Experiment: EMM_noCarbon with Sucrose and Putrescine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF5 and GFF6 are separated by 455 nucleotides GFF6 and GFF7 are separated by 157 nucleotides GFF7 and GFF8 are separated by 281 nucleotides GFF8 and GFF9 are separated by 88 nucleotides GFF9 and GFF10 are separated by 93 nucleotides
GFF5 - DNA-binding protein, at 3,351 to 3,650
GFF5
GFF6 - hypothetical protein, at 4,106 to 4,231
GFF6
GFF7 - hypothetical protein, at 4,389 to 4,778
GFF7
GFF8 - hypothetical protein, at 5,060 to 5,461
GFF8
GFF9 - hypothetical protein, at 5,550 to 5,663
GFF9
GFF10 - hypothetical protein, at 5,757 to 6,065
GFF10
Position (kb) 4
5 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 3.435 kb on - strand, within GFF5 at 3.493 kb on + strand, within GFF5 at 3.508 kb on + strand, within GFF5 at 3.508 kb on + strand, within GFF5 at 3.528 kb on + strand, within GFF5 at 3.528 kb on + strand, within GFF5 at 3.540 kb on + strand, within GFF5 at 3.540 kb on + strand, within GFF5 at 3.540 kb on + strand, within GFF5 at 3.651 kb on + strand at 3.657 kb on - strand at 3.740 kb on - strand at 3.745 kb on - strand at 3.774 kb on - strand at 3.776 kb on + strand at 3.776 kb on + strand at 3.805 kb on + strand at 3.809 kb on + strand at 3.811 kb on + strand at 3.824 kb on + strand at 3.824 kb on + strand at 3.824 kb on + strand at 3.824 kb on + strand at 3.824 kb on + strand at 3.825 kb on - strand at 3.829 kb on + strand at 3.829 kb on + strand at 3.832 kb on - strand at 3.837 kb on - strand at 3.838 kb on - strand at 3.838 kb on - strand at 3.838 kb on - strand at 3.844 kb on + strand at 3.844 kb on + strand at 3.852 kb on - strand at 3.855 kb on - strand at 3.885 kb on - strand at 3.893 kb on + strand at 3.898 kb on + strand at 3.898 kb on + strand at 3.898 kb on + strand at 3.903 kb on + strand at 3.906 kb on - strand at 3.951 kb on - strand at 3.955 kb on + strand at 3.958 kb on - strand at 3.963 kb on - strand at 3.970 kb on + strand at 3.970 kb on + strand at 3.970 kb on - strand at 3.978 kb on + strand at 3.978 kb on - strand at 3.992 kb on - strand at 3.993 kb on - strand at 4.004 kb on + strand at 4.020 kb on - strand at 4.036 kb on + strand at 4.043 kb on - strand at 4.078 kb on + strand at 4.078 kb on + strand at 4.083 kb on + strand at 4.086 kb on - strand at 4.086 kb on - strand at 4.086 kb on - strand at 4.086 kb on - strand at 4.088 kb on + strand at 4.096 kb on - strand at 4.109 kb on + strand at 4.117 kb on - strand at 4.137 kb on + strand, within GFF6 at 4.153 kb on - strand, within GFF6 at 4.164 kb on - strand, within