2326.654 kb on + strand at 2326.656 kb on - strand at 2326.712 kb on + strand at 2326.784 kb on + strand at 2326.809 kb on + strand, within GFF2298 at 2326.885 kb on - strand, within GFF2298 at 2326.926 kb on - strand, within GFF2298 at 2326.948 kb on + strand, within GFF2298 at 2327.000 kb on + strand at 2327.000 kb on + strand at 2327.023 kb on + strand, within GFF2299 at 2327.061 kb on + strand, within GFF2299 at 2327.093 kb on + strand, within GFF2299 at 2327.240 kb on + strand at 2327.248 kb on - strand at 2327.253 kb on - strand at 2327.259 kb on - strand at 2327.335 kb on + strand at 2327.370 kb on + strand, within GFF2300 at 2327.370 kb on + strand, within GFF2300 at 2327.378 kb on - strand, within GFF2300 at 2327.378 kb on - strand, within GFF2300 at 2327.428 kb on - strand, within GFF2300 at 2327.435 kb on - strand, within GFF2300 at 2327.435 kb on - strand, within GFF2300 at 2327.463 kb on + strand, within GFF2300 at 2327.507 kb on + strand, within GFF2300 at 2327.522 kb on + strand, within GFF2300 at 2327.536 kb on - strand, within GFF2300 at 2327.547 kb on + strand, within GFF2300 at 2327.810 kb on - strand, within GFF2300 at 2327.810 kb on - strand, within GFF2300 at 2327.819 kb on - strand, within GFF2300 at 2327.820 kb on - strand, within GFF2300 at 2327.824 kb on + strand, within GFF2300 at 2327.824 kb on + strand, within GFF2300 at 2327.824 kb on + strand, within GFF2300 at 2327.829 kb on + strand, within GFF2300 at 2327.829 kb on + strand, within GFF2300 at 2327.832 kb on - strand, within GFF2300 at 2327.834 kb on + strand, within GFF2300 at 2327.837 kb on - strand, within GFF2300 at 2327.837 kb on - strand, within GFF2300 at 2327.848 kb on + strand, within GFF2300 at 2327.852 kb on - strand, within GFF2300 at 2327.856 kb on + strand, within GFF2300 at 2327.864 kb on - strand, within GFF2300 at 2327.864 kb on - strand, within GFF2300 at 2327.874 kb on + strand, within GFF2300 at 2327.876 kb on + strand, within GFF2300 at 2327.881 kb on + strand, within GFF2300 at 2327.884 kb on - strand, within GFF2300 at 2327.884 kb on - strand, within GFF2300 at 2327.889 kb on - strand, within GFF2300 at 2327.889 kb on - strand, within GFF2300 at 2327.906 kb on - strand, within GFF2300 at 2327.948 kb on + strand, within GFF2300 at 2327.948 kb on + strand, within GFF2300 at 2327.949 kb on - strand, within GFF2300 at 2327.956 kb on - strand, within GFF2300 at 2327.956 kb on - strand, within GFF2300 at 2327.956 kb on - strand, within GFF2300 at 2327.956 kb on - strand, within GFF2300 at 2327.956 kb on - strand, within GFF2300 at 2327.971 kb on + strand, within GFF2300 at 2327.979 kb on + strand, within GFF2300 at 2327.979 kb on - strand, within GFF2300 at 2328.019 kb on + strand, within GFF2300 at 2328.034 kb on + strand, within GFF2300 at 2328.034 kb on + strand, within GFF2300 at 2328.104 kb on + strand, within