Experiment: EMM_noCarbon with Sucrose and Putrescine 
Only strains with sufficient reads to estimate fitness are shown,
                   but the strain fitness values are still rather noisy and may be biased towards zero.
                   Strains near the edge of a gene are not shown as being associated with that
                   gene (they are in grey).
                   Strains in the central 10-90% of a gene are color coded by the insertion's strand.
                   Usually, "+" means that the selectable marker is encoded on the forward strand,
                   i.e., transcribed rightward. 
    
        
         
    
        
         
    
        
         
    
        
         
    
         
     
500 nt GFF35 and GFF36 are separated by 79 nucleotides GFF36 and GFF37 are separated by 127 nucleotides GFF37 and GFF38 are separated by 28 nucleotides GFF38 and GFF39 overlap by 4 nucleotides 
        GFF35 - hypothetical protein, at 28,073 to 28,234 
        GFF35 
         
        
        GFF36 - hypothetical protein, at 28,314 to 28,754 
        GFF36 
         
        
        GFF37 - tRNA-Thr-TGT, at 28,882 to 28,956 
        GFF37 
         
        
        GFF38 - FIG00636030: hypothetical protein, at 28,985 to 29,842 
        GFF38 
         
        
        GFF39 - Replication-associated recombination protein RarA, at 29,839 to 31,152 
        GFF39 
         Position (kb) 28 
29 
30 Strain fitness (log2 ratio) -3 
-2 
-1 
0 
1 
2 at 27.985 kb on - strand at 27.994 kb on + strand at 28.000 kb on + strand at 28.005 kb on + strand at 28.005 kb on + strand at 28.008 kb on - strand at 28.014 kb on + strand at 28.028 kb on - strand at 28.039 kb on + strand at 28.055 kb on - strand at 28.056 kb on - strand at 28.063 kb on - strand at 28.063 kb on - strand at 28.063 kb on - strand at 28.063 kb on - strand at 28.086 kb on + strand at 28.087 kb on + strand at 28.094 kb on - strand, within GFF35 at 28.144 kb on - strand, within GFF35 at 28.164 kb on + strand, within GFF35 at 28.172 kb on - strand, within GFF35 at 28.172 kb on - strand, within GFF35 at 28.172 kb on - strand, within GFF35 at 28.172 kb on - strand, within GFF35 at 28.172 kb on - strand, within GFF35 at 28.215 kb on + strand, within GFF35 at 28.215 kb on + strand, within GFF35 at 28.223 kb on - strand at 28.311 kb on + strand at 28.311 kb on + strand at 28.369 kb on + strand, within GFF36 at 28.373 kb on + strand, within GFF36 at 28.387 kb on - strand, within GFF36 at 28.387 kb on - strand, within GFF36 at 28.425 kb on - strand, within GFF36 at 28.433 kb on + strand, within GFF36 at 28.433 kb on + strand, within GFF36 at 28.436 kb on - strand, within GFF36 at 28.441 kb on - strand, within GFF36 at 28.441 kb on - strand, within GFF36 at 28.441 kb on - strand, within GFF36 at 28.441 kb on - strand, within GFF36 at 28.441 kb on - strand, within GFF36 at 28.443 kb on + strand, within GFF36 at 28.443 kb on + strand, within GFF36 at 28.443 kb on + strand, within GFF36 at 28.445 kb on + strand, within GFF36 at 28.451 kb on - strand, within GFF36 at 28.451 kb on - strand, within GFF36 at 28.451 kb on - strand, within