Experiment: EMM_noCarbon with Sucrose and Putrescine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1581 and GFF1582 are separated by 10 nucleotides GFF1582 and GFF1583 are separated by 5 nucleotides GFF1583 and GFF1584 are separated by 128 nucleotides GFF1584 and GFF1585 are separated by 57 nucleotides
GFF1581 - UDP-glucose 4-epimerase (EC 5.1.3.2), at 1,611,339 to 1,612,340
GFF1581
GFF1582 - tRNA proofreading protein STM4549, at 1,612,351 to 1,612,827
GFF1582
GFF1583 - 6-phosphogluconolactonase (EC 3.1.1.31), eukaryotic type, at 1,612,833 to 1,613,525
GFF1583
GFF1584 - hypothetical protein, at 1,613,654 to 1,613,920
GFF1584
GFF1585 - Valine--pyruvate aminotransferase (EC 2.6.1.66), at 1,613,978 to 1,615,114
GFF1585
Position (kb)
1612
1613
1614 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 1611.833 kb on + strand, within GFF1581 at 1611.873 kb on + strand, within GFF1581 at 1611.873 kb on + strand, within GFF1581 at 1611.873 kb on - strand, within GFF1581 at 1611.901 kb on - strand, within GFF1581 at 1611.901 kb on - strand, within GFF1581 at 1611.901 kb on - strand, within GFF1581 at 1611.915 kb on - strand, within GFF1581 at 1611.917 kb on + strand, within GFF1581 at 1611.917 kb on + strand, within GFF1581 at 1611.917 kb on + strand, within GFF1581 at 1611.925 kb on - strand, within GFF1581 at 1611.925 kb on - strand, within GFF1581 at 1611.934 kb on + strand, within GFF1581 at 1611.944 kb on + strand, within GFF1581 at 1611.947 kb on - strand, within GFF1581 at 1611.950 kb on + strand, within GFF1581 at 1611.952 kb on - strand, within GFF1581 at 1611.958 kb on - strand, within GFF1581 at 1611.967 kb on - strand, within GFF1581 at 1611.997 kb on + strand, within GFF1581 at 1612.015 kb on + strand, within GFF1581 at 1612.020 kb on - strand, within GFF1581 at 1612.039 kb on + strand, within GFF1581 at 1612.110 kb on + strand, within GFF1581 at 1612.142 kb on - strand, within GFF1581 at 1612.142 kb on - strand, within GFF1581 at 1612.163 kb on + strand, within GFF1581 at 1612.181 kb on + strand, within GFF1581 at 1612.311 kb on - strand at 1612.311 kb on - strand at 1612.354 kb on + strand at 1612.362 kb on - strand at 1612.417 kb on + strand, within GFF1582 at 1612.419 kb on + strand, within GFF1582 at 1612.437 kb on + strand, within GFF1582 at 1612.519 kb on - strand, within GFF1582 at 1612.524 kb on - strand, within GFF1582 at 1612.524 kb on - strand, within GFF1582 at 1612.529 kb on - strand, within GFF1582 at 1612.529 kb on - strand, within GFF1582 at 1612.543 kb on + strand, within GFF1582 at 1612.546 kb on - strand, within GFF1582 at 1612.560 kb on + strand, within GFF1582 at 1612.568 kb on - strand, within GFF1582 at 1612.568 kb on - strand, within GFF1582 at 1612.592 kb on + strand, within GFF1582 at 1612.607 kb on + strand, within GFF1582 at 1612.607 kb on + strand, within GFF1582 at 1612.615 kb on - strand, within GFF1582 at 