Experiment: EMM_noCarbon with Sucrose and Putrescine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2348 and GFF2349 are separated by 9 nucleotides GFF2349 and GFF2350 are separated by 22 nucleotides GFF2350 and GFF2351 overlap by 1 nucleotides GFF2351 and GFF2352 are separated by 31 nucleotides GFF2352 and GFF2353 are separated by 3 nucleotides
GFF2348 - Thymidine kinase (EC 2.7.1.21), at 2,372,641 to 2,373,219
GFF2348
GFF2349 - hypothetical protein, at 2,373,229 to 2,374,125
GFF2349
GFF2350 - PilT protein-like, at 2,374,148 to 2,374,537
GFF2350
GFF2351 - hypothetical protein, at 2,374,537 to 2,374,806
GFF2351
GFF2352 - Ribosome-binding factor A, at 2,374,838 to 2,375,239
GFF2352
GFF2353 - Phage-related transcriptional regulator, at 2,375,243 to 2,375,617
GFF2353
Position (kb)
2374
2375 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2373.167 kb on - strand at 2373.168 kb on - strand at 2373.168 kb on - strand at 2373.185 kb on + strand at 2373.193 kb on + strand at 2373.195 kb on - strand at 2373.211 kb on - strand at 2373.211 kb on - strand at 2373.211 kb on - strand at 2373.227 kb on + strand at 2373.227 kb on + strand at 2373.227 kb on + strand at 2373.235 kb on - strand at 2373.235 kb on - strand at 2373.235 kb on - strand at 2373.235 kb on - strand at 2373.260 kb on + strand at 2373.312 kb on - strand at 2373.312 kb on - strand at 2373.345 kb on - strand, within GFF2349 at 2373.358 kb on - strand, within GFF2349 at 2373.376 kb on - strand, within GFF2349 at 2373.391 kb on + strand, within GFF2349 at 2373.396 kb on + strand, within GFF2349 at 2373.399 kb on - strand, within GFF2349 at 2373.431 kb on + strand, within GFF2349 at 2373.461 kb on - strand, within GFF2349 at 2373.461 kb on - strand, within GFF2349 at 2373.473 kb on + strand, within GFF2349 at 2373.480 kb on - strand, within GFF2349 at 2373.481 kb on - strand, within GFF2349 at 2373.481 kb on - strand, within GFF2349 at 2373.538 kb on - strand, within GFF2349 at 2373.579 kb on + strand, within GFF2349 at 2373.605 kb on - strand, within GFF2349 at 2373.605 kb on - strand, within GFF2349 at 2373.611 kb on + strand, within GFF2349 at 2373.619 kb on - strand, within GFF2349 at 2373.644 kb on + strand, within GFF2349 at 2373.664 kb on + strand, within GFF2349 at 2373.671 kb on + strand, within GFF2349 at 2373.679 kb on + strand, within GFF2349 at 2373.703 kb on + strand, within GFF2349 at 2373.727 kb on + strand, within GFF2349 at 2373.727 kb on + strand, within GFF2349 at 2373.728 kb on + strand, within GFF2349 at 2373.735 kb on - strand, within GFF2349 at 2373.738 kb on + strand, within GFF2349 at 2373.795 kb on + strand, within GFF2349 at 2373.800 kb on + strand, within GFF2349 at 2373.803 kb on - strand, within GFF2349 at 2373.805 kb on + strand, within GFF2349 at 2373.813 kb on - strand, within GFF2349 at 2373.813 kb on - strand, within GFF2349 at 2373.829 kb on + strand, within GFF2349 at 2373.829 kb on + strand, within GFF2349 at 2373.829 kb on + strand, within GFF2349 at 2373.829 kb on + strand, within GFF2349 at 2373.829 kb on + strand, within GFF2349 at 2373.829 kb on + strand, within GFF2349 at 2373.830 kb on - strand, within GFF2349 at 2373.830 kb on - strand, within GFF2349 at 2373.837 kb on - strand, within GFF2349 at 2373.837 kb on - strand, within GFF2349 at 2373.846 kb on - strand, within GFF2349 at 2373.860 kb on + strand, within GFF2349 at 2373.860 kb on + strand, within GFF2349 at 2373.860 kb on + strand, within GFF2349 at 2373.860 kb on + strand, within GFF2349 at 2373.868 kb on - strand, within GFF2349 at 2373.868 kb on - strand, within GFF2349 at 2373.868 kb on - strand, within GFF2349 at 2373.868 kb on - strand, within GFF2349 at 2373.868 kb on - strand, within GFF2349 at 2373.870 kb on + strand, within GFF2349 at 2373.878 kb on - strand, within GFF2349 at 2373.898 kb on + strand, within GFF2349 at 2373.898 kb on + strand, within GFF2349 at 2373.907 kb on - strand, within GFF2349 at 2373.916 kb on + strand, within GFF2349 at 2373.916 kb on + strand, within GFF2349 at 2373.917 kb on + strand, within GFF2349 at 2373.921 kb on + strand, within GFF2349 at 2373.921 kb on + strand, within GFF2349 at 2373.923 kb on - strand, within GFF2349 at 2373.929 kb on + strand, within GFF2349 at 2373.944 kb on + strand, within GFF2349 at 2373.944 kb on + strand, within GFF2349 at 2373.954 kb on - strand, within GFF2349 at 2373.954 kb on - strand, within GFF2349 at 2373.959 kb on + strand, within GFF2349 at 2374.001 kb on - strand, within GFF2349 at 2374.016 kb on - strand, within GFF2349 at 2374.042 kb on - strand at 2374.067 kb on + strand at 2374.077 kb on + strand at 2374.077 kb on + strand at 2374.085 kb on + strand at 2374.085 kb on + strand at 2374.090 kb on + strand at 2374.090 kb on + strand at 2374.090 kb on + strand at 2374.090 kb on + strand at 2374.090 kb on + strand at 2374.090 kb on + strand at 2374.093 kb on - strand at 2374.093 kb on - strand at 2374.095 kb on + strand at 2374.098 kb on - strand at 2374.098 kb on - strand at 2374.098 kb on - strand at 2374.098 kb on - strand at 2374.098 kb on - strand at 2374.098 kb on - strand at 2374.098 kb on - strand at 2374.098 kb on - strand at 2374.098 kb on - strand at 2374.098 kb on - strand at 2374.098 kb on - strand at 2374.098 kb on - strand at 2374.103 kb on - strand at 2374.118 kb on - strand at 2374.165 kb on - strand at 2374.207 kb on + strand, within GFF2350 at 2374.215 kb on - strand, within GFF2350 at 2374.216 kb on + strand, within GFF2350 at 2374.224 kb on - strand, within GFF2350 at 2374.239 kb on + strand, within GFF2350 at 2374.239 kb on + strand, within GFF2350 at 2374.239 kb on + strand, within GFF2350 at 2374.239 kb on + strand, within GFF2350 at 2374.247 kb on - strand, within GFF2350 at 2374.247 kb on - strand, within