Experiment: EMM_noCarbon with Sucrose and Putrescine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF533 and GFF534 are separated by 13 nucleotides GFF534 and GFF535 overlap by 1 nucleotides GFF535 and GFF536 are separated by 14 nucleotides GFF536 and GFF537 are separated by 3 nucleotides
GFF533 - 1,2-dihydroxycyclohexa-3,5-diene-1-carboxylate dehydrogenase (EC 1.3.1.25), at 530,195 to 530,980
GFF533
GFF534 - Succinyl-CoA:3-ketoacid-coenzyme A transferase subunit A (EC 2.8.3.5), at 530,994 to 531,701
GFF534
GFF535 - Succinyl-CoA:3-ketoacid-coenzyme A transferase subunit B (EC 2.8.3.5), at 531,701 to 532,348
GFF535
GFF536 - Ferredoxin, 2Fe-2S, at 532,363 to 532,680
GFF536
GFF537 - Beta-ketoadipate enol-lactone hydrolase (EC 3.1.1.24), at 532,684 to 533,454
GFF537
Position (kb)
531
532
533 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 530.715 kb on + strand, within GFF533 at 530.722 kb on + strand, within GFF533 at 530.758 kb on + strand, within GFF533 at 530.760 kb on + strand, within GFF533 at 530.763 kb on + strand, within GFF533 at 530.766 kb on - strand, within GFF533 at 530.768 kb on + strand, within GFF533 at 530.771 kb on - strand, within GFF533 at 530.771 kb on - strand, within GFF533 at 530.771 kb on - strand, within GFF533 at 530.771 kb on - strand, within GFF533 at 530.771 kb on - strand, within GFF533 at 530.771 kb on - strand, within GFF533 at 530.771 kb on - strand, within GFF533 at 530.793 kb on - strand, within GFF533 at 530.805 kb on + strand, within GFF533 at 530.806 kb on - strand, within GFF533 at 530.818 kb on + strand, within GFF533 at 530.826 kb on - strand, within GFF533 at 530.852 kb on + strand, within GFF533 at 530.893 kb on + strand, within GFF533 at 530.916 kb on + strand at 530.916 kb on + strand at 530.921 kb on + strand at 530.924 kb on - strand at 530.924 kb on - strand at 530.924 kb on - strand at 530.943 kb on + strand at 530.943 kb on + strand at 530.951 kb on - strand at 530.951 kb on - strand at 530.951 kb on - strand at 530.955 kb on - strand at 530.956 kb on - strand at 530.956 kb on - strand at 530.991 kb on - strand at 531.033 kb on + strand at 531.070 kb on + strand, within GFF534 at 531.070 kb on + strand, within GFF534 at 531.070 kb on + strand, within GFF534 at 531.070 kb on + strand, within GFF534 at 531.070 kb on + strand, within GFF534 at 531.078 kb on - strand, within GFF534 at 531.078 kb on - strand, within GFF534 at 531.078 kb on - strand, within GFF534 at 531.078 kb on - strand, within GFF534 at 531.078 kb on - strand, within GFF534 at 531.088 kb on + strand, within GFF534 at 531.091 kb on + strand, within GFF534 at 531.169 kb on + strand, within GFF534 at 531.169 kb on + strand, within GFF534 at 531.220 kb on + strand, within GFF534 at 531.228 kb on - strand, within GFF534 at 531.235 