Experiment: EMM_noCarbon with Sucrose and Putrescine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3458 and GFF3459 are separated by 120 nucleotides GFF3459 and GFF3460 are separated by 38 nucleotides GFF3460 and GFF3461 are separated by 141 nucleotides
GFF3458 - 3-isopropylmalate dehydrogenase (EC 1.1.1.85), at 3,498,154 to 3,499,197
GFF3458
GFF3459 - DNA recombination and repair protein RecO, at 3,499,318 to 3,500,052
GFF3459
GFF3460 - Integral membrane protein TerC, at 3,500,091 to 3,501,071
GFF3460
GFF3461 - Acetyl-CoA hydrolase (EC 3.1.2.1), at 3,501,213 to 3,502,730
GFF3461
Position (kb)
3500
3501
3502 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3499.095 kb on + strand at 3499.095 kb on + strand at 3499.095 kb on + strand at 3499.095 kb on + strand at 3499.103 kb on - strand at 3499.103 kb on - strand at 3499.103 kb on - strand at 3499.103 kb on - strand at 3499.136 kb on + strand at 3499.144 kb on - strand at 3499.180 kb on - strand at 3499.331 kb on + strand at 3499.392 kb on + strand, within GFF3459 at 3499.392 kb on + strand, within GFF3459 at 3499.392 kb on + strand, within GFF3459 at 3499.400 kb on - strand, within GFF3459 at 3499.409 kb on - strand, within GFF3459 at 3499.441 kb on - strand, within GFF3459 at 3499.458 kb on - strand, within GFF3459 at 3499.465 kb on + strand, within GFF3459 at 3499.484 kb on + strand, within GFF3459 at 3499.502 kb on + strand, within GFF3459 at 3499.502 kb on + strand, within GFF3459 at 3499.502 kb on + strand, within GFF3459 at 3499.508 kb on - strand, within GFF3459 at 3499.517 kb on - strand, within GFF3459 at 3499.522 kb on - strand, within GFF3459 at 3499.566 kb on + strand, within GFF3459 at 3499.594 kb on + strand, within GFF3459 at 3499.594 kb on + strand, within GFF3459 at 3499.594 kb on + strand, within GFF3459 at 3499.599 kb on + strand, within GFF3459 at 3499.599 kb on + strand, within GFF3459 at 3499.599 kb on + strand, within GFF3459 at 3499.602 kb on - strand, within GFF3459 at 3499.602 kb on - strand, within GFF3459 at 3499.602 kb on - strand, within GFF3459 at 3499.602 kb on - strand, within GFF3459 at 3499.607 kb on - strand, within GFF3459 at 3499.620 kb on - strand, within GFF3459 at 3499.653 kb on - strand, within GFF3459 at 3499.653 kb on - strand, within GFF3459 at 3499.658 kb on + strand, within GFF3459 at 3499.658 kb on + strand, within GFF3459 at 3499.664 kb on - strand, within GFF3459 at 3499.666 kb on - strand, within GFF3459 at 3499.739 kb on + strand, within GFF3459 at 3499.766 kb on + strand, within GFF3459 at 3499.822 kb on + strand, within GFF3459 at 3499.823 kb on - strand, within GFF3459 at 3499.827 kb on + strand, within GFF3459 at 3499.831 kb on - strand, within GFF3459 