Experiment: EMM_noCarbon with Sucrose and Putrescine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF967 and GFF968 are separated by 24 nucleotides GFF968 and GFF969 overlap by 1 nucleotides GFF969 and GFF970 overlap by 4 nucleotides GFF970 and GFF971 overlap by 4 nucleotides GFF971 and GFF972 overlap by 4 nucleotides
GFF967 - Nucleoside-diphosphate-sugar epimerases, at 968,547 to 969,362
GFF967
GFF968 - Iron-sulfur cluster assembly iron binding protein SufA, at 969,387 to 969,749
GFF968
GFF969 - PaaD-like protein (DUF59) involved in Fe-S cluster assembly, at 969,749 to 970,210
GFF969
GFF970 - Cysteine desulfurase (EC 2.8.1.7) => SufS, at 970,207 to 971,412
GFF970
GFF971 - Iron-sulfur cluster assembly protein SufD, at 971,409 to 972,161
GFF971
GFF972 - Iron-sulfur cluster assembly ATPase protein SufC, at 972,158 to 972,904
GFF972
Position (kb)
970
971
972 Strain fitness (log2 ratio)
-2
-1
0
1 at 969.224 kb on + strand, within GFF967 at 969.262 kb on - strand, within GFF967 at 969.275 kb on + strand, within GFF967 at 969.277 kb on + strand, within GFF967 at 969.298 kb on + strand at 969.306 kb on - strand at 969.325 kb on - strand at 969.328 kb on + strand at 969.335 kb on - strand at 969.336 kb on - strand at 969.340 kb on - strand at 969.345 kb on - strand at 969.352 kb on - strand at 969.352 kb on - strand at 969.357 kb on - strand at 969.357 kb on - strand at 969.381 kb on - strand at 969.381 kb on - strand at 969.384 kb on + strand at 969.384 kb on + strand at 969.392 kb on - strand at 969.541 kb on + strand, within GFF968 at 969.960 kb on - strand, within GFF969 at 971.415 kb on - strand at 971.415 kb on - strand at 971.427 kb on - strand at 971.427 kb on - strand at 971.427 kb on - strand at 972.180 kb on - strand at 972.180 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction EMM_noCarbon with Sucrose and Putrescine remove 969,224 + GFF967 0.83 -1.7 969,262 - GFF967 0.88 -1.1 969,275 + GFF967 0.89 -1.0 969,277 + GFF967 0.89 -0.9 969,298 + +0.1 969,306 - -0.9 969,325 - +0.7 969,328 + -0.3 969,335 - -0.3 969,336 - +0.0 969,340 - -1.0 969,345 - +1.1 969,352 - +1.0 969,352 - -0.3 969,357 - -0.5 969,357 - +1.2 969,381 - +0.8 969,381 - +0.6 969,384 + -0.5 969,384 + -0.7 969,392 - -0.1 969,541 + GFF968 0.42 -1.1 969,960 - GFF969 0.46 -1.7 971,415 - +0.4 971,415 - -0.4 971,427 - -1.1 971,427 - -1.0 971,427 - -0.8 972,180 - -0.3 972,180 - -1.8
Or see this region's nucleotide sequence