Experiment: EMM_noCarbon with Sucrose and Putrescine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4947 and GFF4948 are separated by 15 nucleotides GFF4948 and GFF4949 are separated by 17 nucleotides GFF4949 and GFF4950 are separated by 51 nucleotides GFF4950 and GFF4951 are separated by 59 nucleotides
GFF4947 - Glutamate N-acetyltransferase (EC 2.3.1.35) @ N-acetylglutamate synthase (EC 2.3.1.1), at 4,939,532 to 4,940,770
GFF4947
GFF4948 - Inositol-1-monophosphatase (EC 3.1.3.25), at 4,940,786 to 4,941,580
GFF4948
GFF4949 - Uncharacterized glutathione S-transferase-like protein, at 4,941,598 to 4,942,209
GFF4949
GFF4950 - Thioredoxin, at 4,942,261 to 4,942,581
GFF4950
GFF4951 - FIG061771: ATP-dependent nuclease subunit A, at 4,942,641 to 4,946,051
GFF4951
Position (kb)
4941
4942
4943 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 4940.607 kb on + strand, within GFF4947 at 4940.622 kb on + strand, within GFF4947 at 4940.622 kb on + strand, within GFF4947 at 4940.648 kb on + strand at 4940.650 kb on + strand at 4940.650 kb on + strand at 4940.658 kb on - strand at 4940.662 kb on + strand at 4940.665 kb on - strand at 4940.665 kb on - strand at 4940.670 kb on - strand at 4940.682 kb on + strand at 4940.690 kb on - strand at 4940.693 kb on + strand at 4940.701 kb on - strand at 4940.711 kb on + strand at 4940.718 kb on + strand at 4940.719 kb on - strand at 4940.736 kb on + strand at 4940.736 kb on - strand at 4940.741 kb on + strand at 4940.749 kb on - strand at 4940.749 kb on - strand at 4940.766 kb on + strand at 4940.787 kb on + strand at 4940.787 kb on + strand at 4940.787 kb on + strand at 4940.787 kb on - strand at 4940.795 kb on - strand at 4940.826 kb on - strand at 4940.827 kb on + strand at 4940.836 kb on - strand at 4940.851 kb on - strand at 4940.910 kb on - strand, within GFF4948 at 4940.910 kb on - strand, within GFF4948 at 4940.948 kb on + strand, within GFF4948 at 4940.957 kb on - strand, within GFF4948 at 4940.970 kb on + strand, within GFF4948 at 4940.995 kb on - strand, within GFF4948 at 4941.014 kb on + strand, within GFF4948 at 4941.014 kb on + strand, within GFF4948 at 4941.017 kb on - strand, within GFF4948 at 4941.022 kb on - strand, within GFF4948 at 4941.022 kb on - strand, within GFF4948 at 4941.022 kb on - strand, within GFF4948 at 4941.044 kb on - strand, within GFF4948 at 4941.044 kb on - strand, within GFF4948 at 4941.074 kb on - strand, within GFF4948 at 4941.086 kb on + strand, within GFF4948 at 4941.118 kb on + strand, within GFF4948 at 4941.118 kb on + strand, within GFF4948 at 4941.118 kb on + strand, within GFF4948 at 4941.126 kb on - strand, within GFF4948 at 4941.126 kb on - strand, within GFF4948 at 4941.187 kb on - strand, within GFF4948 at 4941.188 kb on - strand, within GFF4948 at 4941.193 kb on - strand, within GFF4948 at 4941.193 kb on - strand, within GFF4948 at 4941.193 kb on - strand, within GFF4948 at 4941.193 kb on - strand, within GFF4948 at 4941.303 kb on - strand, within GFF4948 at 4941.315 kb on - strand, within GFF4948 at 4941.331 kb on - strand, within GFF4948 at 4941.334 kb on + strand, within GFF4948 at 4941.374 kb on - strand, within GFF4948 at 4941.375 kb on - strand, within GFF4948 at 4941.380 kb on - strand, within