at 3499.840 kb on + strand, within GFF3459 at 3499.903 kb on + strand, within GFF3459 at 3499.918 kb on - strand, within GFF3459 at 3499.948 kb on + strand, within GFF3459 at 3499.956 kb on - strand, within GFF3459 at 3499.965 kb on + strand, within GFF3459 at 3499.967 kb on - strand, within GFF3459 at 3499.967 kb on - strand, within GFF3459 at 3499.970 kb on + strand, within GFF3459 at 3499.978 kb on - strand, within GFF3459 at 3500.026 kb on - strand at 3500.026 kb on - strand at 3500.028 kb on + strand at 3500.048 kb on + strand at 3500.056 kb on - strand at 3500.056 kb on - strand at 3500.062 kb on - strand at 3500.111 kb on + strand at 3500.111 kb on + strand at 3500.119 kb on - strand at 3500.119 kb on - strand at 3500.119 kb on - strand at 3500.124 kb on - strand at 3500.144 kb on - strand at 3500.144 kb on - strand at 3500.146 kb on - strand at 3500.151 kb on - strand at 3500.159 kb on - strand at 3500.161 kb on - strand at 3500.164 kb on - strand at 3500.213 kb on - strand, within GFF3460 at 3500.223 kb on + strand, within GFF3460 at 3500.236 kb on + strand, within GFF3460 at 3500.249 kb on - strand, within GFF3460 at 3500.258 kb on - strand, within GFF3460 at 3500.301 kb on + strand, within GFF3460 at 3500.335 kb on - strand, within GFF3460 at 3500.345 kb on - strand, within GFF3460 at 3500.347 kb on + strand, within GFF3460 at 3500.355 kb on - strand, within GFF3460 at 3500.355 kb on - strand, within GFF3460 at 3500.355 kb on - strand, within GFF3460 at 3500.355 kb on - strand, within GFF3460 at 3500.363 kb on + strand, within GFF3460 at 3500.365 kb on - strand, within GFF3460 at 3500.420 kb on - strand, within GFF3460 at 3500.436 kb on - strand, within GFF3460 at 3500.440 kb on + strand, within GFF3460 at 3500.458 kb on + strand, within GFF3460 at 3500.458 kb on + strand, within GFF3460 at 3500.463 kb on + strand, within GFF3460 at 3500.471 kb on - strand, within GFF3460 at 3500.515 kb on + strand, within GFF3460 at 3500.515 kb on + strand, within GFF3460 at 3500.523 kb on - strand, within GFF3460 at 3500.523 kb on - strand, within GFF3460 at 3500.523 kb on - strand, within GFF3460 at 3500.523 kb on - strand, within GFF3460 at 3500.524 kb on - strand, within GFF3460 at 3500.527 kb on - strand, within GFF3460 at 3500.527 kb on - strand, within GFF3460 at 3500.585 kb on + strand, within GFF3460 at 3500.625 kb on + strand, within GFF3460 at 3500.649 kb on - strand, within GFF3460 at 3500.663 kb on + strand, within GFF3460 at 3500.664 kb on + strand, within GFF3460 at 3500.665 kb on + strand, within GFF3460 at 3500.698 kb on + strand, within GFF3460 at 3500.728 kb on + strand, within GFF3460 at 3500.728 kb on + strand, within GFF3460 at 3500.741 kb on - strand, within