GFF2350 at 2374.247 kb on - strand, within GFF2350 at 2374.247 kb on - strand, within GFF2350 at 2374.247 kb on - strand, within GFF2350 at 2374.247 kb on - strand, within GFF2350 at 2374.247 kb on - strand, within GFF2350 at 2374.257 kb on - strand, within GFF2350 at 2374.259 kb on - strand, within GFF2350 at 2374.300 kb on + strand, within GFF2350 at 2374.334 kb on - strand, within GFF2350 at 2374.336 kb on + strand, within GFF2350 at 2374.359 kb on + strand, within GFF2350 at 2374.359 kb on + strand, within GFF2350 at 2374.359 kb on + strand, within GFF2350 at 2374.359 kb on + strand, within GFF2350 at 2374.359 kb on + strand, within GFF2350 at 2374.367 kb on - strand, within GFF2350 at 2374.367 kb on - strand, within GFF2350 at 2374.367 kb on - strand, within GFF2350 at 2374.367 kb on - strand, within GFF2350 at 2374.367 kb on - strand, within GFF2350 at 2374.367 kb on - strand, within GFF2350 at 2374.367 kb on - strand, within GFF2350 at 2374.367 kb on - strand, within GFF2350 at 2374.386 kb on - strand, within GFF2350 at 2374.395 kb on + strand, within GFF2350 at 2374.395 kb on + strand, within GFF2350 at 2374.402 kb on - strand, within GFF2350 at 2374.404 kb on - strand, within GFF2350 at 2374.417 kb on - strand, within GFF2350 at 2374.433 kb on - strand, within GFF2350 at 2374.442 kb on + strand, within GFF2350 at 2374.459 kb on - strand, within GFF2350 at 2374.494 kb on + strand, within GFF2350 at 2374.494 kb on + strand, within GFF2350 at 2374.502 kb on - strand at 2374.502 kb on - strand at 2374.505 kb on + strand at 2374.535 kb on + strand at 2374.603 kb on + strand, within GFF2351 at 2374.616 kb on - strand, within GFF2351 at 2374.617 kb on + strand, within GFF2351 at 2374.623 kb on + strand, within GFF2351 at 2374.627 kb on + strand, within GFF2351 at 2374.627 kb on + strand, within GFF2351 at 2374.634 kb on + strand, within GFF2351 at 2374.635 kb on - strand, within GFF2351 at 2374.644 kb on + strand, within GFF2351 at 2374.644 kb on + strand, within GFF2351 at 2374.652 kb on - strand, within GFF2351 at 2374.652 kb on - strand, within GFF2351 at 2374.659 kb on - strand, within GFF2351 at 2374.664 kb on + strand, within GFF2351 at 2374.672 kb on - strand, within GFF2351 at 2374.690 kb on + strand, within GFF2351 at 2374.711 kb on + strand, within GFF2351 at 2374.728 kb on + strand, within GFF2351 at 2374.731 kb on - strand, within GFF2351 at 2374.735 kb on + strand, within GFF2351 at 2374.736 kb on - strand, within GFF2351 at 2374.736 kb on - strand, within GFF2351 at 2374.770 kb on - strand, within GFF2351 at 2374.776 kb on - strand, within GFF2351 at 2374.792 kb on + strand at 2374.793 kb on + strand at 2374.796 kb on - strand at 2374.796 kb on - strand at 2374.801 kb on - strand at 2374.801 kb on - strand at 2374.848 kb on + strand at 2374.848 kb on + strand at 2374.848 kb on + strand at 2374.854 kb on + strand at 2374.866 kb on - strand at 2374.866 kb on - strand at 2374.866 kb on - strand at 2374.876 kb on + strand at 2374.884 kb on - strand, within GFF2352 at 2375.397 kb on - strand, within GFF2353
