Experiment: EMM_noCarbon with Sucrose and Putrescine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF357 and GFF358 are separated by 83 nucleotides GFF358 and GFF359 are separated by 12 nucleotides GFF359 and GFF360 overlap by 4 nucleotides GFF360 and GFF361 are separated by 102 nucleotides
GFF357 - 3-dehydroquinate dehydratase II (EC 4.2.1.10), at 349,296 to 349,736
GFF357
GFF358 - Biotin carboxyl carrier protein of acetyl-CoA carboxylase, at 349,820 to 350,308
GFF358
GFF359 - Biotin carboxylase of acetyl-CoA carboxylase (EC 6.3.4.14), at 350,321 to 351,673
GFF359
GFF360 - Uncharacterized protein YfgD, not an arsenate reductase, at 351,670 to 352,014
GFF360
GFF361 - ParA-like protein, at 352,117 to 352,848
GFF361
Position (kb)
350
351
352 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 349.338 kb on - strand at 349.608 kb on - strand, within GFF357 at 349.678 kb on - strand, within GFF357 at 349.722 kb on + strand at 349.730 kb on - strand at 349.730 kb on - strand at 349.730 kb on - strand at 349.731 kb on + strand at 349.731 kb on + strand at 349.731 kb on - strand at 349.733 kb on + strand at 349.748 kb on - strand at 351.636 kb on + strand at 351.668 kb on - strand at 351.678 kb on - strand at 351.678 kb on - strand at 351.684 kb on - strand at 351.694 kb on + strand at 351.702 kb on - strand at 351.719 kb on - strand, within GFF360 at 351.746 kb on + strand, within GFF360 at 351.752 kb on + strand, within GFF360 at 351.753 kb on + strand, within GFF360 at 351.760 kb on - strand, within GFF360 at 351.771 kb on + strand, within GFF360 at 351.780 kb on - strand, within GFF360 at 351.800 kb on - strand, within GFF360 at 351.820 kb on + strand, within GFF360 at 351.824 kb on + strand, within GFF360 at 351.852 kb on + strand, within GFF360 at 351.863 kb on + strand, within GFF360 at 351.868 kb on + strand, within GFF360 at 351.871 kb on - strand, within GFF360 at 351.880 kb on + strand, within GFF360 at 351.899 kb on + strand, within GFF360 at 351.901 kb on - strand, within GFF360 at 351.942 kb on - strand, within GFF360 at 351.990 kb on + strand at 351.990 kb on + strand at 351.990 kb on + strand at 351.998 kb on - strand at 351.998 kb on - strand at 351.998 kb on - strand at 351.998 kb on - strand at 351.998 kb on - strand at 352.073 kb on - strand at 352.082 kb on - strand at 352.114 kb on + strand at 352.117 kb on - strand at 352.139 kb on + strand at 352.147 kb on - strand at 352.147 kb on - strand at 352.185 kb on - strand at 352.202 kb on - strand, within GFF361 at 352.210 kb on - strand, within GFF361 at 352.210 kb on - strand, within GFF361 at 352.221 kb on + strand, within GFF361 at 352.221 kb on + strand, within GFF361 at 352.221 kb on + strand, within GFF361 at 352.221 kb on + strand, within GFF361 at 352.229 kb on - strand, within GFF361 at 352.229 kb on - strand, within GFF361 at 352.229 kb on - strand, within GFF361 at 352.231 kb on + strand, within GFF361 at 352.246 kb on + strand, within GFF361 at 352.252 kb on + strand, within GFF361 at 352.260 kb on + strand, within GFF361 at 352.260 kb on + strand, within GFF361 at 352.268 kb on - strand, within GFF361 at 352.268 kb on - strand, within GFF361 at 352.273 kb on - strand, within GFF361 at 352.273 kb on - strand, within GFF361 at 352.288 kb on - strand, within GFF361 at 352.291 kb on + strand, within GFF361 at 352.291 kb on + strand, within GFF361 at 352.291 kb on + strand, within GFF361 at 352.296 kb on + strand, within GFF361 at 352.298 kb on - strand, within GFF361 at 352.299 kb on - strand, within GFF361 at 352.321 kb on - strand, within