Experiment: EMM_noCarbon with Sucrose and Putrescine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF966 and GFF967 are separated by 77 nucleotides GFF967 and GFF968 are separated by 24 nucleotides GFF968 and GFF969 overlap by 1 nucleotides GFF969 and GFF970 overlap by 4 nucleotides
GFF966 - hypothetical protein, at 968,311 to 968,469
GFF966
GFF967 - Nucleoside-diphosphate-sugar epimerases, at 968,547 to 969,362
GFF967
GFF968 - Iron-sulfur cluster assembly iron binding protein SufA, at 969,387 to 969,749
GFF968
GFF969 - PaaD-like protein (DUF59) involved in Fe-S cluster assembly, at 969,749 to 970,210
GFF969
GFF970 - Cysteine desulfurase (EC 2.8.1.7) => SufS, at 970,207 to 971,412
GFF970
Position (kb)
969
970 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 968.416 kb on - strand, within GFF966 at 968.420 kb on + strand, within GFF966 at 968.420 kb on + strand, within GFF966 at 968.420 kb on + strand, within GFF966 at 968.428 kb on - strand, within GFF966 at 968.428 kb on - strand, within GFF966 at 968.428 kb on - strand, within GFF966 at 968.439 kb on - strand, within GFF966 at 968.440 kb on + strand, within GFF966 at 968.458 kb on - strand at 968.462 kb on - strand at 968.464 kb on + strand at 968.464 kb on + strand at 968.464 kb on + strand at 968.464 kb on + strand at 968.472 kb on - strand at 968.472 kb on - strand at 968.472 kb on - strand at 968.472 kb on - strand at 968.472 kb on - strand at 968.472 kb on - strand at 968.484 kb on - strand at 968.484 kb on - strand at 968.484 kb on - strand at 968.496 kb on - strand at 968.512 kb on + strand at 968.514 kb on + strand at 968.514 kb on + strand at 968.514 kb on + strand at 968.522 kb on - strand at 968.522 kb on - strand at 968.522 kb on - strand at 968.522 kb on - strand at 968.522 kb on - strand at 968.522 kb on - strand at 968.522 kb on - strand at 968.522 kb on - strand at 968.522 kb on - strand at 968.532 kb on - strand at 968.579 kb on + strand at 968.580 kb on + strand at 968.580 kb on + strand at 968.587 kb on - strand at 968.587 kb on - strand at 968.587 kb on - strand at 968.587 kb on - strand at 968.587 kb on - strand at 968.587 kb on - strand at 968.605 kb on - strand at 968.610 kb on - strand at 968.611 kb on + strand at 968.612 kb on + strand at 968.612 kb on + strand at 968.620 kb on - strand at 968.622 kb on + strand at 968.625 kb on - strand at 968.630 kb on - strand, within GFF967 at 968.648 kb on - strand, within GFF967 at 968.678 kb on - strand, within GFF967 at 968.685 kb on + strand, within GFF967 at 968.687 kb on - strand, within GFF967 at 968.694 kb on - strand, within GFF967 at 968.702 kb on + strand, within GFF967 at 968.710 kb on - strand, within GFF967 at 968.710 kb on - strand, within GFF967 at 968.710 kb on - strand, within GFF967 at 968.733 kb on + strand, within GFF967 at 968.733 kb on + strand, within GFF967 at 968.733 kb on + strand, within GFF967 at 968.733 kb on + strand, within GFF967 at 968.736 kb on - strand, within GFF967 at 968.741 kb on - strand, within GFF967 at 968.741 kb on - strand, within GFF967 at 968.765 kb on + strand, within GFF967 at 968.769 kb on - strand, within GFF967 at 968.795 kb on + strand, within GFF967 at 968.795 kb on + strand, within GFF967 at 968.803 kb on - strand, within GFF967 at 968.803 kb on - strand, within GFF967 at 968.803 kb on - strand, within GFF967 at 968.803 kb on - strand, within GFF967 at 968.865 kb on + strand, within GFF967 at 968.865 kb on + strand, within GFF967 at 968.865 kb on + strand, within GFF967 at 968.865 kb on + strand, within GFF967 at 968.873 kb on - strand, within GFF967 at 968.873 kb on - strand, within GFF967 at 968.873 kb on - strand, within GFF967 at 968.873 kb on - strand, within GFF967 at 968.875 kb on + strand, within GFF967 at 968.908 kb on + strand, within GFF967 at 968.908 kb on + strand, within GFF967 at 968.938 kb on + strand, within GFF967 at 968.938 kb on + strand, within GFF967 at 968.938 kb on + strand, within GFF967 at 968.941 kb on - strand, within GFF967 at 968.946 kb on - strand, within GFF967 at 968.946 kb on - strand, within GFF967 at 968.946 kb on - strand, within GFF967 at 968.946 kb on - strand, within GFF967 at 968.946 kb on - strand, within GFF967 at 968.965 kb on - strand, within GFF967 at 969.017 kb on - strand, within GFF967 at 969.027 kb on + strand, within GFF967 at 969.032 kb on + strand, within GFF967 at 969.035 kb on + strand, within GFF967 at 969.035 kb on - strand, within GFF967 at 969.035 kb on - strand, within GFF967 at 969.035 kb on - strand, within GFF967 at 969.035 kb on - strand, within GFF967 at 969.038 kb on - strand, within GFF967 at 969.040 kb on - strand, within GFF967 at 969.040 kb on - strand, within GFF967 at 969.048 kb on - strand, within GFF967 at 969.075 kb on + strand, within GFF967 at 969.088 kb on + strand, within GFF967 at 969.088 kb on + strand, within GFF967 at 969.096 kb on - strand, within GFF967 at 969.104 kb on - strand, within GFF967 at 969.109 kb on - strand, within GFF967 at 969.152 kb on + strand, within GFF967 at 969.160 kb on - strand, within GFF967 at 969.160 kb on - strand, within GFF967 at 969.169 kb on + strand, within GFF967 at 969.169 kb on + strand, within GFF967 at 969.170 kb on - strand, within GFF967 at 969.179 kb on + strand, within GFF967 at 969.179 kb on + strand, within GFF967 at 969.179 kb on + strand, within GFF967 at 969.184 kb on + strand, within GFF967 at 969.187 kb on - strand, within GFF967 at 969.187 kb on - strand, within GFF967 at 969.187 kb on - strand, within GFF967 at 969.191 kb on + strand, within GFF967 at 969.191 kb on + strand, within GFF967 at 969.191 kb on + strand, within GFF967 at 969.192 kb on + strand, within GFF967 at 969.192 kb on + strand, within GFF967 at 969.192 kb on + strand, within GFF967 at 969.197 kb on + strand, within GFF967 at 969.199 kb on - strand, within GFF967 at 969.199 kb on - strand, within GFF967 at 969.200 kb on - strand, within GFF967 at 969.224 kb on + strand, within GFF967 at 969.262 kb on - strand, within GFF967 at 969.275 kb on + strand, within GFF967 at 969.277 kb on + strand, within GFF967 at 969.298 kb on + strand at 969.306 kb on - strand at 969.325 kb on - strand at 969.328 kb on + strand at 969.335 kb on - strand at 969.336 kb on - strand at 969.340 kb on - strand at 969.345 kb on - strand at 969.352 kb on - strand at 969.352 kb on - strand at 969.357 kb on - strand at 969.357 kb on - strand at 969.381 kb on - strand at 969.381 kb on - strand at 969.384 kb on + strand at 969.384 kb on + strand at 969.392 kb on - strand at 969.541 kb on + strand, within GFF968 at 969.960 kb on - strand, within GFF969
Per-strain Table
