Experiment: EMM_noCarbon with Sucrose and Putrescine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2542 and GFF2543 are separated by 7 nucleotides GFF2543 and GFF2544 are separated by 143 nucleotides
GFF2542 - Sphingosine kinase and enzymes related to eukaryotic diacylglycerol kinase, at 2,559,640 to 2,560,605
GFF2542
GFF2543 - hypothetical protein, at 2,560,613 to 2,561,101
GFF2543
GFF2544 - Lipopolysaccharide export system permease protein LptF, at 2,561,245 to 2,562,456
GFF2544
Position (kb)
2560
2561
2562 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2559.626 kb on - strand at 2559.655 kb on - strand at 2559.739 kb on + strand, within GFF2542 at 2559.854 kb on - strand, within GFF2542 at 2560.620 kb on + strand at 2560.651 kb on - strand at 2560.651 kb on - strand at 2560.651 kb on - strand at 2560.654 kb on + strand at 2560.654 kb on + strand at 2560.659 kb on + strand at 2560.662 kb on - strand, within GFF2543 at 2560.667 kb on - strand, within GFF2543 at 2560.673 kb on + strand, within GFF2543 at 2560.680 kb on + strand, within GFF2543 at 2560.686 kb on + strand, within GFF2543 at 2560.686 kb on + strand, within GFF2543 at 2560.694 kb on - strand, within GFF2543 at 2560.696 kb on + strand, within GFF2543 at 2560.759 kb on + strand, within GFF2543 at 2560.769 kb on + strand, within GFF2543 at 2560.846 kb on - strand, within GFF2543 at 2560.855 kb on + strand, within GFF2543 at 2560.856 kb on - strand, within GFF2543 at 2560.860 kb on + strand, within GFF2543 at 2560.860 kb on - strand, within GFF2543 at 2560.868 kb on - strand, within GFF2543 at 2560.868 kb on - strand, within GFF2543 at 2560.892 kb on - strand, within GFF2543 at 2560.914 kb on + strand, within GFF2543 at 2560.914 kb on + strand, within GFF2543 at 2560.919 kb on + strand, within GFF2543 at 2560.948 kb on + strand, within GFF2543 at 2560.956 kb on - strand, within GFF2543 at 2560.956 kb on - strand, within GFF2543 at 2560.956 kb on - strand, within GFF2543 at 2560.956 kb on - strand, within GFF2543 at 2560.963 kb on - strand, within GFF2543 at 2560.967 kb on + strand, within GFF2543 at 2560.975 kb on - strand, within GFF2543 at 2560.998 kb on + strand, within GFF2543 at 2561.001 kb on + strand, within GFF2543 at 2561.001 kb on + strand, within GFF2543 at 2561.006 kb on - strand, within GFF2543 at 2561.011 kb on + strand, within GFF2543 at 2561.011 kb on - strand, within GFF2543 at 2561.014 kb on - strand, within GFF2543 at 2561.014 kb on - strand, within GFF2543 at 2561.015 kb on + strand, within GFF2543 at 2561.036 kb on + strand, within GFF2543 at 2561.044 kb on - strand, within GFF2543 at 2561.052 kb on + strand, within GFF2543 at 2561.052 kb on + strand, within GFF2543 at 2561.055 kb on - strand at 2561.065 kb on + strand at 2561.065 kb on - strand at 2561.075 kb on + strand at 2561.075 kb on + strand at 2561.099 kb on - strand at 2561.104 kb on + strand at 2561.104 kb on + strand at 2561.114 kb on + strand at 2561.122 kb on - strand at 2561.122 kb on - strand at 2561.129 kb on + strand at 2561.129 kb on - strand at 2561.156 kb on - strand at 2561.195 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction EMM_noCarbon with Sucrose and Putrescine remove 2,559,626 - +0.5 2,559,655 - +1.6 2,559,739 + GFF2542 0.10 -0.8 2,559,854 - GFF2542 0.22 -1.2 2,560,620 + -0.1 2,560,651 - +0.9 2,560,651 - +1.1 2,560,651 - -0.8 2,560,654 + +1.4 2,560,654 + -1.8 2,560,659 + +0.5 2,560,662 - GFF2543 0.10 +1.0 2,560,667 - GFF2543 0.11 +0.3 2,560,673 + GFF2543 0.12 -1.0 2,560,680 + GFF2543 0.14 +0.2 2,560,686 + GFF2543 0.15 +0.8 2,560,686 + GFF2543 0.15 +0.9 2,560,694 - GFF2543 0.17 +1.2 2,560,696 + GFF2543 0.17 +0.3 2,560,759 + GFF2543 0.30 -2.3 2,560,769 + GFF2543 0.32 +0.6 2,560,846 - GFF2543 0.48 -0.2 2,560,855 + GFF2543 0.49 +1.3 2,560,856 - GFF2543 0.50 +0.2 2,560,860 + GFF2543 0.51 -0.3 2,560,860 - GFF2543 0.51 -0.5 2,560,868 - GFF2543 0.52 -1.6 2,560,868 - GFF2543 0.52 +0.4 2,560,892 - GFF2543 0.57 +0.3 2,560,914 + GFF2543 0.62 -1.6 2,560,914 + GFF2543 0.62 -1.2 2,560,919 + GFF2543 0.63 +0.5 2,560,948 + GFF2543 0.69 +0.7 2,560,956 - GFF2543 0.70 -1.2 2,560,956 - GFF2543 0.70 +1.5 2,560,956 - GFF2543 0.70 -0.8 2,560,956 - GFF2543 0.70 -1.1 2,560,963 - GFF2543 0.72 +0.1 2,560,967 + GFF2543 0.72 -1.2 2,560,975 - GFF2543 0.74 -0.5 2,560,998 + GFF2543 0.79 -0.8 2,561,001 + GFF2543 0.79 +1.9 2,561,001 + GFF2543 0.79 +0.0 2,561,006 - GFF2543 0.80 -0.3 2,561,011 + GFF2543 0.81 +0.8 2,561,011 - GFF2543 0.81 -0.7 2,561,014 - GFF2543 0.82 +0.8 2,561,014 - GFF2543 0.82 -0.2 2,561,015 + GFF2543 0.82 +1.1 2,561,036 + GFF2543 0.87 -0.7 2,561,044 - GFF2543 0.88 -0.2 2,561,052 + GFF2543 0.90 +0.4 2,561,052 + GFF2543 0.90 -0.4 2,561,055 - -2.3 2,561,065 + +0.4 2,561,065 - -0.9 2,561,075 + +0.7 2,561,075 + -0.7 2,561,099 - -0.4 2,561,104 + +1.4 2,561,104 + -1.5 2,561,114 + +0.4 2,561,122 - -2.1 2,561,122 - -1.5 2,561,129 + +0.1 2,561,129 - +0.0 2,561,156 - +1.0 2,561,195 - -1.3
Or see this region's nucleotide sequence