Experiment: EMM_noCarbon with Sucrose and Putrescine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1898 and GFF1899 overlap by 4 nucleotides GFF1899 and GFF1900 are separated by 30 nucleotides GFF1900 and GFF1901 are separated by 201 nucleotides GFF1901 and GFF1902 are separated by 40 nucleotides GFF1902 and GFF1903 overlap by 4 nucleotides
GFF1898 - hypothetical protein, at 1,921,420 to 1,923,753
GFF1898
GFF1899 - hypothetical protein, at 1,923,750 to 1,924,247
GFF1899
GFF1900 - Low-specificity L-threonine aldolase (EC 4.1.2.48), at 1,924,278 to 1,925,324
GFF1900
GFF1901 - Pterin-4-alpha-carbinolamine dehydratase (EC 4.2.1.96), at 1,925,526 to 1,925,816
GFF1901
GFF1902 - N-acetylglutamate kinase (EC 2.7.2.8), at 1,925,857 to 1,926,264
GFF1902
GFF1903 - ABC transporter involved in cytochrome c biogenesis, ATPase component CcmA, at 1,926,261 to 1,926,878
GFF1903
Position (kb)
1924
1925
1926 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1923.318 kb on + strand, within GFF1898 at 1923.318 kb on + strand, within GFF1898 at 1923.318 kb on + strand, within GFF1898 at 1923.326 kb on - strand, within GFF1898 at 1923.326 kb on - strand, within GFF1898 at 1923.343 kb on - strand, within GFF1898 at 1923.348 kb on + strand, within GFF1898 at 1923.369 kb on + strand, within GFF1898 at 1923.382 kb on + strand, within GFF1898 at 1923.390 kb on - strand, within GFF1898 at 1923.393 kb on + strand, within GFF1898 at 1923.407 kb on - strand, within GFF1898 at 1923.559 kb on + strand at 1923.559 kb on + strand at 1923.573 kb on + strand at 1923.577 kb on - strand at 1923.577 kb on - strand at 1923.577 kb on - strand at 1923.578 kb on - strand at 1923.581 kb on - strand at 1923.622 kb on - strand at 1923.702 kb on - strand at 1923.819 kb on + strand, within GFF1899 at 1923.827 kb on - strand, within GFF1899 at 1923.827 kb on - strand, within GFF1899 at 1923.845 kb on + strand, within GFF1899 at 1923.845 kb on + strand, within GFF1899 at 1923.845 kb on + strand, within GFF1899 at 1923.845 kb on + strand, within GFF1899 at 1923.845 kb on + strand, within GFF1899 at 1923.845 kb on + strand, within GFF1899 at 1923.845 kb on + strand, within GFF1899 at 1923.845 kb on + strand, within GFF1899 at 1923.845 kb on + strand, within GFF1899 at 1923.845 kb on + strand, within GFF1899 at 1923.850 kb on + strand, within GFF1899 at 1923.850 kb on + strand, within GFF1899 at 1923.853 kb on - strand, within GFF1899 at 1923.855 kb on + strand, within GFF1899 at 1923.855 kb on + strand, within GFF1899 at 1923.887 kb on - strand, within GFF1899 at 1923.915 kb on + strand, within GFF1899 at 1923.923 kb on - strand, within GFF1899 at 1923.923 kb on - strand, within GFF1899 at 1923.938 kb on + strand, within GFF1899 at 1923.946 kb on + strand, within GFF1899 at 1923.997 kb on + strand, within GFF1899 at 1924.001 kb on + strand, within GFF1899 at 1924.005 kb on - strand, within GFF1899 at 1924.005 kb on - strand, within GFF1899 at 1924.007 kb on + strand, within GFF1899 at 1924.009 kb on - strand, within GFF1899 at 1924.014 kb on - strand, within GFF1899 at 