GFF6 at 4.178 kb on + strand, within GFF6 at 4.209 kb on + strand, within GFF6 at 4.212 kb on - strand, within GFF6 at 4.212 kb on - strand, within GFF6 at 4.212 kb on - strand, within GFF6 at 4.212 kb on - strand, within GFF6 at 4.217 kb on - strand, within GFF6 at 4.217 kb on - strand, within GFF6 at 4.219 kb on + strand at 4.247 kb on - strand at 4.292 kb on + strand at 4.294 kb on - strand at 4.302 kb on - strand at 4.309 kb on + strand at 4.314 kb on + strand at 4.314 kb on + strand at 4.316 kb on + strand at 4.317 kb on - strand at 4.317 kb on - strand at 4.322 kb on - strand at 4.322 kb on - strand at 4.322 kb on - strand at 4.322 kb on - strand at 4.381 kb on + strand at 4.414 kb on + strand at 4.415 kb on + strand at 4.422 kb on - strand at 4.442 kb on + strand, within GFF7 at 4.457 kb on + strand, within GFF7 at 4.518 kb on + strand, within GFF7 at 4.521 kb on + strand, within GFF7 at 4.526 kb on - strand, within GFF7 at 4.539 kb on + strand, within GFF7 at 4.549 kb on + strand, within GFF7 at 4.554 kb on + strand, within GFF7 at 4.554 kb on + strand, within GFF7 at 4.562 kb on - strand, within GFF7 at 4.562 kb on - strand, within GFF7 at 4.588 kb on + strand, within GFF7 at 4.588 kb on + strand, within GFF7 at 4.588 kb on + strand, within GFF7 at 4.588 kb on + strand, within GFF7 at 4.588 kb on + strand, within GFF7 at 4.588 kb on + strand, within GFF7 at 4.588 kb on + strand, within GFF7 at 4.588 kb on + strand, within GFF7 at 4.588 kb on + strand, within GFF7 at 4.588 kb on + strand, within GFF7 at 4.588 kb on + strand, within GFF7 at 4.588 kb on + strand, within GFF7 at 4.588 kb on + strand, within GFF7 at 4.596 kb on - strand, within GFF7 at 4.596 kb on - strand, within GFF7 at 4.596 kb on - strand, within GFF7 at 4.596 kb on - strand, within GFF7 at 4.596 kb on - strand, within GFF7 at 4.596 kb on - strand, within GFF7 at 4.596 kb on - strand, within GFF7 at 4.605 kb on - strand, within GFF7 at 4.630 kb on + strand, within GFF7 at 4.640 kb on + strand, within GFF7 at 4.647 kb on + strand, within GFF7 at 4.647 kb on + strand, within GFF7 at 4.647 kb on + strand, within GFF7 at 4.654 kb on + strand, within GFF7 at 4.654 kb on + strand, within GFF7 at 4.662 kb on - strand, within GFF7 at 4.668 kb on + strand, within GFF7 at 4.668 kb on + strand, within GFF7 at 4.676 kb on - strand, within GFF7 at 4.676 kb on - strand, within GFF7 at 4.676 kb on - strand, within GFF7 at 4.678 kb on + strand, within GFF7 at 4.678 kb on + strand, within GFF7 at 4.678 kb on + strand, within GFF7 at 4.686 kb on - strand, within GFF7 at 4.686 kb on - strand, within