Experiment: EMM_noCarbon with Sucrose and Putrescine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1856 and GFF1857 overlap by 40 nucleotides GFF1857 and GFF1858 overlap by 4 nucleotides GFF1858 and GFF1859 overlap by 4 nucleotides GFF1859 and GFF1860 overlap by 1 nucleotides
GFF1856 - Precorrin-6A reductase (EC 1.3.1.54), at 1,886,222 to 1,886,995
GFF1856
GFF1857 - Precorrin-3B C(17)-methyltransferase (EC 2.1.1.131), at 1,886,956 to 1,887,738
GFF1857
GFF1858 - Precorrin-2 C(20)-methyltransferase (EC 2.1.1.130), at 1,887,735 to 1,888,457
GFF1858
GFF1859 - Precorrin-8X methylmutase (EC 5.4.99.61), at 1,888,454 to 1,889,083
GFF1859
GFF1860 - Precorrin-3B synthase, at 1,889,083 to 1,890,393
GFF1860
Position (kb)
1887
1888
1889 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1886.735 kb on - strand, within GFF1856 at 1886.735 kb on - strand, within GFF1856 at 1886.772 kb on + strand, within GFF1856 at 1886.772 kb on + strand, within GFF1856 at 1886.772 kb on + strand, within GFF1856 at 1886.777 kb on + strand, within GFF1856 at 1886.780 kb on - strand, within GFF1856 at 1886.780 kb on - strand, within GFF1856 at 1886.780 kb on - strand, within GFF1856 at 1886.780 kb on - strand, within GFF1856 at 1886.780 kb on - strand, within GFF1856 at 1886.780 kb on - strand, within GFF1856 at 1886.848 kb on + strand, within GFF1856 at 1886.848 kb on + strand, within GFF1856 at 1886.856 kb on - strand, within GFF1856 at 1886.865 kb on + strand, within GFF1856 at 1886.865 kb on + strand, within GFF1856 at 1886.865 kb on + strand, within GFF1856 at 1886.865 kb on + strand, within GFF1856 at 1886.865 kb on + strand, within GFF1856 at 1886.869 kb on + strand, within GFF1856 at 1886.873 kb on - strand, within GFF1856 at 1886.873 kb on - strand, within GFF1856 at 1886.873 kb on - strand, within GFF1856 at 1886.877 kb on - strand, within GFF1856 at 1886.912 kb on + strand, within GFF1856 at 1886.918 kb on + strand at 1886.929 kb on + strand at 1886.931 kb on + strand at 1886.939 kb on + strand at 1886.947 kb on - strand at 1886.947 kb on - strand at 1886.965 kb on - strand at 1886.970 kb on + strand at 1886.990 kb on + strand at 1886.996 kb on - strand at 1886.996 kb on - strand at 1886.997 kb on + strand at 1886.997 kb on + strand at 1886.997 kb on + strand at 1886.998 kb on - strand at 1887.000 kb on - strand at 1887.011 kb on - strand at 1887.028 kb on + strand at 1887.036 kb on - strand, within GFF1857 at 1887.080 kb on - strand, within GFF1857 at 1887.090 kb on - strand, within GFF1857 at 1887.136 kb on + strand, within GFF1857 at 1887.201 kb on - strand, within GFF1857 at 1887.237 kb on - strand, within GFF1857 at 1887.445 kb on - strand, within GFF1857 at 1887.445 kb on - strand, within GFF1857 at 1887.445 kb on - strand, within GFF1857 at 1887.455 kb on - strand, within GFF1857 at 1887.498 kb on + strand, within GFF1857 at 1887.536 kb on + strand, within GFF1857 at 1887.541 kb on + strand, within GFF1857 at 1887.541 kb on + strand, within GFF1857 at 1887.541 kb on + strand, within GFF1857 at 1887.541 kb on + strand, within GFF1857 at 1887.546 kb on + strand, within GFF1857 at 1887.549 kb on - strand, within GFF1857 at 1887.589 kb on - strand, within GFF1857 at 1887.605 kb on + strand, within GFF1857 at 1887.613 kb on - strand, within GFF1857 at 1887.620 kb on - strand, within GFF1857 at 1887.639 kb on - strand, within GFF1857 at 1887.645 kb on + strand, within GFF1857 at 1887.658 kb on - strand, within GFF1857 at 1887.658 