1924.029 kb on + strand, within GFF1899 at 1924.029 kb on + strand, within GFF1899 at 1924.037 kb on - strand, within GFF1899 at 1924.085 kb on - strand, within GFF1899 at 1924.090 kb on - strand, within GFF1899 at 1924.113 kb on - strand, within GFF1899 at 1924.160 kb on - strand, within GFF1899 at 1924.166 kb on + strand, within GFF1899 at 1924.166 kb on + strand, within GFF1899 at 1924.199 kb on - strand at 1924.213 kb on + strand at 1924.221 kb on - strand at 1924.242 kb on + strand at 1924.297 kb on - strand at 1924.299 kb on + strand at 1924.311 kb on - strand at 1924.317 kb on + strand at 1924.359 kb on + strand at 1924.386 kb on + strand, within GFF1900 at 1924.425 kb on - strand, within GFF1900 at 1924.436 kb on + strand, within GFF1900 at 1924.441 kb on + strand, within GFF1900 at 1924.441 kb on + strand, within GFF1900 at 1924.441 kb on + strand, within GFF1900 at 1924.449 kb on - strand, within GFF1900 at 1924.458 kb on + strand, within GFF1900 at 1924.459 kb on - strand, within GFF1900 at 1924.473 kb on + strand, within GFF1900 at 1924.473 kb on + strand, within GFF1900 at 1924.483 kb on + strand, within GFF1900 at 1924.484 kb on + strand, within GFF1900 at 1924.491 kb on - strand, within GFF1900 at 1924.491 kb on - strand, within GFF1900 at 1924.492 kb on - strand, within GFF1900 at 1924.493 kb on + strand, within GFF1900 at 1924.574 kb on + strand, within GFF1900 at 1924.586 kb on - strand, within GFF1900 at 1924.658 kb on + strand, within GFF1900 at 1924.686 kb on + strand, within GFF1900 at 1924.686 kb on + strand, within GFF1900 at 1924.691 kb on + strand, within GFF1900 at 1924.712 kb on + strand, within GFF1900 at 1924.712 kb on + strand, within GFF1900 at 1924.712 kb on + strand, within GFF1900 at 1924.712 kb on + strand, within GFF1900 at 1924.720 kb on - strand, within GFF1900 at 1924.720 kb on - strand, within GFF1900 at 1924.720 kb on - strand, within GFF1900 at 1924.720 kb on - strand, within GFF1900 at 1924.720 kb on - strand, within GFF1900 at 1924.720 kb on - strand, within GFF1900 at 1924.727 kb on + strand, within GFF1900 at 1924.730 kb on - strand, within GFF1900 at 1924.739 kb on + strand, within GFF1900 at 1924.744 kb on + strand, within GFF1900 at 1924.752 kb on - strand, within GFF1900 at 1924.762 kb on + strand, within GFF1900 at 1924.775 kb on - strand, within GFF1900 at 1924.775 kb on - strand, within GFF1900 at 1924.775 kb on - strand, within GFF1900 at 1924.782 kb on + strand, within GFF1900 at 1924.782 kb on + strand, within GFF1900 at 1924.782 kb on + strand, within GFF1900 at 1924.782 kb on + strand, within GFF1900 at 1924.782 kb on + strand, within GFF1900 at 1924.782 kb on + strand, within GFF1900 at 1924.788 kb on + strand, within GFF1900 at 1924.790 kb on - strand, within GFF1900 at 1924.790 kb on - strand, within GFF1900 at 1924.790 kb on - strand, within GFF1900 at 1924.790 kb on - strand, within GFF1900 at 1924.790 kb on - strand, within GFF1900 at 1924.864 kb on + strand, within GFF1900 at 1924.865 kb on - strand, within GFF1900 at 1924.865 kb on - strand, within GFF1900 at 