Experiment: EMM_noCarbon with Sucrose and Putrescine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1624 and GFF1625 are separated by 38 nucleotides GFF1625 and GFF1626 are separated by 96 nucleotides GFF1626 and GFF1627 are separated by 2 nucleotides GFF1627 and GFF1628 overlap by 4 nucleotides
GFF1624 - Lipid A biosynthesis lauroyl acyltransferase (EC 2.3.1.241), at 1,652,895 to 1,653,746
GFF1624
GFF1625 - Phosphoribosylanthranilate isomerase (EC 5.3.1.24), at 1,653,785 to 1,654,429
GFF1625
GFF1626 - Tryptophan synthase beta chain (EC 4.2.1.20), at 1,654,526 to 1,655,779
GFF1626
GFF1627 - Tryptophan synthase alpha chain (EC 4.2.1.20), at 1,655,782 to 1,656,600
GFF1627
GFF1628 - Acetyl-coenzyme A carboxyl transferase beta chain (EC 6.4.1.2), at 1,656,597 to 1,657,445
GFF1628
Position (kb)
1654
1655
1656 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1653.565 kb on + strand, within GFF1624 at 1653.602 kb on + strand, within GFF1624 at 1653.607 kb on + strand, within GFF1624 at 1653.615 kb on - strand, within GFF1624 at 1653.655 kb on - strand, within GFF1624 at 1653.665 kb on - strand at 1653.680 kb on + strand at 1653.683 kb on - strand at 1653.688 kb on - strand at 1653.688 kb on - strand at 1653.693 kb on - strand at 1653.693 kb on - strand at 1653.702 kb on - strand at 1653.706 kb on + strand at 1653.719 kb on - strand at 1653.721 kb on + strand at 1653.721 kb on + strand at 1653.721 kb on + strand at 1653.721 kb on + strand at 1653.724 kb on - strand at 1653.729 kb on - strand at 1653.729 kb on - strand at 1653.729 kb on - strand at 1653.729 kb on - strand at 1653.729 kb on - strand at 1653.729 kb on - strand at 1653.729 kb on - strand at 1653.729 kb on - strand at 1653.729 kb on - strand at 1653.731 kb on + strand at 1653.731 kb on + strand at 1653.734 kb on - strand at 1653.734 kb on - strand at 1653.734 kb on - strand at 1653.739 kb on - strand at 1653.739 kb on - strand at 1653.741 kb on + strand at 1653.749 kb on - strand at 1653.749 kb on - strand at 1653.769 kb on + strand at 1653.769 kb on + strand at 1653.777 kb on - strand at 1653.777 kb on - strand at 1653.777 kb on - strand at 1653.777 kb on - strand at 1653.845 kb on - strand at 1653.850 kb on + strand, within GFF1625 at 1653.936 kb on - strand, within GFF1625 at 1653.946 kb on - strand, within GFF1625 at 1653.946 kb on - strand, within GFF1625 at 1653.998 kb on + strand, within GFF1625 at 1654.008 kb on + strand, within GFF1625 at 1654.050 kb on + strand, within GFF1625 at 1654.101 kb on + strand, within GFF1625 at 1654.112 kb on + strand, within GFF1625 at 1654.112 kb on + strand, within GFF1625 at 1654.112 kb on + strand, within GFF1625 at 1654.120 kb on - strand, within GFF1625 at 1654.139 kb on + strand, within GFF1625 at 1654.139 kb on + strand, within GFF1625 at 1654.139 kb on + strand, within GFF1625 at 1654.139 kb on + strand, within GFF1625 at 1654.139 kb on + strand, within GFF1625 at 1654.139 kb on + strand, within GFF1625 at 1654.139 kb on + strand, within GFF1625 at 1654.139 kb on + strand, within GFF1625 at 1654.144 kb on + strand, within GFF1625 at 1654.147 kb on - strand, within GFF1625 at 1654.147 kb on - strand, within GFF1625 at 1654.147 kb on - strand, within GFF1625 at 1654.147 kb on - strand, within GFF1625 