Experiment: EMM_noCarbon with Sucrose and Putrescine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF80 and GFF81 are separated by 2 nucleotides GFF81 and GFF82 overlap by 4 nucleotides GFF82 and GFF83 overlap by 4 nucleotides GFF83 and GFF84 overlap by 4 nucleotides GFF84 and GFF85 overlap by 4 nucleotides GFF85 and GFF86 overlap by 4 nucleotides
GFF80 - Cytochrome c-type biogenesis protein CcmC, putative heme lyase for CcmE, at 70,214 to 70,936
GFF80
GFF81 - hypothetical protein, at 70,939 to 71,070
GFF81
GFF82 - Cytochrome c-type biogenesis protein CcmE, heme chaperone, at 71,067 to 71,513
GFF82
GFF83 - Cytochrome c heme lyase subunit CcmF, at 71,510 to 73,438
GFF83
GFF84 - Cytochrome c-type biogenesis protein CcmG/DsbE, thiol:disulfide oxidoreductase, at 73,435 to 73,962
GFF84
GFF85 - Cytochrome c heme lyase subunit CcmL, at 73,959 to 74,372
GFF85
GFF86 - Cytochrome c heme lyase subunit CcmH, at 74,369 to 75,010
GFF86
Position (kb)
71
72
73
74 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 70.931 kb on + strand at 70.931 kb on + strand at 70.931 kb on + strand at 70.931 kb on + strand at 70.931 kb on + strand at 70.936 kb on - strand at 70.939 kb on - strand at 70.939 kb on - strand at 70.939 kb on - strand at 70.941 kb on - strand at 71.045 kb on - strand, within GFF81 at 71.051 kb on - strand, within GFF81 at 71.066 kb on + strand at 71.072 kb on + strand at 71.495 kb on + strand at 71.495 kb on + strand at 71.503 kb on - strand at 71.503 kb on - strand at 71.503 kb on - strand at 73.378 kb on + strand at 73.378 kb on + strand at 73.378 kb on + strand at 73.378 kb on + strand at 73.378 kb on + strand at 73.378 kb on + strand at 73.383 kb on + strand at 73.383 kb on + strand at 73.386 kb on - strand at 73.386 kb on - strand at 73.393 kb on + strand at 73.425 kb on + strand at 73.425 kb on + strand at 73.425 kb on + strand at 73.425 kb on + strand at 73.425 kb on + strand at 73.425 kb on + strand at 73.433 kb on - strand at 73.444 kb on + strand at 73.444 kb on + strand at 73.944 kb on + strand at 73.944 kb on + strand at 73.950 kb on - strand at 73.952 kb on - strand at 73.952 kb on - strand at 73.952 kb on - strand at 73.961 kb on + strand at 73.961 kb on + strand at 73.961 kb on + strand at 73.969 kb on - strand at 73.973 kb on + strand at 74.356 kb on - strand at 74.356 kb on - strand at 74.361 kb on - strand at 74.361 kb on - strand at 74.414 kb on + strand at 74.414 kb on + strand at 74.419 kb on + strand at 74.420 kb on - strand at 74.424 kb on + strand at 74.424 kb on + strand at 74.424 kb on + strand at 74.424 kb on + strand at 74.424 kb on + strand at 74.432 kb on - strand at 74.437 kb on + strand, within GFF86
Per-strain Table
Position Strand Gene LocusTag Fraction EMM_noCarbon with Sucrose and Putrescine remove 70,931 + +1.0 70,931 + -0.4 70,931 + -0.4 70,931 + +0.9 70,931 + +1.5 70,936 - -0.2 70,939 - -0.6 70,939 - -1.4 70,939 - -1.9 70,941 - -1.1 71,045 - GFF81 0.80 -2.3 71,051 - GFF81 0.85 -2.0 71,066 + -0.7 71,072 + -1.0 71,495 + -0.7 71,495 + -1.9 71,503 - +0.2 71,503 - -0.0 71,503 - -1.2 73,378 + -1.0 73,378 + +0.3 73,378 + -1.6 73,378 + +0.6 73,378 + -1.6 73,378 + -0.6 73,383 + -1.8 73,383 + -1.4 73,386 - +1.0 73,386 - -2.0 73,393 + -1.0 73,425 + +0.4 73,425 + -2.4 73,425 + -0.3 73,425 + -1.6 73,425 + -0.0 73,425 + -1.1 73,433 - -3.3 73,444 + -2.1 73,444 + -1.2 73,944 + -0.4 73,944 + -1.6 73,950 - -1.9 73,952 - -1.2 73,952 - -0.1 73,952 - -2.2 73,961 + -2.0 73,961 + -1.9 73,961 + -0.2 73,969 - -1.8 73,973 + -1.5 74,356 - -2.6 74,356 - -1.0 74,361 - -0.0 74,361 - -1.9 74,414 + -1.0 74,414 + +1.4 74,419 + +0.1 74,420 - -0.1 74,424 + -1.0 74,424 + -1.9 74,424 + -2.1 74,424 + -1.2 74,424 + -1.4 74,432 - +0.3 74,437 + GFF86 0.11 -0.1
Or see this region's nucleotide sequence