Experiment: EMM_noCarbon with Sucrose and Putrescine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2085 and GFF2086 are separated by 4 nucleotides GFF2086 and GFF2087 are separated by 81 nucleotides GFF2087 and GFF2088 are separated by 58 nucleotides GFF2088 and GFF2089 overlap by 4 nucleotides
GFF2085 - Rhomboid family protein, at 2,119,535 to 2,120,194
GFF2085
GFF2086 - Transcription elongation factor GreA, at 2,120,199 to 2,120,675
GFF2086
GFF2087 - Carbamoyl-phosphate synthase large chain (EC 6.3.5.5), at 2,120,757 to 2,124,092
GFF2087
GFF2088 - hypothetical protein, at 2,124,151 to 2,124,519
GFF2088
GFF2089 - Carbamoyl-phosphate synthase small chain (EC 6.3.5.5), at 2,124,516 to 2,125,694
GFF2089
Position (kb)
2120
2121
2122
2123
2124
2125 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 2119.770 kb on + strand, within GFF2085 at 2119.770 kb on + strand, within GFF2085 at 2119.770 kb on + strand, within GFF2085 at 2119.770 kb on + strand, within GFF2085 at 2119.770 kb on + strand, within GFF2085 at 2119.778 kb on - strand, within GFF2085 at 2119.778 kb on - strand, within GFF2085 at 2119.778 kb on - strand, within GFF2085 at 2119.778 kb on - strand, within GFF2085 at 2119.778 kb on - strand, within GFF2085 at 2119.778 kb on - strand, within GFF2085 at 2119.778 kb on - strand, within GFF2085 at 2119.778 kb on - strand, within GFF2085 at 2119.778 kb on - strand, within GFF2085 at 2119.780 kb on + strand, within GFF2085 at 2119.788 kb on - strand, within GFF2085 at 2119.825 kb on - strand, within GFF2085 at 2119.826 kb on - strand, within GFF2085 at 2119.869 kb on - strand, within GFF2085 at 2119.869 kb on - strand, within GFF2085 at 2119.890 kb on + strand, within GFF2085 at 2119.911 kb on + strand, within GFF2085 at 2119.921 kb on + strand, within GFF2085 at 2119.924 kb on - strand, within GFF2085 at 2119.924 kb on - strand, within GFF2085 at 2119.925 kb on + strand, within GFF2085 at 2119.927 kb on + strand, within GFF2085 at 2119.964 kb on + strand, within GFF2085 at 2119.993 kb on + strand, within GFF2085 at 2120.001 kb on - strand, within GFF2085 at 2120.009 kb on - strand, within GFF2085 at 2120.022 kb on - strand, within GFF2085 at 2120.022 kb on - strand, within GFF2085 at 2120.030 kb on - strand, within GFF2085 at 2120.061 kb on - strand, within GFF2085 at 2120.084 kb on - strand, within GFF2085 at 2120.085 kb on - strand, within GFF2085 at 2120.126 kb on - strand, within GFF2085 at 2120.145 kb on + strand at 2120.148 kb on - strand at 2120.148 kb on - strand at 2120.153 kb on - strand at 2120.153 kb on - strand at 2120.153 kb on - strand at 2120.168 kb on + strand at 2120.197 kb on + strand at 2120.197 kb on + strand at 2120.202 kb on + strand at 2120.205 kb on - strand at 2120.263 kb on - strand, within GFF2086 at 2120.631 kb on + strand at 2120.666 kb on - strand at 2120.666 kb on - strand at 2120.666 kb on - strand at 2120.740 kb on - strand at 2120.763 kb on - strand at 2120.763 kb on - strand at 2120.773 kb on + strand at 2120.776 kb on - strand at 2120.781 kb on - strand at 2120.789 kb on - strand at 2124.154 kb on + strand at 2124.157 kb on - strand at 2124.157 kb on - strand at 2124.161 kb on + strand at 2124.162 kb on - strand at 2124.165 kb on - strand at 2124.169 kb on - strand at 2124.169 kb on - strand at 2124.169 kb on - strand at 2124.169 kb on - strand at 2124.190 kb on - strand, within GFF2088 at 2124.190 kb on - strand, within GFF2088 at 2124.198 kb on - strand, within GFF2088 at 2124.230 kb on + strand, within GFF2088 at 2124.247 kb on + strand, within GFF2088 at 2124.255 kb on - strand, within GFF2088 at 