GFF7 at 4.686 kb on - strand, within GFF7 at 4.686 kb on - strand, within GFF7 at 4.686 kb on - strand, within GFF7 at 4.686 kb on - strand, within GFF7 at 4.686 kb on - strand, within GFF7 at 4.686 kb on - strand, within GFF7 at 4.687 kb on + strand, within GFF7 at 4.696 kb on + strand, within GFF7 at 4.705 kb on + strand, within GFF7 at 4.711 kb on - strand, within GFF7 at 4.717 kb on + strand, within GFF7 at 4.721 kb on + strand, within GFF7 at 4.737 kb on - strand, within GFF7 at 4.737 kb on - strand, within GFF7 at 4.737 kb on - strand, within GFF7 at 4.739 kb on + strand, within GFF7 at 4.739 kb on + strand, within GFF7 at 4.742 kb on - strand at 4.747 kb on + strand at 4.747 kb on - strand at 4.747 kb on - strand at 4.747 kb on - strand at 4.747 kb on - strand at 4.757 kb on - strand at 4.793 kb on + strand at 4.801 kb on - strand at 4.858 kb on - strand at 4.858 kb on - strand at 4.872 kb on + strand at 4.888 kb on - strand at 4.968 kb on + strand at 5.014 kb on - strand at 5.080 kb on + strand at 5.124 kb on - strand, within GFF8 at 5.124 kb on - strand, within GFF8 at 5.148 kb on - strand, within GFF8 at 5.151 kb on + strand, within GFF8 at 5.151 kb on + strand, within GFF8 at 5.151 kb on + strand, within GFF8 at 5.154 kb on - strand, within GFF8 at 5.175 kb on + strand, within GFF8 at 5.176 kb on + strand, within GFF8 at 5.176 kb on + strand, within GFF8 at 5.179 kb on - strand, within GFF8 at 5.192 kb on - strand, within GFF8 at 5.209 kb on + strand, within GFF8 at 5.210 kb on - strand, within GFF8 at 5.212 kb on + strand, within GFF8 at 5.212 kb on + strand, within GFF8 at 5.266 kb on - strand, within GFF8 at 5.279 kb on + strand, within GFF8 at 5.281 kb on - strand, within GFF8 at 5.284 kb on + strand, within GFF8 at 5.295 kb on + strand, within GFF8 at 5.295 kb on + strand, within GFF8 at 5.303 kb on - strand, within GFF8 at 5.308 kb on + strand, within GFF8 at 5.343 kb on + strand, within GFF8 at 5.348 kb on + strand, within GFF8 at 5.348 kb on + strand, within GFF8 at 5.356 kb on - strand, within GFF8 at 5.392 kb on + strand, within GFF8 at 5.392 kb on + strand, within GFF8 at 5.392 kb on + strand, within GFF8 at 5.400 kb on - strand, within GFF8 at 5.407 kb on + strand, within GFF8 at 5.431 kb on + strand at 5.435 kb on + strand at 5.443 kb on - strand at 5.447 kb on - strand at 5.464 kb on - strand at 5.464 kb on - strand at 5.480 kb on + strand at 5.508 kb on - strand at 5.631 kb on + strand, within GFF9 at 5.634 kb on - strand, within GFF9 at 5.634 kb on - strand, within GFF9 at 5.636 kb on + strand, within GFF9 at 5.636 kb