GFF3460 at 3500.749 kb on - strand, within GFF3460 at 3500.863 kb on + strand, within GFF3460 at 3500.887 kb on - strand, within GFF3460 at 3500.890 kb on + strand, within GFF3460 at 3500.894 kb on + strand, within GFF3460 at 3500.898 kb on - strand, within GFF3460 at 3500.902 kb on - strand, within GFF3460 at 3500.913 kb on + strand, within GFF3460 at 3500.968 kb on - strand, within GFF3460 at 3500.977 kb on + strand at 3501.075 kb on - strand at 3501.076 kb on + strand at 3501.097 kb on + strand at 3501.105 kb on - strand at 3501.105 kb on - strand at 3501.126 kb on - strand at 3501.181 kb on + strand at 3501.184 kb on - strand at 3501.186 kb on + strand at 3501.186 kb on + strand at 3501.189 kb on - strand at 3501.193 kb on - strand at 3501.293 kb on + strand at 3501.293 kb on + strand at 3501.296 kb on - strand at 3501.301 kb on - strand at 3501.301 kb on - strand at 3501.301 kb on - strand at 3501.306 kb on - strand at 3501.347 kb on + strand at 3501.385 kb on - strand, within GFF3461 at 3501.395 kb on + strand, within GFF3461 at 3501.395 kb on + strand, within GFF3461 at 3501.395 kb on + strand, within GFF3461 at 3501.403 kb on - strand, within GFF3461 at 3501.403 kb on - strand, within GFF3461 at 3501.425 kb on + strand, within GFF3461 at 3501.425 kb on + strand, within GFF3461 at 3501.504 kb on - strand, within GFF3461 at 3501.506 kb on + strand, within GFF3461 at 3501.521 kb on - strand, within GFF3461 at 3501.587 kb on - strand, within GFF3461 at 3501.595 kb on + strand, within GFF3461 at 3501.603 kb on - strand, within GFF3461 at 3501.603 kb on - strand, within GFF3461 at 3501.603 kb on - strand, within GFF3461 at 3501.613 kb on - strand, within GFF3461 at 3501.688 kb on - strand, within GFF3461 at 3501.753 kb on - strand, within GFF3461 at 3501.753 kb on - strand, within GFF3461 at 3501.764 kb on + strand, within GFF3461 at 3501.764 kb on + strand, within GFF3461 at 3501.764 kb on + strand, within GFF3461 at 3501.764 kb on + strand, within GFF3461 at 3501.765 kb on - strand, within GFF3461 at 3501.772 kb on - strand, within GFF3461 at 3501.772 kb on - strand, within GFF3461 at 3501.772 kb on - strand, within GFF3461 at 3501.772 kb on - strand, within GFF3461 at 3501.824 kb on + strand, within GFF3461 at 3501.845 kb on - strand, within GFF3461 at 3501.851 kb on + strand, within GFF3461 at 3501.851 kb on + strand, within GFF3461 at 3501.859 kb on - strand, within GFF3461 at 3501.859 kb on - strand, within GFF3461 at 3501.861 kb on - strand, within GFF3461 at 3501.899 kb on + strand, within GFF3461 at 3501.902 kb on - strand, within GFF3461 at 3501.993 kb on - strand, within GFF3461 at 3502.063 kb on + strand, within GFF3461 at 3502.071 kb on - strand, within GFF3461 at 3502.071 kb on - strand, within GFF3461 at 3502.071 kb on - strand, within GFF3461
