Experiment: EMM_noCarbon with Sucrose and Putrescine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1072 and GFF1073 are separated by 38 nucleotides GFF1073 and GFF1074 are separated by 135 nucleotides GFF1074 and GFF1075 overlap by 4 nucleotides
GFF1072 - protein of unknown function DUF1345, at 1,079,963 to 1,080,598
GFF1072
GFF1073 - hypothetical protein, at 1,080,637 to 1,081,212
GFF1073
GFF1074 - Holliday junction ATP-dependent DNA helicase RuvA (EC 3.6.4.12), at 1,081,348 to 1,081,959
GFF1074
GFF1075 - Holliday junction ATP-dependent DNA helicase RuvB (EC 3.6.4.12), at 1,081,956 to 1,082,978
GFF1075
Position (kb)
1081
1082 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1080.384 kb on + strand, within GFF1072 at 1080.386 kb on + strand, within GFF1072 at 1080.391 kb on + strand, within GFF1072 at 1080.404 kb on - strand, within GFF1072 at 1080.418 kb on + strand, within GFF1072 at 1080.418 kb on + strand, within GFF1072 at 1080.469 kb on + strand, within GFF1072 at 1080.469 kb on + strand, within GFF1072 at 1080.477 kb on - strand, within GFF1072 at 1080.479 kb on + strand, within GFF1072 at 1080.487 kb on - strand, within GFF1072 at 1080.487 kb on - strand, within GFF1072 at 1080.495 kb on + strand, within GFF1072 at 1080.498 kb on - strand, within GFF1072 at 1080.498 kb on - strand, within GFF1072 at 1080.498 kb on - strand, within GFF1072 at 1080.503 kb on - strand, within GFF1072 at 1080.503 kb on - strand, within GFF1072 at 1080.525 kb on + strand, within GFF1072 at 1080.540 kb on + strand at 1080.546 kb on - strand at 1080.546 kb on - strand at 1080.553 kb on - strand at 1080.589 kb on - strand at 1080.589 kb on - strand at 1080.611 kb on - strand at 1080.651 kb on + strand at 1080.655 kb on - strand at 1080.687 kb on - strand at 1080.707 kb on + strand, within GFF1073 at 1080.709 kb on + strand, within GFF1073 at 1080.716 kb on + strand, within GFF1073 at 1080.717 kb on - strand, within GFF1073 at 1080.722 kb on - strand, within GFF1073 at 1080.786 kb on + strand, within GFF1073 at 1080.814 kb on - strand, within GFF1073 at 1080.827 kb on + strand, within GFF1073 at 1080.834 kb on - strand, within GFF1073 at 1080.893 kb on - strand, within GFF1073 at 1080.893 kb on - strand, within GFF1073 at 1080.893 kb on - strand, within GFF1073 at 1080.931 kb on + strand, within GFF1073 at 1080.939 kb on - strand, within GFF1073 at 1080.956 kb on + strand, within GFF1073 at 1080.992 kb on + strand, within GFF1073 at 1081.013 kb on + strand, within GFF1073 at 1081.033 kb on - strand, within GFF1073 at 1081.067 kb on - strand, within GFF1073 at 1081.067 kb on - strand, within GFF1073 at 1081.067 kb on - strand, within GFF1073 at 1081.077 kb on + strand, within GFF1073 at 1081.080 kb on + strand, within GFF1073 at 1081.085 kb on - strand, within GFF1073 at 1081.085 kb on - strand, within GFF1073 at 1081.100 kb on + strand, within GFF1073 at 1081.125 kb on + strand, within GFF1073 at 1081.133 kb on - strand, within GFF1073 at 1081.153 kb on + strand, within GFF1073 at 1081.158 kb on - strand at 1081.161 kb on - strand at 1081.202 kb on + strand at 1081.202 kb on + strand