GFF4948 at 4941.386 kb on - strand, within GFF4948 at 4941.396 kb on - strand, within GFF4948 at 4941.413 kb on + strand, within GFF4948 at 4941.418 kb on + strand, within GFF4948 at 4941.420 kb on + strand, within GFF4948 at 4941.420 kb on + strand, within GFF4948 at 4941.421 kb on - strand, within GFF4948 at 4941.421 kb on - strand, within GFF4948 at 4941.426 kb on - strand, within GFF4948 at 4941.426 kb on - strand, within GFF4948 at 4941.426 kb on - strand, within GFF4948 at 4941.426 kb on - strand, within GFF4948 at 4941.453 kb on - strand, within GFF4948 at 4941.464 kb on - strand, within GFF4948 at 4941.465 kb on - strand, within GFF4948 at 4941.466 kb on - strand, within GFF4948 at 4941.492 kb on + strand, within GFF4948 at 4941.503 kb on + strand at 4941.518 kb on + strand at 4941.521 kb on - strand at 4941.523 kb on + strand at 4941.554 kb on - strand at 4941.560 kb on + strand at 4941.568 kb on - strand at 4941.588 kb on - strand at 4941.606 kb on + strand at 4941.607 kb on + strand at 4941.607 kb on + strand at 4941.614 kb on - strand at 4941.695 kb on + strand, within GFF4949 at 4941.695 kb on + strand, within GFF4949 at 4941.700 kb on + strand, within GFF4949 at 4941.700 kb on + strand, within GFF4949 at 4941.700 kb on + strand, within GFF4949 at 4941.703 kb on - strand, within GFF4949 at 4941.708 kb on - strand, within GFF4949 at 4941.711 kb on + strand, within GFF4949 at 4941.751 kb on - strand, within GFF4949 at 4941.772 kb on + strand, within GFF4949 at 4941.806 kb on + strand, within GFF4949 at 4941.814 kb on - strand, within GFF4949 at 4941.826 kb on - strand, within GFF4949 at 4941.832 kb on + strand, within GFF4949 at 4941.842 kb on - strand, within GFF4949 at 4941.856 kb on - strand, within GFF4949 at 4941.907 kb on + strand, within GFF4949 at 4941.907 kb on + strand, within GFF4949 at 4941.920 kb on + strand, within GFF4949 at 4941.920 kb on + strand, within GFF4949 at 4941.944 kb on + strand, within GFF4949 at 4941.944 kb on + strand, within GFF4949 at 4941.966 kb on - strand, within GFF4949 at 4941.989 kb on + strand, within GFF4949 at 4942.009 kb on + strand, within GFF4949 at 4942.041 kb on + strand, within GFF4949 at 4942.095 kb on + strand, within GFF4949 at 4942.096 kb on - strand, within GFF4949 at 4942.103 kb on - strand, within GFF4949 at 4942.147 kb on + strand, within GFF4949 at 4942.148 kb on + strand at 4942.153 kb on + strand at 4942.155 kb on - strand at 4942.163 kb on + strand at 4942.168 kb on + strand at 4942.178 kb on + strand at 4942.194 kb on - strand at 4942.599 kb on - strand at 4942.601 kb on - strand at 4942.610 kb on - strand at 4942.626 kb on - strand at 4942.627 kb on - strand at 4942.627 kb on - strand at 4942.637 kb on - strand at 4942.711 kb on + strand at 4942.716 kb on - strand at 4942.726 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction EMM_noCarbon with Sucrose and Putrescine remove 4,940,607 + GFF4947 0.87 -1.1 4,940,622 + GFF4947 0.88 -1.9 4,940,622 + GFF4947 0.88 -2.3 4,940,648 + -2.2 4,940,650 + -2.4 4,940,650 + -0.6 4,940,658 - -2.7 4,940,662 + -1.8 4,940,665 - -2.9 4,940,665 - -1.1 4,940,670 - -2.1 4,940,682 + -2.1 4,940,690 - -0.8 4,940,693 + -2.3 4,940,701 - -0.5 4,940,711 + -2.2 4,940,718 + -1.7 