Per-strain Table
Position Strand Gene LocusTag Fraction EMM_noCarbon with Sucrose and Putrescine remove 3,499,095 + -1.5 3,499,095 + -0.9 3,499,095 + -3.1 3,499,095 + -2.4 3,499,103 - -1.5 3,499,103 - -2.4 3,499,103 - -0.8 3,499,103 - -2.7 3,499,136 + -1.0 3,499,144 - -1.1 3,499,180 - -0.2 3,499,331 + +0.2 3,499,392 + GFF3459 0.10 +0.7 3,499,392 + GFF3459 0.10 -1.6 3,499,392 + GFF3459 0.10 +0.5 3,499,400 - GFF3459 0.11 -0.5 3,499,409 - GFF3459 0.12 +1.5 3,499,441 - GFF3459 0.17 -0.0 3,499,458 - GFF3459 0.19 +0.2 3,499,465 + GFF3459 0.20 +0.4 3,499,484 + GFF3459 0.23 -0.0 3,499,502 + GFF3459 0.25 +0.8 3,499,502 + GFF3459 0.25 +1.3 3,499,502 + GFF3459 0.25 +1.5 3,499,508 - GFF3459 0.26 +0.1 3,499,517 - GFF3459 0.27 -0.1 3,499,522 - GFF3459 0.28 +1.7 3,499,566 + GFF3459 0.34 -1.2 3,499,594 + GFF3459 0.38 +0.6 3,499,594 + GFF3459 0.38 +0.4 3,499,594 + GFF3459 0.38 +0.2 3,499,599 + GFF3459 0.38 -0.8 3,499,599 + GFF3459 0.38 -0.2 3,499,599 + GFF3459 0.38 -0.4 3,499,602 - GFF3459 0.39 +1.0 3,499,602 - GFF3459 0.39 -0.8 3,499,602 - GFF3459 0.39 +0.3 3,499,602 - GFF3459 0.39 -0.3 3,499,607 - GFF3459 0.39 -1.9 3,499,620 - GFF3459 0.41 -0.2 3,499,653 - GFF3459 0.46 -0.4 3,499,653 - GFF3459 0.46 -2.2 3,499,658 + GFF3459 0.46 -0.6 3,499,658 + GFF3459 0.46 +0.9 3,499,664 - GFF3459 0.47 +1.0 3,499,666 - GFF3459 0.47 -0.9 3,499,739 + GFF3459 0.57 +1.0 3,499,766 + GFF3459 0.61 +0.5 3,499,822 + GFF3459 0.69 +0.0 3,499,823 - GFF3459 0.69 -1.0 3,499,827 + GFF3459 0.69 -1.7 3,499,831 - GFF3459 0.70 -1.5 3,499,840 + GFF3459 0.71 -1.2 3,499,903 + GFF3459 0.80 -0.3 3,499,918 - GFF3459 0.82 +0.1 3,499,948 + GFF3459 0.86 -0.2 3,499,956 - GFF3459 0.87 -0.7 3,499,965 + GFF3459 0.88 -0.9 3,499,967 - GFF3459 0.88 +1.4 3,499,967 - GFF3459 0.88 -0.6 3,499,970 + GFF3459 0.89 -0.0 3,499,978 - GFF3459 0.90 +0.1 3,500,026 - +0.8 3,500,026 - +0.3 3,500,028 + -0.9 3,500,048 + -0.8 3,500,056 - -1.0 3,500,056 - -0.3 3,500,062 - -0.5 3,500,111 + -1.1 3,500,111 + -0.7 3,500,119 - +0.2 3,500,119 - +0.6 3,500,119 - +1.8 3,500,124 - -1.6 3,500,144 - -0.3 3,500,144 - +1.2 3,500,146 - +0.6 3,500,151 - -0.6 3,500,159 - -0.5 3,500,161 - +0.2 3,500,164 - +1.1 3,500,213 - GFF3460 0.12 -1.0 3,500,223 + GFF3460 0.13 -1.8 3,500,236 + GFF3460 0.15 -0.4 3,500,249 - GFF3460 0.16 +0.2 3,500,258 - GFF3460 0.17 +1.6 3,500,301 + GFF3460 0.21 -0.6 3,500,335 - GFF3460 0.25 -0.1 3,500,345 - GFF3460 0.26 -1.0 3,500,347 + GFF3460 0.26 -1.6 3,500,355 - GFF3460 0.27 -0.3 3,500,355 - GFF3460 0.27 -0.3 3,500,355 - GFF3460 0.27 -0.0 3,500,355 - GFF3460 0.27 -0.9 3,500,363 + GFF3460 0.28 -0.9 3,500,365 - GFF3460 0.28 -0.4 3,500,420 - GFF3460 