1924.866 kb on + strand, within GFF1900 at 1924.875 kb on - strand, within GFF1900 at 1924.875 kb on - strand, within GFF1900 at 1924.875 kb on - strand, within GFF1900 at 1924.875 kb on - strand, within GFF1900 at 1924.877 kb on + strand, within GFF1900 at 1924.877 kb on + strand, within GFF1900 at 1924.879 kb on - strand, within GFF1900 at 1924.895 kb on + strand, within GFF1900 at 1924.903 kb on - strand, within GFF1900 at 1924.921 kb on + strand, within GFF1900 at 1925.006 kb on + strand, within GFF1900 at 1925.014 kb on - strand, within GFF1900 at 1925.014 kb on - strand, within GFF1900 at 1925.014 kb on - strand, within GFF1900 at 1925.055 kb on + strand, within GFF1900 at 1925.058 kb on - strand, within GFF1900 at 1925.062 kb on - strand, within GFF1900 at 1925.084 kb on - strand, within GFF1900 at 1925.092 kb on + strand, within GFF1900 at 1925.092 kb on + strand, within GFF1900 at 1925.100 kb on - strand, within GFF1900 at 1925.100 kb on - strand, within GFF1900 at 1925.153 kb on + strand, within GFF1900 at 1925.166 kb on + strand, within GFF1900 at 1925.174 kb on + strand, within GFF1900 at 1925.199 kb on - strand, within GFF1900 at 1925.201 kb on + strand, within GFF1900 at 1925.267 kb on + strand at 1925.267 kb on + strand at 1925.267 kb on + strand at 1925.267 kb on + strand at 1925.275 kb on - strand at 1925.275 kb on - strand at 1925.275 kb on - strand at 1925.275 kb on - strand at 1925.275 kb on - strand at 1925.280 kb on - strand at 1925.309 kb on + strand at 1925.311 kb on + strand at 1925.311 kb on + strand at 1925.346 kb on - strand at 1925.348 kb on - strand at 1925.353 kb on - strand at 1925.357 kb on + strand at 1925.357 kb on + strand at 1925.367 kb on + strand at 1925.371 kb on + strand at 1925.377 kb on + strand at 1925.430 kb on + strand at 1925.460 kb on - strand at 1925.476 kb on + strand at 1925.537 kb on - strand at 1925.537 kb on - strand at 1925.540 kb on + strand at 1925.542 kb on + strand at 1925.548 kb on - strand at 1925.548 kb on - strand at 1925.548 kb on - strand at 1925.548 kb on - strand at 1925.563 kb on + strand, within GFF1901 at 1925.596 kb on - strand, within GFF1901 at 1925.635 kb on - strand, within GFF1901 at 1925.705 kb on + strand, within GFF1901 at 1925.714 kb on + strand, within GFF1901 at 1925.714 kb on + strand, within GFF1901 at 1925.716 kb on + strand, within GFF1901 at 1925.726 kb on + strand, within GFF1901 at 1925.726 kb on + strand, within GFF1901 at 1925.726 kb on + strand, within GFF1901 at 1925.726 kb on + strand, within GFF1901 at 1925.731 kb on + strand, within GFF1901 at 1925.739 kb on - strand, within GFF1901 at 1925.745 kb on + strand, within GFF1901 at 1925.813 kb on + strand at 1925.826 kb on - strand at 1925.826 kb on - strand at 1925.826 kb on - strand at 1925.855 kb on - strand at 1925.859 kb on + strand at 1925.859 kb on + strand at 1925.867 kb on - strand at 1925.867 kb on - strand at 1925.972 kb on - strand, within GFF1902 at 1925.972 kb on - strand, within GFF1902 at 1925.982 kb on + strand, within GFF1902 at 1926.002 kb on + strand, within GFF1902 at 