Experiment: EMM_noCarbon with Sucrose and Putrescine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF375 and GFF376 overlap by 4 nucleotides GFF376 and GFF377 are separated by 7 nucleotides GFF377 and GFF378 are separated by 27 nucleotides GFF378 and GFF379 are separated by 20 nucleotides GFF379 and GFF380 overlap by 4 nucleotides
GFF375 - Peptidoglycan lipid II flippase MurJ, at 367,732 to 369,306
GFF375
GFF376 - hypothetical protein, at 369,303 to 369,422
GFF376
GFF377 - Protein-export protein SecB (maintains pre-export unfolded state), at 369,430 to 369,933
GFF377
GFF378 - hypothetical protein, at 369,961 to 370,092
GFF378
GFF379 - Tim44-like domain protein, at 370,113 to 370,811
GFF379
GFF380 - Membrane-bound lytic murein transglycosylase A, at 370,808 to 372,073
GFF380
Position (kb)
370
371 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 369.308 kb on - strand at 369.326 kb on + strand, within GFF376 at 369.333 kb on + strand, within GFF376 at 369.339 kb on + strand, within GFF376 at 369.339 kb on + strand, within GFF376 at 369.339 kb on + strand, within GFF376 at 369.339 kb on + strand, within GFF376 at 369.339 kb on + strand, within GFF376 at 369.339 kb on + strand, within GFF376 at 369.339 kb on + strand, within GFF376 at 369.339 kb on + strand, within GFF376 at 369.339 kb on + strand, within GFF376 at 369.339 kb on + strand, within GFF376 at 369.339 kb on + strand, within GFF376 at 369.344 kb on + strand, within GFF376 at 369.344 kb on + strand, within GFF376 at 369.344 kb on + strand, within GFF376 at 369.344 kb on + strand, within GFF376 at 369.344 kb on + strand, within GFF376 at 369.347 kb on - strand, within GFF376 at 369.347 kb on - strand, within GFF376 at 369.347 kb on - strand, within GFF376 at 369.347 kb on - strand, within GFF376 at 369.347 kb on - strand, within GFF376 at 369.347 kb on - strand, within GFF376 at 369.347 kb on - strand, within GFF376 at 369.347 kb on - strand, within GFF376 at 369.347 kb on - strand, within GFF376 at 369.347 kb on - strand, within GFF376 at 369.347 kb on - strand, within GFF376 at 369.347 kb on - strand, within GFF376 at 369.347 kb on - strand, within GFF376 at 369.347 kb on - strand, within GFF376 at 369.347 kb on - strand, within GFF376 at 369.352 kb on - strand, within GFF376 at 369.352 kb on - strand, within GFF376 at 369.352 kb on - strand, within GFF376 at 369.352 kb on - strand, within GFF376 at 369.352 kb on - strand, within GFF376 at 369.352 kb on - strand, within GFF376 at 369.354 kb on - strand, within GFF376 at 369.362 kb on + strand, within GFF376 at 369.377 kb on - strand, within GFF376 at 369.385 kb on + strand, within GFF376 at 369.385 kb on + strand, within GFF376 at 369.393 kb on - strand, within GFF376 at 369.393 kb on - strand, within GFF376 at 369.395 kb on + strand, within GFF376 at 369.395 kb on + strand, within GFF376 at 369.395 kb on + strand, within GFF376 at 369.395 kb on + strand, within GFF376 at 369.395 kb on + strand, within GFF376 at 369.395 kb on + strand, within GFF376 at 369.403 kb on - strand, within GFF376 at 369.415 kb on + strand at 369.436 kb on - strand at 369.436 kb on - strand at 369.439 kb on + strand at 369.458 kb on - strand at 369.460 kb on + strand at 369.471 kb on + strand at 369.479 kb on - strand at 369.479 kb on - strand at 369.480 kb on + strand at 369.480 kb on + strand at 369.480 kb on + strand at 369.480 kb on + strand at 369.480 kb on + strand at 369.480 kb on + strand at 369.488 kb on - strand, within GFF377 at 369.488 kb on - strand, within GFF377 at 369.488 kb on - strand, within GFF377 at 369.503 kb on - strand, within GFF377 at 369.522 kb on - strand, within GFF377 at 369.561 kb on - strand, within GFF377 at 369.561 kb on - strand, within GFF377 at 369.561 kb on - strand, within GFF377 at 369.561 kb on - strand, within GFF377 at 369.574 kb on + strand, within GFF377 at 369.603 kb on + strand, within GFF377 at 369.610 kb on + strand, within GFF377 at 369.611 kb on - strand, within GFF377 at 369.817 kb on - strand, within GFF377 at 369.945 kb on - strand at 369.945 kb on - strand at 369.945 kb on - strand at 369.945 kb on - strand at 369.952 kb on - strand at 369.979 kb on - strand, within GFF378 at 369.979 kb on - strand, within GFF378 at 369.979 kb on - strand, within GFF378 at 369.988 kb on + strand, within GFF378 at 369.996 kb on - strand, within GFF378 at 370.024 kb on + strand, within GFF378 at 370.024 kb on + strand, within GFF378 at 370.032 kb on - strand, within GFF378 at 370.032 kb on - strand, within GFF378 at 370.039 kb on + strand, within GFF378 at 370.039 kb on + strand, within GFF378 at 370.065 kb on + strand, within GFF378 at 370.070 kb on + strand, within GFF378 at 370.070 kb on + strand, within GFF378 at 370.078 kb on - strand, within GFF378 at 370.078 kb on - strand, within GFF378 at 370.078 kb on - strand, within GFF378 at 370.078 kb on - strand, within GFF378 at 370.096 kb on + strand at 370.108 kb on + strand at 370.141 kb on + strand at 370.143 kb on + strand at 370.143 kb on + strand at 370.143 kb on + strand at 370.143 kb on + strand at 370.143 kb on + strand at 370.173 kb on + strand at 370.218 kb on - strand, within GFF379 at 370.241 kb on + strand, within GFF379 at 370.244 kb on + strand, within GFF379 at 370.288 kb on + strand, within GFF379 at 370.303 kb on - strand, within GFF379 at 370.376 kb on - strand, within GFF379 at 370.385 kb on + strand, within GFF379 at 370.385 kb on + strand, within GFF379 at 370.393 kb on - strand, within GFF379 at 370.393 kb on - strand, within GFF379 at 370.485 kb on + strand, within GFF379 at 370.493 kb on - strand, within GFF379 at 370.504 kb on + strand, within GFF379 at 370.507 kb on - strand, within GFF379 at 370.512 kb on + strand, within GFF379 at 370.514 kb on + strand, within GFF379 at 370.557 kb on + strand, within GFF379 at 370.737 kb on + strand, within GFF379 at 370.770 kb on + strand at 370.806 kb on + strand at 370.806 kb on + strand at 370.806 kb on + strand at 370.806 kb on + strand at 370.814 kb on - strand at 370.814 kb on - strand at 370.814 kb on - strand at 370.814 kb on - strand at 370.872 kb on + strand at 370.896 kb on + strand at 370.904 kb on - strand at 370.909 kb on - strand at 370.946 kb on + strand, within GFF380 at 370.946 kb on + strand, within GFF380 at 370.962 kb on + strand, within GFF380 at 370.962 kb on + strand, within GFF380 at 370.970 kb on - strand, within GFF380 at 370.970 kb on - strand, within GFF380 at 370.970 kb on - strand, within GFF380 at 370.972 kb on + strand, within GFF380 at 371.013 kb on - strand, within GFF380 at 371.015 kb on + strand, within GFF380 at 371.015 kb on + strand, within GFF380 at 371.015 kb on + strand, within GFF380 at 371.020 kb on + strand, within GFF380 at 371.023 kb on - strand, within GFF380 at 371.028 kb on + strand, within GFF380 at 371.075 kb on - strand, within GFF380 at 371.076 kb on + strand, within GFF380 at 371.084 kb on - strand, within GFF380 at 371.102 kb on + strand, within GFF380 at 371.147 kb on + strand, within GFF380 at 371.147 kb on + strand, within GFF380 at 371.147 kb on + strand, within GFF380 at 371.150 kb on - strand, within GFF380 at 371.150 kb on - strand, within GFF380 at 371.150 kb on - strand, within GFF380 at 371.155 kb on - strand, within GFF380 at 371.155 kb on - strand, within GFF380 at 371.169 kb on + strand, within GFF380 at 371.174 kb on + strand, within GFF380 at 371.174 kb on + strand, within GFF380 at 371.174 kb on + strand, within GFF380 at 371.174 kb on + strand, within GFF380 at 371.177 kb on - strand, within GFF380 at 371.182 kb on - strand, within GFF380 at 371.182 kb on - strand, within GFF380 at 371.182 kb on - strand, within GFF380 at 371.182 kb on - strand, within GFF380 at 371.182 kb on - strand, within GFF380 at 371.182 kb on - strand, within GFF380 at 371.198 kb on + strand, within GFF380 at 371.206 kb on - strand, within GFF380 at 371.206 kb on - strand, within GFF380 at 371.244 kb on + strand, within GFF380 at 371.255 kb on + strand, within GFF380 at 371.263 kb on - strand, within GFF380 at 371.263 kb on - strand, within GFF380 at 371.263 kb on - strand, within GFF380 at 371.269 kb on + strand, within GFF380 at 371.269 kb on + strand, within GFF380 at 371.269 kb on + strand, within GFF380 at 371.269 kb on + strand, within GFF380 at 371.272 kb on - strand, within GFF380 at 371.284 kb on - strand, within GFF380 at 371.289 kb on + strand, within GFF380 at 371.334 kb on + strand, within GFF380 at 371.334 kb on + strand, within GFF380 at 371.366 kb on + strand, within GFF380 at 371.382 kb on + strand, within GFF380 at 371.390 kb on - strand, within GFF380 at 371.390 kb on - strand, within GFF380 at 371.400 kb on - strand, within GFF380 at 371.400 kb on - strand, within GFF380 at 371.403 kb on + strand, within GFF380 at 371.403 kb on + strand, within GFF380 at 371.411 kb on + strand, within GFF380 at 371.411 kb on - strand, within GFF380 at 371.412 kb on - strand, within GFF380 at 371.432 kb on + strand, within GFF380 at 371.432 kb on + strand, within GFF380 at 371.438 kb on - strand, within GFF380 at 371.440 kb on - strand, within GFF380 at 371.440 kb on - strand, within GFF380 at 371.440 kb on - strand, within GFF380 at 371.440 kb on - strand, within GFF380 at 371.440 kb on - strand, within GFF380 at 371.502 kb on + strand, within GFF380 at 371.502 kb on + strand, within GFF380 at 371.507 kb on + strand, within GFF380 at 371.507 kb on + strand, within GFF380 at 371.510 kb on - strand, within GFF380 at 371.510 kb on - strand, within GFF380 at 371.510 kb on - strand, within GFF380 at 371.512 kb on + strand, within GFF380 at 371.515 kb on - strand, within GFF380 at 371.520 kb on - strand, within GFF380 at 371.546 kb on + strand, within GFF380 at 371.551 kb on + strand, within GFF380 at 371.552 kb on + strand, within GFF380 at 371.552 kb on + strand, within GFF380 at 371.554 kb on + strand, within GFF380 at 371.560 kb on - strand, within GFF380 at 371.560 kb on - strand, within GFF380 at 371.565 kb on - strand, within GFF380 at 371.565 kb on - strand, within GFF380 at 371.565 kb on - strand, within GFF380 at 371.567 kb on + strand, within GFF380 at 371.568 kb on + strand, within GFF380 at 371.568 kb on + strand, within GFF380 at 371.568 kb on + strand, within GFF380 at 371.568 kb on + strand, within GFF380 at 371.569 kb on + strand, within GFF380 at 371.576 kb on - strand, within GFF380 at 371.576 kb on - strand, within GFF380 at 371.604 kb on + strand, within GFF380 at 371.607 kb on + strand, within GFF380 at 371.613 kb on + strand, within GFF380 at 371.615 kb on - strand, within GFF380 at 371.639 kb on - strand, within GFF380 at 371.668 kb on + strand, within GFF380 at 371.668 kb on + strand, within GFF380 at 371.668 kb on + strand, within GFF380 at 371.668 kb on + strand, within GFF380 at 371.668 kb on + strand, within GFF380 at 371.672 kb on - strand, within GFF380 at 371.674 kb on + strand, within GFF380 at 371.676 kb on - strand, within GFF380 at 371.687 kb on - strand, within GFF380 at 371.692 kb on - strand, within GFF380 at 371.692 kb on - strand, within GFF380 at 371.723 kb on - strand, within GFF380 at 371.754 kb on + strand, within GFF380 at 371.781 kb on + strand, within GFF380 at 371.781 kb on + strand, within GFF380 at 371.789 kb on - strand, within GFF380 at 371.810 kb on + strand, within GFF380
Per-strain Table
Position Strand Gene LocusTag Fraction EMM_noCarbon with Sucrose and Putrescine remove 369,308 - -0.3 369,326 + GFF376 0.19 +0.6 369,333 + GFF376 0.25 -0.2 369,339 + GFF376 0.30 -1.6 369,339 + GFF376 0.30 +0.1 369,339 + GFF376 0.30 -0.4 369,339 + GFF376 0.30 +0.8 369,339 + GFF376 0.30 -0.3 369,339 + GFF376 0.30 +0.4 369,339 + GFF376 0.30 +0.9 369,339 + GFF376 0.30 +0.3 369,339 + GFF376 0.30 +0.5 369,339 + GFF376 0.30 -1.1 369,339 + GFF376 0.30 -0.6 369,344 + GFF376 0.34 -2.3 369,344 + GFF376 0.34 -0.0 369,344 + GFF376 0.34 -0.6 369,344 + GFF376 0.34 +1.1 369,344 + GFF376 0.34 +0.8 369,347 - GFF376 0.37 -0.0 369,347 - GFF376 0.37 -1.1 369,347 - GFF376 0.37 +0.2 369,347 - GFF376 0.37 +0.4 369,347 - GFF376 0.37 +0.9 369,347 - GFF376 0.37 -0.3 369,347 - GFF376 0.37 -1.7 369,347 - GFF376 0.37 -0.4 369,347 - GFF376 0.37 -2.9 369,347 - GFF376 0.37 -0.3 369,347 - GFF376 0.37 -0.6 369,347 - GFF376 0.37 -0.6 369,347 - GFF376 0.37 -0.3 369,347 - GFF376 0.37 -0.4 369,347 - GFF376 0.37 -0.8 369,352 - GFF376 0.41 +0.2 369,352 - GFF376 0.41 -0.8 369,352 - GFF376 0.41 -1.7 369,352 - GFF376 0.41 +0.7 369,352 - GFF376 0.41 -1.4 369,352 - GFF376 0.41 -0.3 369,354 - GFF376 0.42 -1.2 369,362 + GFF376 0.49 -0.8 369,377 - GFF376 0.62 -0.1 369,385 + GFF376 0.68 -1.6 369,385 + GFF376 0.68 -1.3 369,393 - GFF376 0.75 +0.4 369,393 - GFF376 0.75 -1.7 369,395 + GFF376 0.77 -0.1 369,395 + GFF376 0.77 +0.1 369,395 + GFF376 0.77 +0.5 369,395 + GFF376 0.77 -0.9 369,395 + GFF376 0.77 -0.1 369,395 + GFF376 0.77 +0.5 369,403 - GFF376 0.83 -0.3 369,415 + -0.2 369,436 - +0.8 369,436 - -0.4 369,439 + +0.2 369,458 - +0.5 369,460 + +0.0 369,471 + -0.2 369,479 - +0.7 369,479 - -0.0 369,480 + -1.2 369,480 + +0.9 369,480 + -2.1 369,480 + +0.4 369,480 + +0.7 369,480 + -1.6 369,488 - GFF377 0.12 +0.9 369,488 - GFF377 0.12 +1.3 369,488 - GFF377 0.12 -0.5 369,503 - GFF377 0.14 -0.6 369,522 - GFF377 0.18 +1.3 369,561 - GFF377 0.26 -1.4 369,561 - GFF377 0.26 -0.5 369,561 - GFF377 0.26 -0.3 369,561 - GFF377 0.26 -0.4 369,574 + GFF377 0.29 -1.2 369,603 + GFF377 0.34 +1.2 369,610 + GFF377 0.36 -1.7 369,611 - GFF377 0.36 +0.7 369,817 - GFF377 0.77 -1.9 369,945 - +0.7 369,945 - -0.9 369,945 - +1.4 369,945 - -2.3 369,952 - +0.9 369,979 - GFF378 0.14 -0.3 369,979 - GFF378 0.14 -0.0 369,979 - GFF378 0.14 -2.3 369,988 + GFF378 0.20 -0.0 369,996 - GFF378 0.27 -0.9 370,024 + GFF378 0.48 +0.0 370,024 + GFF378 0.48 -1.3 370,032 - GFF378 0.54 -0.3 370,032 - GFF378 0.54 -0.4 370,039 + GFF378 0.59 +0.7 370,039 + GFF378 0.59 -0.9 370,065 + GFF378 0.79 +0.6 370,070 + GFF378 0.83 +0.3 370,070 + GFF378 0.83 -1.2 370,078 - GFF378 0.89 -0.3 370,078 - GFF378 0.89 -0.8 370,078 - GFF378 0.89 -1.6 370,078 - GFF378 0.89 -0.4 370,096 + -1.1 370,108 + -1.3 370,141 + -0.2 370,143 + -0.8 370,143 + -1.4 370,143 + +0.7 370,143 + +0.2 370,143 + +1.2 370,173 + -1.5 370,218 - GFF379 0.15 -0.4 370,241 + GFF379 0.18 +0.5 370,244 + GFF379 0.19 -0.8 370,288 + GFF379 0.25 -0.6 370,303 - GFF379 0.27 -1.6 370,376 - GFF379 0.38 -1.3 370,385 + GFF379 0.39 -1.5 370,385 + GFF379 0.39 +1.3 370,393 - GFF379 0.40 -1.5 370,393 - GFF379 0.40 -1.8 370,485 + GFF379 0.53 -1.3 370,493 - GFF379 0.54 -1.3 370,504 + GFF379 0.56 -1.4 370,507 - GFF379 0.56 -1.2 370,512 + GFF379 0.57 -1.8 370,514 + GFF379 0.57 -1.9 370,557 + GFF379 0.64 -0.0 370,737 + GFF379 0.89 -1.8 370,770 + +1.4 370,806 + +0.2 370,806 + -1.0 370,806 + -1.2 370,806 + -0.0 370,814 - +0.1 370,814 - -1.5 370,814 - +0.6 370,814 - +0.5 370,872 + -1.3 370,896 + -0.7 370,904 - -0.3 370,909 - +0.7 370,946 + GFF380 0.11 +0.4 370,946 + GFF380 0.11 +1.0 370,962 + GFF380 0.12 +1.2 370,962 + GFF380 0.12 -1.6 370,970 - GFF380 0.13 +1.5 370,970 - GFF380 0.13 -0.8 370,970 - GFF380 0.13 -0.5 370,972 + GFF380 0.13 -2.2 371,013 - GFF380 0.16 +1.3 371,015 + GFF380 0.16 -1.3 371,015 + GFF380 0.16 -1.3 371,015 + GFF380 0.16 -0.7 371,020 + GFF380 0.17 +0.6 371,023 - GFF380 