Experiment: EMM_noCarbon with Sucrose and Putrescine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4162 and GFF4163 are separated by 175 nucleotides GFF4163 and GFF4164 overlap by 8 nucleotides GFF4164 and GFF4165 overlap by 14 nucleotides GFF4165 and GFF4166 are separated by 130 nucleotides GFF4166 and GFF4167 are separated by 71 nucleotides
GFF4162 - Cell wall hydrolyses involved in spore germination, at 4,185,187 to 4,185,858
GFF4162
GFF4163 - hypothetical protein, at 4,186,034 to 4,186,384
GFF4163
GFF4164 - Uncharacterized protein, possibly involved in aromatic compounds catabolism, at 4,186,377 to 4,186,847
GFF4164
GFF4165 - PTS IIA-like nitrogen-regulatory protein PtsN, at 4,186,834 to 4,187,301
GFF4165
GFF4166 - Ribosome hibernation promoting factor Hpf, at 4,187,432 to 4,188,028
GFF4166
GFF4167 - DNA polymerase III epsilon subunit (EC 2.7.7.7), at 4,188,100 to 4,188,801
GFF4167
Position (kb)
4186
4187
4188 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 4185.841 kb on + strand at 4185.849 kb on - strand at 4185.861 kb on - strand at 4185.913 kb on - strand at 4185.918 kb on + strand at 4185.929 kb on + strand at 4185.929 kb on + strand at 4185.929 kb on + strand at 4185.935 kb on + strand at 4185.937 kb on - strand at 4185.937 kb on - strand at 4185.980 kb on + strand at 4186.009 kb on - strand at 4186.018 kb on + strand at 4186.024 kb on - strand at 4186.026 kb on - strand at 4186.060 kb on + strand at 4186.084 kb on + strand, within GFF4163 at 4186.102 kb on + strand, within GFF4163 at 4186.102 kb on + strand, within GFF4163 at 4186.118 kb on - strand, within GFF4163 at 4186.128 kb on - strand, within GFF4163 at 4186.141 kb on + strand, within GFF4163 at 4186.141 kb on + strand, within GFF4163 at 4186.153 kb on + strand, within GFF4163 at 4186.153 kb on + strand, within GFF4163 at 4186.159 kb on - strand, within GFF4163 at 4186.159 kb on - strand, within GFF4163 at 4186.161 kb on + strand, within GFF4163 at 4186.161 kb on - strand, within GFF4163 at 4186.170 kb on + strand, within GFF4163 at 4186.172 kb on - strand, within GFF4163 at 4186.173 kb on - strand, within GFF4163 at 4186.173 kb on - strand, within GFF4163 at 4186.183 kb on - strand, within GFF4163 at 4186.188 kb on - strand, within GFF4163 at 4186.207 kb on - strand, within GFF4163 at 4186.233 kb on + strand, within GFF4163 at 4186.245 kb on + strand, within GFF4163 at 4186.245 kb on + strand, within GFF4163 at 4186.245 kb on + strand, within GFF4163 at 4186.253 kb on - strand, within GFF4163 at 4186.253 kb on - strand, within GFF4163 at 4186.255 kb on + strand, within GFF4163 at 4186.255 kb on + strand, within GFF4163 at 4186.255 kb on + strand, within GFF4163 at 4186.255 kb on + strand, within GFF4163 at 4186.255 kb on + strand, within GFF4163 at 4186.255 kb on + strand, within GFF4163 at 4186.263 