Experiment: EMM_noCarbon with Sucrose and Putrescine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2268 and GFF2269 overlap by 41 nucleotides GFF2269 and GFF2270 overlap by 4 nucleotides GFF2270 and GFF2271 are separated by 71 nucleotides GFF2271 and GFF2272 are separated by 224 nucleotides GFF2272 and GFF2273 are separated by 61 nucleotides
GFF2268 - Transcriptional regulator, MarR family, at 2,297,381 to 2,297,788
GFF2268
GFF2269 - NLP/P60 family lipoprotein, at 2,297,748 to 2,298,656
GFF2269
GFF2270 - N-acetyl-gamma-glutamyl-phosphate reductase (EC 1.2.1.38), at 2,298,653 to 2,299,597
GFF2270
GFF2271 - hypothetical protein, at 2,299,669 to 2,299,878
GFF2271
GFF2272 - hypothetical protein, at 2,300,103 to 2,300,273
GFF2272
GFF2273 - L-sorbosone dehydrogenase, at 2,300,335 to 2,301,663
GFF2273
Position (kb)
2298
2299
2300 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2297.754 kb on + strand at 2297.754 kb on + strand at 2297.759 kb on + strand at 2297.759 kb on + strand at 2297.767 kb on - strand at 2297.767 kb on - strand at 2297.767 kb on - strand at 2297.767 kb on - strand at 2297.767 kb on - strand at 2297.767 kb on - strand at 2297.767 kb on - strand at 2297.767 kb on - strand at 2297.823 kb on - strand at 2297.823 kb on - strand at 2297.916 kb on + strand, within GFF2269 at 2297.916 kb on + strand, within GFF2269 at 2297.929 kb on - strand, within GFF2269 at 2297.939 kb on - strand, within GFF2269 at 2297.959 kb on - strand, within GFF2269 at 2297.959 kb on - strand, within GFF2269 at 2297.976 kb on - strand, within GFF2269 at 2298.043 kb on - strand, within GFF2269 at 2298.054 kb on + strand, within GFF2269 at 2298.077 kb on - strand, within GFF2269 at 2298.146 kb on - strand, within GFF2269 at 2298.151 kb on - strand, within GFF2269 at 2298.151 kb on - strand, within GFF2269 at 2298.213 kb on + strand, within GFF2269 at 2298.301 kb on + strand, within GFF2269 at 2298.316 kb on + strand, within GFF2269 at 2298.316 kb on + strand, within GFF2269 at 2298.316 kb on + strand, within GFF2269 at 2298.316 kb on + strand, within GFF2269 at 2298.324 kb on - strand, within GFF2269 at 2298.324 kb on - strand, within GFF2269 at 2298.326 kb on + strand, within GFF2269 at 2298.334 kb on - strand, within GFF2269 at 2298.356 kb on - strand, within GFF2269 at 2298.375 kb on - strand, within GFF2269 at 2298.375 kb on - strand, within GFF2269 at 2298.375 kb on - strand, within GFF2269 at 2298.383 kb on + strand, within GFF2269 at 2298.387 kb on + strand, within GFF2269 at 2298.426 kb on + strand, within GFF2269 at 2298.434 kb on - strand, within GFF2269 at 2298.471 kb on + strand, within GFF2269 at 2298.471 kb on + strand, within GFF2269 at 2298.526 kb on + strand, within GFF2269 at 2298.532 kb on + strand, within GFF2269 at 2298.540 kb on - strand, within GFF2269 at 2298.545 kb on + strand, within GFF2269 at 2298.545 kb on + strand, within GFF2269 