kb on - strand, within GFF1857 at 1887.667 kb on + strand at 1887.724 kb on - strand at 1887.733 kb on + strand at 1887.735 kb on - strand at 1887.754 kb on + strand at 1887.790 kb on - strand at 1887.821 kb on + strand, within GFF1858 at 1887.886 kb on - strand, within GFF1858 at 1887.965 kb on - strand, within GFF1858 at 1887.982 kb on - strand, within GFF1858 at 1888.031 kb on + strand, within GFF1858 at 1888.076 kb on - strand, within GFF1858 at 1888.109 kb on - strand, within GFF1858 at 1888.138 kb on + strand, within GFF1858 at 1888.146 kb on + strand, within GFF1858 at 1888.146 kb on + strand, within GFF1858 at 1888.212 kb on - strand, within GFF1858 at 1888.311 kb on - strand, within GFF1858 at 1888.316 kb on - strand, within GFF1858 at 1888.418 kb on - strand at 1888.430 kb on + strand at 1888.447 kb on + strand at 1888.511 kb on - strand at 1888.532 kb on + strand, within GFF1859 at 1888.544 kb on - strand, within GFF1859 at 1888.551 kb on + strand, within GFF1859 at 1888.571 kb on - strand, within GFF1859 at 1888.628 kb on + strand, within GFF1859 at 1888.654 kb on + strand, within GFF1859 at 1888.661 kb on - strand, within GFF1859 at 1888.749 kb on + strand, within GFF1859 at 1888.749 kb on - strand, within GFF1859 at 1888.751 kb on - strand, within GFF1859 at 1888.757 kb on - strand, within GFF1859 at 1888.776 kb on + strand, within GFF1859 at 1888.781 kb on + strand, within GFF1859 at 1888.782 kb on - strand, within GFF1859 at 1888.791 kb on + strand, within GFF1859 at 1888.791 kb on + strand, within GFF1859 at 1888.799 kb on - strand, within GFF1859 at 1888.799 kb on - strand, within GFF1859 at 1888.799 kb on - strand, within GFF1859 at 1888.799 kb on - strand, within GFF1859 at 1888.822 kb on - strand, within GFF1859 at 1888.827 kb on + strand, within GFF1859 at 1888.827 kb on + strand, within GFF1859 at 1888.838 kb on + strand, within GFF1859 at 1888.857 kb on + strand, within GFF1859 at 1888.888 kb on - strand, within GFF1859 at 1889.089 kb on - strand at 1889.090 kb on + strand at 1889.119 kb on + strand at 1889.124 kb on + strand at 1889.124 kb on + strand at 1889.124 kb on + strand at 1889.124 kb on + strand at 1889.124 kb on + strand at 1889.124 kb on + strand at 1889.129 kb on + strand at 1889.129 kb on + strand at 1889.129 kb on + strand at 1889.132 kb on - strand at 1889.132 kb on - strand at 1889.132 kb on - strand at 1889.132 kb on - strand at 1889.132 kb on - strand at 1889.137 kb on - strand at 1889.148 kb on - strand at 1889.163 kb on - strand at 1889.178 kb on - strand at 1889.199 kb on - strand at 1889.230 kb on - strand, within GFF1860 at 1889.235 kb on - strand, within GFF1860 at 1889.235 kb on - strand, within GFF1860 at 1889.237 kb on - strand, within GFF1860 at 1889.247 kb on + strand, within GFF1860 at 1889.255 kb on - strand, within GFF1860 at 1889.298 kb on + strand, within GFF1860 at 1889.311 kb on - strand, within GFF1860 at 1889.338 kb on + strand, within GFF1860
Per-strain Table