1612.616 kb on - strand, within GFF1582 at 1612.667 kb on - strand, within GFF1582 at 1612.667 kb on - strand, within GFF1582 at 1612.701 kb on + strand, within GFF1582 at 1612.701 kb on + strand, within GFF1582 at 1612.750 kb on - strand, within GFF1582 at 1612.790 kb on + strand at 1612.790 kb on + strand at 1612.797 kb on + strand at 1612.798 kb on - strand at 1612.800 kb on - strand at 1612.805 kb on - strand at 1612.823 kb on + strand at 1612.823 kb on + strand at 1612.828 kb on + strand at 1612.832 kb on - strand at 1612.836 kb on - strand at 1612.836 kb on - strand at 1612.842 kb on + strand at 1613.062 kb on - strand, within GFF1583 at 1613.198 kb on + strand, within GFF1583 at 1613.266 kb on - strand, within GFF1583 at 1613.300 kb on + strand, within GFF1583 at 1613.301 kb on + strand, within GFF1583 at 1613.309 kb on - strand, within GFF1583 at 1613.375 kb on - strand, within GFF1583 at 1613.518 kb on - strand at 1613.572 kb on + strand at 1613.585 kb on - strand at 1613.602 kb on + strand at 1613.605 kb on + strand at 1613.605 kb on + strand at 1613.613 kb on - strand at 1613.646 kb on + strand at 1613.668 kb on + strand at 1613.711 kb on + strand, within GFF1584 at 1613.756 kb on - strand, within GFF1584 at 1613.898 kb on + strand at 1613.906 kb on - strand at 1613.915 kb on + strand at 1613.939 kb on + strand at 1613.939 kb on + strand at 1613.952 kb on - strand at 1613.984 kb on + strand at 1613.989 kb on + strand at 1614.026 kb on + strand at 1614.034 kb on + strand at 1614.034 kb on + strand at 1614.034 kb on + strand at 1614.034 kb on + strand at 1614.042 kb on - strand at 1614.042 kb on - strand at 1614.042 kb on - strand at 1614.042 kb on - strand at 1614.042 kb on - strand at 1614.042 kb on - strand at 1614.051 kb on + strand at 1614.056 kb on + strand at 1614.059 kb on - strand at 1614.064 kb on - strand at 1614.087 kb on - strand at 1614.087 kb on - strand at 1614.087 kb on - strand at 1614.088 kb on - strand at 1614.092 kb on - strand, within GFF1585 at 1614.123 kb on + strand, within GFF1585 at 1614.127 kb on + strand, within GFF1585 at 1614.128 kb on + strand, within GFF1585 at 1614.133 kb on - strand, within GFF1585 at 1614.135 kb on - strand, within GFF1585 at 1614.140 kb on - strand, within GFF1585 at 1614.140 kb on - strand, within GFF1585 at 1614.159 kb on + strand, within GFF1585 at 1614.169 kb on + strand, within GFF1585 at 1614.187 kb on + strand, within GFF1585 at 1614.187 kb on + strand, within GFF1585 at 1614.187 kb on + strand, within GFF1585 at 1614.187 kb on + strand, within GFF1585 at 1614.187 kb on + strand, within GFF1585 at 1614.187 kb on + strand, within GFF1585 at 1614.187 kb on + strand, within GFF1585 at 1614.187 kb on + strand, within GFF1585 at 1614.195 kb on - strand, within GFF1585 at 1614.195 kb on - strand, within GFF1585 at 1614.195 kb on - strand, within GFF1585 at 1614.195 kb on - strand, within