at 1654.147 kb on - strand, within GFF1625 at 1654.147 kb on - strand, within GFF1625 at 1654.147 kb on - strand, within GFF1625 at 1654.147 kb on - strand, within GFF1625 at 1654.147 kb on - strand, within GFF1625 at 1654.147 kb on - strand, within GFF1625 at 1654.147 kb on - strand, within GFF1625 at 1654.147 kb on - strand, within GFF1625 at 1654.147 kb on - strand, within GFF1625 at 1654.147 kb on - strand, within GFF1625 at 1654.147 kb on - strand, within GFF1625 at 1654.147 kb on - strand, within GFF1625 at 1654.147 kb on - strand, within GFF1625 at 1654.147 kb on - strand, within GFF1625 at 1654.147 kb on - strand, within GFF1625 at 1654.147 kb on - strand, within GFF1625 at 1654.147 kb on - strand, within GFF1625 at 1654.147 kb on - strand, within GFF1625 at 1654.147 kb on - strand, within GFF1625 at 1654.147 kb on - strand, within GFF1625 at 1654.152 kb on - strand, within GFF1625 at 1654.152 kb on - strand, within GFF1625 at 1654.174 kb on + strand, within GFF1625 at 1654.202 kb on + strand, within GFF1625 at 1654.205 kb on - strand, within GFF1625 at 1654.210 kb on - strand, within GFF1625 at 1654.220 kb on + strand, within GFF1625 at 1654.220 kb on + strand, within GFF1625 at 1654.223 kb on - strand, within GFF1625 at 1654.228 kb on - strand, within GFF1625 at 1654.239 kb on - strand, within GFF1625 at 1654.269 kb on + strand, within GFF1625 at 1654.274 kb on + strand, within GFF1625 at 1654.300 kb on - strand, within GFF1625 at 1654.362 kb on + strand, within GFF1625 at 1654.363 kb on - strand, within GFF1625 at 1654.373 kb on + strand at 1654.375 kb on + strand at 1654.381 kb on - strand at 1654.383 kb on - strand at 1654.383 kb on - strand at 1654.383 kb on - strand at 1654.391 kb on - strand at 1654.391 kb on - strand at 1654.393 kb on + strand at 1654.393 kb on + strand at 1654.401 kb on - strand at 1654.437 kb on + strand at 1654.439 kb on + strand at 1654.444 kb on + strand at 1654.445 kb on - strand at 1654.447 kb on - strand at 1654.534 kb on + strand at 1654.675 kb on + strand, within GFF1626 at 1655.124 kb on + strand, within GFF1626 at 1655.317 kb on + strand, within GFF1626 at 1655.443 kb on + strand, within GFF1626 at 1655.452 kb on + strand, within GFF1626 at 1655.452 kb on + strand, within GFF1626 at 1655.460 kb on - strand, within GFF1626 at 1655.468 kb on + strand, within GFF1626 at 1655.488 kb on + strand, within GFF1626 at 1655.501 kb on + strand, within GFF1626 at 1655.501 kb on + strand, within GFF1626 at 1655.514 kb on - strand, within GFF1626 at 1655.523 kb on + strand, within GFF1626 at 1655.617 kb on + strand, within GFF1626 at 1655.624 kb on + strand, within GFF1626 at 1655.742 kb on + strand at 1655.778 kb on + strand at 1655.778 kb on + strand at 1655.821 kb on + strand at 1655.821 kb on + strand at 1655.826 kb on + strand at 1655.849 kb on - strand at 1655.863 kb on + strand at 1655.892 kb on + strand, within GFF1627 at 1655.991 kb on + strand, within GFF1627 at 1656.127 kb on + strand, within GFF1627 at 1656.135 kb on + strand, within GFF1627 at 1656.135 kb on + strand, within GFF1627 at 1656.135 kb on + strand, within GFF1627 at 1656.135 kb on + strand, within GFF1627 at 1656.135 kb on + strand, within GFF1627 at 1656.135 kb on + strand, within GFF1627 at 1656.135 kb