GFF36 at 28.457 kb on + strand, within GFF36 at 28.467 kb on + strand, within GFF36 at 28.475 kb on - strand, within GFF36 at 28.475 kb on - strand, within GFF36 at 28.498 kb on - strand, within GFF36 at 28.498 kb on - strand, within GFF36 at 28.498 kb on - strand, within GFF36 at 28.498 kb on - strand, within GFF36 at 28.514 kb on - strand, within GFF36 at 28.514 kb on - strand, within GFF36 at 28.521 kb on + strand, within GFF36 at 28.616 kb on + strand, within GFF36 at 28.616 kb on + strand, within GFF36 at 28.616 kb on + strand, within GFF36 at 28.624 kb on - strand, within GFF36 at 28.624 kb on - strand, within GFF36 at 28.624 kb on - strand, within GFF36 at 28.624 kb on - strand, within GFF36 at 28.624 kb on - strand, within GFF36 at 28.627 kb on - strand, within GFF36 at 28.658 kb on + strand, within GFF36 at 28.659 kb on + strand, within GFF36 at 28.660 kb on - strand, within GFF36 at 28.666 kb on - strand, within GFF36 at 28.666 kb on - strand, within GFF36 at 28.666 kb on - strand, within GFF36 at 28.666 kb on - strand, within GFF36 at 28.666 kb on - strand, within GFF36 at 28.667 kb on - strand, within GFF36 at 28.725 kb on + strand at 28.733 kb on - strand at 28.772 kb on - strand at 29.001 kb on + strand at 29.001 kb on + strand at 29.001 kb on + strand at 29.001 kb on + strand at 29.001 kb on + strand at 29.009 kb on - strand at 29.009 kb on - strand at 29.036 kb on + strand at 29.036 kb on + strand at 29.044 kb on - strand at 29.093 kb on + strand, within GFF38 at 29.101 kb on - strand, within GFF38 at 29.105 kb on + strand, within GFF38 at 29.145 kb on + strand, within GFF38 at 29.151 kb on + strand, within GFF38 at 29.171 kb on - strand, within GFF38 at 29.212 kb on + strand, within GFF38 at 29.229 kb on + strand, within GFF38 at 29.286 kb on + strand, within GFF38 at 29.286 kb on + strand, within GFF38 at 29.291 kb on + strand, within GFF38 at 29.291 kb on + strand, within GFF38 at 29.350 kb on - strand, within GFF38 at 29.390 kb on - strand, within GFF38 at 29.417 kb on - strand, within GFF38 at 29.457 kb on + strand, within GFF38 at 29.457 kb on + strand, within GFF38 at 29.463 kb on + strand, within GFF38 at 29.465 kb on - strand, within GFF38 at 29.465 kb on - strand, within GFF38 at 29.494 kb on - strand, within GFF38 at 29.496 kb on + strand, within GFF38 at 29.496 kb on - strand, within GFF38 at 29.504 kb on - strand, within GFF38 at 29.506 kb on + strand, within GFF38 at 29.514 kb on - strand, within GFF38 at 29.521 kb on + strand, within GFF38 at 29.577 kb on + strand, within GFF38 at 29.585 kb on - strand, within GFF38 at 29.596 kb on + strand, within GFF38 at 29.596 kb on + strand, within GFF38 at 29.596 kb on + strand, within GFF38 at 29.596 kb on + strand, within GFF38 at 29.618 kb on + strand, within GFF38 at 29.618 kb on + strand, within GFF38 at 29.618 kb on + strand, within GFF38 at 29.618 kb on + strand, within GFF38 at 29.626 kb on - strand, within GFF38 at 29.626 kb on - strand, within GFF38 at 29.630 kb on + strand, within GFF38 at 29.635 kb on + strand, within GFF38 at 29.642 kb on + strand, within GFF38 at 29.642 kb on + strand, within GFF38 at 