GFF361 at 352.338 kb on + strand, within GFF361 at 352.338 kb on + strand, within GFF361 at 352.338 kb on + strand, within GFF361 at 352.338 kb on + strand, within GFF361 at 352.338 kb on + strand, within GFF361 at 352.338 kb on + strand, within GFF361 at 352.338 kb on + strand, within GFF361 at 352.338 kb on + strand, within GFF361 at 352.340 kb on - strand, within GFF361 at 352.341 kb on - strand, within GFF361 at 352.343 kb on + strand, within GFF361 at 352.346 kb on - strand, within GFF361 at 352.346 kb on - strand, within GFF361 at 352.349 kb on + strand, within GFF361 at 352.354 kb on + strand, within GFF361 at 352.354 kb on + strand, within GFF361 at 352.355 kb on + strand, within GFF361 at 352.355 kb on + strand, within GFF361 at 352.355 kb on + strand, within GFF361 at 352.355 kb on + strand, within GFF361 at 352.355 kb on + strand, within GFF361 at 352.359 kb on + strand, within GFF361 at 352.362 kb on - strand, within GFF361 at 352.363 kb on - strand, within GFF361 at 352.363 kb on - strand, within GFF361 at 352.363 kb on - strand, within GFF361 at 352.363 kb on - strand, within GFF361 at 352.365 kb on + strand, within GFF361 at 352.386 kb on + strand, within GFF361 at 352.386 kb on + strand, within GFF361 at 352.386 kb on + strand, within GFF361 at 352.391 kb on + strand, within GFF361 at 352.399 kb on - strand, within GFF361 at 352.399 kb on - strand, within GFF361 at 352.434 kb on + strand, within GFF361 at 352.462 kb on + strand, within GFF361 at 352.462 kb on + strand, within GFF361 at 352.463 kb on - strand, within GFF361 at 352.470 kb on - strand, within GFF361 at 352.476 kb on - strand, within GFF361 at 352.498 kb on - strand, within GFF361 at 352.501 kb on + strand, within GFF361 at 352.519 kb on + strand, within GFF361 at 352.522 kb on - strand, within GFF361 at 352.529 kb on + strand, within GFF361 at 352.529 kb on + strand, within GFF361 at 352.537 kb on - strand, within GFF361 at 352.537 kb on - strand, within GFF361 at 352.537 kb on - strand, within GFF361 at 352.537 kb on - strand, within GFF361 at 352.556 kb on + strand, within GFF361 at 352.562 kb on - strand, within GFF361 at 352.584 kb on + strand, within GFF361 at 352.592 kb on - strand, within GFF361 at 352.592 kb on - strand, within GFF361 at 352.599 kb on + strand, within GFF361 at 352.601 kb on + strand, within GFF361 at 352.601 kb on + strand, within GFF361 at 352.601 kb on + strand, within GFF361 at 352.601 kb on + strand, within GFF361 at 352.601 kb on + strand, within GFF361 at 352.601 kb on + strand, within GFF361 at 352.601 kb on + strand, within GFF361 at 352.601 kb on + strand, within GFF361 at 352.609 kb on - strand, within GFF361 at 352.609 kb on - strand, within GFF361 at 352.609 kb on - strand, within GFF361 at 352.609 kb on - strand, within GFF361 at 352.609 kb on - strand, within GFF361 at 352.609 kb on - strand, within GFF361 at 352.609 kb on - strand, within GFF361 at 352.609 kb on - strand, within GFF361 at 352.609 kb on - strand, within GFF361 at 352.614 kb on - strand, within GFF361 at 352.636 kb on - strand, within GFF361 at 352.646 kb on + strand, within GFF361 at 352.662 kb on - strand, within GFF361
Per-strain Table