on + strand, within GFF1627 at 1656.135 kb on + strand, within GFF1627 at 1656.135 kb on + strand, within GFF1627 at 1656.135 kb on + strand, within GFF1627 at 1656.135 kb on + strand, within GFF1627 at 1656.135 kb on + strand, within GFF1627 at 1656.135 kb on + strand, within GFF1627 at 1656.135 kb on + strand, within GFF1627 at 1656.135 kb on + strand, within GFF1627 at 1656.135 kb on + strand, within GFF1627 at 1656.135 kb on + strand, within GFF1627 at 1656.135 kb on + strand, within GFF1627 at 1656.135 kb on + strand, within GFF1627 at 1656.135 kb on + strand, within GFF1627 at 1656.135 kb on + strand, within GFF1627 at 1656.135 kb on + strand, within GFF1627 at 1656.135 kb on + strand, within GFF1627 at 1656.135 kb on + strand, within GFF1627 at 1656.135 kb on + strand, within GFF1627 at 1656.135 kb on + strand, within GFF1627 at 1656.135 kb on + strand, within GFF1627 at 1656.135 kb on + strand, within GFF1627 at 1656.135 kb on + strand, within GFF1627 at 1656.135 kb on + strand, within GFF1627 at 1656.135 kb on + strand, within GFF1627 at 1656.135 kb on + strand, within GFF1627 at 1656.135 kb on + strand, within GFF1627 at 1656.135 kb on + strand, within GFF1627 at 1656.135 kb on + strand, within GFF1627 at 1656.135 kb on + strand, within GFF1627 at 1656.143 kb on - strand, within GFF1627 at 1656.143 kb on - strand, within GFF1627 at 1656.157 kb on + strand, within GFF1627 at 1656.157 kb on + strand, within GFF1627 at 1656.381 kb on + strand, within GFF1627 at 1656.565 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction EMM_noCarbon with Sucrose and Putrescine remove 1,653,565 + GFF1624 0.79 -0.2 1,653,602 + GFF1624 0.83 -1.3 1,653,607 + GFF1624 0.84 -0.3 1,653,615 - GFF1624 0.85 -0.1 1,653,655 - GFF1624 0.89 -0.7 1,653,665 - -0.4 1,653,680 + -0.5 1,653,683 - -0.5 1,653,688 - -0.5 1,653,688 - +1.3 1,653,693 - +1.0 1,653,693 - +0.3 1,653,702 - +1.0 1,653,706 + -1.2 1,653,719 - -0.7 1,653,721 + -0.9 1,653,721 + +0.5 1,653,721 + -0.3 1,653,721 + +0.6 1,653,724 - +1.3 1,653,729 - -1.0 1,653,729 - -1.3 1,653,729 - -2.7 1,653,729 - -0.5 1,653,729 - +0.2 1,653,729 - +1.2 1,653,729 - +0.3 1,653,729 - +0.5 1,653,729 - +1.3 1,653,731 + -0.4 1,653,731 + -0.8 1,653,734 - -1.1 1,653,734 - -0.7 1,653,734 - -0.5 1,653,739 - +0.0 1,653,739 - -0.6 1,653,741 + +0.9 1,653,749 - +0.1 1,653,749 - +0.5 1,653,769 + +0.8 1,653,769 + -0.3 1,653,777 - +0.4 1,653,777 - +0.3 1,653,777 - -0.9 1,653,777 - +0.0 1,653,845 - -1.3 1,653,850 + GFF1625 0.10 -1.8 1,653,936 - GFF1625 0.23 -2.0 1,653,946 - GFF1625 0.25 -2.3 1,653,946 - GFF1625 0.25 -2.4 1,653,998 + GFF1625 0.33 -1.1 1,654,008 + GFF1625 0.35 -1.3 1,654,050 + GFF1625 0.41 -2.1 1,654,101 + GFF1625 0.49 -1.9 1,654,112 + GFF1625 0.51 -2.4 1,654,112 + GFF1625 0.51 -2.7 1,654,112 + GFF1625 0.51 -3.6 1,654,120 - GFF1625 0.52 -2.6 1,654,139 + GFF1625 0.55 -3.4 1,654,139 + GFF1625 0.55 -2.3 1,654,139 + GFF1625 0.55 -1.7 1,654,139 + GFF1625 0.55 -3.4 1,654,139 + GFF1625 0.55 -3.3 1,654,139 + GFF1625 0.55 -1.9 1,654,139 + GFF1625 0.55 -1.1 1,654,139 + GFF1625 0.55 -2.2 1,654,144 + GFF1625 0.56 -2.3 1,654,147 - GFF1625 0.56 -2.9 1,654,147 - GFF1625 0.56 -2.3 1,654,147 - GFF1625 0.56 -0.8 1,654,147 - GFF1625 0.56 -3.1 1,654,147 - GFF1625 0.56 -3.0 1,654,147 - GFF1625 0.56 +0.0 