2124.255 kb on - strand, within GFF2088 at 2124.255 kb on - strand, within GFF2088 at 2124.255 kb on - strand, within GFF2088 at 2124.255 kb on - strand, within GFF2088 at 2124.255 kb on - strand, within GFF2088 at 2124.309 kb on - strand, within GFF2088 at 2124.350 kb on - strand, within GFF2088 at 2124.360 kb on - strand, within GFF2088 at 2124.360 kb on - strand, within GFF2088 at 2124.360 kb on - strand, within GFF2088 at 2124.360 kb on - strand, within GFF2088 at 2124.360 kb on - strand, within GFF2088 at 2124.360 kb on - strand, within GFF2088 at 2124.380 kb on - strand, within GFF2088 at 2124.458 kb on - strand, within GFF2088 at 2124.458 kb on - strand, within GFF2088 at 2124.458 kb on - strand, within GFF2088 at 2124.472 kb on - strand, within GFF2088 at 2124.472 kb on - strand, within GFF2088 at 2124.480 kb on - strand, within GFF2088 at 2124.484 kb on - strand at 2124.515 kb on - strand at 2124.516 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction EMM_noCarbon with Sucrose and Putrescine remove 2,119,770 + GFF2085 0.36 +0.4 2,119,770 + GFF2085 0.36 -0.8 2,119,770 + GFF2085 0.36 -0.8 2,119,770 + GFF2085 0.36 -0.5 2,119,770 + GFF2085 0.36 -2.3 2,119,778 - GFF2085 0.37 -0.2 2,119,778 - GFF2085 0.37 -0.2 2,119,778 - GFF2085 0.37 -0.3 2,119,778 - GFF2085 0.37 -0.8 2,119,778 - GFF2085 0.37 -0.4 2,119,778 - GFF2085 0.37 -0.8 2,119,778 - GFF2085 0.37 +0.8 2,119,778 - GFF2085 0.37 +1.2 2,119,778 - GFF2085 0.37 +0.9 2,119,780 + GFF2085 0.37 -0.1 2,119,788 - GFF2085 0.38 +0.6 2,119,825 - GFF2085 0.44 -0.2 2,119,826 - GFF2085 0.44 +1.3 2,119,869 - GFF2085 0.51 +0.4 2,119,869 - GFF2085 0.51 +0.2 2,119,890 + GFF2085 0.54 -1.2 2,119,911 + GFF2085 0.57 +0.2 2,119,921 + GFF2085 0.58 +0.8 2,119,924 - GFF2085 0.59 -1.1 2,119,924 - GFF2085 0.59 +1.2 2,119,925 + GFF2085 0.59 -0.4 2,119,927 + GFF2085 0.59 +0.5 2,119,964 + GFF2085 0.65 -0.5 2,119,993 + GFF2085 0.69 -1.1 2,120,001 - GFF2085 0.71 -0.2 2,120,009 - GFF2085 0.72 -0.9 2,120,022 - GFF2085 0.74 -0.4 2,120,022 - GFF2085 0.74 +0.2 2,120,030 - GFF2085 0.75 -0.9 2,120,061 - GFF2085 0.80 +0.2 2,120,084 - GFF2085 0.83 -1.0 2,120,085 - GFF2085 0.83 +1.2 2,120,126 - GFF2085 0.90 +0.9 2,120,145 + -1.5 2,120,148 - -0.3 2,120,148 - -1.0 2,120,153 - +0.1 2,120,153 - +1.1 2,120,153 - -1.8 2,120,168 + +0.8 2,120,197 + +0.7 2,120,197 + +0.1 2,120,202 + -0.3 2,120,205 - +0.9 2,120,263 - GFF2086 0.13 -1.3 2,120,631 + -1.3 2,120,666 - +1.6 2,120,666 - -1.8 2,120,666 - -1.8 2,120,740 - -0.7 2,120,763 - -1.4 2,120,763 - +1.0 2,120,773 + +0.1 2,120,776 - +0.1 2,120,781 - +0.6 2,120,789 - +0.2 2,124,154 + -1.7 2,124,157 - +0.6 2,124,157 - -0.5 2,124,161 + -2.0 2,124,162 - +0.2 2,124,165 - -0.1 2,124,169 - -2.0 2,124,169 - +0.7 2,124,169 - +0.5 2,124,169 - +1.5 2,124,190 - GFF2088 0.11 +0.5 2,124,190 - GFF2088 0.11 +0.5 2,124,198 - GFF2088 0.13 +1.7 2,124,230 + GFF2088 0.21 -0.7 2,124,247 + GFF2088 0.26 +0.4 2,124,255 - GFF2088 0.28 +0.0 2,124,255 - GFF2088 0.28 +0.7 2,124,255 - GFF2088 0.28 -0.1 2,124,255 - GFF2088 0.28 +0.0 2,124,255 - GFF2088 0.28 -0.3 2,124,255 - GFF2088 0.28 +0.7 2,124,309 - GFF2088 0.43 +0.1 2,124,350 - GFF2088 0.54 -0.1 2,124,360 - GFF2088 0.57 +1.5 2,124,360 - GFF2088 0.57 -0.3 2,124,360 - GFF2088 0.57 -3.4 2,124,360 - GFF2088 0.57 -0.3 2,124,360 - GFF2088 0.57 -1.3 2,124,360 - GFF2088 0.57 -0.1 2,124,380 - GFF2088 0.62 -1.8 2,124,458 - GFF2088 0.83 +0.8 2,124,458 - GFF2088 0.83 -1.1 2,124,458 - GFF2088 0.83 -0.4 2,124,472 - GFF2088 0.87 +0.6 2,124,472 - GFF2088 0.87 -0.7 2,124,480 - GFF2088 0.89 +0.1 2,124,484 - -1.7 2,124,515 - -0.0 2,124,516 - -0.6
Or see this region's nucleotide sequence