Position Strand Gene LocusTag Fraction EMM_noCarbon with Sucrose and Putrescine remove 349,338 - -1.3 349,608 - GFF357 0.71 -1.4 349,678 - GFF357 0.87 -1.2 349,722 + +0.9 349,730 - -1.3 349,730 - +0.1 349,730 - -0.8 349,731 + -1.1 349,731 + -0.2 349,731 - -1.2 349,733 + +0.8 349,748 - +1.2 351,636 + +1.2 351,668 - -2.4 351,678 - -0.7 351,678 - +0.9 351,684 - +0.5 351,694 + +1.1 351,702 - +0.1 351,719 - GFF360 0.14 -2.6 351,746 + GFF360 0.22 -1.3 351,752 + GFF360 0.24 -0.5 351,753 + GFF360 0.24 -0.6 351,760 - GFF360 0.26 -2.4 351,771 + GFF360 0.29 +0.7 351,780 - GFF360 0.32 -1.0 351,800 - GFF360 0.38 +0.5 351,820 + GFF360 0.43 +0.4 351,824 + GFF360 0.45 -1.9 351,852 + GFF360 0.53 -0.0 351,863 + GFF360 0.56 -0.9 351,868 + GFF360 0.57 +0.3 351,871 - GFF360 0.58 -0.3 351,880 + GFF360 0.61 +0.7 351,899 + GFF360 0.66 -2.0 351,901 - GFF360 0.67 -1.6 351,942 - GFF360 0.79 -0.4 351,990 + +0.6 351,990 + -2.0 351,990 + -1.0 351,998 - -0.1 351,998 - +0.1 351,998 - +0.3 351,998 - -0.5 351,998 - +1.5 352,073 - +0.8 352,082 - -0.6 352,114 + -1.7 352,117 - +0.7 352,139 + -0.1 352,147 - +0.6 352,147 - -2.3 352,185 - -2.3 352,202 - GFF361 0.12 -3.0 352,210 - GFF361 0.13 +0.1 352,210 - GFF361 0.13 +0.9 352,221 + GFF361 0.14 -0.4 352,221 + GFF361 0.14 +1.3 352,221 + GFF361 0.14 -0.7 352,221 + GFF361 0.14 +1.6 352,229 - GFF361 0.15 -0.8 352,229 - GFF361 0.15 +1.4 352,229 - GFF361 0.15 -0.2 352,231 + GFF361 0.16 +1.5 352,246 + GFF361 0.18 -0.6 352,252 + GFF361 0.18 -0.2 352,260 + GFF361 0.20 +0.1 352,260 + GFF361 0.20 -0.1 352,268 - GFF361 0.21 +0.6 352,268 - GFF361 0.21 -0.0 352,273 - GFF361 0.21 +1.1 352,273 - GFF361 0.21 -1.8 352,288 - GFF361 0.23 +0.5 352,291 + GFF361 0.24 +0.8 352,291 + GFF361 0.24 +0.7 352,291 + GFF361 0.24 -0.2 352,296 + GFF361 0.24 -0.7 352,298 - GFF361 0.25 +0.3 352,299 - GFF361 0.25 -1.1 352,321 - GFF361 0.28 +0.8 352,338 + GFF361 0.30 +0.2 352,338 + GFF361 0.30 -1.7 352,338 + GFF361 0.30 +0.7 352,338 + GFF361 0.30 -0.5 352,338 + GFF361 0.30 -1.9 352,338 + GFF361 0.30 -1.2 352,338 + GFF361 0.30 +0.0 352,338 + GFF361 0.30 +0.1 352,340 - GFF361 0.30 +0.6 352,341 - GFF361 0.31 +0.8 352,343 + GFF361 0.31 -2.0 352,346 - GFF361 0.31 +0.0 352,346 - GFF361 0.31 +0.7 352,349 + GFF361 0.32 -1.7 352,354 + GFF361 0.32 -1.9 352,354 + GFF361 0.32 +0.5 352,355 + GFF361 0.33 +0.3 352,355 + GFF361 0.33 -0.4 352,355 + GFF361 0.33 +0.0 352,355 + GFF361 0.33 -1.7 352,355 + GFF361 0.33 -2.7 352,359 + GFF361 0.33 -1.8 352,362 - GFF361 0.33 -0.2 352,363 - GFF361 0.34 -0.2 352,363 - GFF361 0.34 -0.5 352,363 - GFF361 0.34 -1.8 352,363 - GFF361 0.34 +0.0 352,365 + GFF361 0.34 -1.8 352,386 + GFF361 0.37 -0.1 352,386 + GFF361 0.37 -0.2 352,386 + GFF361 0.37 -0.3 352,391 + GFF361 0.37 +0.2 352,399 - GFF361 0.39 -2.0 352,399 - GFF361 0.39 -0.3 352,434 + GFF361 0.43 -0.4 352,462 + GFF361 0.47 +1.0 352,462 + GFF361 0.47 +0.1 352,463 - GFF361 0.47 +0.2 352,470 - GFF361 0.48 -2.2 352,476 - GFF361 0.49 +1.3 352,498 - GFF361 0.52 +0.3 352,501 + GFF361 0.52 -2.6 352,519 + GFF361 0.55 +0.6 352,522 - GFF361 0.55 -0.1 352,529 + GFF361 0.56 -0.2 352,529 + GFF361 0.56 +0.4 352,537 - GFF361 0.57 +0.0 352,537 - GFF361 0.57 -0.7 352,537 - GFF361 0.57 -1.4 352,537 - GFF361 0.57 -0.4 352,556 + GFF361 0.60 +0.5 352,562 - GFF361 0.61 -1.5 352,584 + GFF361 0.64 -0.3 352,592 - GFF361 0.65 -0.5 352,592 - GFF361 0.65 +0.3 352,599 + GFF361 0.66 +1.0 352,601 + GFF361 0.66 -1.3 352,601 + GFF361 0.66 -1.1 352,601 + GFF361 0.66 -0.7 352,601 + GFF361 0.66 -1.3 352,601 + GFF361 0.66 +0.6 352,601 + GFF361 0.66 -0.5 352,601 + GFF361 0.66 +1.0 352,601 + GFF361 0.66 -0.5 352,609 - GFF361 0.67 +0.8 352,609 - GFF361 0.67 -0.7 352,609 - GFF361 0.67 -1.5 352,609 - GFF361 0.67 -0.2 352,609 - GFF361 0.67 +2.2 352,609 - GFF361 0.67 +1.2 352,609 - GFF361 0.67 -1.0 352,609 - GFF361 0.67 +0.9 352,609 - GFF361 0.67 -2.2 352,614 - GFF361 0.68 -1.2 352,636 - GFF361 0.71 -0.8 352,646 + GFF361 0.72 -1.4 352,662 - GFF361 0.74 -0.1
Or see this region's nucleotide sequence