1926.130 kb on + strand, within GFF1902 at 1926.176 kb on + strand, within GFF1902 at 1926.246 kb on + strand at 1926.254 kb on - strand at 1926.254 kb on - strand at 1926.263 kb on + strand at 1926.273 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction EMM_noCarbon with Sucrose and Putrescine remove 1,923,318 + GFF1898 0.81 +0.1 1,923,318 + GFF1898 0.81 +1.2 1,923,318 + GFF1898 0.81 +0.1 1,923,326 - GFF1898 0.82 +0.6 1,923,326 - GFF1898 0.82 +2.1 1,923,343 - GFF1898 0.82 -0.5 1,923,348 + GFF1898 0.83 +0.2 1,923,369 + GFF1898 0.84 +0.3 1,923,382 + GFF1898 0.84 +0.6 1,923,390 - GFF1898 0.84 -0.5 1,923,393 + GFF1898 0.85 +0.1 1,923,407 - GFF1898 0.85 +0.5 1,923,559 + +0.3 1,923,559 + -0.0 1,923,573 + -0.4 1,923,577 - -0.4 1,923,577 - -0.1 1,923,577 - -0.9 1,923,578 - +0.7 1,923,581 - -0.4 1,923,622 - -1.0 1,923,702 - +0.2 1,923,819 + GFF1899 0.14 +1.3 1,923,827 - GFF1899 0.15 -0.9 1,923,827 - GFF1899 0.15 -0.6 1,923,845 + GFF1899 0.19 -0.2 1,923,845 + GFF1899 0.19 +0.5 1,923,845 + GFF1899 0.19 -0.4 1,923,845 + GFF1899 0.19 -2.1 1,923,845 + GFF1899 0.19 +0.5 1,923,845 + GFF1899 0.19 +0.8 1,923,845 + GFF1899 0.19 -1.0 1,923,845 + GFF1899 0.19 +0.6 1,923,845 + GFF1899 0.19 -0.1 1,923,845 + GFF1899 0.19 -1.1 1,923,850 + GFF1899 0.20 -0.4 1,923,850 + GFF1899 0.20 -1.2 1,923,853 - GFF1899 0.21 +0.4 1,923,855 + GFF1899 0.21 +0.5 1,923,855 + GFF1899 0.21 -0.2 1,923,887 - GFF1899 0.28 -0.4 1,923,915 + GFF1899 0.33 +0.6 1,923,923 - GFF1899 0.35 +0.0 1,923,923 - GFF1899 0.35 +0.8 1,923,938 + GFF1899 0.38 -2.2 1,923,946 + GFF1899 0.39 -1.3 1,923,997 + GFF1899 0.50 -1.8 1,924,001 + GFF1899 0.50 -1.9 1,924,005 - GFF1899 0.51 +0.7 1,924,005 - GFF1899 0.51 -0.2 1,924,007 + GFF1899 0.52 +0.1 1,924,009 - GFF1899 0.52 +0.3 1,924,014 - GFF1899 0.53 -2.9 1,924,029 + GFF1899 0.56 +0.3 1,924,029 + GFF1899 0.56 +0.1 1,924,037 - GFF1899 0.58 +0.5 1,924,085 - GFF1899 0.67 +0.3 1,924,090 - GFF1899 0.68 -0.0 1,924,113 - GFF1899 0.73 -0.6 1,924,160 - GFF1899 0.82 +0.4 1,924,166 + GFF1899 0.84 +1.2 1,924,166 + GFF1899 0.84 -0.1 1,924,199 - +0.6 1,924,213 + +0.2 1,924,221 - +1.4 1,924,242 + +0.1 1,924,297 - +0.0 1,924,299 + +0.5 1,924,311 - -0.8 1,924,317 + +1.0 1,924,359 + +0.1 1,924,386 + GFF1900 0.10 -0.0 1,924,425 - GFF1900 0.14 -1.6 1,924,436 + GFF1900 0.15 -1.3 1,924,441 + GFF1900 0.16 -0.1 1,924,441 + GFF1900 0.16 -0.7 1,924,441 + GFF1900 0.16 +0.8 1,924,449 - GFF1900 0.16 +0.7 1,924,458 + GFF1900 0.17 +1.9 1,924,459 - GFF1900 0.17 +0.0 1,924,473 + GFF1900 0.19 -1.9 1,924,473 + GFF1900 0.19 -0.6 1,924,483 + GFF1900 0.20 -1.2 1,924,484 + GFF1900 0.20 -0.7 1,924,491 - GFF1900 0.20 +2.0 1,924,491 - GFF1900 0.20 -1.8 1,924,492 - GFF1900 0.20 -1.1 1,924,493 + GFF1900 0.21 +0.6 1,924,574 + GFF1900 0.28 +0.0 1,924,586 - GFF1900 0.29 +0.3 1,924,658 + GFF1900 0.36 +0.0 1,924,686 + GFF1900 0.39 -0.9 1,924,686 + GFF1900 0.39 -0.5 1,924,691 + GFF1900 0.39 -1.7 1,924,712 + GFF1900 0.41 +0.1 1,924,712 + GFF1900 0.41 +1.7 1,924,712 + GFF1900 0.41 -1.4 1,924,712 + GFF1900 0.41 +0.8 1,924,720 - GFF1900 0.42 +1.1 1,924,720 - GFF1900 0.42 +1.0 1,924,720 - GFF1900 