Experiment: EMM_noCarbon with Sucrose and Putrescine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF598 and GFF599 are separated by 170 nucleotides GFF599 and GFF600 are separated by 32 nucleotides GFF600 and GFF601 overlap by 4 nucleotides GFF601 and GFF602 are separated by 35 nucleotides GFF602 and GFF603 are separated by 41 nucleotides GFF603 and GFF604 are separated by 4 nucleotides
GFF598 - Protein-disulfide isomerase, at 602,260 to 602,970
GFF598
GFF599 - LSU ribosomal protein L34p, at 603,141 to 603,275
GFF599
GFF600 - Ribonuclease P protein component (EC 3.1.26.5), at 603,308 to 603,709
GFF600
GFF601 - Membrane protein insertion efficiency factor YidD, at 603,706 to 603,918
GFF601
GFF602 - Inner membrane protein translocase and chaperone YidC, long form, at 603,954 to 605,660
GFF602
GFF603 - GTP-binding protein EngB, at 605,702 to 606,349
GFF603
GFF604 - Protein PhnA, at 606,354 to 606,656
GFF604
Position (kb)
603
604
605
606 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 602.972 kb on + strand at 602.980 kb on - strand at 602.980 kb on - strand at 603.002 kb on + strand at 603.002 kb on + strand at 603.036 kb on - strand at 603.036 kb on - strand at 603.277 kb on - strand at 603.615 kb on - strand, within GFF600 at 603.617 kb on - strand, within GFF600 at 603.624 kb on + strand, within GFF600 at 603.632 kb on + strand, within GFF600 at 603.632 kb on + strand, within GFF600 at 603.632 kb on + strand, within GFF600 at 603.632 kb on + strand, within GFF600 at 603.637 kb on + strand, within GFF600 at 603.640 kb on - strand, within GFF600 at 603.664 kb on + strand, within GFF600 at 603.691 kb on - strand at 603.723 kb on + strand at 603.740 kb on + strand, within GFF601 at 603.740 kb on + strand, within GFF601 at 603.748 kb on - strand, within GFF601 at 603.748 kb on - strand, within GFF601 at 603.754 kb on - strand, within GFF601 at 603.775 kb on + strand, within GFF601 at 603.777 kb on - strand, within GFF601 at 603.782 kb on + strand, within GFF601 at 603.787 kb on + strand, within GFF601 at 603.787 kb on + strand, within GFF601 at 603.787 kb on + strand, within GFF601 at 603.787 kb on + strand, within GFF601 at 603.787 kb on + strand, within GFF601 at 603.792 kb on + strand, within GFF601 at 603.795 kb on - strand, within GFF601 at 603.795 kb on - strand, within GFF601 at 603.795 kb on - strand, within GFF601 at 603.795 kb on - strand, within GFF601 at 603.795 kb on - strand, within GFF601 at 603.805 kb on - strand, within GFF601 at 603.837 kb on + strand, within GFF601 at 603.837 kb on + strand, within GFF601 at 603.882 kb on + strand, within GFF601 at 603.882 kb on + strand, within GFF601 at 603.898 kb on + strand at 603.914 kb on + strand at 605.621 kb on + strand at 605.626 kb on + strand at 605.629 kb on - strand at 605.633 kb on + strand at 606.333 kb on - strand at 606.333 kb on - strand at 606.333 kb on - strand at 606.333 kb on - strand at 606.339 kb on + strand at 606.340 kb on - strand at 606.340 kb on - strand at 606.343 kb on + strand at 606.343 kb on + strand at 606.343 kb on + strand at 606.344 kb on + strand at 606.351 kb on - strand at 606.351 kb on - strand at 606.351 kb on - strand at 606.358 kb on - strand at 606.366 kb on + strand at 606.366 kb on + strand at 606.366 kb on + strand at 606.378 kb on - strand