on + strand, within GFF9 at 5.636 kb on + strand, within GFF9 at 5.644 kb on - strand, within GFF9 at 5.663 kb on - strand at 5.686 kb on - strand at 5.696 kb on + strand at 5.696 kb on + strand at 5.702 kb on - strand at 5.702 kb on - strand at 5.704 kb on - strand at 5.712 kb on - strand at 5.714 kb on - strand at 5.729 kb on - strand at 5.746 kb on + strand at 5.746 kb on + strand at 5.747 kb on + strand at 5.754 kb on - strand at 5.757 kb on - strand at 5.760 kb on + strand at 5.763 kb on - strand at 5.770 kb on - strand at 5.777 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction EMM_noCarbon with Sucrose and Putrescine remove 3,435 - GFF5 0.28 -2.2 3,493 + GFF5 0.47 -1.8 3,508 + GFF5 0.52 -1.4 3,508 + GFF5 0.52 -2.0 3,528 + GFF5 0.59 -1.5 3,528 + GFF5 0.59 -1.2 3,540 + GFF5 0.63 -1.4 3,540 + GFF5 0.63 -1.5 3,540 + GFF5 0.63 -1.9 3,651 + -1.9 3,657 - -1.9 3,740 - -2.0 3,745 - -1.0 3,774 - -0.8 3,776 + -1.6 3,776 + -2.2 3,805 + +1.3 3,809 + +1.1 3,811 + -0.5 3,824 + -0.7 3,824 + -0.2 3,824 + -0.6 3,824 + +0.1 3,824 + -0.1 3,825 - +1.1 3,829 + +0.3 3,829 + +1.2 3,832 - -0.0 3,837 - -1.7 3,838 - -1.1 3,838 - -0.5 3,838 - -0.2 3,844 + -0.4 3,844 + -0.2 3,852 - +1.2 3,855 - -2.1 3,885 - -1.8 3,893 + -0.0 3,898 + -0.5 3,898 + -1.4 3,898 + -0.2 3,903 + -0.4 3,906 - -1.4 3,951 - +0.6 3,955 + -0.5 3,958 - -0.6 3,963 - +0.6 3,970 + -2.5 3,970 + +2.0 3,970 - -1.2 3,978 + -2.0 3,978 - +1.0 3,992 - -0.0 3,993 - -1.1 4,004 + -0.6 4,020 - -0.9 4,036 + -0.3 4,043 - -1.5 4,078 + +0.5 4,078 + +0.5 4,083 + +1.6 4,086 - +1.1 4,086 - +0.9 4,086 - -2.7 4,086 - -0.6 4,088 + -1.7 4,096 - -0.1 4,109 + -1.1 4,117 - +0.2 4,137 + GFF6 0.25 -0.8 4,153 - GFF6 0.37 +1.2 4,164 - GFF6 0.46 +0.1 4,178 + GFF6 0.57 -1.0 4,209 + GFF6 0.82 +1.0 4,212 - GFF6 0.84 -0.8 4,212 - GFF6 0.84 +1.8 4,212 - GFF6 0.84 -0.9 4,212 - GFF6 0.84 +0.2 4,217 - GFF6 0.88 -0.7 4,217 - GFF6 0.88 +0.5 4,219 + +1.4 4,247 - -1.7 4,292 + -1.0 4,294 - -2.2 4,302 - -1.7 4,309 + -0.5 4,314 + +2.1 4,314 + -0.2 4,316 + -0.8 4,317 - +0.9 4,317 - -0.0 4,322 - -0.2 4,322 - -0.0 4,322 - -1.0 4,322 - -0.1 4,381 + -0.2 4,414 + -1.9 4,415 + -2.0 4,422 - -0.6 4,442 + GFF7 0.14 +0.5 4,457 + GFF7 0.17 -0.4 4,518 + GFF7 0.33 -1.2 4,521 + GFF7 0.34 -2.5 4,526 - GFF7 0.35 -0.5 4,539 + GFF7 0.38 -2.1 4,549 + GFF7 0.41 -1.2 4,554 + GFF7 0.42 -0.5 4,554 + GFF7 0.42 -1.9 4,562 - GFF7 0.44 +1.1 4,562 - GFF7 0.44 -1.6 4,588 + GFF7 0.51 -0.7 4,588 + GFF7 0.51 -1.0 4,588 + GFF7 0.51 +0.2 4,588 + GFF7 0.51 -1.0 4,588 + GFF7 0.51 -1.8 4,588 + GFF7 0.51 -0.5 4,588 + GFF7 0.51 -0.0 4,588 + GFF7 0.51 -1.5 4,588 + GFF7 0.51 +1.4 