kb on + strand, within GFF534 at 531.243 kb on - strand, within GFF534 at 531.245 kb on + strand, within GFF534 at 531.291 kb on - strand, within GFF534 at 531.345 kb on + strand, within GFF534 at 531.367 kb on + strand, within GFF534 at 531.439 kb on + strand, within GFF534 at 531.439 kb on + strand, within GFF534 at 531.439 kb on + strand, within GFF534 at 531.439 kb on + strand, within GFF534 at 531.444 kb on + strand, within GFF534 at 531.447 kb on - strand, within GFF534 at 531.452 kb on - strand, within GFF534 at 531.463 kb on - strand, within GFF534 at 531.497 kb on + strand, within GFF534 at 531.497 kb on + strand, within GFF534 at 531.497 kb on + strand, within GFF534 at 531.497 kb on + strand, within GFF534 at 531.505 kb on - strand, within GFF534 at 531.505 kb on - strand, within GFF534 at 531.522 kb on - strand, within GFF534 at 531.545 kb on + strand, within GFF534 at 531.545 kb on + strand, within GFF534 at 531.545 kb on + strand, within GFF534 at 531.550 kb on + strand, within GFF534 at 531.553 kb on - strand, within GFF534 at 531.554 kb on + strand, within GFF534 at 531.581 kb on + strand, within GFF534 at 531.584 kb on - strand, within GFF534 at 531.587 kb on + strand, within GFF534 at 531.594 kb on - strand, within GFF534 at 531.594 kb on - strand, within GFF534 at 531.601 kb on - strand, within GFF534 at 531.612 kb on + strand, within GFF534 at 531.636 kb on + strand at 531.644 kb on - strand at 531.647 kb on - strand at 531.777 kb on - strand, within GFF535 at 531.810 kb on - strand, within GFF535 at 531.822 kb on + strand, within GFF535 at 531.824 kb on - strand, within GFF535 at 531.873 kb on - strand, within GFF535 at 531.873 kb on - strand, within GFF535 at 531.887 kb on + strand, within GFF535 at 531.887 kb on + strand, within GFF535 at 531.888 kb on + strand, within GFF535 at 531.888 kb on + strand, within GFF535 at 531.896 kb on - strand, within GFF535 at 531.898 kb on + strand, within GFF535 at 531.901 kb on - strand, within GFF535 at 531.901 kb on - strand, within GFF535 at 531.906 kb on - strand, within GFF535 at 531.932 kb on - strand, within GFF535 at 531.956 kb on + strand, within GFF535 at 531.963 kb on + strand, within GFF535 at 531.964 kb on - strand, within GFF535 at 532.003 kb on + strand, within GFF535 at 532.043 kb on + strand, within GFF535 at 532.066 kb on + strand, within GFF535 at 532.073 kb on + strand, within GFF535 at 532.073 kb on + strand, within GFF535 at 532.080 kb on - strand, within GFF535 at 532.081 kb on - strand, within GFF535 at 532.090 kb on + strand, within GFF535 at 532.096 kb on + strand, within GFF535 at 532.104 kb on - strand, within GFF535 at 532.124 kb on + strand, within GFF535 at 532.162 kb on - strand, within GFF535 at 532.186 kb on - strand, within GFF535 at 532.203 kb on - strand, within GFF535 at 532.218 kb on - strand, within GFF535 at 532.250 kb on - strand, within GFF535 at 532.254 kb on - strand, within GFF535 at 532.256 kb on + strand, within GFF535 at 532.257 kb on + strand, within GFF535 at 532.257 kb on + strand, within GFF535 at 532.265 kb on - strand, within GFF535 at 532.265 kb on - strand, within GFF535 at 532.265 kb on - strand, within GFF535 at 532.265 kb on - strand, within GFF535 at 532.265 kb on - strand, within GFF535 at 532.265 kb on - strand, within GFF535 at 532.265 kb on - strand, within GFF535 at 532.265 kb on - strand, within GFF535 at 532.265 kb on - strand, within GFF535 at 532.279 kb on - strand, within GFF535 at 532.363 kb on - strand at 532.373 kb on - strand at 532.378 kb on + strand at 532.378 kb on + strand at 532.428 kb on + strand, within GFF536 at 532.431 kb on - strand, within GFF536 at 532.442 kb on - strand, within GFF536 at 532.442 kb on - strand, within GFF536 at 532.464 kb on - strand, within GFF536 at 532.465 kb on - strand, within GFF536 at 532.479 kb on + strand, within GFF536 at 532.527 kb on + strand, within GFF536 at 532.527 kb on + strand, within GFF536 at 532.535 kb on - strand, within GFF536 at 532.535 kb on - strand, within GFF536 at 532.573 kb on - strand, within GFF536 at 532.609 kb on + strand, within GFF536 at 532.609 kb on + strand, within GFF536 at 532.617 kb on - strand, within GFF536 at 532.623 kb on + strand, within GFF536 at 532.651 kb on - strand at 532.656 kb on - strand at 532.674 kb on + strand at 532.674 kb on + strand at 532.682 kb on - strand at 532.688 kb on + strand at 532.688 kb on + strand at 532.688 kb on + strand at 532.688 kb on + strand at 532.689 kb on - strand at 532.689 kb on - strand at 532.689 kb on - strand at 532.693 kb on + strand at 532.696 kb on - strand at 532.696 kb on - strand at 532.696 kb on - strand at 532.696 kb on - strand at 532.698 kb on + strand at 532.698 kb on + strand at 532.706 kb on - strand at 532.706 kb on - strand at 532.721 kb on - strand at 532.721 kb on - strand at 532.721 kb on - strand at 532.721 kb on - strand at 532.721 kb on - strand at 532.759 kb on + strand at 532.761 kb on + strand, within GFF537 at 532.761 kb on + strand, within GFF537 at 532.831 kb on + strand, within GFF537 at 532.833 kb on + strand, within GFF537 at 532.834 kb on - strand, within GFF537 at 532.838 kb on + strand, within GFF537 at 532.841 kb on - strand, within GFF537 at 532.891 kb on + strand, within GFF537 at 532.899 kb on - strand, within GFF537 at 532.920 kb on - strand, within GFF537 at 532.936 kb on - strand, within GFF537 at 532.991 kb on + strand, within GFF537 at 532.993 kb on - strand, within GFF537 at 533.034 kb on + strand, within GFF537 at 533.043 kb on - strand, within GFF537 at 533.045 kb on - strand, within GFF537 at 