29.653 kb on - strand, within GFF38 at 29.658 kb on - strand, within GFF38 at 29.659 kb on - strand, within GFF38 at 29.670 kb on + strand, within GFF38 at 29.676 kb on - strand, within GFF38 at 29.676 kb on - strand, within GFF38 at 29.678 kb on - strand, within GFF38 at 29.721 kb on + strand, within GFF38 at 29.731 kb on + strand, within GFF38 at 29.744 kb on - strand, within GFF38 at 29.750 kb on - strand, within GFF38 at 29.767 kb on - strand at 29.769 kb on + strand at 29.775 kb on + strand at 29.777 kb on - strand at 29.783 kb on - strand at 29.783 kb on - strand at 29.834 kb on + strand at 29.950 kb on - strand at 29.952 kb on - strand at 30.017 kb on + strand, within GFF39 at 30.035 kb on + strand, within GFF39 at 30.058 kb on + strand, within GFF39 at 30.095 kb on + strand, within GFF39 at 30.103 kb on - strand, within GFF39 at 30.103 kb on - strand, within GFF39 at 30.103 kb on - strand, within GFF39 at 30.109 kb on + strand, within GFF39 at 30.111 kb on + strand, within GFF39 at 30.117 kb on + strand, within GFF39 at 30.163 kb on + strand, within GFF39 at 30.163 kb on + strand, within GFF39 at 30.171 kb on - strand, within GFF39 at 30.193 kb on - strand, within GFF39 at 30.232 kb on + strand, within GFF39 at 30.234 kb on + strand, within GFF39 at 30.237 kb on - strand, within GFF39 at 30.240 kb on - strand, within GFF39 at 30.300 kb on - strand, within GFF39 at 30.346 kb on - strand, within GFF39 at 30.346 kb on - strand, within GFF39 at 30.346 kb on - strand, within GFF39 at 30.346 kb on - strand, within GFF39 at 30.360 kb on + strand, within GFF39 at 30.360 kb on + strand, within GFF39 at 30.403 kb on + strand, within GFF39 at 30.407 kb on - strand, within GFF39 at 30.411 kb on - strand, within GFF39 at 30.430 kb on - strand, within GFF39 at 30.445 kb on + strand, within GFF39 at 30.453 kb on + strand, within GFF39 at 30.467 kb on + strand, within GFF39 at 30.506 kb on + strand, within GFF39 at 30.514 kb on - strand, within GFF39 at 30.545 kb on + strand, within GFF39 at 30.545 kb on + strand, within GFF39 at 30.550 kb on + strand, within GFF39 at 30.555 kb on + strand, within GFF39 at 30.558 kb on - strand, within GFF39 at 30.558 kb on - strand, within GFF39 at 30.558 kb on - strand, within GFF39 at 30.560 kb on + strand, within GFF39 at 30.563 kb on - strand, within GFF39 at 30.568 kb on - strand, within GFF39 at 30.606 kb on - strand, within GFF39 at 30.624 kb on - strand, within GFF39 at 30.641 kb on + strand, within GFF39 at 30.674 kb on + strand, within GFF39 at 30.676 kb on + strand, within GFF39 at 30.676 kb on + strand, within GFF39 at 30.676 kb on + strand, within GFF39 at 30.676 kb on + strand, within GFF39 at 30.676 kb on + strand, within GFF39 at 30.684 kb on - strand, within GFF39 at 30.732 kb on + strand, within GFF39 at 30.740 kb on + strand, within GFF39 at 30.740 kb on - strand, within GFF39 at 30.740 kb on - strand, within GFF39 at 30.755 kb on - strand, within GFF39 at 30.767 kb on + strand, within GFF39 at 30.772 kb on - strand, within GFF39 at 30.792 kb on - strand, within GFF39 at 30.811 kb on + strand, within GFF39 at 30.842 kb on - strand, within GFF39  
 