Per-strain Table
Position Strand Gene LocusTag Fraction EMM_noCarbon with Sucrose and Putrescine remove 2,373,167 - -0.4 2,373,168 - -1.1 2,373,168 - -0.3 2,373,185 + -0.3 2,373,193 + -0.2 2,373,195 - -0.4 2,373,211 - +0.6 2,373,211 - +0.8 2,373,211 - -0.2 2,373,227 + -0.8 2,373,227 + -2.1 2,373,227 + -1.2 2,373,235 - -0.0 2,373,235 - -0.3 2,373,235 - +0.3 2,373,235 - +0.2 2,373,260 + -0.0 2,373,312 - -1.1 2,373,312 - +1.0 2,373,345 - GFF2349 0.13 +0.4 2,373,358 - GFF2349 0.14 -0.2 2,373,376 - GFF2349 0.16 -0.5 2,373,391 + GFF2349 0.18 -1.3 2,373,396 + GFF2349 0.19 -0.4 2,373,399 - GFF2349 0.19 -0.1 2,373,431 + GFF2349 0.23 -0.0 2,373,461 - GFF2349 0.26 +0.1 2,373,461 - GFF2349 0.26 -1.5 2,373,473 + GFF2349 0.27 +1.9 2,373,480 - GFF2349 0.28 +0.5 2,373,481 - GFF2349 0.28 -0.0 2,373,481 - GFF2349 0.28 +1.5 2,373,538 - GFF2349 0.34 -0.4 2,373,579 + GFF2349 0.39 -1.8 2,373,605 - GFF2349 0.42 -0.7 2,373,605 - GFF2349 0.42 +0.6 2,373,611 + GFF2349 0.43 -0.2 2,373,619 - GFF2349 0.43 +0.4 2,373,644 + GFF2349 0.46 +0.1 2,373,664 + GFF2349 0.48 -0.3 2,373,671 + GFF2349 0.49 -1.2 2,373,679 + GFF2349 0.50 -0.6 2,373,703 + GFF2349 0.53 -1.2 2,373,727 + GFF2349 0.56 +0.4 2,373,727 + GFF2349 0.56 -0.0 2,373,728 + GFF2349 0.56 -0.9 2,373,735 - GFF2349 0.56 -0.9 2,373,738 + GFF2349 0.57 -2.1 2,373,795 + GFF2349 0.63 +0.6 2,373,800 + GFF2349 0.64 +1.1 2,373,803 - GFF2349 0.64 -0.8 2,373,805 + GFF2349 0.64 +0.0 2,373,813 - GFF2349 0.65 -1.2 2,373,813 - GFF2349 0.65 -0.2 2,373,829 + GFF2349 0.67 -1.5 2,373,829 + GFF2349 0.67 -0.9 2,373,829 + GFF2349 0.67 -0.7 2,373,829 + GFF2349 0.67 +1.6 2,373,829 + GFF2349 0.67 -0.3 2,373,829 + GFF2349 0.67 +0.7 2,373,830 - GFF2349 0.67 -0.2 2,373,830 - GFF2349 0.67 +0.5 2,373,837 - GFF2349 0.68 +0.9 2,373,837 - GFF2349 0.68 -0.1 2,373,846 - GFF2349 0.69 +0.4 2,373,860 + GFF2349 0.70 -0.1 2,373,860 + GFF2349 0.70 -0.9 2,373,860 + GFF2349 0.70 +0.2 2,373,860 + GFF2349 0.70 +1.1 2,373,868 - GFF2349 0.71 -2.6 2,373,868 - GFF2349 0.71 +0.3 2,373,868 - GFF2349 0.71 -0.7 2,373,868 - GFF2349 0.71 -0.3 2,373,868 - GFF2349 0.71 +1.6 2,373,870 + GFF2349 0.71 -1.5 2,373,878 - GFF2349 0.72 +0.2 2,373,898 + GFF2349 0.75 -1.9 2,373,898 + GFF2349 0.75 -0.9 2,373,907 - GFF2349 0.76 -0.2 2,373,916 + GFF2349 0.77 +0.6 2,373,916 + GFF2349 0.77 +1.9 2,373,917 + GFF2349 0.77 -0.6 2,373,921 + GFF2349 0.77 -0.6 2,373,921 + GFF2349 0.77 +0.1 2,373,923 - GFF2349 0.77 -1.6 2,373,929 + GFF2349 0.78 -2.5 2,373,944 + GFF2349 0.80 +0.6 2,373,944 + GFF2349 0.80 +0.5 2,373,954 - GFF2349 0.81 +0.3 2,373,954 - GFF2349 0.81 -0.6 2,373,959 + GFF2349 0.81 -2.4 2,374,001 - GFF2349 0.86 -0.4 2,374,016 - GFF2349 0.88 +1.0 2,374,042 - -2.6 2,374,067 + +1.0 2,374,077 + +0.4 2,374,077 + -1.2 2,374,085 + -1.5 2,374,085 + -0.7 2,374,090 + -1.2 2,374,090 + +0.5 2,374,090 + -1.2 2,374,090 + -0.2 2,374,090 + -3.0 2,374,090 + -2.2 2,374,093 - -0.5 2,374,093 - -0.1 2,374,095 + +0.6 2,374,098 - -0.2 2,374,098 - -0.7 2,374,098 - +0.4 2,374,098 - -0.6 2,374,098 - -0.4 2,374,098 - -0.5 2,374,098 - -0.2 2,374,098 - +0.7 2,374,098 - +1.1 2,374,098 - -0.8 2,374,098 - -1.9 2,374,098 - -2.1 2,374,103 - -0.1 2,374,118 - -0.7 2,374,165 - -1.9 2,374,207 + GFF2350 0.15 -0.3 2,374,215 - GFF2350 0.17 +0.2 2,374,216 + GFF2350 0.17 -1.8 2,374,224 - GFF2350 0.19 -1.2 2,374,239 + GFF2350 0.23 -0.1 2,374,239 + GFF2350 0.23 -2.1 2,374,239 + GFF2350 0.23 -1.1 2,374,239 + GFF2350 0.23 -0.4 2,374,247 - GFF2350 0.25 +0.4 2,374,247 - GFF2350 0.25 -1.5 2,374,247 - GFF2350 0.25 +0.1 2,374,247 - GFF2350 0.25 -1.5 2,374,247 - GFF2350 0.25 +0.4 2,374,247 - GFF2350 0.25 -0.0 2,374,247 - GFF2350 0.25 -1.8 2,374,257 - GFF2350 0.28 -0.8 2,374,259 - GFF2350 0.28 -0.2 2,374,300 + GFF2350 0.39 -0.2 2,374,334 - GFF2350 0.48 +0.8 2,374,336 + GFF2350 0.48 -1.2 2,374,359 + GFF2350 0.54 +0.6 2,374,359 + GFF2350 0.54 -1.7 2,374,359 + GFF2350 0.54 +0.3 2,374,359 + GFF2350 0.54 +0.9 2,374,359 + GFF2350 0.54 -1.4 2,374,367 - GFF2350 0.56 +1.2 2,374,367 - GFF2350 0.56 -0.5 2,374,367 - GFF2350 0.56 +0.6 2,374,367 - GFF2350 0.56 -0.0 2,374,367 - GFF2350 0.56 +0.0 2,374,367 - GFF2350 0.56 -0.2 2,374,367 - GFF2350 0.56 +0.7 2,374,367 - GFF2350 0.56 +0.1 2,374,386 - GFF2350 0.61 -0.6 2,374,395 + GFF2350 0.63 -0.5 2,374,395 + GFF2350 0.63 -1.9 2,374,402 - GFF2350 0.65 -0.9 2,374,404 - GFF2350 0.66 -2.0 2,374,417 - GFF2350 0.69 +0.9 2,374,433 - GFF2350 0.73 +0.4 2,374,442 + GFF2350 0.75 +2.2 2,374,459 - GFF2350 0.80 -1.6 2,374,494 + GFF2350 0.89 -0.9 2,374,494 + GFF2350 0.89 -0.6 2,374,502 - +0.3 2,374,502 - -0.2 2,374,505 + +0.1 2,374,535 + +0.1 2,374,603 + GFF2351 0.24 -0.1 2,374,616 - GFF2351 0.29 +1.4 2,374,617 + GFF2351 0.30 -1.2 2,374,623 + GFF2351 0.32 +1.3 2,374,627 + GFF2351 0.33 +0.3 2,374,627 + GFF2351 0.33 -1.2 2,374,634 + GFF2351 0.36 +0.7 2,374,635 - GFF2351 0.36 -2.0 2,374,644 + GFF2351 0.40 -0.8 2,374,644 + GFF2351 0.40 -0.6 2,374,652 - GFF2351 0.43 -1.6 2,374,652 - GFF2351 0.43 -1.4 2,374,659 - GFF2351 0.45 +0.3 2,374,664 + GFF2351 0.47 -1.1 2,374,672 - GFF2351 0.50 -0.8 2,374,690 + GFF2351 0.57 -1.1 2,374,711 + GFF2351 0.64 +0.1 2,374,728 + GFF2351 0.71 +0.2 2,374,731 - GFF2351 0.72 +1.2 2,374,735 + GFF2351 0.73 +0.8 2,374,736 - GFF2351 0.74 -0.6 2,374,736 - GFF2351 0.74 +1.3 2,374,770 - GFF2351 0.86 +0.4 2,374,776 - GFF2351 0.89 +0.0 2,374,792 + +1.4 2,374,793 + -0.0 2,374,796 - +0.1 2,374,796 - -0.8 2,374,801 - -0.8 2,374,801 - -1.4 2,374,848 + -1.1 2,374,848 + -0.7 2,374,848 + +0.8 2,374,854 + +0.6 2,374,866 - -0.9 2,374,866 - +0.0 2,374,866 - -0.7 2,374,876 + +1.2 2,374,884 - GFF2352 0.11 -1.6 2,375,397 - GFF2353 0.41 +0.5
Or see this region's nucleotide sequence