Position Strand Gene LocusTag Fraction EMM_noCarbon with Sucrose and Putrescine remove 1,886,735 - GFF1856 0.66 -0.2 1,886,735 - GFF1856 0.66 +0.6 1,886,772 + GFF1856 0.71 +0.1 1,886,772 + GFF1856 0.71 +0.4 1,886,772 + GFF1856 0.71 -0.1 1,886,777 + GFF1856 0.72 -0.3 1,886,780 - GFF1856 0.72 +1.1 1,886,780 - GFF1856 0.72 +1.9 1,886,780 - GFF1856 0.72 -0.7 1,886,780 - GFF1856 0.72 -0.3 1,886,780 - GFF1856 0.72 +0.1 1,886,780 - GFF1856 0.72 -0.5 1,886,848 + GFF1856 0.81 +0.1 1,886,848 + GFF1856 0.81 -1.0 1,886,856 - GFF1856 0.82 +0.6 1,886,865 + GFF1856 0.83 -0.1 1,886,865 + GFF1856 0.83 -1.3 1,886,865 + GFF1856 0.83 +0.5 1,886,865 + GFF1856 0.83 +0.5 1,886,865 + GFF1856 0.83 +0.3 1,886,869 + GFF1856 0.84 -1.9 1,886,873 - GFF1856 0.84 +0.2 1,886,873 - GFF1856 0.84 +1.0 1,886,873 - GFF1856 0.84 -1.1 1,886,877 - GFF1856 0.85 -0.5 1,886,912 + GFF1856 0.89 -0.7 1,886,918 + -0.7 1,886,929 + -0.5 1,886,931 + -1.4 1,886,939 + -1.3 1,886,947 - -1.0 1,886,947 - -0.4 1,886,965 - +0.8 1,886,970 + +0.3 1,886,990 + -0.0 1,886,996 - +1.0 1,886,996 - -1.5 1,886,997 + -0.7 1,886,997 + -1.6 1,886,997 + +1.0 1,886,998 - -0.6 1,887,000 - +1.4 1,887,011 - +0.1 1,887,028 + -0.0 1,887,036 - GFF1857 0.10 +0.1 1,887,080 - GFF1857 0.16 -1.7 1,887,090 - GFF1857 0.17 -0.8 1,887,136 + GFF1857 0.23 -0.6 1,887,201 - GFF1857 0.31 +0.5 1,887,237 - GFF1857 0.36 -1.6 1,887,445 - GFF1857 0.62 +0.3 1,887,445 - GFF1857 0.62 -1.0 1,887,445 - GFF1857 0.62 +1.0 1,887,455 - GFF1857 0.64 -2.7 1,887,498 + GFF1857 0.69 +0.3 1,887,536 + GFF1857 0.74 -0.2 1,887,541 + GFF1857 0.75 -1.5 1,887,541 + GFF1857 0.75 -1.0 1,887,541 + GFF1857 0.75 -1.0 1,887,541 + GFF1857 0.75 +2.0 1,887,546 + GFF1857 0.75 -1.3 1,887,549 - GFF1857 0.76 -0.6 1,887,589 - GFF1857 0.81 +1.4 1,887,605 + GFF1857 0.83 +1.3 1,887,613 - GFF1857 0.84 +0.1 1,887,620 - GFF1857 0.85 -0.9 1,887,639 - GFF1857 0.87 -0.0 1,887,645 + GFF1857 0.88 -1.0 1,887,658 - GFF1857 0.90 -1.7 1,887,658 - GFF1857 0.90 +0.4 1,887,667 + -1.0 1,887,724 - +1.2 1,887,733 + -1.9 1,887,735 - +0.2 1,887,754 + +0.2 1,887,790 - -0.4 1,887,821 + GFF1858 0.12 -0.8 1,887,886 - GFF1858 0.21 -1.0 1,887,965 - GFF1858 0.32 -1.6 1,887,982 - GFF1858 0.34 -1.4 1,888,031 + GFF1858 0.41 -1.6 1,888,076 - GFF1858 0.47 -1.2 1,888,109 - GFF1858 0.52 -0.3 1,888,138 + GFF1858 0.56 -1.3 1,888,146 + GFF1858 0.57 -1.3 1,888,146 + GFF1858 0.57 -1.1 1,888,212 - GFF1858 0.66 -0.8 1,888,311 - GFF1858 0.80 -0.9 1,888,316 - GFF1858 0.80 +0.9 1,888,418 - -0.9 1,888,430 + -1.7 1,888,447 + -1.7 1,888,511 - -0.7 1,888,532 + GFF1859 0.12 -0.9 1,888,544 - GFF1859 0.14 +1.3 1,888,551 + GFF1859 0.15 -1.0 1,888,571 - GFF1859 0.19 -0.8 1,888,628 + GFF1859 0.28 +0.2 1,888,654 + GFF1859 0.32 +1.7 1,888,661 - GFF1859 0.33 -0.3 1,888,749 + GFF1859 0.47 -0.8 1,888,749 - GFF1859 0.47 +1.5 1,888,751 - GFF1859 0.47 -0.2 1,888,757 - GFF1859 0.48 -0.2 1,888,776 + GFF1859 0.51 +0.1 1,888,781 + GFF1859 0.52 -0.0 1,888,782 - GFF1859 0.52 -0.9 1,888,791 + GFF1859 0.53 -0.2 1,888,791 + GFF1859 0.53 -0.3 1,888,799 - GFF1859 0.55 -0.5 1,888,799 - GFF1859 0.55 +0.6 1,888,799 - GFF1859 0.55 -0.9 1,888,799 - GFF1859 0.55 +1.6 1,888,822 - GFF1859 0.58 +0.3 1,888,827 + GFF1859 0.59 +0.3 1,888,827 + GFF1859 0.59 +1.2 1,888,838 + GFF1859 0.61 +0.3 1,888,857 + GFF1859 0.64 -0.8 1,888,888 - GFF1859 0.69 +0.3 1,889,089 - -0.0 1,889,090 + -0.9 1,889,119 + -2.4 1,889,124 + -1.3 1,889,124 + -2.3 1,889,124 + -1.2 1,889,124 + +0.4 1,889,124 + -1.0 1,889,124 + -0.9 1,889,129 + -0.0 1,889,129 + -0.5 1,889,129 + -0.0 1,889,132 - +0.6 1,889,132 - +1.5 1,889,132 - +0.8 1,889,132 - +0.4 1,889,132 - -0.9 1,889,137 - +2.0 1,889,148 - +0.3 1,889,163 - -0.3 1,889,178 - -0.3 1,889,199 - -0.6 1,889,230 - GFF1860 0.11 +1.3 1,889,235 - GFF1860 0.12 +1.3 1,889,235 - GFF1860 0.12 -0.0 1,889,237 - GFF1860 0.12 -0.0 1,889,247 + GFF1860 0.13 -0.5 1,889,255 - GFF1860 0.13 -1.0 1,889,298 + GFF1860 0.16 -2.5 1,889,311 - GFF1860 0.17 -0.5 1,889,338 + GFF1860 0.19 -0.7
Or see this region's nucleotide sequence