Experiment: EMM_noCarbon with Sucrose and Putrescine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF822 and GFF823 are separated by 28 nucleotides GFF823 and GFF824 overlap by 4 nucleotides GFF824 and GFF825 overlap by 8 nucleotides GFF825 and GFF826 overlap by 4 nucleotides
GFF822 - 2,3,4,5-tetrahydropyridine-2,6-dicarboxylate N-succinyltransferase (EC 2.3.1.117), at 811,277 to 812,116
GFF822
GFF823 - Adenosylcobinamide kinase (EC 2.7.1.156) / Adenosylcobinamide-phosphate guanylyltransferase (EC 2.7.7.62), at 812,145 to 812,651
GFF823
GFF824 - Adenosylcobinamide-phosphate synthase (EC 6.3.1.10), at 812,648 to 813,601
GFF824
GFF825 - L-threonine 3-O-phosphate decarboxylase (EC 4.1.1.81), at 813,594 to 814,577
GFF825
GFF826 - Cobalamin synthase (EC 2.7.8.26), at 814,574 to 815,293
GFF826
Position (kb) 812
813
814 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 812.120 kb on - strand at 812.120 kb on - strand at 812.135 kb on - strand at 812.142 kb on + strand at 812.142 kb on + strand at 812.142 kb on + strand at 812.148 kb on + strand at 812.151 kb on + strand at 812.158 kb on - strand at 812.224 kb on + strand, within GFF823 at 812.224 kb on - strand, within GFF823 at 812.232 kb on - strand, within GFF823 at 812.282 kb on - strand, within GFF823 at 812.301 kb on + strand, within GFF823 at 812.369 kb on - strand, within GFF823 at 812.396 kb on + strand, within GFF823 at 812.419 kb on + strand, within GFF823 at 812.419 kb on + strand, within GFF823 at 812.419 kb on + strand, within GFF823 at 812.419 kb on + strand, within GFF823 at 812.419 kb on + strand, within GFF823 at 812.419 kb on + strand, within GFF823 at 812.419 kb on + strand, within GFF823 at 812.422 kb on - strand, within GFF823 at 812.427 kb on - strand, within GFF823 at 812.427 kb on - strand, within GFF823 at 812.427 kb on - strand, within GFF823 at 812.427 kb on - strand, within GFF823 at 812.427 kb on - strand, within GFF823 at 812.427 kb on - strand, within GFF823 at 812.427 kb on - strand, within GFF823 at 812.427 kb on - strand, within GFF823 at 812.427 kb on - strand, within GFF823 at 812.427 kb on - strand, within GFF823 at 812.427 kb on - strand, within GFF823 at 812.480 kb on + strand, within GFF823 at 812.497 kb on + strand, within GFF823 at 812.502 kb on + strand, within GFF823 at 812.509 kb on - strand, within GFF823 at 812.554 kb on + strand, within GFF823 at 812.562 kb on - strand, within GFF823 at 812.571 kb on + strand, within GFF823 at 812.602 kb on - strand at 812.633 kb on - strand at 812.651 kb on + strand at 812.652 kb on + strand at 812.659 kb on + strand at 812.691 kb on + strand at 812.691 kb on + strand at 812.691 kb on + strand at 812.694 kb on - strand at 812.696 kb on + strand at 812.704 kb on - strand at 812.708 kb on + strand at 812.708 kb on + strand at 812.710 kb on + strand at 812.716 kb on - strand at 812.716 kb on - strand at 812.716 kb on - strand at 812.754 kb on + strand, within GFF824 at 812.782 kb on + strand, within GFF824 at 812.805 kb on - strand, within GFF824 at 812.805 kb on - strand, within GFF824 at 812.805 kb on - strand, within GFF824 at 812.824 kb on + strand, within GFF824 at 812.838 kb on - strand, within GFF824 at 812.838 kb on - strand, within GFF824 at 812.886 kb on - strand, within GFF824 at 812.886 kb on - strand, within GFF824 at 812.922 kb on + strand, within GFF824 at 812.936 kb on + strand, within GFF824 at 812.936 kb on + strand, within GFF824 at 812.977 kb on + strand, within GFF824 at 812.985 kb on - strand, within GFF824 at 812.985 kb on - strand, within GFF824 at 813.036 kb on + strand, within GFF824 at 813.061 kb on - strand, within GFF824 at 813.076 kb on - strand, within GFF824 at 813.085 kb on + strand, within GFF824 at 813.110 kb on + strand, within GFF824 at 813.139 kb on + strand, within GFF824 at 813.164 kb on + strand, within GFF824 at 813.213 kb on - strand, within GFF824 at 813.276 kb on + strand, within GFF824 at 813.276 kb on + strand, within GFF824 at 813.279 kb on + strand, within GFF824 at 813.311 kb on + strand, within GFF824 at 813.314 kb on - strand, within GFF824 at 813.319 kb on - strand, within GFF824 at 813.368 kb on + strand, within GFF824 at 813.499 kb on + strand, within GFF824 at 813.507 kb on - strand at 813.559 kb on - strand at 813.619 kb on + strand at 813.619 kb on + strand at 813.624 kb on + strand at 813.624 kb on + strand at 813.624 kb on + strand at 813.624 kb on + strand at 813.624 kb on + strand at 813.632 kb on - strand at 813.658 kb on - strand at 813.671 kb on + strand at 813.680 kb on + strand at 813.698 kb on - strand, within GFF825 at 813.699 kb on + strand, within GFF825 at 813.733 kb on - strand, within GFF825 at 813.779 kb on + strand, within GFF825 at 813.779 kb on + strand, within GFF825 at 813.787 kb on - strand, within GFF825 at 813.787 kb on - strand, within GFF825 at 813.853 kb on + strand, within GFF825 at 813.853 kb on + strand, within GFF825 at 813.856 kb on - strand, within GFF825 at 813.911 kb on + strand, within GFF825 at 813.913 kb on - strand, within GFF825 at 813.917 kb on + strand, within GFF825 at 813.927 kb on + strand, within GFF825 at 813.930 kb on - strand, within GFF825 at 813.970 kb on - strand, within GFF825 at 814.047 kb on - strand, within GFF825 at 814.047 kb on - strand, within GFF825 at 814.083 kb on + strand, within GFF825 at 814.091 kb on - strand, within GFF825 at 814.094 kb on + strand, within GFF825 at 814.114 kb on - strand, within GFF825 at 814.148 kb on + strand, within GFF825 at 814.156 kb on - strand, within GFF825 at 814.156 kb on - strand, within GFF825 at 814.188 kb on + strand, within GFF825 at 814.188 kb on + strand, within GFF825 at 814.188 kb on + strand, within GFF825 at 814.193 kb on + strand, within GFF825 at 814.193 kb on + strand, within GFF825 at 814.195 kb on - strand, within GFF825 at 814.196 kb on - strand, within GFF825 at 814.207 kb on + strand, within GFF825 at 814.207 kb on + strand, within GFF825 at 814.211 kb on + strand, within GFF825 at 814.211 kb on + strand, within GFF825 at 814.221 kb on + strand, within GFF825 at 814.242 kb on + strand, within GFF825 at 814.250 kb on - strand, within GFF825 at 814.289 kb on + strand, within GFF825 at 814.325 kb on + strand, within GFF825 at 814.336 kb on + strand, within GFF825 at 814.338 kb on + strand, within GFF825 at 814.346 kb on - strand, within GFF825 at 814.348 kb on + strand, within GFF825 at 814.356 kb on - strand, within GFF825 at 814.384 kb on + strand, within GFF825 at 814.384 kb on + strand, within GFF825 at 814.392 kb on - strand, within GFF825 at 814.467 kb on + strand, within GFF825 at 814.469 kb on - strand, within GFF825 at 814.470 kb on - strand, within GFF825 at 814.482 kb on - strand at 814.501 kb on - strand at 814.503 kb on + strand at 814.511 kb on - strand at 814.573 kb on + strand at 814.573 kb on + strand at 814.579 kb on - strand at 814.595 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction EMM_noCarbon with Sucrose and Putrescine remove 812,120 - +0.8 812,120 - -0.1 812,135 - +0.2 812,142 + +0.5 812,142 + -0.8 812,142 + -1.6 812,148 + -2.9 812,151 + -1.8 812,158 - -0.4 812,224 + GFF823 0.16 -1.4 812,224 - GFF823 0.16 -1.3 812,232 - GFF823 0.17 -1.8 812,282 - GFF823 0.27 -2.1 812,301 + GFF823 0.31 -2.5 