4,588 + GFF7 0.51 +0.5 4,588 + GFF7 0.51 -2.2 4,588 + GFF7 0.51 +0.6 4,588 + GFF7 0.51 -0.0 4,596 - GFF7 0.53 -0.4 4,596 - GFF7 0.53 -0.2 4,596 - GFF7 0.53 -1.3 4,596 - GFF7 0.53 +0.6 4,596 - GFF7 0.53 +0.2 4,596 - GFF7 0.53 -1.1 4,596 - GFF7 0.53 +0.2 4,605 - GFF7 0.55 -0.1 4,630 + GFF7 0.62 +1.4 4,640 + GFF7 0.64 +0.1 4,647 + GFF7 0.66 -2.5 4,647 + GFF7 0.66 -1.5 4,647 + GFF7 0.66 +0.0 4,654 + GFF7 0.68 -1.5 4,654 + GFF7 0.68 -0.8 4,662 - GFF7 0.70 -1.6 4,668 + GFF7 0.72 -0.7 4,668 + GFF7 0.72 +0.5 4,676 - GFF7 0.74 -0.6 4,676 - GFF7 0.74 -0.9 4,676 - GFF7 0.74 +0.8 4,678 + GFF7 0.74 -0.9 4,678 + GFF7 0.74 -2.0 4,678 + GFF7 0.74 -2.0 4,686 - GFF7 0.76 +0.4 4,686 - GFF7 0.76 +0.2 4,686 - GFF7 0.76 +0.4 4,686 - GFF7 0.76 +0.3 4,686 - GFF7 0.76 -0.2 4,686 - GFF7 0.76 +0.4 4,686 - GFF7 0.76 -0.4 4,686 - GFF7 0.76 -0.4 4,687 + GFF7 0.76 -0.3 4,696 + GFF7 0.79 +0.1 4,705 + GFF7 0.81 +0.5 4,711 - GFF7 0.83 +0.2 4,717 + GFF7 0.84 +0.5 4,721 + GFF7 0.85 -1.0 4,737 - GFF7 0.89 -0.1 4,737 - GFF7 0.89 +0.1 4,737 - GFF7 0.89 +0.4 4,739 + GFF7 0.90 +1.1 4,739 + GFF7 0.90 -0.1 4,742 - -0.1 4,747 + -1.7 4,747 - +0.3 4,747 - -1.9 4,747 - +0.7 4,747 - +0.8 4,757 - -0.8 4,793 + +0.5 4,801 - +0.2 4,858 - -0.4 4,858 - +0.9 4,872 + -1.2 4,888 - -1.4 4,968 + -1.5 5,014 - -3.0 5,080 + -3.3 5,124 - GFF8 0.16 -2.6 5,124 - GFF8 0.16 +1.0 5,148 - GFF8 0.22 +0.6 5,151 + GFF8 0.23 +0.3 5,151 + GFF8 0.23 -0.4 5,151 + GFF8 0.23 +0.9 5,154 - GFF8 0.23 +0.1 5,175 + GFF8 0.29 -0.2 5,176 + GFF8 0.29 -0.1 5,176 + GFF8 0.29 +0.8 5,179 - GFF8 0.30 -0.2 5,192 - GFF8 0.33 -0.0 5,209 + GFF8 0.37 -1.0 5,210 - GFF8 0.37 -0.4 5,212 + GFF8 0.38 -2.1 5,212 + GFF8 0.38 -0.4 5,266 - GFF8 0.51 +0.2 5,279 + GFF8 0.54 -0.6 5,281 - GFF8 0.55 -0.2 5,284 + GFF8 0.56 +0.7 5,295 + GFF8 0.58 -1.0 5,295 + GFF8 0.58 -1.2 5,303 - GFF8 0.60 -0.5 5,308 + GFF8 0.62 -0.8 5,343 + GFF8 0.70 +0.5 5,348 + GFF8 0.72 -1.2 5,348 + GFF8 0.72 -1.5 5,356 - GFF8 0.74 -1.5 5,392 + GFF8 0.83 -2.3 5,392 + GFF8 0.83 -0.2 5,392 + GFF8 0.83 +0.6 5,400 - GFF8 0.85 +0.4 5,407 + GFF8 0.86 -2.0 5,431 + -0.1 5,435 + -1.4 5,443 - -0.5 5,447 - -1.8 5,464 - -0.0 5,464 - -0.4 5,480 + +1.1 5,508 - -0.9 5,631 + GFF9 0.71 -1.2 5,634 - GFF9 0.74 +0.6 5,634 - GFF9 0.74 -0.9 5,636 + GFF9 0.75 -1.0 5,636 + GFF9 0.75 -0.3 5,636 + GFF9 0.75 +1.1 5,644 - GFF9 0.82 -0.1 5,663 - -1.6 5,686 - -0.1 5,696 + -1.1 5,696 + -0.4 5,702 - -1.2 5,702 - -0.2 5,704 - -0.0 5,712 - -1.2 5,714 - +0.5 5,729 - -1.0 5,746 + -1.9 5,746 + +0.5 5,747 + -0.7 5,754 - +0.8 5,757 - -1.0 5,760 + +1.6 5,763 - -2.1 5,770 - -1.2 5,777 - +0.7
Or see this region's nucleotide sequence