533.050 kb on + strand, within GFF537 at 533.050 kb on + strand, within GFF537 at 533.050 kb on + strand, within GFF537 at 533.050 kb on + strand, within GFF537 at 533.050 kb on - strand, within GFF537 at 533.058 kb on - strand, within GFF537 at 533.082 kb on - strand, within GFF537 at 533.088 kb on + strand, within GFF537 at 533.096 kb on - strand, within GFF537 at 533.100 kb on + strand, within GFF537 at 533.102 kb on - strand, within GFF537 at 533.132 kb on + strand, within GFF537 at 533.168 kb on + strand, within GFF537 at 533.190 kb on + strand, within GFF537 at 533.195 kb on + strand, within GFF537 at 533.195 kb on + strand, within GFF537 at 533.203 kb on + strand, within GFF537 at 533.203 kb on + strand, within GFF537 at 533.203 kb on + strand, within GFF537 at 533.203 kb on + strand, within GFF537 at 533.203 kb on + strand, within GFF537 at 533.203 kb on - strand, within GFF537 at 533.203 kb on - strand, within GFF537 at 533.208 kb on + strand, within GFF537 at 533.208 kb on + strand, within GFF537 at 533.211 kb on - strand, within GFF537 at 533.211 kb on - strand, within GFF537 at 533.211 kb on - strand, within GFF537 at 533.211 kb on - strand, within GFF537 at 533.211 kb on - strand, within GFF537 at 533.211 kb on - strand, within GFF537 at 533.216 kb on - strand, within GFF537 at 533.216 kb on - strand, within GFF537 at 533.216 kb on - strand, within GFF537 at 533.240 kb on + strand, within GFF537 at 533.240 kb on + strand, within GFF537 at 533.240 kb on + strand, within GFF537 at 533.248 kb on - strand, within GFF537 at 533.252 kb on - strand, within GFF537 at 533.276 kb on + strand, within GFF537 at 533.276 kb on + strand, within GFF537 at 533.276 kb on + strand, within GFF537 at 533.276 kb on + strand, within GFF537 at 533.283 kb on - strand, within GFF537 at 533.301 kb on + strand, within GFF537 at 533.310 kb on + strand, within GFF537 at 533.318 kb on + strand, within GFF537 at 533.326 kb on - strand, within GFF537 at 533.326 kb on - strand, within GFF537 at 533.346 kb on - strand, within GFF537
Per-strain Table
Position Strand Gene LocusTag Fraction EMM_noCarbon with Sucrose and Putrescine remove 530,715 + GFF533 0.66 +1.1 530,722 + GFF533 0.67 +1.2 530,758 + GFF533 0.72 +0.0 530,760 + GFF533 0.72 -0.5 530,763 + GFF533 0.72 -0.9 530,766 - GFF533 0.73 -0.6 530,768 + GFF533 0.73 +0.3 530,771 - GFF533 0.73 +0.4 530,771 - GFF533 0.73 -1.6 530,771 - GFF533 0.73 -0.8 530,771 - GFF533 0.73 +1.7 530,771 - GFF533 0.73 +0.2 530,771 - GFF533 0.73 +1.8 530,771 - GFF533 0.73 -0.3 530,793 - GFF533 0.76 +0.2 530,805 + GFF533 0.78 +0.5 530,806 - GFF533 0.78 -0.3 530,818 + GFF533 0.79 -0.3 530,826 - GFF533 0.80 -0.9 530,852 + GFF533 0.84 -1.5 530,893 + GFF533 0.89 +0.8 530,916 + +1.5 530,916 + -0.4 530,921 + +0.2 530,924 - -0.1 530,924 - +0.1 530,924 - +1.0 530,943 + -0.5 530,943 + -1.9 530,951 - -0.7 530,951 - +0.3 530,951 - -0.3 530,955 - -1.5 530,956 - +0.9 530,956 - -1.4 530,991 - +0.5 531,033 + -1.4 531,070 + GFF534 0.11 +1.1 531,070 + GFF534 0.11 +0.3 531,070 + GFF534 0.11 -0.0 531,070 + GFF534 0.11 +1.0 531,070 + GFF534 0.11 -0.5 531,078 - GFF534 0.12 -0.0 531,078 - GFF534 0.12 +1.4 531,078 - GFF534 0.12 +2.1 531,078 - GFF534 0.12 +0.6 531,078 - GFF534 0.12 -0.7 531,088 + GFF534 0.13 -0.2 531,091 + GFF534 0.14 -3.5 531,169 + GFF534 0.25 +0.5 531,169 + GFF534 0.25 +0.5 531,220 + GFF534 0.32 -1.5 531,228 - GFF534 0.33 -0.3 531,235 + GFF534 0.34 +1.7 531,243 - GFF534 0.35 -0.6 531,245 + GFF534 0.35 -1.5 531,291 - GFF534 0.42 +0.7 531,345 + GFF534 0.50 -2.7 531,367 + GFF534 0.53 +1.2 531,439 + GFF534 0.63 +0.4 531,439 + GFF534 0.63 +0.6 531,439 + GFF534 0.63 +0.9 531,439 + GFF534 0.63 +0.5 531,444 + GFF534 0.64 -1.7 531,447 - GFF534 0.64 +0.3 531,452 - GFF534 0.65 -1.8 531,463 - GFF534 0.66 -0.1 531,497 + GFF534 0.71 -1.3 531,497 + GFF534 0.71 +0.2 531,497 + GFF534 0.71 -0.0 531,497 + GFF534 0.71 -0.0 531,505 - GFF534 0.72 -1.3 531,505 - GFF534 0.72 -0.6 531,522 - GFF534 0.75 -0.3 531,545 + GFF534 0.78 -2.4 531,545 + GFF534 0.78 -0.9 531,545 + GFF534 0.78 -0.7 531,550 + GFF534 0.79 +0.8 531,553 - GFF534 0.79 +0.9 531,554 + GFF534 0.79 +0.5 531,581 + GFF534 0.83 -2.5 531,584 - GFF534 0.83 -1.8 531,587 + GFF534 0.84 +1.2 531,594 - GFF534 0.85 +0.6 531,594 - GFF534 0.85 +0.3 531,601 - GFF534 0.86 +0.2 531,612 + GFF534 0.87 +0.8 531,636 + +0.6 531,644 - -1.0 531,647 - +1.0 531,777 - GFF535 0.12 +0.8 531,810 - GFF535 0.17 -0.0 531,822 + GFF535 0.19 -0.8 531,824 - GFF535 0.19 +0.9 531,873 - GFF535 0.27 -0.7 531,873 - GFF535 0.27 -1.5 531,887 + GFF535 0.29 -2.1 531,887 + GFF535 0.29 -1.5 531,888 + GFF535 0.29 +1.0 531,888 + GFF535 0.29 +0.4 531,896 - GFF535 0.30 -1.4 531,898 + GFF535 0.30 +0.0 531,901 - GFF535 0.31 -0.6 531,901 - GFF535 0.31 -1.9 531,906 - GFF535 0.32 -0.4 531,932 - GFF535 0.36 -0.7 531,956 + GFF535 0.39 -0.4 531,963 + GFF535 0.40 +1.1 531,964 - GFF535 0.41 -0.8 532,003 + GFF535 0.47 +0.4 532,043 + GFF535 0.53 -2.0 532,066 + GFF535 0.56 -0.8 532,073 + GFF535 0.57 +1.1 532,073 + GFF535 0.57 +0.4 532,080 - GFF535 0.58 -0.3 532,081 - GFF535 0.59 +0.0 532,090 + GFF535 0.60 -1.3 532,096 + GFF535 0.61 -0.1 532,104 - GFF535 0.62 +2.5 532,124 + GFF535 0.65 -0.6 532,162 - GFF535 0.71 +0.0 532,186 - GFF535 0.75 -2.0 532,203 - GFF535 0.77 +0.5 532,218 - GFF535 0.80 +1.0 532,250 - GFF535 0.85 -0.0 532,254 - GFF535 0.85 +0.1 532,256 + GFF535 0.86 +0.2 532,257 + GFF535 0.86 +1.6 532,257 + GFF535 0.86 +0.5 532,265 - GFF535 0.87 +0.6 532,265 - GFF535 0.87 +1.1 532,265 - GFF535 0.87 -1.6 532,265 - GFF535 0.87 -1.2 532,265 - GFF535 0.87 -0.6 532,265 - GFF535 0.87 -0.5 532,265 - GFF535 0.87 -0.6 