812,369 - GFF823 0.44 -1.8 812,396 + GFF823 0.50 -1.3 812,419 + GFF823 0.54 -2.4 812,419 + GFF823 0.54 -0.5 812,419 + GFF823 0.54 -1.7 812,419 + GFF823 0.54 -1.5 812,419 + GFF823 0.54 -2.7 812,419 + GFF823 0.54 -1.5 812,419 + GFF823 0.54 -1.1 812,422 - GFF823 0.55 -2.2 812,427 - GFF823 0.56 -1.1 812,427 - GFF823 0.56 -1.5 812,427 - GFF823 0.56 -1.2 812,427 - GFF823 0.56 -2.5 812,427 - GFF823 0.56 -1.5 812,427 - GFF823 0.56 -2.6 812,427 - GFF823 0.56 -1.8 812,427 - GFF823 0.56 -1.7 812,427 - GFF823 0.56 -2.2 812,427 - GFF823 0.56 -2.1 812,427 - GFF823 0.56 -2.9 812,480 + GFF823 0.66 -1.4 812,497 + GFF823 0.69 -1.2 812,502 + GFF823 0.70 -1.3 812,509 - GFF823 0.72 -1.6 812,554 + GFF823 0.81 -1.2 812,562 - GFF823 0.82 -1.9 812,571 + GFF823 0.84 -2.5 812,602 - -2.3 812,633 - -1.7 812,651 + -0.5 812,652 + -2.2 812,659 + +0.3 812,691 + -0.5 812,691 + -2.8 812,691 + +1.3 812,694 - -1.4 812,696 + -0.2 812,704 - -0.1 812,708 + -2.4 812,708 + -1.3 812,710 + -0.2 812,716 - +0.0 812,716 - -0.0 812,716 - -0.9 812,754 + GFF824 0.11 -1.8 812,782 + GFF824 0.14 -2.3 812,805 - GFF824 0.16 -1.2 812,805 - GFF824 0.16 -1.1 812,805 - GFF824 0.16 -1.9 812,824 + GFF824 0.18 -1.6 812,838 - GFF824 0.20 -1.8 812,838 - GFF824 0.20 -3.2 812,886 - GFF824 0.25 -1.8 812,886 - GFF824 0.25 -1.3 812,922 + GFF824 0.29 -0.6 812,936 + GFF824 0.30 -1.4 812,936 + GFF824 0.30 -1.5 812,977 + GFF824 0.34 -1.6 812,985 - GFF824 0.35 -1.6 812,985 - GFF824 0.35 -1.7 813,036 + GFF824 0.41 -1.8 813,061 - GFF824 0.43 -1.5 813,076 - GFF824 0.45 -1.4 813,085 + GFF824 0.46 -2.5 813,110 + GFF824 0.48 -1.3 813,139 + GFF824 0.51 -1.7 813,164 + GFF824 0.54 -1.7 813,213 - GFF824 0.59 -1.4 813,276 + GFF824 0.66 -2.2 813,276 + GFF824 0.66 -1.1 813,279 + GFF824 0.66 -1.5 813,311 + GFF824 0.69 +0.9 813,314 - GFF824 0.70 +0.5 813,319 - GFF824 0.70 -1.3 813,368 + GFF824 0.75 -2.5 813,499 + GFF824 0.89 -1.1 813,507 - -1.3 813,559 - -0.2 813,619 + -0.3 813,619 + -1.8 813,624 + -1.8 813,624 + -2.8 813,624 + -1.8 813,624 + -2.6 813,624 + -2.5 813,632 - -0.5 813,658 - -1.5 813,671 + -1.2 813,680 + -1.6 813,698 - GFF825 0.11 -1.4 813,699 + GFF825 0.11 -1.8 813,733 - GFF825 0.14 -2.3 813,779 + GFF825 0.19 -1.4 813,779 + GFF825 0.19 -0.9 813,787 - GFF825 0.20 -1.7 813,787 - GFF825 0.20 -1.0 813,853 + GFF825 0.26 -1.2 813,853 + GFF825 0.26 -2.4 813,856 - GFF825 0.27 -1.1 813,911 + GFF825 0.32 -2.6 813,913 - GFF825 0.32 -1.2 813,917 + GFF825 0.33 -1.9 813,927 + GFF825 0.34 -1.4 813,930 - GFF825 0.34 -2.1 813,970 - GFF825 0.38 -0.2 814,047 - GFF825 0.46 -1.1 814,047 - GFF825 0.46 -1.8 814,083 + GFF825 0.50 -0.9 814,091 - GFF825 0.51 -0.1 814,094 + GFF825 0.51 -1.5 814,114 - GFF825 0.53 -1.4 814,148 + GFF825 0.56 -1.9 814,156 - GFF825 0.57 -1.2 814,156 - GFF825 0.57 -1.7 814,188 + GFF825 0.60 -1.2 814,188 + GFF825 0.60 +0.6 814,188 + GFF825 0.60 -1.6 814,193 + GFF825 0.61 -1.3 814,193 + GFF825 0.61 -1.7 814,195 - GFF825 0.61 -1.1 814,196 - GFF825 0.61 -2.1 814,207 + GFF825 0.62 +0.5 814,207 + GFF825 0.62 -2.4 814,211 + GFF825 0.63 -0.3 814,211 + GFF825 0.63 -1.3 814,221 + GFF825 0.64 -1.4 814,242 + GFF825 0.66 -1.6 814,250 - GFF825 0.67 -1.1 814,289 + GFF825 0.71 -0.2 814,325 + GFF825 0.74 -2.2 814,336 + GFF825 0.75 -1.5 814,338 + GFF825 0.76 -1.4 814,346 - GFF825 0.76 -1.7 814,348 + GFF825 0.77 -1.5 814,356 - GFF825 0.77 -1.2 814,384 + GFF825 0.80 -1.1 814,384 + GFF825 0.80 -1.2 814,392 - GFF825 0.81 -1.1 814,467 + GFF825 0.89 -2.3 814,469 - GFF825 0.89 -1.3 814,470 - GFF825 0.89 -1.5 814,482 - -2.1 814,501 - -1.2 814,503 + -1.2 814,511 - -1.1 814,573 + +0.5 814,573 + -1.8 814,579 - -1.1 814,595 - +0.9
Or see this region's nucleotide sequence