0.42 +1.0 1,924,720 - GFF1900 0.42 +0.4 1,924,720 - GFF1900 0.42 -1.6 1,924,720 - GFF1900 0.42 +0.7 1,924,727 + GFF1900 0.43 -0.0 1,924,730 - GFF1900 0.43 -0.2 1,924,739 + GFF1900 0.44 +0.3 1,924,744 + GFF1900 0.45 -0.4 1,924,752 - GFF1900 0.45 +0.8 1,924,762 + GFF1900 0.46 -0.3 1,924,775 - GFF1900 0.47 -0.9 1,924,775 - GFF1900 0.47 -0.8 1,924,775 - GFF1900 0.47 -0.2 1,924,782 + GFF1900 0.48 -0.2 1,924,782 + GFF1900 0.48 -0.4 1,924,782 + GFF1900 0.48 +0.1 1,924,782 + GFF1900 0.48 +0.7 1,924,782 + GFF1900 0.48 +0.7 1,924,782 + GFF1900 0.48 -1.5 1,924,788 + GFF1900 0.49 -0.5 1,924,790 - GFF1900 0.49 -0.2 1,924,790 - GFF1900 0.49 -2.0 1,924,790 - GFF1900 0.49 -0.5 1,924,790 - GFF1900 0.49 +0.8 1,924,790 - GFF1900 0.49 -0.2 1,924,864 + GFF1900 0.56 +1.4 1,924,865 - GFF1900 0.56 +0.4 1,924,865 - GFF1900 0.56 +0.7 1,924,866 + GFF1900 0.56 -0.2 1,924,875 - GFF1900 0.57 -0.4 1,924,875 - GFF1900 0.57 -0.9 1,924,875 - GFF1900 0.57 -2.0 1,924,875 - GFF1900 0.57 -1.4 1,924,877 + GFF1900 0.57 -1.0 1,924,877 + GFF1900 0.57 +0.2 1,924,879 - GFF1900 0.57 +0.8 1,924,895 + GFF1900 0.59 +0.9 1,924,903 - GFF1900 0.60 -0.5 1,924,921 + GFF1900 0.61 -0.6 1,925,006 + GFF1900 0.70 +0.3 1,925,014 - GFF1900 0.70 +0.8 1,925,014 - GFF1900 0.70 +1.0 1,925,014 - GFF1900 0.70 -1.6 1,925,055 + GFF1900 0.74 +0.6 1,925,058 - GFF1900 0.74 -1.1 1,925,062 - GFF1900 0.75 -0.3 1,925,084 - GFF1900 0.77 +0.5 1,925,092 + GFF1900 0.78 -0.7 1,925,092 + GFF1900 0.78 -1.4 1,925,100 - GFF1900 0.79 +0.9 1,925,100 - GFF1900 0.79 -0.3 1,925,153 + GFF1900 0.84 +0.4 1,925,166 + GFF1900 0.85 -1.8 1,925,174 + GFF1900 0.86 +0.5 1,925,199 - GFF1900 0.88 -0.5 1,925,201 + GFF1900 0.88 +0.7 1,925,267 + +0.6 1,925,267 + +0.3 1,925,267 + +0.2 1,925,267 + -0.8 1,925,275 - -0.7 1,925,275 - +0.6 1,925,275 - -0.1 1,925,275 - -1.0 1,925,275 - +0.8 1,925,280 - +0.0 1,925,309 + -0.6 1,925,311 + +0.3 1,925,311 + +0.1 1,925,346 - -0.2 1,925,348 - +0.6 1,925,353 - +1.0 1,925,357 + -0.6 1,925,357 + +0.6 1,925,367 + +1.2 1,925,371 + +1.2 1,925,377 + -0.8 1,925,430 + -0.3 1,925,460 - +0.2 1,925,476 + -0.1 1,925,537 - +0.2 1,925,537 - -1.2 1,925,540 + -2.7 1,925,542 + -0.6 1,925,548 - -0.0 1,925,548 - +1.0 1,925,548 - +1.4 1,925,548 - +0.1 1,925,563 + GFF1901 0.13 -0.8 1,925,596 - GFF1901 0.24 -0.6 1,925,635 - GFF1901 0.37 -0.3 1,925,705 + GFF1901 0.62 -0.4 1,925,714 + GFF1901 0.65 -1.9 1,925,714 + GFF1901 0.65 +0.7 1,925,716 + GFF1901 0.65 +0.5 1,925,726 + GFF1901 0.69 +0.5 1,925,726 + GFF1901 0.69 -2.3 1,925,726 + GFF1901 0.69 +0.5 1,925,726 + GFF1901 0.69 -1.8 1,925,731 + GFF1901 0.70 -0.3 1,925,739 - GFF1901 0.73 -0.5 1,925,745 + GFF1901 0.75 -0.2 1,925,813 + +1.2 1,925,826 - -0.6 1,925,826 - +0.0 1,925,826 - -0.5 1,925,855 - +1.0 1,925,859 + -0.1 1,925,859 + +0.6 1,925,867 - -1.7 1,925,867 - +0.7 1,925,972 - GFF1902 0.28 -1.0 1,925,972 - GFF1902 0.28 -0.1 1,925,982 + GFF1902 0.31 -0.5 1,926,002 + GFF1902 0.36 -0.8 1,926,130 + GFF1902 0.67 -2.6 1,926,176 + GFF1902 0.78 +1.3 1,926,246 + -0.1 1,926,254 - -1.5 1,926,254 - -0.3 1,926,263 + +1.0 1,926,273 + -0.2
Or see this region's nucleotide sequence