kb on - strand, within GFF4163 at 4186.263 kb on - strand, within GFF4163 at 4186.263 kb on - strand, within GFF4163 at 4186.263 kb on - strand, within GFF4163 at 4186.278 kb on + strand, within GFF4163 at 4186.278 kb on + strand, within GFF4163 at 4186.286 kb on - strand, within GFF4163 at 4186.286 kb on - strand, within GFF4163 at 4186.320 kb on + strand, within GFF4163 at 4186.328 kb on - strand, within GFF4163 at 4186.330 kb on - strand, within GFF4163 at 4186.345 kb on - strand, within GFF4163 at 4186.350 kb on - strand at 4186.352 kb on + strand at 4186.352 kb on + strand at 4186.360 kb on - strand at 4186.360 kb on - strand at 4186.360 kb on - strand at 4186.360 kb on - strand at 4186.360 kb on - strand at 4186.360 kb on - strand at 4186.360 kb on - strand at 4186.360 kb on - strand at 4186.360 kb on - strand at 4186.360 kb on - strand at 4186.368 kb on + strand at 4186.373 kb on + strand at 4186.373 kb on + strand at 4186.373 kb on + strand at 4186.373 kb on + strand at 4186.373 kb on + strand at 4186.373 kb on + strand at 4186.376 kb on - strand at 4186.381 kb on - strand at 4186.381 kb on - strand at 4186.381 kb on - strand at 4186.381 kb on - strand at 4186.381 kb on - strand at 4186.387 kb on + strand at 4186.821 kb on - strand at 4186.826 kb on - strand at 4186.826 kb on - strand at 4186.826 kb on - strand at 4186.831 kb on + strand at 4186.849 kb on - strand at 4186.851 kb on + strand at 4186.859 kb on - strand at 4186.859 kb on - strand at 4186.859 kb on - strand at 4186.890 kb on + strand, within GFF4165 at 4186.890 kb on + strand, within GFF4165 at 4186.890 kb on + strand, within GFF4165 at 4186.898 kb on - strand, within GFF4165 at 4186.898 kb on - strand, within GFF4165 at 4186.915 kb on - strand, within GFF4165 at 4186.925 kb on - strand, within GFF4165 at 4186.943 kb on + strand, within GFF4165 at 4186.946 kb on - strand, within GFF4165 at 4186.965 kb on - strand, within GFF4165 at 4186.973 kb on + strand, within GFF4165 at 4186.981 kb on - strand, within GFF4165 at 4186.981 kb on - strand, within GFF4165 at 4186.993 kb on - strand, within GFF4165 at 4187.006 kb on + strand, within GFF4165 at 4187.014 kb on - strand, within GFF4165 at 4187.030 kb on + strand, within GFF4165 at 4187.030 kb on + strand, within GFF4165 at 4187.084 kb on + strand, within GFF4165 at 4187.084 kb on + strand, within GFF4165 at 4187.104 kb on + strand, within GFF4165 at 4187.137 kb on + strand, within GFF4165 at 4187.140 kb on - strand, within GFF4165 at 4187.145 kb on - strand, within GFF4165 at 4187.145 kb on - strand, within GFF4165 at 4187.150 kb on + strand, within GFF4165 at 4187.150 kb on + strand, within GFF4165 at 4187.189 kb on + strand, within GFF4165 at 4187.189 kb on + strand, within GFF4165 at 4187.194 kb on + strand, within GFF4165 at 4187.202 kb on - strand, within