Experiment: EMM_noCarbon with Sucrose and Putrescine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF142 and GFF143 are separated by 124 nucleotides GFF143 and GFF144 are separated by 6 nucleotides GFF144 and GFF145 are separated by 35 nucleotides
GFF142 - Polyhydroxyalkanoic acid synthase, at 137,081 to 138,055
GFF142
GFF143 - PhbF, at 138,180 to 138,758
GFF143
GFF144 - hypothetical protein, at 138,765 to 139,109
GFF144
GFF145 - Prolyl-tRNA synthetase (EC 6.1.1.15), archaeal/eukaryal type, at 139,145 to 140,725
GFF145
Position (kb)
138
139
140 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 137.803 kb on + strand, within GFF142 at 137.828 kb on - strand, within GFF142 at 137.834 kb on + strand, within GFF142 at 137.839 kb on + strand, within GFF142 at 137.839 kb on + strand, within GFF142 at 137.839 kb on + strand, within GFF142 at 137.839 kb on + strand, within GFF142 at 137.844 kb on - strand, within GFF142 at 137.847 kb on - strand, within GFF142 at 137.847 kb on - strand, within GFF142 at 137.860 kb on + strand, within GFF142 at 137.861 kb on + strand, within GFF142 at 137.861 kb on + strand, within GFF142 at 137.861 kb on + strand, within GFF142 at 137.868 kb on - strand, within GFF142 at 137.868 kb on - strand, within GFF142 at 137.869 kb on + strand, within GFF142 at 137.869 kb on - strand, within GFF142 at 137.869 kb on - strand, within GFF142 at 137.869 kb on - strand, within GFF142 at 137.869 kb on - strand, within GFF142 at 137.869 kb on - strand, within GFF142 at 137.869 kb on - strand, within GFF142 at 137.872 kb on + strand, within GFF142 at 137.872 kb on + strand, within GFF142 at 137.879 kb on + strand, within GFF142 at 137.880 kb on - strand, within GFF142 at 137.882 kb on + strand, within GFF142 at 137.893 kb on + strand, within GFF142 at 137.893 kb on + strand, within GFF142 at 137.894 kb on + strand, within GFF142 at 137.894 kb on + strand, within GFF142 at 137.931 kb on + strand, within GFF142 at 137.937 kb on + strand, within GFF142 at 137.953 kb on + strand, within GFF142 at 137.953 kb on + strand, within GFF142 at 137.953 kb on + strand, within GFF142 at 137.953 kb on + strand, within GFF142 at 137.953 kb on + strand, within GFF142 at 137.953 kb on + strand, within GFF142 at 137.953 kb on + strand, within GFF142 at 137.953 kb on + strand, within GFF142 at 137.953 kb on + strand, within GFF142 at 137.961 kb on + strand at 137.961 kb on - strand at 137.961 kb on - strand at 137.961 kb on - strand at 137.961 kb on - strand at 137.961 kb on - strand at 137.961 kb on - strand at 137.961 kb on - strand at 137.969 kb on - strand at 137.969 kb on - strand at 137.969 kb on - strand at 137.989 kb on + strand at 137.991 kb on - strand at 137.994 kb on + strand at 138.000 kb on + strand at 138.002 kb on - strand at 138.007 kb on - strand at 138.013 kb on + strand at 138.015 kb on + strand at 138.021 kb on - strand at 138.068 kb on - strand at 138.100 kb on + strand at 138.108 kb on - strand at 138.119 kb on + strand at 138.132 kb on + strand at 138.132 kb on + strand at 138.137 kb on + strand at 138.142 kb on + strand at 138.142 kb on + strand at 138.142 kb on + strand at 138.142 kb on + strand at 138.145 kb on - strand at 138.145 kb on - strand at 138.145 kb on - strand at 138.149 kb on + strand at 138.150 kb on - strand at 138.162 kb on + strand at 138.165 kb on - strand at 138.171 kb on - strand at 138.254 kb on - strand, within GFF143 at 138.340 kb on - strand, within GFF143 at 138.347 kb on + strand, within GFF143 at 138.355 kb on - strand, within GFF143 at 138.363 kb on + strand, within GFF143 at 138.363 kb on - strand, within GFF143 at 138.372 kb on - strand, within GFF143 at 138.464 kb on + strand, within GFF143 at 138.489 kb on - strand, within GFF143 