 
Per-strain Table 
Position  Strand  Gene  LocusTag  Fraction EMM_noCarbon with Sucrose and Putrescine remove 27,985 -  -0.6 27,994 +  -0.4 28,000 +  -0.4 28,005 +  -0.8 28,005 +  +0.0 28,008 -  +0.3 28,014 +  -0.5 28,028 -  +0.5 28,039 +  -2.2 28,055 -  +0.7 28,056 -  +0.3 28,063 -  +0.0 28,063 -  +0.3 28,063 -  -0.9 28,063 -  +0.7 28,086 +  -0.2 28,087 +  -1.4 28,094 -  GFF35 0.13  -0.1 28,144 -  GFF35 0.44  +0.8 28,164 +  GFF35 0.56  -0.8 28,172 -  GFF35 0.61  -2.8 28,172 -  GFF35 0.61  -0.1 28,172 -  GFF35 0.61  -0.3 28,172 -  GFF35 0.61  +0.6 28,172 -  GFF35 0.61  -0.6 28,215 +  GFF35 0.88  -0.3 28,215 +  GFF35 0.88  -1.2 28,223 -  -0.2 28,311 +  +0.3 28,311 +  +0.1 28,369 +  GFF36 0.12  -1.9 28,373 +  GFF36 0.13  -0.2 28,387 -  GFF36 0.17  -0.0 28,387 -  GFF36 0.17  -1.9 28,425 -  GFF36 0.25  +1.8 28,433 +  GFF36 0.27  -0.7 28,433 +  GFF36 0.27  -1.3 28,436 -  GFF36 0.28  -0.0 28,441 -  GFF36 0.29  -1.3 28,441 -  GFF36 0.29  -0.4 28,441 -  GFF36 0.29  -0.5 28,441 -  GFF36 0.29  +0.9 28,441 -  GFF36 0.29  +1.3 28,443 +  GFF36 0.29  +0.0 28,443 +  GFF36 0.29  +0.9 28,443 +  GFF36 0.29  -2.1 28,445 +  GFF36 0.30  +1.3 28,451 -  GFF36 0.31  +0.2 28,451 -  GFF36 0.31  -1.3 28,451 -  GFF36 0.31  -1.5 28,457 +  GFF36 0.32  -1.5 28,467 +  GFF36 0.35  -0.7 28,475 -  GFF36 0.37  +0.0 28,475 -  GFF36 0.37  +1.4 28,498 -  GFF36 0.42  -0.1 28,498 -  GFF36 0.42  +1.5 28,498 -  GFF36 0.42  -0.8 28,498 -  GFF36 0.42  -0.2 28,514 -  GFF36 0.45  +0.4 28,514 -  GFF36 0.45  +1.2 28,521 +  GFF36 0.47  -1.6 28,616 +  GFF36 0.68  -1.0 28,616 +  GFF36 0.68  -0.8 28,616 +  GFF36 0.68  -0.8 28,624 -  GFF36 0.70  +0.3 28,624 -  GFF36 0.70  -0.4 28,624 -  GFF36 0.70  +0.1 28,624 -  GFF36 0.70  +0.4 28,624 -  GFF36 0.70  +0.7 28,627 -  GFF36 0.71  +0.1 28,658 +  GFF36 0.78  +0.5 28,659 +  GFF36 0.78  -0.1 28,660 -  GFF36 0.78  -0.8 28,666 -  GFF36 0.80  +1.4 28,666 -  GFF36 0.80  +1.0 28,666 -  GFF36 0.80  -2.3 28,666 -  GFF36 0.80  -0.1 28,666 -  GFF36 0.80  +0.0 28,667 -  GFF36 0.80  +0.3 28,725 +  +0.2 28,733 -  +0.8 28,772 -  +0.4 29,001 +  +0.9 29,001 +  -0.6 29,001 +  +0.1 29,001 +  +0.7 29,001 +  +0.2 29,009 -  -1.0 29,009 -  -1.1 29,036 +  +0.4 29,036 +  -2.0 29,044 -  +0.2 29,093 +  GFF38 0.13  +0.2 29,101 -  GFF38 0.14  -0.1 29,105 +  GFF38 0.14  -0.7 29,145 +  GFF38 0.19  +0.1 29,151 +  GFF38 0.19  -0.2 29,171 -  GFF38 0.22  +0.6 29,212 +  GFF38 0.26  -1.2 29,229 +  GFF38 0.28  +0.3 29,286 +  GFF38 0.35  +0.9 29,286 +  GFF38 0.35  +0.3 29,291 +  GFF38 0.36  +1.7 29,291 +  GFF38 0.36  -0.6 29,350 -  GFF38 0.43  +0.1 29,390 -  GFF38 0.47  +0.6 29,417 -  GFF38 0.50  -1.3 29,457 +  GFF38 0.55  -1.1 29,457 +  GFF38 0.55  +0.3 29,463 +  GFF38 0.56  -0.3 29,465 -  GFF38 0.56  -0.4 29,465 -  GFF38 0.56  -0.2 29,494 -  GFF38 0.59  +0.2 29,496 +  GFF38 