GFF4165 at 4187.202 kb on - strand, within GFF4165 at 4187.202 kb on - strand, within GFF4165 at 4187.218 kb on - strand, within GFF4165 at 4187.272 kb on + strand at 4187.308 kb on + strand at 4187.327 kb on + strand at 4187.379 kb on + strand at 4187.379 kb on + strand at 4187.400 kb on + strand at 4187.400 kb on + strand at 4187.402 kb on + strand at 4187.402 kb on + strand at 4187.403 kb on - strand at 4187.410 kb on - strand at 4187.414 kb on + strand at 4187.427 kb on - strand at 4187.431 kb on - strand at 4187.436 kb on - strand at 4187.446 kb on + strand at 4187.451 kb on - strand at 4187.460 kb on + strand at 4187.465 kb on + strand at 4187.478 kb on - strand at 4187.496 kb on + strand, within GFF4166 at 4187.505 kb on + strand, within GFF4166 at 4187.505 kb on + strand, within GFF4166 at 4187.521 kb on + strand, within GFF4166 at 4187.537 kb on - strand, within GFF4166 at 4187.539 kb on + strand, within GFF4166 at 4187.539 kb on + strand, within GFF4166 at 4187.539 kb on + strand, within GFF4166 at 4187.539 kb on + strand, within GFF4166 at 4187.539 kb on + strand, within GFF4166 at 4187.542 kb on - strand, within GFF4166 at 4187.547 kb on - strand, within GFF4166 at 4187.547 kb on - strand, within GFF4166 at 4187.547 kb on - strand, within GFF4166 at 4187.547 kb on - strand, within GFF4166 at 4187.554 kb on - strand, within GFF4166 at 4187.556 kb on + strand, within GFF4166 at 4187.564 kb on - strand, within GFF4166 at 4187.578 kb on - strand, within GFF4166 at 4187.603 kb on + strand, within GFF4166 at 4187.603 kb on + strand, within GFF4166 at 4187.603 kb on + strand, within GFF4166 at 4187.611 kb on - strand, within GFF4166 at 4187.611 kb on - strand, within GFF4166 at 4187.611 kb on - strand, within GFF4166 at 4187.622 kb on + strand, within GFF4166 at 4187.630 kb on - strand, within GFF4166 at 4187.648 kb on + strand, within GFF4166 at 4187.657 kb on + strand, within GFF4166 at 4187.665 kb on - strand, within GFF4166 at 4187.665 kb on - strand, within GFF4166 at 4187.666 kb on - strand, within GFF4166 at 4187.680 kb on + strand, within GFF4166 at 4187.680 kb on + strand, within GFF4166 at 4187.688 kb on + strand, within GFF4166 at 4187.695 kb on - strand, within GFF4166 at 4187.723 kb on + strand, within GFF4166 at 4187.723 kb on + strand, within GFF4166 at 4187.731 kb on - strand, within GFF4166 at 4187.731 kb on - strand, within GFF4166 at 4187.733 kb on + strand, within GFF4166 at 4187.733 kb on - strand, within GFF4166 at 4187.733 kb on - strand, within GFF4166 at 4187.740 kb on + strand, within GFF4166 at 4187.740 kb on + strand, within GFF4166 at 4187.740 kb on + strand, within GFF4166 at 4187.748 kb on - strand, within GFF4166 at 4187.770 kb on - strand, within GFF4166 at 4187.847 kb on - strand, within GFF4166 at 4187.906 kb on - strand, within GFF4166 at 