at 138.497 kb on - strand, within GFF143 at 138.534 kb on + strand, within GFF143 at 138.534 kb on + strand, within GFF143 at 138.534 kb on + strand, within GFF143 at 138.542 kb on + strand, within GFF143 at 138.542 kb on - strand, within GFF143 at 138.542 kb on - strand, within GFF143 at 138.542 kb on - strand, within GFF143 at 138.566 kb on + strand, within GFF143 at 138.566 kb on - strand, within GFF143 at 138.579 kb on + strand, within GFF143 at 138.596 kb on + strand, within GFF143 at 138.604 kb on - strand, within GFF143 at 138.630 kb on - strand, within GFF143 at 138.646 kb on + strand, within GFF143 at 138.648 kb on + strand, within GFF143 at 138.666 kb on - strand, within GFF143 at 138.666 kb on - strand, within GFF143 at 138.666 kb on - strand, within GFF143 at 138.672 kb on - strand, within GFF143 at 138.752 kb on + strand at 138.767 kb on + strand at 138.770 kb on - strand at 138.781 kb on + strand at 138.781 kb on - strand at 138.791 kb on - strand at 138.851 kb on + strand, within GFF144 at 138.851 kb on + strand, within GFF144 at 138.859 kb on - strand, within GFF144 at 138.884 kb on - strand, within GFF144 at 138.889 kb on - strand, within GFF144 at 138.894 kb on - strand, within GFF144 at 138.896 kb on + strand, within GFF144 at 138.911 kb on + strand, within GFF144 at 138.919 kb on - strand, within GFF144 at 138.919 kb on - strand, within GFF144 at 138.936 kb on + strand, within GFF144 at 138.985 kb on + strand, within GFF144 at 138.985 kb on + strand, within GFF144 at 138.985 kb on + strand, within GFF144 at 138.994 kb on + strand, within GFF144 at 139.002 kb on - strand, within GFF144 at 139.023 kb on + strand, within GFF144 at 139.028 kb on + strand, within GFF144 at 139.028 kb on + strand, within GFF144 at 139.036 kb on - strand, within GFF144 at 139.036 kb on - strand, within GFF144 at 139.036 kb on - strand, within GFF144 at 139.057 kb on + strand, within GFF144 at 139.057 kb on + strand, within GFF144 at 139.065 kb on - strand, within GFF144 at 139.065 kb on - strand, within GFF144 at 139.067 kb on + strand, within GFF144 at 139.067 kb on + strand, within GFF144 at 139.067 kb on + strand, within GFF144 at 139.067 kb on + strand, within GFF144 at 139.067 kb on + strand, within GFF144 at 139.067 kb on + strand, within GFF144 at 139.067 kb on + strand, within GFF144 at 139.067 kb on + strand, within GFF144 at 139.067 kb on + strand, within GFF144 at 139.067 kb on + strand, within GFF144 at 139.067 kb on + strand, within GFF144 at 139.067 kb on + strand, within GFF144 at 139.067 kb on + strand, within GFF144 at 139.067 kb on + strand, within GFF144 at 139.067 kb on + strand, within GFF144 at 139.067 kb on + strand, within GFF144 at 139.070 kb on - strand, within GFF144 at 139.073 kb on + strand, within GFF144 at 139.075 kb on - strand at 139.075 kb on - strand at 139.075 kb on - strand at 139.075 kb on - strand at 139.075 kb on - strand at 139.075 kb on - strand at 139.075 kb on - strand at 139.075 kb on - strand at 139.075 kb on - strand at 139.075 kb on - strand at 139.075 kb on - strand at 139.075 kb on - strand at 139.081 kb on - strand at 139.081 kb on - strand at 139.113 kb on - strand at 139.120 kb on + strand at 139.128 kb on + strand at 139.135 kb on + strand at 139.135 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction EMM_noCarbon with Sucrose and Putrescine remove 137,803 + GFF142 0.74 -3.3 137,828 - GFF142 0.77 -1.7 137,834 + GFF142 0.77 -1.8 137,839 + GFF142 0.78 -2.7 137,839 + GFF142 0.78 -1.2 137,839 + GFF142 0.78 -2.0 137,839 + GFF142 0.78 -1.8 137,844 - GFF142 0.78 -2.0 137,847 - GFF142 0.79 -0.8 137,847 - GFF142 0.79 -4.0 137,860 + GFF142 0.80 -3.3 137,861 + GFF142 0.80 -0.4 137,861 + GFF142 0.80 -1.5 137,861 + GFF142 0.80 -0.9 