0.60  +0.3 29,496 -  GFF38 0.60  -1.7 29,504 -  GFF38 0.60  +0.9 29,506 +  GFF38 0.61  -1.6 29,514 -  GFF38 0.62  +0.5 29,521 +  GFF38 0.62  -1.1 29,577 +  GFF38 0.69  -1.8 29,585 -  GFF38 0.70  +1.0 29,596 +  GFF38 0.71  -2.0 29,596 +  GFF38 0.71  -0.3 29,596 +  GFF38 0.71  -0.2 29,596 +  GFF38 0.71  -1.8 29,618 +  GFF38 0.74  +1.7 29,618 +  GFF38 0.74  +0.1 29,618 +  GFF38 0.74  -0.8 29,618 +  GFF38 0.74  +1.3 29,626 -  GFF38 0.75  +1.5 29,626 -  GFF38 0.75  -0.2 29,630 +  GFF38 0.75  -1.4 29,635 +  GFF38 0.76  +0.3 29,642 +  GFF38 0.77  -0.6 29,642 +  GFF38 0.77  -2.1 29,653 -  GFF38 0.78  +1.1 29,658 -  GFF38 0.78  -0.6 29,659 -  GFF38 0.79  -0.4 29,670 +  GFF38 0.80  -0.6 29,676 -  GFF38 0.81  +0.3 29,676 -  GFF38 0.81  -0.5 29,678 -  GFF38 0.81  -0.9 29,721 +  GFF38 0.86  -0.8 29,731 +  GFF38 0.87  -1.7 29,744 -  GFF38 0.88  +0.7 29,750 -  GFF38 0.89  +1.3 29,767 -  +0.3 29,769 +  +0.1 29,775 +  -0.3 29,777 -  +0.3 29,783 -  +2.3 29,783 -  +0.1 29,834 +  +0.2 29,950 -  +0.9 29,952 -  -0.7 30,017 +  GFF39 0.14  +0.1 30,035 +  GFF39 0.15  +1.0 30,058 +  GFF39 0.17  -1.7 30,095 +  GFF39 0.19  -0.2 30,103 -  GFF39 0.20  -0.3 30,103 -  GFF39 0.20  -0.3 30,103 -  GFF39 0.20  +1.4 30,109 +  GFF39 0.21  +0.1 30,111 +  GFF39 0.21  +1.0 30,117 +  GFF39 0.21  +0.8 30,163 +  GFF39 0.25  -0.8 30,163 +  GFF39 0.25  +0.6 30,171 -  GFF39 0.25  +0.1 30,193 -  GFF39 0.27  +0.2 30,232 +  GFF39 0.30  -0.1 30,234 +  GFF39 0.30  -2.3 30,237 -  GFF39 0.30  -2.3 30,240 -  GFF39 0.31  +0.0 30,300 -  GFF39 0.35  +0.8 30,346 -  GFF39 0.39  +0.4 30,346 -  GFF39 0.39  +0.0 30,346 -  GFF39 0.39  -0.2 30,346 -  GFF39 0.39  -0.6 30,360 +  GFF39 0.40  +0.2 30,360 +  GFF39 0.40  +0.6 30,403 +  GFF39 0.43  +0.0 30,407 -  GFF39 0.43  -0.5 30,411 -  GFF39 0.44  +0.7 30,430 -  GFF39 0.45  +0.5 30,445 +  GFF39 0.46  +0.8 30,453 +  GFF39 0.47  +0.5 30,467 +  GFF39 0.48  +0.1 30,506 +  GFF39 0.51  +0.6 30,514 -  GFF39 0.51  +0.6 30,545 +  GFF39 0.54  +0.7 30,545 +  GFF39 0.54  +0.0 30,550 +  GFF39 0.54  +2.0 30,555 +  GFF39 0.54  +0.2 30,558 -  GFF39 0.55  -0.6 30,558 -  GFF39 0.55  -1.1 30,558 -  GFF39 0.55  +1.0 30,560 +  GFF39 0.55  +0.1 30,563 -  GFF39 0.55  +0.7 30,568 -  GFF39 0.55  +1.1 30,606 -  GFF39 0.58  -1.0 30,624 -  GFF39 0.60  -0.0 30,641 +  GFF39 0.61  +0.2 30,674 +  GFF39 0.64  -0.3 30,676 +  GFF39 0.64  +0.8 30,676 +  GFF39 0.64  -1.1 30,676 +  GFF39 0.64  +0.3 30,676 +  GFF39 0.64  +0.2 30,676 +  GFF39 0.64  +2.0 30,684 -  GFF39 0.64  +0.3 30,732 +  GFF39 0.68  +0.7 30,740 +  GFF39 0.69  +0.6 30,740 -  GFF39 0.69  -1.3 30,740 -  GFF39 0.69  +0.8 30,755 -  GFF39 0.70  +0.7 30,767 +  GFF39 0.71  +0.2 30,772 -  GFF39 0.71  +0.7 30,792 -  GFF39 0.73  +1.0 30,811 +  GFF39 0.74  +0.7 30,842 -  GFF39 0.76  +0.2 
Or see this region's nucleotide sequence