GFF1585 at 1614.195 kb on - strand, within GFF1585 at 1614.195 kb on - strand, within GFF1585 at 1614.195 kb on - strand, within GFF1585 at 1614.227 kb on - strand, within GFF1585 at 1614.241 kb on - strand, within GFF1585 at 1614.291 kb on - strand, within GFF1585 at 1614.357 kb on + strand, within GFF1585 at 1614.357 kb on + strand, within GFF1585 at 1614.357 kb on + strand, within GFF1585 at 1614.357 kb on + strand, within GFF1585 at 1614.357 kb on + strand, within GFF1585 at 1614.357 kb on + strand, within GFF1585 at 1614.357 kb on + strand, within GFF1585 at 1614.357 kb on + strand, within GFF1585 at 1614.357 kb on + strand, within GFF1585 at 1614.357 kb on + strand, within GFF1585 at 1614.365 kb on - strand, within GFF1585 at 1614.365 kb on - strand, within GFF1585 at 1614.365 kb on - strand, within GFF1585 at 1614.365 kb on - strand, within GFF1585 at 1614.367 kb on + strand, within GFF1585 at 1614.403 kb on + strand, within GFF1585 at 1614.403 kb on + strand, within GFF1585 at 1614.403 kb on + strand, within GFF1585 at 1614.411 kb on - strand, within GFF1585 at 1614.446 kb on + strand, within GFF1585 at 1614.446 kb on + strand, within GFF1585 at 1614.447 kb on + strand, within GFF1585 at 1614.455 kb on - strand, within GFF1585 at 1614.466 kb on + strand, within GFF1585 at 1614.523 kb on - strand, within GFF1585
Per-strain Table
Position Strand Gene LocusTag Fraction EMM_noCarbon with Sucrose and Putrescine remove 1,611,833 + GFF1581 0.49 +0.4 1,611,873 + GFF1581 0.53 +0.1 1,611,873 + GFF1581 0.53 +0.9 1,611,873 - GFF1581 0.53 +1.3 1,611,901 - GFF1581 0.56 -0.4 1,611,901 - GFF1581 0.56 -1.5 1,611,901 - GFF1581 0.56 +0.1 1,611,915 - GFF1581 0.57 +1.1 1,611,917 + GFF1581 0.58 +1.4 1,611,917 + GFF1581 0.58 -0.5 1,611,917 + GFF1581 0.58 -0.1 1,611,925 - GFF1581 0.58 -1.3 1,611,925 - GFF1581 0.58 +0.6 1,611,934 + GFF1581 0.59 +0.7 1,611,944 + GFF1581 0.60 -0.4 1,611,947 - GFF1581 0.61 +0.6 1,611,950 + GFF1581 0.61 +1.5 1,611,952 - GFF1581 0.61 -1.1 1,611,958 - GFF1581 0.62 +2.3 1,611,967 - GFF1581 0.63 -0.2 1,611,997 + GFF1581 0.66 -1.7 1,612,015 + GFF1581 0.67 +0.2 1,612,020 - GFF1581 0.68 -0.1 1,612,039 + GFF1581 0.70 +2.1 1,612,110 + GFF1581 0.77 +3.1 1,612,142 - GFF1581 0.80 -0.5 1,612,142 - GFF1581 0.80 +0.5 1,612,163 + GFF1581 0.82 -0.9 1,612,181 + GFF1581 0.84 +1.7 1,612,311 - +1.6 1,612,311 - +1.8 1,612,354 + -0.5 1,612,362 - +1.1 1,612,417 + GFF1582 0.14 +0.5 1,612,419 + GFF1582 0.14 -0.2 1,612,437 + GFF1582 0.18 +0.6 1,612,519 - GFF1582 0.35 +0.4 1,612,524 - GFF1582 0.36 -0.2 1,612,524 - GFF1582 0.36 -1.6 1,612,529 - GFF1582 0.37 -0.9 1,612,529 - GFF1582 0.37 -1.4 1,612,543 + GFF1582 0.40 +1.5 1,612,546 - GFF1582 0.41 -1.1 1,612,560 + GFF1582 0.44 -1.5 1,612,568 - GFF1582 0.45 -2.6 1,612,568 - GFF1582 0.45 -0.3 1,612,592 + GFF1582 0.51 -1.4 1,612,607 + GFF1582 0.54 -0.3 1,612,607 + GFF1582 0.54 -1.3 1,612,615 - GFF1582 0.55 +1.0 1,612,616 - GFF1582 0.56 -0.1 1,612,667 - GFF1582 0.66 -2.1 1,612,667 - GFF1582 0.66 +0.5 1,612,701 + GFF1582 0.73 +0.2 1,612,701 + GFF1582 0.73 -0.4 1,612,750 - GFF1582 0.84 +1.3 1,612,790 + -0.2 1,612,790 + -2.4 1,612,797 + -0.7 1,612,798 - -0.2 1,612,800 - -1.3 1,612,805 - +0.0 1,612,823 + -0.4 1,612,823 + +0.2 1,612,828 + -1.2 1,612,832 - -0.7 1,612,836 - +0.3 1,612,836 - +1.0 1,612,842 + -2.4 1,613,062 - GFF1583 0.33 -0.1 1,613,198 + GFF1583 0.53 -1.5 1,613,266 - GFF1583 0.62 -0.5 1,613,300 + GFF1583 0.67 -0.7 1,613,301 + GFF1583 0.68 +1.3 1,613,309 - GFF1583 0.69 -1.9 1,613,375 - GFF1583 0.78 -1.4 1,613,518 - +0.7 1,613,572 + +0.0 1,613,585 - +0.3 1,613,602 + +1.1 1,613,605 + -0.2 1,613,605 + -1.2 1,613,613 - -1.7 1,613,646 + +0.2 1,613,668 + -1.8 1,613,711 + GFF1584 0.21 -0.2 1,613,756 - GFF1584 0.38 +0.3 1,613,898 + -0.7 1,613,906 - +0.5 1,613,915 + +1.2 1,613,939 + -0.5 1,613,939 + -0.8 1,613,952 - -2.0 1,613,984 + -0.4 1,613,989 + +0.1 1,614,026 + +0.3 1,614,034 + -1.2 1,614,034 + +1.7 1,614,034 + -0.5 1,614,034 + +0.6 1,614,042 - -1.1 1,614,042 - +0.3 1,614,042 - -0.1 1,614,042 - +0.7 1,614,042 - -0.4 1,614,042 - +1.4 1,614,051 + +0.3 1,614,056 + +0.4 1,614,059 - -0.6 1,614,064 - +0.9 1,614,087 - -0.1 1,614,087 - -0.6 1,614,087 - -0.7 1,614,088 - +0.8 1,614,092 - GFF1585 0.10 +2.3 1,614,123 + GFF1585 0.13 +0.6 1,614,127 + GFF1585 0.13 -2.1 1,614,128 + GFF1585 0.13 +0.2 1,614,133 - GFF1585 0.14 -1.7 1,614,135 - GFF1585 0.14 +0.7 1,614,140 - GFF1585 0.14 -1.3 1,614,140 - GFF1585 0.14 -0.4 1,614,159 + GFF1585 0.16 -0.1 1,614,169 + GFF1585 0.17 +1.1 1,614,187 + GFF1585 0.18 -1.4 1,614,187 + GFF1585 0.18 +0.5 1,614,187 + GFF1585 0.18 +0.2 1,614,187 + GFF1585 0.18 -0.5 1,614,187 + GFF1585 0.18 -1.0 1,614,187 + GFF1585 0.18 +0.3 1,614,187 + GFF1585 0.18 +1.6 1,614,187 + GFF1585 0.18 -0.1 1,614,195 - GFF1585 0.19 +0.9 1,614,195 - GFF1585 0.19 -0.3 1,614,195 - GFF1585 0.19 -0.3 1,614,195 - GFF1585 0.19 -0.0 1,614,195 - GFF1585 0.19 -1.2 1,614,195 - GFF1585 0.19 -0.7 1,614,195 - GFF1585 0.19 +0.1 1,614,227 - GFF1585 0.22 +1.1 1,614,241 - GFF1585 0.23 -0.2 1,614,291 - GFF1585 0.28 -2.1 1,614,357 + GFF1585 0.33 +0.7 1,614,357 + GFF1585 0.33 +0.2 1,614,357 + GFF1585 0.33 -1.1 1,614,357 + GFF1585 0.33 -0.5 1,614,357 + GFF1585 0.33 -1.2 1,614,357 + GFF1585 0.33 -2.8 1,614,357 + GFF1585 0.33 +1.1 1,614,357 + GFF1585 0.33 -2.9 1,614,357 + GFF1585 0.33 +0.5 1,614,357 + GFF1585 0.33 +0.2 1,614,365 - GFF1585 0.34 -0.0 1,614,365 - GFF1585 0.34 +0.1 1,614,365 - GFF1585 0.34 -0.7 1,614,365 - GFF1585 0.34 -0.5 1,614,367 + GFF1585 0.34 +0.9 1,614,403 + GFF1585 0.37 -0.2 1,614,403 + GFF1585 0.37 +0.0 1,614,403 + GFF1585 0.37 -0.3 1,614,411 - GFF1585 0.38 -0.4 1,614,446 + GFF1585 0.41 -1.4 1,614,446 + GFF1585 0.41 -0.5 1,614,447 + GFF1585 0.41 -0.5 1,614,455 - GFF1585 0.42 -0.2 1,614,466 + GFF1585 0.43 -1.3 1,614,523 - GFF1585 0.48 +1.4
Or see this region's nucleotide sequence