532,265 - GFF535 0.87 +2.1 532,265 - GFF535 0.87 -0.9 532,279 - GFF535 0.89 -1.3 532,363 - -0.2 532,373 - -1.0 532,378 + +0.4 532,378 + +1.5 532,428 + GFF536 0.20 +0.3 532,431 - GFF536 0.21 +1.8 532,442 - GFF536 0.25 +1.2 532,442 - GFF536 0.25 +0.2 532,464 - GFF536 0.32 -1.7 532,465 - GFF536 0.32 -1.6 532,479 + GFF536 0.36 +0.3 532,527 + GFF536 0.52 -1.4 532,527 + GFF536 0.52 -0.3 532,535 - GFF536 0.54 -2.2 532,535 - GFF536 0.54 -0.6 532,573 - GFF536 0.66 -1.2 532,609 + GFF536 0.77 -0.0 532,609 + GFF536 0.77 -0.0 532,617 - GFF536 0.80 -0.2 532,623 + GFF536 0.82 -0.7 532,651 - -0.2 532,656 - -0.8 532,674 + -1.5 532,674 + +1.4 532,682 - +1.8 532,688 + +0.5 532,688 + -0.5 532,688 + -0.8 532,688 + +0.6 532,689 - +0.2 532,689 - +0.1 532,689 - +0.5 532,693 + -0.9 532,696 - -0.8 532,696 - -0.1 532,696 - -2.5 532,696 - -0.2 532,698 + -0.2 532,698 + -0.2 532,706 - +1.0 532,706 - +0.3 532,721 - +0.8 532,721 - -0.0 532,721 - -0.9 532,721 - +0.5 532,721 - -0.4 532,759 + +1.1 532,761 + GFF537 0.10 +1.5 532,761 + GFF537 0.10 -1.5 532,831 + GFF537 0.19 +1.7 532,833 + GFF537 0.19 -0.3 532,834 - GFF537 0.19 -0.6 532,838 + GFF537 0.20 +0.3 532,841 - GFF537 0.20 -0.7 532,891 + GFF537 0.27 +1.0 532,899 - GFF537 0.28 -0.6 532,920 - GFF537 0.31 +1.0 532,936 - GFF537 0.33 +0.6 532,991 + GFF537 0.40 +1.8 532,993 - GFF537 0.40 -1.1 533,034 + GFF537 0.45 +0.0 533,043 - GFF537 0.47 -0.0 533,045 - GFF537 0.47 -1.5 533,050 + GFF537 0.47 -0.5 533,050 + GFF537 0.47 +0.2 533,050 + GFF537 0.47 -1.8 533,050 + GFF537 0.47 +0.1 533,050 - GFF537 0.47 +0.1 533,058 - GFF537 0.49 +0.7 533,082 - GFF537 0.52 -0.7 533,088 + GFF537 0.52 +0.2 533,096 - GFF537 0.53 +0.2 533,100 + GFF537 0.54 -0.4 533,102 - GFF537 0.54 +1.1 533,132 + GFF537 0.58 +0.5 533,168 + GFF537 0.63 -0.5 533,190 + GFF537 0.66 +2.1 533,195 + GFF537 0.66 -1.0 533,195 + GFF537 0.66 -0.0 533,203 + GFF537 0.67 +0.9 533,203 + GFF537 0.67 -1.0 533,203 + GFF537 0.67 +0.0 533,203 + GFF537 0.67 +1.2 533,203 + GFF537 0.67 +0.2 533,203 - GFF537 0.67 -0.7 533,203 - GFF537 0.67 +2.9 533,208 + GFF537 0.68 -0.8 533,208 + GFF537 0.68 -0.2 533,211 - GFF537 0.68 -0.0 533,211 - GFF537 0.68 +0.2 533,211 - GFF537 0.68 +0.5 533,211 - GFF537 0.68 +0.7 533,211 - GFF537 0.68 +1.1 533,211 - GFF537 0.68 -0.4 533,216 - GFF537 0.69 -0.3 533,216 - GFF537 0.69 -1.2 533,216 - GFF537 0.69 +0.1 533,240 + GFF537 0.72 +0.1 533,240 + GFF537 0.72 +0.1 533,240 + GFF537 0.72 -0.5 533,248 - GFF537 0.73 +0.2 533,252 - GFF537 0.74 -1.0 533,276 + GFF537 0.77 +0.3 533,276 + GFF537 0.77 +0.4 533,276 + GFF537 0.77 +1.1 533,276 + GFF537 0.77 -0.5 533,283 - GFF537 0.78 +1.2 533,301 + GFF537 0.80 +1.2 533,310 + GFF537 0.81 -0.7 533,318 + GFF537 0.82 -0.8 533,326 - GFF537 0.83 -1.3 533,326 - GFF537 0.83 -1.0 533,346 - GFF537 0.86 +0.4
Or see this region's nucleotide sequence