GFF2300 at 2328.115 kb on + strand, within GFF2300 at 2328.123 kb on - strand, within GFF2300 at 2328.134 kb on + strand, within GFF2300 at 2328.154 kb on - strand at 2328.156 kb on - strand at 2328.179 kb on - strand at 2328.179 kb on - strand at 2328.179 kb on - strand at 2328.179 kb on - strand at 2328.221 kb on - strand at 2328.248 kb on - strand at 2328.258 kb on + strand at 2328.266 kb on - strand at 2328.280 kb on + strand at 2328.298 kb on + strand at 2328.305 kb on - strand at 2328.844 kb on - strand at 2328.986 kb on - strand, within GFF2302 at 2328.997 kb on + strand, within GFF2302 at 2328.997 kb on + strand, within GFF2302 at 2328.997 kb on + strand, within GFF2302 at 2329.005 kb on - strand, within GFF2302 at 2329.005 kb on - strand, within GFF2302 at 2329.005 kb on - strand, within GFF2302 at 2329.007 kb on - strand, within GFF2302 at 2329.057 kb on + strand, within GFF2302 at 2329.141 kb on + strand, within GFF2302 at 2329.148 kb on - strand, within GFF2302 at 2329.148 kb on - strand, within GFF2302 at 2329.153 kb on - strand, within GFF2302 at 2329.165 kb on + strand at 2329.165 kb on + strand at 2329.172 kb on - strand at 2329.172 kb on - strand at 2329.173 kb on - strand at 2329.173 kb on - strand at 2329.178 kb on - strand at 2329.183 kb on - strand at 2329.207 kb on + strand at 2329.213 kb on + strand at 2329.218 kb on + strand at 2329.221 kb on + strand at 2329.226 kb on - strand at 2329.228 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction EMM_noCarbon with Sucrose and Putrescine remove 2,326,348 + GFF2297 0.79 -0.1 2,326,348 + GFF2297 0.79 +0.6 2,326,348 + GFF2297 0.79 -0.2 2,326,358 + GFF2297 0.80 +0.2 2,326,358 + GFF2297 0.80 +0.5 2,326,408 + GFF2297 0.83 -0.0 2,326,408 + GFF2297 0.83 -1.2 2,326,413 + GFF2297 0.83 +0.9 2,326,413 + GFF2297 0.83 +0.3 2,326,413 + GFF2297 0.83 +0.0 2,326,416 - GFF2297 0.83 -0.1 2,326,416 - GFF2297 0.83 -0.7 2,326,421 - GFF2297 0.84 -0.3 2,326,421 - GFF2297 0.84 -0.6 2,326,446 + GFF2297 0.85 +1.3 2,326,446 + GFF2297 0.85 -0.3 2,326,456 + GFF2297 0.86 -0.5 2,326,459 - GFF2297 0.86 -3.4 2,326,464 - GFF2297 0.86 +0.7 2,326,464 - GFF2297 0.86 +0.1 2,326,476 - GFF2297 0.87 +0.8 2,326,488 + GFF2297 0.87 -0.0 2,326,488 + GFF2297 0.87 -0.0 2,326,496 - GFF2297 0.88 -1.2 2,326,496 - GFF2297 0.88 +1.5 2,326,498 + GFF2297 0.88 +1.3 2,326,498 + GFF2297 0.88 -0.5 2,326,506 - GFF2297 0.88 -0.8 2,326,506 - GFF2297 0.88 +0.9 2,326,506 - GFF2297 0.88 -1.2 2,326,506 - GFF2297 0.88 -1.2 2,326,506 - GFF2297 0.88 +0.6 2,326,511 - GFF2297 0.89 -1.0 2,326,511 - GFF2297 0.89 -1.0 2,326,511 - GFF2297 0.89 +1.1 2,326,530 - GFF2297 0.90 +0.7 2,326,540 - +0.3 2,326,580 + +0.1 2,326,590 + +0.2 2,326,646 - +0.1 2,326,646 - -0.5 2,326,648 + +0.3 2,326,648 + +0.2 2,326,648 + +2.2 2,326,654 + -0.6 2,326,656 - -1.3 2,326,712 + -0.4 2,326,784 + +0.4 2,326,809 + GFF2298 0.16 +1.3 2,326,885 - GFF2298 