Position Strand Gene LocusTag Fraction EMM_noCarbon with Sucrose and Putrescine remove 968,416 - GFF966 0.66 +0.4 968,420 + GFF966 0.69 +0.2 968,420 + GFF966 0.69 +0.5 968,420 + GFF966 0.69 +0.4 968,428 - GFF966 0.74 +1.2 968,428 - GFF966 0.74 -1.1 968,428 - GFF966 0.74 -1.5 968,439 - GFF966 0.81 -0.3 968,440 + GFF966 0.81 +0.8 968,458 - +1.4 968,462 - -1.1 968,464 + +0.0 968,464 + -0.5 968,464 + +0.5 968,464 + +0.0 968,472 - -1.2 968,472 - -0.8 968,472 - +0.7 968,472 - +0.5 968,472 - -0.6 968,472 - -1.0 968,484 - +0.3 968,484 - -1.7 968,484 - -1.4 968,496 - -0.5 968,512 + -0.2 968,514 + -0.3 968,514 + +0.1 968,514 + +0.5 968,522 - -0.7 968,522 - -1.2 968,522 - +0.1 968,522 - -0.4 968,522 - +0.1 968,522 - -0.4 968,522 - +0.8 968,522 - -1.0 968,522 - -2.1 968,532 - -1.0 968,579 + -0.5 968,580 + -1.3 968,580 + -1.1 968,587 - -0.7 968,587 - -1.0 968,587 - -0.6 968,587 - -1.5 968,587 - +0.5 968,587 - -0.0 968,605 - -0.4 968,610 - -0.1 968,611 + -1.0 968,612 + -1.9 968,612 + +0.3 968,620 - -1.1 968,622 + +0.9 968,625 - -0.7 968,630 - GFF967 0.10 +1.1 968,648 - GFF967 0.12 +0.9 968,678 - GFF967 0.16 -2.2 968,685 + GFF967 0.17 -1.1 968,687 - GFF967 0.17 -0.5 968,694 - GFF967 0.18 -0.9 968,702 + GFF967 0.19 -2.4 968,710 - GFF967 0.20 +1.3 968,710 - GFF967 0.20 +2.0 968,710 - GFF967 0.20 -1.0 968,733 + GFF967 0.23 -0.2 968,733 + GFF967 0.23 -2.2 968,733 + GFF967 0.23 +0.3 968,733 + GFF967 0.23 -1.2 968,736 - GFF967 0.23 -0.9 968,741 - GFF967 0.24 -0.2 968,741 - GFF967 0.24 +0.7 968,765 + GFF967 0.27 -0.6 968,769 - GFF967 0.27 -0.6 968,795 + GFF967 0.30 -0.9 968,795 + GFF967 0.30 -0.4 968,803 - GFF967 0.31 +1.5 968,803 - GFF967 0.31 +0.1 968,803 - GFF967 0.31 +0.2 968,803 - GFF967 0.31 +0.8 968,865 + GFF967 0.39 -3.1 968,865 + GFF967 0.39 -1.3 968,865 + GFF967 0.39 +0.3 968,865 + GFF967 0.39 -1.4 968,873 - GFF967 0.40 +0.9 968,873 - GFF967 0.40 +0.6 968,873 - GFF967 0.40 -0.7 968,873 - GFF967 0.40 -1.0 968,875 + GFF967 0.40 +0.9 968,908 + GFF967 0.44 +0.4 968,908 + GFF967 0.44 +0.4 968,938 + GFF967 0.48 -0.3 968,938 + GFF967 0.48 -2.0 968,938 + GFF967 0.48 -1.6 968,941 - GFF967 0.48 -1.5 968,946 - GFF967 0.49 -0.4 968,946 - GFF967 0.49 -1.2 968,946 - GFF967 0.49 -2.9 968,946 - GFF967 0.49 +0.1 968,946 - GFF967 0.49 -0.2 968,965 - GFF967 0.51 +1.5 969,017 - GFF967 0.58 +0.4 969,027 + GFF967 0.59 +0.2 969,032 + GFF967 0.59 -0.2 969,035 + GFF967 0.60 +0.0 969,035 - GFF967 0.60 -0.3 969,035 - GFF967 0.60 +0.6 969,035 - GFF967 0.60 -0.6 969,035 - GFF967 0.60 -0.3 969,038 - GFF967 0.60 +0.6 969,040 - GFF967 0.60 -0.2 969,040 - GFF967 0.60 +1.6 969,048 - GFF967 0.61 +0.9 969,075 + GFF967 0.65 -2.5 969,088 + GFF967 0.66 -0.4 969,088 + GFF967 0.66 -0.4 969,096 - GFF967 0.67 -0.2 969,104 - GFF967 0.68 -2.3 969,109 - GFF967 0.69 -1.2 969,152 + GFF967 0.74 -1.4 969,160 - GFF967 0.75 -0.3 969,160 - GFF967 0.75 -0.3 969,169 + GFF967 0.76 +0.2 969,169 + GFF967 0.76 +0.4 969,170 - GFF967 0.76 -0.8 969,179 + GFF967 0.77 -1.8 969,179 + GFF967 0.77 -1.3 969,179 + GFF967 0.77 +0.4 969,184 + GFF967 0.78 -2.8 969,187 - GFF967 0.78 -0.7 969,187 - GFF967 0.78 -1.5 969,187 - GFF967 0.78 +0.5 969,191 + GFF967 0.79 -0.1 969,191 + GFF967 0.79 +0.2 969,191 + GFF967 0.79 -1.2 969,192 + GFF967 0.79 -0.5 969,192 + GFF967 0.79 +0.6 969,192 + GFF967 0.79 +0.6 969,197 + GFF967 0.80 +0.5 969,199 - GFF967 0.80 -0.1 969,199 - GFF967 0.80 -0.2 969,200 - GFF967 0.80 -1.2 969,224 + GFF967 0.83 -1.7 969,262 - GFF967 0.88 -1.1 969,275 + GFF967 0.89 -1.0 969,277 + GFF967 0.89 -0.9 969,298 + +0.1 969,306 - -0.9 969,325 - +0.7 969,328 + -0.3 969,335 - -0.3 969,336 - +0.0 969,340 - -1.0 969,345 - +1.1 969,352 - +1.0 969,352 - -0.3 969,357 - -0.5 969,357 - +1.2 969,381 - +0.8 969,381 - +0.6 969,384 + -0.5 969,384 + -0.7 969,392 - -0.1 969,541 + GFF968 0.42 -1.1 969,960 - GFF969 0.46 -1.7
Or see this region's nucleotide sequence