4,940,719 - -1.9 4,940,736 + -2.4 4,940,736 - -3.7 4,940,741 + -2.5 4,940,749 - -1.4 4,940,749 - -1.0 4,940,766 + -0.3 4,940,787 + -0.8 4,940,787 + +0.9 4,940,787 + +0.3 4,940,787 - -0.5 4,940,795 - -0.2 4,940,826 - -1.5 4,940,827 + -0.6 4,940,836 - -1.1 4,940,851 - -1.0 4,940,910 - GFF4948 0.16 -0.4 4,940,910 - GFF4948 0.16 -1.1 4,940,948 + GFF4948 0.20 -1.2 4,940,957 - GFF4948 0.22 -1.7 4,940,970 + GFF4948 0.23 -0.9 4,940,995 - GFF4948 0.26 +1.2 4,941,014 + GFF4948 0.29 -0.3 4,941,014 + GFF4948 0.29 -0.5 4,941,017 - GFF4948 0.29 -1.3 4,941,022 - GFF4948 0.30 +0.3 4,941,022 - GFF4948 0.30 -2.4 4,941,022 - GFF4948 0.30 +0.3 4,941,044 - GFF4948 0.32 +0.7 4,941,044 - GFF4948 0.32 +0.0 4,941,074 - GFF4948 0.36 -0.5 4,941,086 + GFF4948 0.38 -0.7 4,941,118 + GFF4948 0.42 -1.0 4,941,118 + GFF4948 0.42 -1.2 4,941,118 + GFF4948 0.42 +0.4 4,941,126 - GFF4948 0.43 +0.8 4,941,126 - GFF4948 0.43 +0.2 4,941,187 - GFF4948 0.50 -2.7 4,941,188 - GFF4948 0.51 +0.1 4,941,193 - GFF4948 0.51 +0.2 4,941,193 - GFF4948 0.51 -0.3 4,941,193 - GFF4948 0.51 -2.1 4,941,193 - GFF4948 0.51 -0.3 4,941,303 - GFF4948 0.65 -1.0 4,941,315 - GFF4948 0.67 -0.6 4,941,331 - GFF4948 0.69 -0.9 4,941,334 + GFF4948 0.69 -0.6 4,941,374 - GFF4948 0.74 -1.9 4,941,375 - GFF4948 0.74 -1.2 4,941,380 - GFF4948 0.75 -0.7 4,941,386 - GFF4948 0.75 -0.5 4,941,396 - GFF4948 0.77 -0.5 4,941,413 + GFF4948 0.79 -0.3 4,941,418 + GFF4948 0.79 -1.4 4,941,420 + GFF4948 0.80 +0.9 4,941,420 + GFF4948 0.80 +1.2 4,941,421 - GFF4948 0.80 -0.2 4,941,421 - GFF4948 0.80 -0.6 4,941,426 - GFF4948 0.81 +0.0 4,941,426 - GFF4948 0.81 +0.3 4,941,426 - GFF4948 0.81 +0.7 4,941,426 - GFF4948 0.81 -0.4 4,941,453 - GFF4948 0.84 -0.6 4,941,464 - GFF4948 0.85 +0.1 4,941,465 - GFF4948 0.85 -0.7 4,941,466 - GFF4948 0.86 +0.1 4,941,492 + GFF4948 0.89 -1.8 4,941,503 + -0.0 4,941,518 + -0.1 4,941,521 - -0.0 4,941,523 + +0.8 4,941,554 - +1.2 4,941,560 + -0.1 4,941,568 - -0.2 4,941,588 - -0.1 4,941,606 + -1.2 4,941,607 + -1.3 4,941,607 + +0.4 4,941,614 - -0.1 4,941,695 + GFF4949 0.16 -1.0 4,941,695 + GFF4949 0.16 -1.9 4,941,700 + GFF4949 0.17 -0.1 4,941,700 + GFF4949 0.17 -1.3 4,941,700 + GFF4949 0.17 +0.4 4,941,703 - GFF4949 0.17 -2.1 4,941,708 - GFF4949 0.18 -1.4 4,941,711 + GFF4949 0.18 -1.3 4,941,751 - GFF4949 0.25 +0.3 4,941,772 + GFF4949 0.28 -2.2 4,941,806 + GFF4949 0.34 -0.9 4,941,814 - GFF4949 0.35 -0.4 4,941,826 - GFF4949 0.37 -2.0 4,941,832 + GFF4949 0.38 -1.4 4,941,842 - GFF4949 0.40 -0.2 4,941,856 - GFF4949 0.42 -1.5 4,941,907 + GFF4949 0.50 -1.6 4,941,907 + GFF4949 0.50 -1.2 4,941,920 + GFF4949 0.53 -1.0 4,941,920 + GFF4949 0.53 -0.6 4,941,944 + GFF4949 0.57 -1.1 4,941,944 + GFF4949 0.57 -2.8 4,941,966 - GFF4949 0.60 -0.8 4,941,989 + GFF4949 0.64 -2.2 4,942,009 + GFF4949 0.67 -0.9 4,942,041 + GFF4949 0.72 -0.3 4,942,095 + GFF4949 0.81 -0.5 4,942,096 - GFF4949 0.81 +0.7 4,942,103 - GFF4949 0.83 -2.7 4,942,147 + GFF4949 0.90 -0.5 4,942,148 + -1.1 4,942,153 + -2.8 4,942,155 - +1.2 4,942,163 + +0.2 4,942,168 + +0.9 4,942,178 + -0.5 4,942,194 - -0.3 4,942,599 - -1.9 4,942,601 - +1.0 4,942,610 - -1.9 4,942,626 - -0.2 4,942,627 - -0.7 4,942,627 - -1.8 4,942,637 - +0.3 4,942,711 + -0.2 4,942,716 - +0.6 4,942,726 - -0.2
Or see this region's nucleotide sequence