at 2298.553 kb on - strand, within GFF2269 at 2298.586 kb on + strand at 2298.613 kb on + strand at 2298.613 kb on + strand at 2298.613 kb on + strand at 2298.621 kb on - strand at 2298.643 kb on + strand at 2298.643 kb on + strand at 2298.646 kb on - strand at 2298.651 kb on - strand at 2298.651 kb on - strand at 2298.688 kb on - strand at 2298.688 kb on - strand at 2298.726 kb on + strand at 2298.726 kb on + strand at 2298.734 kb on - strand at 2298.734 kb on - strand at 2298.750 kb on - strand, within GFF2270 at 2298.764 kb on + strand, within GFF2270 at 2298.764 kb on + strand, within GFF2270 at 2298.809 kb on + strand, within GFF2270 at 2298.817 kb on - strand, within GFF2270 at 2298.820 kb on + strand, within GFF2270 at 2298.824 kb on - strand, within GFF2270 at 2298.824 kb on - strand, within GFF2270 at 2298.837 kb on - strand, within GFF2270 at 2298.869 kb on + strand, within GFF2270 at 2298.877 kb on - strand, within GFF2270 at 2298.877 kb on - strand, within GFF2270 at 2298.923 kb on + strand, within GFF2270 at 2299.059 kb on - strand, within GFF2270 at 2299.164 kb on + strand, within GFF2270 at 2299.164 kb on + strand, within GFF2270 at 2299.164 kb on + strand, within GFF2270 at 2299.172 kb on - strand, within GFF2270 at 2299.172 kb on - strand, within GFF2270 at 2299.212 kb on + strand, within GFF2270 at 2299.212 kb on + strand, within GFF2270 at 2299.217 kb on + strand, within GFF2270 at 2299.217 kb on + strand, within GFF2270 at 2299.217 kb on + strand, within GFF2270 at 2299.220 kb on - strand, within GFF2270 at 2299.222 kb on + strand, within GFF2270 at 2299.284 kb on - strand, within GFF2270 at 2299.330 kb on + strand, within GFF2270 at 2299.338 kb on - strand, within GFF2270 at 2299.377 kb on + strand, within GFF2270 at 2299.404 kb on + strand, within GFF2270 at 2299.414 kb on + strand, within GFF2270 at 2299.414 kb on + strand, within GFF2270 at 2299.414 kb on + strand, within GFF2270 at 2299.414 kb on + strand, within GFF2270 at 2299.422 kb on - strand, within GFF2270 at 2299.422 kb on - strand, within GFF2270 at 2299.422 kb on - strand, within GFF2270 at 2299.456 kb on + strand, within GFF2270 at 2299.460 kb on + strand, within GFF2270 at 2299.460 kb on + strand, within GFF2270 at 2299.460 kb on + strand, within GFF2270 at 2299.464 kb on - strand, within GFF2270 at 2299.468 kb on - strand, within GFF2270 at 2299.480 kb on - strand, within GFF2270 at 2299.489 kb on - strand, within GFF2270 at 2299.642 kb on + strand at 2299.644 kb on + strand at 2299.692 kb on + strand, within GFF2271 at 2299.692 kb on + strand, within GFF2271 at 2299.761 kb on + strand, within GFF2271 at 2299.761 kb on + strand, within GFF2271 at 2299.835 kb on - strand, within GFF2271 at 2299.848 kb on - strand, within GFF2271 at 2299.863 kb on + strand at 2299.900 kb on + strand at 2299.900 kb on + strand at 2299.904 kb on + strand at 2299.922 kb on + strand