1,654,147 - GFF1625 0.56 -3.5 1,654,147 - GFF1625 0.56 -2.8 1,654,147 - GFF1625 0.56 -2.1 1,654,147 - GFF1625 0.56 -2.1 1,654,147 - GFF1625 0.56 -3.4 1,654,147 - GFF1625 0.56 -2.3 1,654,147 - GFF1625 0.56 -0.2 1,654,147 - GFF1625 0.56 -1.1 1,654,147 - GFF1625 0.56 +1.9 1,654,147 - GFF1625 0.56 -1.8 1,654,147 - GFF1625 0.56 -1.5 1,654,147 - GFF1625 0.56 -0.4 1,654,147 - GFF1625 0.56 -2.1 1,654,147 - GFF1625 0.56 -1.8 1,654,147 - GFF1625 0.56 -3.1 1,654,147 - GFF1625 0.56 -0.8 1,654,147 - GFF1625 0.56 -2.4 1,654,147 - GFF1625 0.56 -2.8 1,654,152 - GFF1625 0.57 -2.1 1,654,152 - GFF1625 0.57 -1.2 1,654,174 + GFF1625 0.60 -2.4 1,654,202 + GFF1625 0.65 -2.0 1,654,205 - GFF1625 0.65 -0.1 1,654,210 - GFF1625 0.66 -3.0 1,654,220 + GFF1625 0.67 -1.6 1,654,220 + GFF1625 0.67 -3.3 1,654,223 - GFF1625 0.68 -2.0 1,654,228 - GFF1625 0.69 -1.6 1,654,239 - GFF1625 0.70 -2.3 1,654,269 + GFF1625 0.75 -1.7 1,654,274 + GFF1625 0.76 -2.7 1,654,300 - GFF1625 0.80 -3.5 1,654,362 + GFF1625 0.89 -1.4 1,654,363 - GFF1625 0.90 -1.8 1,654,373 + -2.2 1,654,375 + +0.3 1,654,381 - -2.4 1,654,383 - -2.6 1,654,383 - -0.8 1,654,383 - -1.9 1,654,391 - -1.8 1,654,391 - -0.7 1,654,393 + -1.0 1,654,393 + -0.7 1,654,401 - -1.6 1,654,437 + -1.1 1,654,439 + +0.8 1,654,444 + -0.3 1,654,445 - -0.1 1,654,447 - -1.3 1,654,534 + -2.2 1,654,675 + GFF1626 0.12 -1.3 1,655,124 + GFF1626 0.48 -1.4 1,655,317 + GFF1626 0.63 +1.9 1,655,443 + GFF1626 0.73 -1.6 1,655,452 + GFF1626 0.74 -3.1 1,655,452 + GFF1626 0.74 +0.7 1,655,460 - GFF1626 0.74 +0.3 1,655,468 + GFF1626 0.75 -1.3 1,655,488 + GFF1626 0.77 -0.1 1,655,501 + GFF1626 0.78 -1.7 1,655,501 + GFF1626 0.78 +0.3 1,655,514 - GFF1626 0.79 -0.7 1,655,523 + GFF1626 0.80 -1.2 1,655,617 + GFF1626 0.87 -3.2 1,655,624 + GFF1626 0.88 +0.5 1,655,742 + -2.2 1,655,778 + -1.1 1,655,778 + +0.3 1,655,821 + +0.3 1,655,821 + -0.5 1,655,826 + -1.4 1,655,849 - +0.3 1,655,863 + -2.2 1,655,892 + GFF1627 0.13 -1.3 1,655,991 + GFF1627 0.26 -0.7 1,656,127 + GFF1627 0.42 -0.7 1,656,135 + GFF1627 0.43 -1.4 1,656,135 + GFF1627 0.43 -1.4 1,656,135 + GFF1627 0.43 -1.1 1,656,135 + GFF1627 0.43 -0.1 1,656,135 + GFF1627 0.43 -1.4 1,656,135 + GFF1627 0.43 -1.8 1,656,135 + GFF1627 0.43 -1.4 1,656,135 + GFF1627 0.43 -2.5 1,656,135 + GFF1627 0.43 -1.8 1,656,135 + GFF1627 0.43 -0.5 1,656,135 + GFF1627 0.43 -0.5 1,656,135 + GFF1627 0.43 -3.1 1,656,135 + GFF1627 0.43 -1.5 1,656,135 + GFF1627 0.43 -1.1 1,656,135 + GFF1627 0.43 +0.3 1,656,135 + GFF1627 0.43 -1.9 1,656,135 + GFF1627 0.43 -0.9 1,656,135 + GFF1627 0.43 +0.3 1,656,135 + GFF1627 0.43 -2.3 1,656,135 + GFF1627 0.43 -0.7 1,656,135 + GFF1627 0.43 -3.7 1,656,135 + GFF1627 0.43 +0.5 1,656,135 + GFF1627 0.43 -1.9 1,656,135 + GFF1627 0.43 -1.4 1,656,135 + GFF1627 0.43 -1.5 1,656,135 + GFF1627 0.43 -1.6 1,656,135 + GFF1627 0.43 -0.2 1,656,135 + GFF1627 0.43 -1.8 1,656,135 + GFF1627 0.43 -0.9 1,656,135 + GFF1627 0.43 -1.1 1,656,135 + GFF1627 0.43 +0.1 1,656,135 + GFF1627 0.43 -0.5 1,656,135 + GFF1627 0.43 -1.9 1,656,135 + GFF1627 0.43 -0.5 1,656,135 + GFF1627 0.43 -1.2 1,656,135 + GFF1627 0.43 -0.5 1,656,143 - GFF1627 0.44 -0.1 1,656,143 - GFF1627 0.44 +0.3 1,656,157 + GFF1627 0.46 -2.1 1,656,157 + GFF1627 0.46 -1.7 1,656,381 + GFF1627 0.73 -3.4 1,656,565 + +0.7
Or see this region's nucleotide sequence