0.53 +0.7 2,326,926 - GFF2298 0.74 -0.7 2,326,948 + GFF2298 0.84 -1.4 2,327,000 + +0.8 2,327,000 + +1.5 2,327,023 + GFF2299 0.20 -0.3 2,327,061 + GFF2299 0.41 +0.8 2,327,093 + GFF2299 0.58 -1.2 2,327,240 + -0.0 2,327,248 - -0.6 2,327,253 - +1.5 2,327,259 - +0.9 2,327,335 + +0.7 2,327,370 + GFF2300 0.13 -0.4 2,327,370 + GFF2300 0.13 -0.0 2,327,378 - GFF2300 0.14 -0.9 2,327,378 - GFF2300 0.14 -0.2 2,327,428 - GFF2300 0.19 -2.4 2,327,435 - GFF2300 0.19 -0.4 2,327,435 - GFF2300 0.19 +1.0 2,327,463 + GFF2300 0.22 +0.9 2,327,507 + GFF2300 0.27 -0.9 2,327,522 + GFF2300 0.28 -0.1 2,327,536 - GFF2300 0.29 +0.2 2,327,547 + GFF2300 0.31 +0.3 2,327,810 - GFF2300 0.57 +0.4 2,327,810 - GFF2300 0.57 +0.7 2,327,819 - GFF2300 0.58 -2.0 2,327,820 - GFF2300 0.58 -1.2 2,327,824 + GFF2300 0.58 -0.0 2,327,824 + GFF2300 0.58 -0.5 2,327,824 + GFF2300 0.58 +0.1 2,327,829 + GFF2300 0.59 -1.2 2,327,829 + GFF2300 0.59 +0.5 2,327,832 - GFF2300 0.59 -1.2 2,327,834 + GFF2300 0.59 +0.8 2,327,837 - GFF2300 0.60 -1.6 2,327,837 - GFF2300 0.60 -0.4 2,327,848 + GFF2300 0.61 -0.9 2,327,852 - GFF2300 0.61 +1.0 2,327,856 + GFF2300 0.61 +1.0 2,327,864 - GFF2300 0.62 -0.2 2,327,864 - GFF2300 0.62 -0.8 2,327,874 + GFF2300 0.63 +1.5 2,327,876 + GFF2300 0.63 +1.1 2,327,881 + GFF2300 0.64 +0.1 2,327,884 - GFF2300 0.64 -0.4 2,327,884 - GFF2300 0.64 -0.9 2,327,889 - GFF2300 0.65 -0.6 2,327,889 - GFF2300 0.65 +1.3 2,327,906 - GFF2300 0.66 +1.2 2,327,948 + GFF2300 0.71 -1.0 2,327,948 + GFF2300 0.71 -0.2 2,327,949 - GFF2300 0.71 -0.1 2,327,956 - GFF2300 0.71 +0.3 2,327,956 - GFF2300 0.71 +1.8 2,327,956 - GFF2300 0.71 -1.2 2,327,956 - GFF2300 0.71 -0.5 2,327,956 - GFF2300 0.71 -0.2 2,327,971 + GFF2300 0.73 +0.3 2,327,979 + GFF2300 0.74 +0.4 2,327,979 - GFF2300 0.74 -1.4 2,328,019 + GFF2300 0.78 +2.1 2,328,034 + GFF2300 0.79 +1.3 2,328,034 + GFF2300 0.79 -0.0 2,328,104 + GFF2300 0.86 +0.1 2,328,115 + GFF2300 0.87 +0.7 2,328,123 - GFF2300 0.88 +1.3 2,328,134 + GFF2300 0.89 -0.6 2,328,154 - -0.6 2,328,156 - -0.2 2,328,179 - -0.0 2,328,179 - +0.4 2,328,179 - +0.3 2,328,179 - +0.9 2,328,221 - -2.0 2,328,248 - -0.4 2,328,258 + +1.5 2,328,266 - +1.8 2,328,280 + -2.9 2,328,298 + +0.1 2,328,305 - -1.9 2,328,844 - -0.0 2,328,986 - GFF2302 0.36 +0.8 2,328,997 + GFF2302 0.39 -2.2 2,328,997 + GFF2302 0.39 -0.9 2,328,997 + GFF2302 0.39 -0.9 2,329,005 - GFF2302 0.42 +0.1 2,329,005 - GFF2302 0.42 +0.5 2,329,005 - GFF2302 0.42 +0.4 2,329,007 - GFF2302 0.43 +0.4 2,329,057 + GFF2302 0.58 +0.6 2,329,141 + GFF2302 0.85 -1.4 2,329,148 - GFF2302 0.87 -2.6 2,329,148 - GFF2302 0.87 -0.1 2,329,153 - GFF2302 0.89 +0.9 2,329,165 + -0.0 2,329,165 + -0.4 2,329,172 - -0.9 2,329,172 - +0.3 2,329,173 - +0.1 2,329,173 - -0.0 2,329,178 - +0.4 2,329,183 - -0.8 2,329,207 + -0.9 2,329,213 + -0.2 2,329,218 + +0.7 2,329,221 + +0.3 2,329,226 - -0.9 2,329,228 - -0.6
Or see this region's nucleotide sequence