4187.977 kb on + strand at 4187.981 kb on - strand at 4187.994 kb on + strand at 4187.994 kb on + strand at 4188.025 kb on + strand at 4188.039 kb on - strand at 4188.093 kb on + strand at 4188.106 kb on - strand at 4188.117 kb on + strand at 4188.125 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction EMM_noCarbon with Sucrose and Putrescine remove 4,185,841 + +0.1 4,185,849 - -0.2 4,185,861 - -0.4 4,185,913 - +0.1 4,185,918 + +0.1 4,185,929 + -0.2 4,185,929 + -1.5 4,185,929 + -2.0 4,185,935 + +0.2 4,185,937 - -1.1 4,185,937 - -0.6 4,185,980 + -0.4 4,186,009 - +0.5 4,186,018 + +0.5 4,186,024 - -0.6 4,186,026 - -1.9 4,186,060 + -0.4 4,186,084 + GFF4163 0.14 -0.5 4,186,102 + GFF4163 0.19 -0.7 4,186,102 + GFF4163 0.19 +0.0 4,186,118 - GFF4163 0.24 +0.6 4,186,128 - GFF4163 0.27 -1.9 4,186,141 + GFF4163 0.30 -2.1 4,186,141 + GFF4163 0.30 +0.2 4,186,153 + GFF4163 0.34 -1.6 4,186,153 + GFF4163 0.34 +1.0 4,186,159 - GFF4163 0.36 -1.0 4,186,159 - GFF4163 0.36 -0.6 4,186,161 + GFF4163 0.36 -1.8 4,186,161 - GFF4163 0.36 -1.2 4,186,170 + GFF4163 0.39 +0.9 4,186,172 - GFF4163 0.39 -0.3 4,186,173 - GFF4163 0.40 -1.6 4,186,173 - GFF4163 0.40 +0.4 4,186,183 - GFF4163 0.42 -1.1 4,186,188 - GFF4163 0.44 -0.1 4,186,207 - GFF4163 0.49 -0.2 4,186,233 + GFF4163 0.57 -0.9 4,186,245 + GFF4163 0.60 -0.1 4,186,245 + GFF4163 0.60 +0.4 4,186,245 + GFF4163 0.60 -1.2 4,186,253 - GFF4163 0.62 -0.3 4,186,253 - GFF4163 0.62 -0.6 4,186,255 + GFF4163 0.63 -0.9 4,186,255 + GFF4163 0.63 +0.4 4,186,255 + GFF4163 0.63 -0.5 4,186,255 + GFF4163 0.63 +0.6 4,186,255 + GFF4163 0.63 +0.8 4,186,255 + GFF4163 0.63 -1.9 4,186,263 - GFF4163 0.65 +0.8 4,186,263 - GFF4163 0.65 -1.1 4,186,263 - GFF4163 0.65 -2.7 4,186,263 - GFF4163 0.65 +0.2 4,186,278 + GFF4163 0.70 +1.2 4,186,278 + GFF4163 0.70 -0.6 4,186,286 - GFF4163 0.72 -0.1 4,186,286 - GFF4163 0.72 +0.6 4,186,320 + GFF4163 0.81 -0.1 4,186,328 - GFF4163 0.84 -0.2 4,186,330 - GFF4163 0.84 -1.4 4,186,345 - GFF4163 0.89 -0.2 4,186,350 - -0.2 4,186,352 + -1.2 4,186,352 + +0.9 4,186,360 - -0.5 4,186,360 - +1.5 4,186,360 - +0.6 4,186,360 - +1.5 4,186,360 - -0.5 4,186,360 - -0.8 4,186,360 - +0.7 4,186,360 - -0.5 4,186,360 - -0.6 4,186,360 - +2.0 4,186,368 + -0.5 4,186,373 + +0.2 4,186,373 + +0.6 4,186,373 + +1.5 4,186,373 + -0.7 4,186,373 + -1.5 4,186,373 + -1.5 4,186,376 - -2.1 4,186,381 - +0.1 4,186,381 - +1.1 4,186,381 - +0.5 4,186,381 - +0.4 4,186,381 - +0.9 4,186,387 + +0.1 4,186,821 - -2.3 4,186,826 - +0.1 4,186,826 - -0.3 4,186,826 - -0.8 4,186,831 + +0.1 4,186,849 - +0.8 4,186,851 + +0.2 4,186,859 - -0.2 4,186,859 - -0.3 4,186,859 - -2.1 4,186,890 + GFF4165 0.12 +0.4 4,186,890 + GFF4165 0.12 +0.3 4,186,890 + GFF4165 0.12 -0.8 4,186,898 - GFF4165 0.14 -2.7 4,186,898 - GFF4165 0.14 -0.9 4,186,915 - GFF4165 0.17 -0.5 4,186,925 - GFF4165 0.19 -1.3 4,186,943 + GFF4165 0.23 -2.2 