137,868 - GFF142 0.81 -1.6 137,868 - GFF142 0.81 -0.7 137,869 + GFF142 0.81 -2.4 137,869 - GFF142 0.81 -1.4 137,869 - GFF142 0.81 -1.2 137,869 - GFF142 0.81 -1.3 137,869 - GFF142 0.81 -1.6 137,869 - GFF142 0.81 -3.1 137,869 - GFF142 0.81 -2.1 137,872 + GFF142 0.81 -1.4 137,872 + GFF142 0.81 -2.1 137,879 + GFF142 0.82 -1.4 137,880 - GFF142 0.82 -2.0 137,882 + GFF142 0.82 -2.0 137,893 + GFF142 0.83 -0.7 137,893 + GFF142 0.83 -1.6 137,894 + GFF142 0.83 -0.5 137,894 + GFF142 0.83 -1.7 137,931 + GFF142 0.87 -1.6 137,937 + GFF142 0.88 -2.7 137,953 + GFF142 0.89 -2.4 137,953 + GFF142 0.89 -0.8 137,953 + GFF142 0.89 -1.0 137,953 + GFF142 0.89 -1.4 137,953 + GFF142 0.89 -1.9 137,953 + GFF142 0.89 -1.6 137,953 + GFF142 0.89 -1.5 137,953 + GFF142 0.89 -1.6 137,953 + GFF142 0.89 -3.2 137,961 + -1.8 137,961 - -1.5 137,961 - -0.9 137,961 - -2.1 137,961 - -1.0 137,961 - -1.4 137,961 - -0.8 137,961 - -1.0 137,969 - -2.0 137,969 - -2.2 137,969 - -0.9 137,989 + -0.4 137,991 - -1.6 137,994 + -1.2 138,000 + -0.6 138,002 - -2.5 138,007 - -1.3 138,013 + -1.8 138,015 + +0.5 138,021 - -1.6 138,068 - -1.7 138,100 + -1.4 138,108 - -0.3 138,119 + -1.2 138,132 + -0.6 138,132 + +0.3 138,137 + -2.1 138,142 + -1.2 138,142 + -1.4 138,142 + -1.6 138,142 + -1.8 138,145 - -0.2 138,145 - -0.4 138,145 - -0.2 138,149 + -1.2 138,150 - -1.4 138,162 + -2.5 138,165 - -2.2 138,171 - -1.8 138,254 - GFF143 0.13 -2.5 138,340 - GFF143 0.28 +0.5 138,347 + GFF143 0.29 +0.3 138,355 - GFF143 0.30 -2.3 138,363 + GFF143 0.32 -0.6 138,363 - GFF143 0.32 +0.5 138,372 - GFF143 0.33 -1.9 138,464 + GFF143 0.49 -2.0 138,489 - GFF143 0.53 +0.6 138,497 - GFF143 0.55 +0.7 138,534 + GFF143 0.61 -0.0 138,534 + GFF143 0.61 -1.7 138,534 + GFF143 0.61 -1.0 138,542 + GFF143 0.63 +0.5 138,542 - GFF143 0.63 -1.2 138,542 - GFF143 0.63 +1.0 138,542 - GFF143 0.63 -1.6 138,566 + GFF143 0.67 -0.7 138,566 - GFF143 0.67 -1.5 138,579 + GFF143 0.69 +0.0 138,596 + GFF143 0.72 -1.7 138,604 - GFF143 0.73 -0.4 138,630 - GFF143 0.78 +0.1 138,646 + GFF143 0.80 +0.4 138,648 + GFF143 0.81 -1.1 138,666 - GFF143 0.84 -1.0 138,666 - GFF143 0.84 -2.5 138,666 - GFF143 0.84 +0.3 138,672 - GFF143 0.85 -1.4 138,752 + +0.2 138,767 + -0.4 138,770 - -2.1 138,781 + +0.3 138,781 - +0.6 138,791 - -1.5 138,851 + GFF144 0.25 -1.6 138,851 + GFF144 0.25 -0.8 138,859 - GFF144 0.27 -0.4 138,884 - GFF144 0.34 +0.7 138,889 - GFF144 0.36 +0.1 138,894 - GFF144 0.37 +0.9 138,896 + GFF144 0.38 -1.1 138,911 + GFF144 0.42 +1.7 138,919 - GFF144 0.45 -1.4 138,919 - GFF144 0.45 -1.2 138,936 + GFF144 0.50 +0.6 138,985 + GFF144 0.64 +0.3 138,985 + GFF144 0.64 +0.4 138,985 + GFF144 0.64 +0.1 138,994 + GFF144 0.66 -0.5 139,002 - GFF144 0.69 +0.3 139,023 + GFF144 0.75 +0.1 139,028 + GFF144 0.76 -2.6 139,028 + GFF144 0.76 -1.6 139,036 - GFF144 0.79 -0.4 139,036 - GFF144 0.79 -0.5 139,036 - GFF144 0.79 -0.7 139,057 + GFF144 0.85 -1.5 139,057 + GFF144 0.85 -1.6 139,065 - GFF144 0.87 +0.6 139,065 - GFF144 0.87 -0.2 139,067 + GFF144 0.88 +0.3 139,067 + GFF144 0.88 +0.2 139,067 + GFF144 0.88 -0.1 139,067 + GFF144 0.88 -1.7 139,067 + GFF144 0.88 +0.1 139,067 + GFF144 0.88 -1.1 139,067 + GFF144 0.88 +0.8 139,067 + GFF144 0.88 -1.5 139,067 + GFF144 0.88 -0.9 139,067 + GFF144 0.88 +0.2 139,067 + GFF144 0.88 -1.4 139,067 + GFF144 0.88 -1.4 139,067 + GFF144 0.88 +1.3 139,067 + GFF144 0.88 -1.4 139,067 + GFF144 0.88 -0.2 139,067 + GFF144 0.88 +0.4 139,070 - GFF144 0.88 -2.0 139,073 + GFF144 0.89 +0.3 139,075 - -0.4 139,075 - -0.8 139,075 - +0.1 139,075 - -0.8 139,075 - +0.1 139,075 - -0.1 139,075 - +0.5 139,075 - +0.6 139,075 - -0.2 139,075 - -1.2 139,075 - -0.0 139,075 - -0.1 139,081 - -0.7 139,081 - -1.1 139,113 - -0.7 139,120 + +0.8 139,128 + -0.0 139,135 + +0.3 139,135 - +0.1
Or see this region's nucleotide sequence