at 606.398 kb on + strand, within GFF604 at 606.406 kb on - strand, within GFF604 at 606.438 kb on - strand, within GFF604 at 606.441 kb on + strand, within GFF604 at 606.442 kb on + strand, within GFF604 at 606.442 kb on + strand, within GFF604 at 606.450 kb on - strand, within GFF604 at 606.484 kb on - strand, within GFF604 at 606.497 kb on - strand, within GFF604 at 606.499 kb on - strand, within GFF604 at 606.500 kb on + strand, within GFF604 at 606.500 kb on + strand, within GFF604 at 606.515 kb on + strand, within GFF604 at 606.537 kb on - strand, within GFF604 at 606.545 kb on + strand, within GFF604 at 606.552 kb on + strand, within GFF604 at 606.562 kb on - strand, within GFF604 at 606.562 kb on - strand, within GFF604 at 606.562 kb on - strand, within GFF604 at 606.562 kb on - strand, within GFF604 at 606.596 kb on - strand, within GFF604 at 606.618 kb on + strand, within GFF604 at 606.619 kb on + strand, within GFF604 at 606.627 kb on - strand at 606.644 kb on - strand at 606.652 kb on + strand at 606.658 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction EMM_noCarbon with Sucrose and Putrescine remove 602,972 + +0.7 602,980 - -0.1 602,980 - +0.2 603,002 + +0.2 603,002 + +0.8 603,036 - -0.9 603,036 - +0.1 603,277 - +0.8 603,615 - GFF600 0.76 -0.1 603,617 - GFF600 0.77 +0.3 603,624 + GFF600 0.79 +2.0 603,632 + GFF600 0.81 -0.3 603,632 + GFF600 0.81 -1.1 603,632 + GFF600 0.81 -0.6 603,632 + GFF600 0.81 -1.0 603,637 + GFF600 0.82 -0.7 603,640 - GFF600 0.83 +0.1 603,664 + GFF600 0.89 -0.7 603,691 - -1.2 603,723 + -0.3 603,740 + GFF601 0.16 -0.9 603,740 + GFF601 0.16 -0.8 603,748 - GFF601 0.20 -1.0 603,748 - GFF601 0.20 -1.9 603,754 - GFF601 0.23 +0.4 603,775 + GFF601 0.32 -1.2 603,777 - GFF601 0.33 -1.2 603,782 + GFF601 0.36 +0.5 603,787 + GFF601 0.38 -2.3 603,787 + GFF601 0.38 +0.8 603,787 + GFF601 0.38 +1.0 603,787 + GFF601 0.38 -0.5 603,787 + GFF601 0.38 +0.5 603,792 + GFF601 0.40 +0.3 603,795 - GFF601 0.42 +0.3 603,795 - GFF601 0.42 -1.4 603,795 - GFF601 0.42 -2.0 603,795 - GFF601 0.42 +1.3 603,795 - GFF601 0.42 -0.2 603,805 - GFF601 0.46 +0.9 603,837 + GFF601 0.62 +0.3 603,837 + GFF601 0.62 -1.1 603,882 + GFF601 0.83 -2.0 603,882 + GFF601 0.83 -2.6 603,898 + +0.9 603,914 + +0.7 605,621 + -0.9 605,626 + +0.2 605,629 - -1.6 605,633 + -0.4 606,333 - -1.7 606,333 - -0.8 606,333 - +0.4 606,333 - -0.3 606,339 + -0.7 606,340 - -1.8 606,340 - +0.9 606,343 + -2.0 606,343 + -1.7 606,343 + -1.4 606,344 + -0.4 606,351 - +1.7 606,351 - +0.3 606,351 - -0.2 606,358 - -1.2 606,366 + +1.2 606,366 + -1.2 606,366 + -1.4 606,378 - -1.4 606,398 + GFF604 0.15 -0.1 606,406 - GFF604 0.17 -0.6 606,438 - GFF604 0.28 +1.1 606,441 + GFF604 0.29 -2.0 606,442 + GFF604 0.29 +0.7 606,442 + GFF604 0.29 -0.6 606,450 - GFF604 0.32 +0.8 606,484 - GFF604 0.43 -1.4 606,497 - GFF604 0.47 -0.2 606,499 - GFF604 0.48 -0.5 606,500 + GFF604 0.48 +1.6 606,500 + GFF604 0.48 +0.7 606,515 + GFF604 0.53 -1.6 606,537 - GFF604 0.60 -0.3 606,545 + GFF604 0.63 -0.2 606,552 + GFF604 0.65 -0.1 606,562 - GFF604 0.69 -2.4 606,562 - GFF604 0.69 -0.7 606,562 - GFF604 0.69 +0.9 606,562 - GFF604 0.69 +0.9 606,596 - GFF604 0.80 +1.8 606,618 + GFF604 0.87 +0.1 606,619 + GFF604 0.87 +1.0 606,627 - -0.1 606,644 - +1.0 606,652 + -0.1 606,658 - +0.1
Or see this region's nucleotide sequence