Experiment: EMM_noCarbon with Sucrose and Putrescine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF386 and GFF387 are separated by 180 nucleotides GFF387 and GFF388 are separated by 7 nucleotides GFF388 and GFF389 are separated by 49 nucleotides
GFF386 - Formamidopyrimidine-DNA glycosylase (EC 3.2.2.23), at 377,341 to 378,153
GFF386
GFF387 - 2-methoxy-6-polyprenyl-1,4-benzoquinol methylase (EC 2.1.1.201), at 378,334 to 379,065
GFF387
GFF388 - Ubiquinone biosynthesis regulatory protein kinase UbiB, at 379,073 to 380,614
GFF388
GFF389 - Phosphopantothenoylcysteine decarboxylase (EC 4.1.1.36) / Phosphopantothenoylcysteine synthetase (EC 6.3.2.5), at 380,664 to 381,908
GFF389
Position (kb) 379
380
381 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 378.077 kb on + strand at 378.079 kb on - strand at 378.079 kb on - strand at 378.079 kb on - strand at 378.079 kb on - strand at 378.079 kb on - strand at 378.079 kb on - strand at 378.080 kb on + strand at 378.081 kb on + strand at 378.081 kb on + strand at 378.081 kb on + strand at 378.085 kb on - strand at 378.087 kb on + strand at 378.087 kb on + strand at 378.097 kb on + strand at 378.097 kb on + strand at 378.097 kb on + strand at 378.097 kb on + strand at 378.097 kb on + strand at 378.100 kb on - strand at 378.105 kb on - strand at 378.105 kb on - strand at 378.107 kb on + strand at 378.122 kb on - strand at 378.122 kb on - strand at 378.144 kb on + strand at 378.144 kb on + strand at 378.144 kb on + strand at 378.144 kb on + strand at 378.149 kb on + strand at 378.149 kb on + strand at 378.149 kb on + strand at 378.149 kb on + strand at 378.149 kb on + strand at 378.152 kb on - strand at 378.152 kb on - strand at 378.152 kb on - strand at 378.152 kb on - strand at 378.152 kb on - strand at 378.152 kb on - strand at 378.157 kb on - strand at 378.157 kb on - strand at 378.157 kb on - strand at 378.205 kb on + strand at 378.205 kb on + strand at 378.205 kb on + strand at 378.205 kb on + strand at 378.206 kb on + strand at 378.212 kb on + strand at 378.213 kb on - strand at 378.213 kb on - strand at 378.213 kb on - strand at 378.213 kb on - strand at 378.214 kb on - strand at 378.214 kb on - strand at 378.220 kb on - strand at 378.229 kb on + strand at 378.236 kb on + strand at 378.244 kb on - strand at 378.252 kb on + strand at 378.263 kb on - strand at 378.265 kb on + strand at 378.268 kb on - strand at 378.273 kb on - strand at 378.295 kb on + strand at 378.297 kb on + strand at 378.304 kb on - strand at 378.320 kb on + strand at 379.059 kb on + strand at 379.059 kb on + strand at 379.078 kb on - strand at 379.088 kb on + strand at 380.451 kb on - strand, within GFF388 at 380.508 kb on + strand at 380.558 kb on + strand at 380.560 kb on + strand at 380.603 kb on + strand at 380.626 kb on + strand at 380.630 kb on + strand at 380.630 kb on + strand at 380.630 kb on + strand at 380.635 kb on + strand at 380.644 kb on - strand at 380.650 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction EMM_noCarbon with Sucrose and Putrescine remove 378,077 + -1.5 378,079 - +0.9 378,079 - -0.7 378,079 - -0.9 378,079 - +0.1 378,079 - +0.7 378,079 - +0.9 378,080 + -1.2 378,081 + -0.1 378,081 + +0.8 378,081 + +0.7 378,085 - -0.2 378,087 + +0.3 378,087 + +0.5 378,097 + -0.9 378,097 + -1.7 378,097 + -0.8 378,097 + -2.4 378,097 + -2.8 378,100 - -0.7 378,105 - -0.4 378,105 - -0.5 378,107 + -1.5 378,122 - -0.6 378,122 - +0.3 378,144 + -0.8 378,144 + +0.6 378,144 + +1.6 378,144 + -0.7 378,149 + -0.4 378,149 + -0.1 378,149 + -1.3 378,149 + -1.9 378,149 + +0.7 378,152 - +0.5 378,152 - -0.2 378,152 - +0.4 378,152 - +1.0 378,152 - -1.7 378,152 - -0.1 378,157 - -0.3 378,157 - -0.5 378,157 - +0.7 378,205 + +0.2 378,205 + -1.4 378,205 + -0.9 378,205 + +0.6 378,206 + +1.8 378,212 + -0.1 378,213 - -2.4 378,213 - -1.4 378,213 - +0.2 378,213 - +0.5 378,214 - -0.7 378,214 - -0.1 378,220 - -0.5 378,229 + -1.7 378,236 + -2.0 378,244 - -0.1 378,252 + -1.0 378,263 - +0.1 378,265 + +0.1 378,268 - -0.7 378,273 - -0.4 378,295 + +0.1 378,297 + -1.5 378,304 - -0.1 378,320 + -0.5 379,059 + -1.0 379,059 + +0.6 379,078 - -1.1 379,088 + +0.4 380,451 - GFF388 0.89 -1.5 380,508 + -1.1 380,558 + +1.5 380,560 + -0.1 380,603 + -0.6 380,626 + -0.4 380,630 + -1.0 380,630 + -1.0 380,630 + -1.2 380,635 + -1.2 380,644 - -0.1 380,650 + +0.8
Or see this region's nucleotide sequence