at 1081.210 kb on - strand at 1081.256 kb on - strand at 1081.308 kb on + strand at 1081.330 kb on + strand at 1081.547 kb on + strand, within GFF1074 at 1081.555 kb on - strand, within GFF1074 at 1081.565 kb on + strand, within GFF1074 at 1081.580 kb on + strand, within GFF1074 at 1081.742 kb on - strand, within GFF1074 at 1082.260 kb on - strand, within GFF1075 at 1082.277 kb on - strand, within GFF1075 at 1082.300 kb on + strand, within GFF1075 at 1082.324 kb on + strand, within GFF1075 at 1082.332 kb on - strand, within GFF1075 at 1082.345 kb on - strand, within GFF1075 at 1082.395 kb on + strand, within GFF1075 at 1082.403 kb on - strand, within GFF1075 at 1082.495 kb on + strand, within GFF1075 at 1082.585 kb on - strand, within GFF1075 at 1082.937 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction EMM_noCarbon with Sucrose and Putrescine remove 1,080,384 + GFF1072 0.66 -0.7 1,080,386 + GFF1072 0.67 -0.8 1,080,391 + GFF1072 0.67 -1.3 1,080,404 - GFF1072 0.69 -0.0 1,080,418 + GFF1072 0.72 -0.4 1,080,418 + GFF1072 0.72 -1.0 1,080,469 + GFF1072 0.80 +1.8 1,080,469 + GFF1072 0.80 -0.2 1,080,477 - GFF1072 0.81 -0.7 1,080,479 + GFF1072 0.81 -1.5 1,080,487 - GFF1072 0.82 +1.3 1,080,487 - GFF1072 0.82 -1.6 1,080,495 + GFF1072 0.84 +0.7 1,080,498 - GFF1072 0.84 +0.1 1,080,498 - GFF1072 0.84 -1.5 1,080,498 - GFF1072 0.84 +0.1 1,080,503 - GFF1072 0.85 -0.2 1,080,503 - GFF1072 0.85 -0.2 1,080,525 + GFF1072 0.88 +0.5 1,080,540 + -0.8 1,080,546 - +0.1 1,080,546 - +2.0 1,080,553 - -1.1 1,080,589 - +0.4 1,080,589 - +1.2 1,080,611 - -2.8 1,080,651 + +0.3 1,080,655 - -1.3 1,080,687 - -0.7 1,080,707 + GFF1073 0.12 +0.4 1,080,709 + GFF1073 0.12 +0.6 1,080,716 + GFF1073 0.14 +1.2 1,080,717 - GFF1073 0.14 +0.3 1,080,722 - GFF1073 0.15 +0.5 1,080,786 + GFF1073 0.26 -0.2 1,080,814 - GFF1073 0.31 +1.0 1,080,827 + GFF1073 0.33 +0.2 1,080,834 - GFF1073 0.34 -1.1 1,080,893 - GFF1073 0.44 +0.3 1,080,893 - GFF1073 0.44 +1.9 1,080,893 - GFF1073 0.44 -0.4 1,080,931 + GFF1073 0.51 +0.8 1,080,939 - GFF1073 0.52 +1.0 1,080,956 + GFF1073 0.55 +0.9 1,080,992 + GFF1073 0.62 +0.3 1,081,013 + GFF1073 0.65 +0.2 1,081,033 - GFF1073 0.69 -0.9 1,081,067 - GFF1073 0.75 +0.3 1,081,067 - GFF1073 0.75 -1.8 1,081,067 - GFF1073 0.75 +0.3 1,081,077 + GFF1073 0.76 +0.2 1,081,080 + GFF1073 0.77 -1.4 1,081,085 - GFF1073 0.78 +0.6 1,081,085 - GFF1073 0.78 +0.2 1,081,100 + GFF1073 0.80 -0.2 1,081,125 + GFF1073 0.85 +0.8 1,081,133 - GFF1073 0.86 +0.6 1,081,153 + GFF1073 0.90 +0.5 1,081,158 - -1.3 1,081,161 - -0.1 1,081,202 + +0.1 1,081,202 + +0.3 1,081,210 - -0.8 1,081,256 - -1.9 1,081,308 + +0.2 1,081,330 + -0.0 1,081,547 + GFF1074 0.33 -0.9 1,081,555 - GFF1074 0.34 -0.7 1,081,565 + GFF1074 0.35 +1.8 1,081,580 + GFF1074 0.38 -1.2 1,081,742 - GFF1074 0.64 -0.7 1,082,260 - GFF1075 0.30 +1.3 1,082,277 - GFF1075 0.31 -0.0 1,082,300 + GFF1075 0.34 +1.3 1,082,324 + GFF1075 0.36 -0.0 1,082,332 - GFF1075 0.37 -2.2 1,082,345 - GFF1075 0.38 +0.5 1,082,395 + GFF1075 0.43 -0.3 1,082,403 - GFF1075 0.44 +0.3 1,082,495 + GFF1075 0.53 +0.3 1,082,585 - GFF1075 0.61 -0.5 1,082,937 - +0.5
Or see this region's nucleotide sequence