Strain Fitness in Sphingobium sp. HT1-2 around GFF82

Experiment: EMM_noCarbon with Sucrose and Putrescine

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF80 and GFF81 are separated by 2 nucleotidesGFF81 and GFF82 overlap by 4 nucleotidesGFF82 and GFF83 overlap by 4 nucleotides GFF80 - Cytochrome c-type biogenesis protein CcmC, putative heme lyase for CcmE, at 70,214 to 70,936 GFF80 GFF81 - hypothetical protein, at 70,939 to 71,070 GFF81 GFF82 - Cytochrome c-type biogenesis protein CcmE, heme chaperone, at 71,067 to 71,513 GFF82 GFF83 - Cytochrome c heme lyase subunit CcmF, at 71,510 to 73,438 GFF83 Position (kb) 71 72Strain fitness (log2 ratio) -3 -2 -1 0 1at 70.070 kb on + strandat 70.070 kb on + strandat 70.070 kb on + strandat 70.070 kb on + strandat 70.070 kb on + strandat 70.071 kb on + strandat 70.076 kb on - strandat 70.078 kb on - strandat 70.078 kb on - strandat 70.078 kb on - strandat 70.120 kb on + strandat 70.129 kb on + strandat 70.129 kb on + strandat 70.129 kb on + strandat 70.205 kb on + strandat 70.205 kb on + strandat 70.931 kb on + strandat 70.931 kb on + strandat 70.931 kb on + strandat 70.931 kb on + strandat 70.931 kb on + strandat 70.936 kb on - strandat 70.939 kb on - strandat 70.939 kb on - strandat 70.939 kb on - strandat 70.941 kb on - strandat 71.045 kb on - strand, within GFF81at 71.051 kb on - strand, within GFF81at 71.066 kb on + strandat 71.072 kb on + strandat 71.495 kb on + strandat 71.495 kb on + strandat 71.503 kb on - strandat 71.503 kb on - strandat 71.503 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction EMM_noCarbon with Sucrose and Putrescine
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70,070 + -1.8
70,070 + -1.4
70,070 + -1.4
70,070 + -2.5
70,070 + -2.7
70,071 + -2.8
70,076 - -0.9
70,078 - +0.5
70,078 - -1.6
70,078 - +0.2
70,120 + -2.5
70,129 + -0.2
70,129 + +0.2
70,129 + -0.4
70,205 + +0.8
70,205 + -1.0
70,931 + +1.0
70,931 + -0.4
70,931 + -0.4
70,931 + +0.9
70,931 + +1.5
70,936 - -0.2
70,939 - -0.6
70,939 - -1.4
70,939 - -1.9
70,941 - -1.1
71,045 - GFF81 0.80 -2.3
71,051 - GFF81 0.85 -2.0
71,066 + -0.7
71,072 + -1.0
71,495 + -0.7
71,495 + -1.9
71,503 - +0.2
71,503 - -0.0
71,503 - -1.2

Or see this region's nucleotide sequence