0.17 -1.0 371,028 + GFF380 0.17 -0.5 371,075 - GFF380 0.21 -0.7 371,076 + GFF380 0.21 -1.3 371,084 - GFF380 0.22 -0.3 371,102 + GFF380 0.23 -1.3 371,147 + GFF380 0.27 +0.8 371,147 + GFF380 0.27 -1.3 371,147 + GFF380 0.27 -1.9 371,150 - GFF380 0.27 +0.5 371,150 - GFF380 0.27 -0.4 371,150 - GFF380 0.27 -0.3 371,155 - GFF380 0.27 -0.6 371,155 - GFF380 0.27 -0.2 371,169 + GFF380 0.29 +1.2 371,174 + GFF380 0.29 +0.4 371,174 + GFF380 0.29 +0.8 371,174 + GFF380 0.29 +0.1 371,174 + GFF380 0.29 -0.1 371,177 - GFF380 0.29 -1.7 371,182 - GFF380 0.30 -0.8 371,182 - GFF380 0.30 -1.3 371,182 - GFF380 0.30 -0.3 371,182 - GFF380 0.30 -0.0 371,182 - GFF380 0.30 -3.3 371,182 - GFF380 0.30 +0.1 371,198 + GFF380 0.31 -0.9 371,206 - GFF380 0.31 -1.9 371,206 - GFF380 0.31 -0.5 371,244 + GFF380 0.34 -1.4 371,255 + GFF380 0.35 -1.0 371,263 - GFF380 0.36 +0.7 371,263 - GFF380 0.36 -1.7 371,263 - GFF380 0.36 -1.2 371,269 + GFF380 0.36 -1.9 371,269 + GFF380 0.36 -0.3 371,269 + GFF380 0.36 -1.4 371,269 + GFF380 0.36 +0.6 371,272 - GFF380 0.37 +0.6 371,284 - GFF380 0.38 -1.1 371,289 + GFF380 0.38 -0.2 371,334 + GFF380 0.42 -0.2 371,334 + GFF380 0.42 -0.2 371,366 + GFF380 0.44 +1.0 371,382 + GFF380 0.45 -0.3 371,390 - GFF380 0.46 +0.2 371,390 - GFF380 0.46 +0.5 371,400 - GFF380 0.47 +0.0 371,400 - GFF380 0.47 -0.5 371,403 + GFF380 0.47 -1.6 371,403 + GFF380 0.47 +0.2 371,411 + GFF380 0.48 -1.0 371,411 - GFF380 0.48 +0.6 371,412 - GFF380 0.48 +0.8 371,432 + GFF380 0.49 -0.2 371,432 + GFF380 0.49 -2.0 371,438 - GFF380 0.50 +0.4 371,440 - GFF380 0.50 +1.7 371,440 - GFF380 0.50 -0.8 371,440 - GFF380 0.50 -0.4 371,440 - GFF380 0.50 +0.7 371,440 - GFF380 0.50 +0.1 371,502 + GFF380 0.55 -0.6 371,502 + GFF380 0.55 -0.0 371,507 + GFF380 0.55 +0.0 371,507 + GFF380 0.55 -0.5 371,510 - GFF380 0.55 -0.9 371,510 - GFF380 0.55 -0.1 371,510 - GFF380 0.55 -0.6 371,512 + GFF380 0.56 -0.1 371,515 - GFF380 0.56 +0.4 371,520 - GFF380 0.56 +0.5 371,546 + GFF380 0.58 +0.7 371,551 + GFF380 0.59 +0.0 371,552 + GFF380 0.59 +0.6 371,552 + GFF380 0.59 -0.3 371,554 + GFF380 0.59 +0.8 371,560 - GFF380 0.59 +0.1 371,560 - GFF380 0.59 -1.2 371,565 - GFF380 0.60 +0.8 371,565 - GFF380 0.60 -0.7 371,565 - GFF380 0.60 -0.2 371,567 + GFF380 0.60 -0.2 371,568 + GFF380 0.60 +0.9 371,568 + GFF380 0.60 -1.9 371,568 + GFF380 0.60 +0.4 371,568 + GFF380 0.60 -0.2 371,569 + GFF380 0.60 -0.7 371,576 - GFF380 0.61 -1.4 371,576 - GFF380 0.61 -0.5 371,604 + GFF380 0.63 +0.6 371,607 + GFF380 0.63 -0.3 371,613 + GFF380 0.64 -0.5 371,615 - GFF380 0.64 +0.1 371,639 - GFF380 0.66 +0.2 371,668 + GFF380 0.68 +0.9 371,668 + GFF380 0.68 -1.0 371,668 + GFF380 0.68 -0.5 371,668 + GFF380 0.68 -0.4 371,668 + GFF380 0.68 -1.8 371,672 - GFF380 0.68 +0.2 371,674 + GFF380 0.68 +1.3 371,676 - GFF380 0.69 -1.0 371,687 - GFF380 0.69 -2.0 371,692 - GFF380 0.70 -0.5 371,692 - GFF380 0.70 +0.2 371,723 - GFF380 0.72 -0.1 371,754 + GFF380 0.75 +1.3 371,781 + GFF380 0.77 -1.2 371,781 + GFF380 0.77 -1.4 371,789 - GFF380 0.77 +1.2 371,810 + GFF380 0.79 -1.2
Or see this region's nucleotide sequence