at 2299.953 kb on + strand at 2299.964 kb on + strand at 2300.001 kb on - strand at 2300.036 kb on - strand at 2300.039 kb on + strand at 2300.044 kb on + strand at 2300.047 kb on - strand at 2300.047 kb on - strand at 2300.052 kb on - strand at 2300.052 kb on - strand at 2300.063 kb on - strand at 2300.069 kb on + strand at 2300.069 kb on + strand at 2300.102 kb on - strand at 2300.161 kb on - strand, within GFF2272 at 2300.173 kb on + strand, within GFF2272 at 2300.178 kb on + strand, within GFF2272 at 2300.181 kb on - strand, within GFF2272 at 2300.192 kb on + strand, within GFF2272 at 2300.192 kb on + strand, within GFF2272 at 2300.194 kb on - strand, within GFF2272 at 2300.200 kb on - strand, within GFF2272 at 2300.200 kb on - strand, within GFF2272 at 2300.205 kb on - strand, within GFF2272 at 2300.239 kb on + strand, within GFF2272 at 2300.247 kb on - strand, within GFF2272 at 2300.249 kb on - strand, within GFF2272 at 2300.306 kb on + strand at 2300.319 kb on + strand at 2300.381 kb on - strand at 2300.394 kb on + strand at 2300.399 kb on + strand at 2300.409 kb on + strand at 2300.409 kb on + strand at 2300.419 kb on + strand at 2300.462 kb on + strand at 2300.462 kb on + strand at 2300.494 kb on + strand, within GFF2273 at 2300.551 kb on + strand, within GFF2273 at 2300.560 kb on + strand, within GFF2273 at 2300.578 kb on + strand, within GFF2273
Per-strain Table
Position Strand Gene LocusTag Fraction EMM_noCarbon with Sucrose and Putrescine remove 2,297,754 + +0.3 2,297,754 + +0.1 2,297,759 + +0.4 2,297,759 + -1.4 2,297,767 - +1.4 2,297,767 - -1.3 2,297,767 - -0.1 2,297,767 - -0.6 2,297,767 - -0.9 2,297,767 - -0.4 2,297,767 - -0.1 2,297,767 - -0.2 2,297,823 - +0.4 2,297,823 - -1.2 2,297,916 + GFF2269 0.18 -1.0 2,297,916 + GFF2269 0.18 -0.9 2,297,929 - GFF2269 0.20 -0.9 2,297,939 - GFF2269 0.21 -2.1 2,297,959 - GFF2269 0.23 -1.3 2,297,959 - GFF2269 0.23 -1.0 2,297,976 - GFF2269 0.25 -1.5 2,298,043 - GFF2269 0.32 -0.7 2,298,054 + GFF2269 0.34 -1.9 2,298,077 - GFF2269 0.36 -0.2 2,298,146 - GFF2269 0.44 -1.0 2,298,151 - GFF2269 0.44 -0.6 2,298,151 - GFF2269 0.44 -0.9 2,298,213 + GFF2269 0.51 -0.3 2,298,301 + GFF2269 0.61 -0.9 2,298,316 + GFF2269 0.62 +0.7 2,298,316 + GFF2269 0.62 -0.2 2,298,316 + GFF2269 0.62 -0.5 2,298,316 + GFF2269 0.62 -0.3 2,298,324 - GFF2269 0.63 +0.3 2,298,324 - GFF2269 0.63 -1.2 2,298,326 + GFF2269 0.64 -0.7 2,298,334 - GFF2269 0.64 -0.7 2,298,356 - GFF2269 0.67 -1.0 2,298,375 - GFF2269 0.69 +0.3 2,298,375 - GFF2269 0.69 -1.9 2,298,375 - GFF2269 0.69 -0.4 2,298,383 + GFF2269 0.70 +1.9 2,298,387 + GFF2269 0.70 -1.4 2,298,426 + GFF2269 0.75 -2.1 2,298,434 - GFF2269 0.75 -2.9 2,298,471 + GFF2269 0.80 -1.3 2,298,471 + GFF2269 0.80 -2.2 2,298,526 + GFF2269 0.86 -3.0 2,298,532 + GFF2269 0.86 -1.6 2,298,540 - GFF2269 0.87 -0.7 2,298,545 + GFF2269 0.88 -2.0 2,298,545 + GFF2269 0.88 -0.2 2,298,553 - GFF2269 0.89 +0.8 2,298,586 + -1.2 2,298,613 + -1.3 2,298,613 + -0.6 2,298,613 + -0.4 2,298,621 - -1.0 2,298,643 + -1.4 2,298,643 + -0.2 2,298,646 - -1.0 2,298,651 - -1.5 2,298,651 - +0.6 2,298,688 - -2.6 2,298,688 - -1.2 2,298,726 + -1.8 2,298,726 + -0.6 2,298,734 - +0.4 2,298,734 - -2.1 2,298,750 - GFF2270 0.10 -2.1 2,298,764 + GFF2270 0.12 -2.1 2,298,764 + GFF2270 0.12 -1.8 2,298,809 + GFF2270 0.17 -0.2 2,298,817 - GFF2270 0.17 -2.4 2,298,820 + GFF2270 0.18 -3.4 2,298,824 - GFF2270 0.18 -1.4 2,298,824 - GFF2270 0.18 -2.0 2,298,837 - GFF2270 0.19 +0.7 2,298,869 + GFF2270 0.23 -1.2 2,298,877 - GFF2270 0.24 -1.8 2,298,877 - GFF2270 0.24 -1.4 2,298,923 + GFF2270 0.29 -1.3 2,299,059 - GFF2270 0.43 -3.6 2,299,164 + GFF2270 0.54 -2.0 2,299,164 + GFF2270 0.54 -2.9 2,299,164 + GFF2270 0.54 -2.1 2,299,172 - GFF2270 0.55 -1.0 2,299,172 - GFF2270 0.55 -1.5 2,299,212 + GFF2270 0.59 -1.9 2,299,212 + GFF2270 0.59 -1.0 2,299,217 + GFF2270 0.60 -1.4 2,299,217 + GFF2270 0.60 -1.0 2,299,217 + GFF2270 0.60 -0.7 2,299,220 - GFF2270 0.60 -2.4 2,299,222 + GFF2270 0.60 -1.6 2,299,284 - GFF2270 0.67 -1.0 2,299,330 + GFF2270 0.72 -3.2 2,299,338 - GFF2270 0.72 -1.2 2,299,377 + GFF2270 0.77 -1.5 2,299,404 + GFF2270 0.79 -2.0 2,299,414 + GFF2270 0.81 -2.0 2,299,414 + GFF2270 0.81 -1.3 2,299,414 + GFF2270 0.81 -2.1 2,299,414 + GFF2270 0.81 -0.9 2,299,422 - GFF2270 0.81 -2.7 2,299,422 - GFF2270 0.81 -2.1 2,299,422 - GFF2270 0.81 -0.6 2,299,456 + GFF2270 0.85 -2.7 2,299,460 + GFF2270 0.85 -3.5 2,299,460 + GFF2270 0.85 -2.0 2,299,460 + GFF2270 0.85 +0.1 2,299,464 - GFF2270 0.86 -1.8 2,299,468 - GFF2270 0.86 -1.6 2,299,480 - GFF2270 0.88 -1.6 2,299,489 - GFF2270 0.88 -2.4 2,299,642 + +0.3 2,299,644 + +0.2 2,299,692 + GFF2271 0.11 -0.5 2,299,692 + GFF2271 0.11 +0.3 2,299,761 + GFF2271 0.44 -0.0 2,299,761 + GFF2271 0.44 -2.1 2,299,835 - GFF2271 0.79 +1.0 2,299,848 - GFF2271 0.85 -0.3 2,299,863 + -0.3 2,299,900 + -0.4 2,299,900 + +1.9 2,299,904 + +0.2 2,299,922 + +0.0 2,299,953 + -1.5 2,299,964 + +0.6 2,300,001 - +0.1 2,300,036 - +0.4 2,300,039 + -0.6 2,300,044 + +0.4 2,300,047 - -1.0 2,300,047 - +0.6 2,300,052 - -0.4 2,300,052 - +0.7 2,300,063 - -0.3 2,300,069 + -1.3 2,300,069 + +0.1 2,300,102 - -0.1 2,300,161 - GFF2272 0.34 +0.1 2,300,173 + GFF2272 0.41 -1.0 2,300,178 + GFF2272 0.44 +1.1 2,300,181 - GFF2272 0.46 -0.1 2,300,192 + GFF2272 0.52 +0.2 2,300,192 + GFF2272 0.52 +0.2 2,300,194 - GFF2272 0.53 +0.7 2,300,200 - GFF2272 0.57 -0.8 2,300,200 - GFF2272 0.57 +0.8 2,300,205 - GFF2272 0.60 -0.1 2,300,239 + GFF2272 0.80 +0.1 2,300,247 - GFF2272 0.84 -0.3 2,300,249 - GFF2272 0.85 -1.7 2,300,306 + +0.4 2,300,319 + -0.6 2,300,381 - -1.0 2,300,394 + +0.5 2,300,399 + +0.2 2,300,409 + +0.1 2,300,409 + -0.1 2,300,419 + -0.7 2,300,462 + -1.4 2,300,462 + -0.3 2,300,494 + GFF2273 0.12 +0.8 2,300,551 + GFF2273 0.16 +0.5 2,300,560 + GFF2273 0.17 -0.0 2,300,578 + GFF2273 0.18 +0.9
Or see this region's nucleotide sequence