0.34 -0.5 3,500,436 - GFF3460 0.35 -0.3 3,500,440 + GFF3460 0.36 -1.6 3,500,458 + GFF3460 0.37 -1.7 3,500,458 + GFF3460 0.37 -1.0 3,500,463 + GFF3460 0.38 -1.9 3,500,471 - GFF3460 0.39 -1.2 3,500,515 + GFF3460 0.43 -0.2 3,500,515 + GFF3460 0.43 +0.3 3,500,523 - GFF3460 0.44 +0.3 3,500,523 - GFF3460 0.44 -0.6 3,500,523 - GFF3460 0.44 -0.7 3,500,523 - GFF3460 0.44 -0.2 3,500,524 - GFF3460 0.44 +0.5 3,500,527 - GFF3460 0.44 +0.2 3,500,527 - GFF3460 0.44 -0.1 3,500,585 + GFF3460 0.50 -0.3 3,500,625 + GFF3460 0.54 -0.4 3,500,649 - GFF3460 0.57 -0.6 3,500,663 + GFF3460 0.58 +1.2 3,500,664 + GFF3460 0.58 -1.1 3,500,665 + GFF3460 0.59 -0.3 3,500,698 + GFF3460 0.62 -2.0 3,500,728 + GFF3460 0.65 -0.2 3,500,728 + GFF3460 0.65 -1.1 3,500,741 - GFF3460 0.66 -2.2 3,500,749 - GFF3460 0.67 -0.6 3,500,863 + GFF3460 0.79 -1.6 3,500,887 - GFF3460 0.81 -0.8 3,500,890 + GFF3460 0.81 -1.1 3,500,894 + GFF3460 0.82 +0.7 3,500,898 - GFF3460 0.82 -1.2 3,500,902 - GFF3460 0.83 +0.0 3,500,913 + GFF3460 0.84 -1.6 3,500,968 - GFF3460 0.89 +0.9 3,500,977 + +0.3 3,501,075 - +0.9 3,501,076 + -0.5 3,501,097 + +0.2 3,501,105 - -0.5 3,501,105 - +0.4 3,501,126 - +0.4 3,501,181 + -0.2 3,501,184 - +1.0 3,501,186 + -0.2 3,501,186 + +0.1 3,501,189 - -3.2 3,501,193 - -2.3 3,501,293 + -1.4 3,501,293 + +0.2 3,501,296 - +0.2 3,501,301 - -1.8 3,501,301 - +0.3 3,501,301 - -1.7 3,501,306 - +0.9 3,501,347 + +0.7 3,501,385 - GFF3461 0.11 +0.6 3,501,395 + GFF3461 0.12 +0.3 3,501,395 + GFF3461 0.12 +0.1 3,501,395 + GFF3461 0.12 -3.0 3,501,403 - GFF3461 0.13 -0.4 3,501,403 - GFF3461 0.13 +0.4 3,501,425 + GFF3461 0.14 -0.2 3,501,425 + GFF3461 0.14 -1.3 3,501,504 - GFF3461 0.19 -0.4 3,501,506 + GFF3461 0.19 +0.8 3,501,521 - GFF3461 0.20 +0.6 3,501,587 - GFF3461 0.25 +0.5 3,501,595 + GFF3461 0.25 -0.8 3,501,603 - GFF3461 0.26 -0.4 3,501,603 - GFF3461 0.26 +0.5 3,501,603 - GFF3461 0.26 -0.7 3,501,613 - GFF3461 0.26 +0.3 3,501,688 - GFF3461 0.31 +0.1 3,501,753 - GFF3461 0.36 +1.0 3,501,753 - GFF3461 0.36 -1.4 3,501,764 + GFF3461 0.36 -0.9 3,501,764 + GFF3461 0.36 +1.5 3,501,764 + GFF3461 0.36 +0.5 3,501,764 + GFF3461 0.36 -0.4 3,501,765 - GFF3461 0.36 -0.3 3,501,772 - GFF3461 0.37 -0.1 3,501,772 - GFF3461 0.37 -0.9 3,501,772 - GFF3461 0.37 -0.8 3,501,772 - GFF3461 0.37 -0.1 3,501,824 + GFF3461 0.40 -1.5 3,501,845 - GFF3461 0.42 -1.0 3,501,851 + GFF3461 0.42 +0.3 3,501,851 + GFF3461 0.42 -0.1 3,501,859 - GFF3461 0.43 -1.4 3,501,859 - GFF3461 0.43 +1.0 3,501,861 - GFF3461 0.43 -0.7 3,501,899 + GFF3461 0.45 +1.2 3,501,902 - GFF3461 0.45 +1.1 3,501,993 - GFF3461 0.51 +0.5 3,502,063 + GFF3461 0.56 -1.5 3,502,071 - GFF3461 0.57 -0.5 3,502,071 - GFF3461 0.57 -1.0 3,502,071 - GFF3461 0.57 -0.5
Or see this region's nucleotide sequence