4,186,946 - GFF4165 0.24 +0.4 4,186,965 - GFF4165 0.28 -0.6 4,186,973 + GFF4165 0.30 -1.4 4,186,981 - GFF4165 0.31 -0.2 4,186,981 - GFF4165 0.31 +0.1 4,186,993 - GFF4165 0.34 +0.3 4,187,006 + GFF4165 0.37 -1.9 4,187,014 - GFF4165 0.38 -0.3 4,187,030 + GFF4165 0.42 -1.4 4,187,030 + GFF4165 0.42 -2.0 4,187,084 + GFF4165 0.53 -1.5 4,187,084 + GFF4165 0.53 -2.5 4,187,104 + GFF4165 0.58 -1.4 4,187,137 + GFF4165 0.65 -1.8 4,187,140 - GFF4165 0.65 -1.7 4,187,145 - GFF4165 0.66 -1.5 4,187,145 - GFF4165 0.66 +0.2 4,187,150 + GFF4165 0.68 -1.4 4,187,150 + GFF4165 0.68 -0.2 4,187,189 + GFF4165 0.76 +0.2 4,187,189 + GFF4165 0.76 -1.0 4,187,194 + GFF4165 0.77 -1.0 4,187,202 - GFF4165 0.79 -2.6 4,187,202 - GFF4165 0.79 -0.6 4,187,202 - GFF4165 0.79 -0.5 4,187,218 - GFF4165 0.82 -1.5 4,187,272 + -0.4 4,187,308 + -1.4 4,187,327 + +0.3 4,187,379 + -3.4 4,187,379 + -0.8 4,187,400 + -1.2 4,187,400 + +0.4 4,187,402 + -0.3 4,187,402 + +0.5 4,187,403 - +0.4 4,187,410 - +0.3 4,187,414 + -1.2 4,187,427 - +0.6 4,187,431 - +0.2 4,187,436 - -1.4 4,187,446 + -1.9 4,187,451 - -0.2 4,187,460 + +0.5 4,187,465 + +0.0 4,187,478 - -1.5 4,187,496 + GFF4166 0.11 -2.0 4,187,505 + GFF4166 0.12 +0.2 4,187,505 + GFF4166 0.12 -1.2 4,187,521 + GFF4166 0.15 -0.6 4,187,537 - GFF4166 0.18 -0.7 4,187,539 + GFF4166 0.18 -0.1 4,187,539 + GFF4166 0.18 +0.3 4,187,539 + GFF4166 0.18 -0.2 4,187,539 + GFF4166 0.18 +0.3 4,187,539 + GFF4166 0.18 -0.1 4,187,542 - GFF4166 0.18 -1.4 4,187,547 - GFF4166 0.19 -0.5 4,187,547 - GFF4166 0.19 +1.4 4,187,547 - GFF4166 0.19 -0.1 4,187,547 - GFF4166 0.19 +1.4 4,187,554 - GFF4166 0.20 -0.3 4,187,556 + GFF4166 0.21 -0.7 4,187,564 - GFF4166 0.22 -0.7 4,187,578 - GFF4166 0.24 +1.0 4,187,603 + GFF4166 0.29 -0.1 4,187,603 + GFF4166 0.29 +1.1 4,187,603 + GFF4166 0.29 +0.7 4,187,611 - GFF4166 0.30 +0.6 4,187,611 - GFF4166 0.30 -0.5 4,187,611 - GFF4166 0.30 -0.3 4,187,622 + GFF4166 0.32 -0.6 4,187,630 - GFF4166 0.33 +0.7 4,187,648 + GFF4166 0.36 -0.1 4,187,657 + GFF4166 0.38 -0.8 4,187,665 - GFF4166 0.39 +0.5 4,187,665 - GFF4166 0.39 +0.6 4,187,666 - GFF4166 0.39 +0.4 4,187,680 + GFF4166 0.42 +1.2 4,187,680 + GFF4166 0.42 -0.9 4,187,688 + GFF4166 0.43 +1.3 4,187,695 - GFF4166 0.44 -0.4 4,187,723 + GFF4166 0.49 -0.3 4,187,723 + GFF4166 0.49 -0.8 4,187,731 - GFF4166 0.50 -0.4 4,187,731 - GFF4166 0.50 +0.2 4,187,733 + GFF4166 0.50 +2.0 4,187,733 - GFF4166 0.50 +1.6 4,187,733 - GFF4166 0.50 -1.4 4,187,740 + GFF4166 0.52 -0.0 4,187,740 + GFF4166 0.52 +0.3 4,187,740 + GFF4166 0.52 +1.7 4,187,748 - GFF4166 0.53 -0.8 4,187,770 - GFF4166 0.57 -0.2 4,187,847 - GFF4166 0.70 -2.0 4,187,906 - GFF4166 0.79 +0.3 4,187,977 + -2.0 4,187,981 - -0.3 4,187,994 + -0.6 4,187,994 + -1.0 4,188,025 + -1.7 4,188,039 - -0.5 4,188,093 + -0.6 4,188,106 - -1.4 4,188,117 + -0.2 4,188,125 - +0.8
Or see this region's nucleotide sequence