Experiment: EMM_noCarbon with Sucrose and Putrescine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF30 and GFF31 are separated by 299 nucleotides GFF31 and GFF32 are separated by 77 nucleotides GFF32 and GFF33 are separated by 98 nucleotides GFF33 and GFF34 are separated by 62 nucleotides GFF34 and GFF35 are separated by 160 nucleotides GFF35 and GFF36 are separated by 79 nucleotides
GFF30 - hypothetical protein, at 26,288 to 26,803
GFF30
GFF31 - Phage lysin (EC 3.2.1.17) # Phage lysozyme or muramidase (EC 3.2.1.17), at 27,103 to 27,312
GFF31
GFF32 - hypothetical protein, at 27,390 to 27,506
GFF32
GFF33 - hypothetical protein, at 27,605 to 27,721
GFF33
GFF34 - hypothetical protein, at 27,784 to 27,912
GFF34
GFF35 - hypothetical protein, at 28,073 to 28,234
GFF35
GFF36 - hypothetical protein, at 28,314 to 28,754
GFF36
Position (kb) 27
28 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 26.625 kb on + strand, within GFF30 at 26.627 kb on - strand, within GFF30 at 26.629 kb on + strand, within GFF30 at 26.637 kb on - strand, within GFF30 at 26.642 kb on + strand, within GFF30 at 26.650 kb on - strand, within GFF30 at 26.650 kb on - strand, within GFF30 at 26.650 kb on - strand, within GFF30 at 26.650 kb on - strand, within GFF30 at 26.655 kb on + strand, within GFF30 at 26.655 kb on + strand, within GFF30 at 26.655 kb on - strand, within GFF30 at 26.675 kb on - strand, within GFF30 at 26.685 kb on - strand, within GFF30 at 26.766 kb on - strand at 26.766 kb on - strand at 26.777 kb on - strand at 26.818 kb on - strand at 26.820 kb on - strand at 26.820 kb on - strand at 26.823 kb on + strand at 26.857 kb on - strand at 26.872 kb on - strand at 26.905 kb on + strand at 26.905 kb on + strand at 26.913 kb on - strand at 26.929 kb on - strand at 26.933 kb on - strand at 26.933 kb on - strand at 26.947 kb on + strand at 26.952 kb on + strand at 26.953 kb on - strand at 26.955 kb on - strand at 26.981 kb on + strand at 26.981 kb on + strand at 26.985 kb on + strand at 26.994 kb on - strand at 26.994 kb on - strand at 26.995 kb on - strand at 27.004 kb on - strand at 27.026 kb on + strand at 27.026 kb on - strand at 27.111 kb on + strand at 27.113 kb on - strand at 27.127 kb on + strand, within GFF31 at 27.127 kb on + strand, within GFF31 at 27.129 kb on + strand, within GFF31 at 27.135 kb on - strand, within GFF31 at 27.135 kb on - strand, within GFF31 at 27.135 kb on - strand, within GFF31 at 27.137 kb on - strand, within GFF31 at 27.199 kb on + strand, within GFF31 at 27.200 kb on - strand, within GFF31 at 27.204 kb on + strand, within GFF31 at 27.205 kb on + strand, within GFF31 at 27.208 kb on - strand, within GFF31 at 27.255 kb on + strand, within GFF31 at 27.256 kb on + strand, within GFF31 at 27.256 kb on + strand, within GFF31 at 27.256 kb on + strand, within GFF31 at 27.263 kb on - strand, within GFF31 at 27.263 kb on - strand, within GFF31 at 27.264 kb on - strand, within GFF31 at 27.278 kb on - strand, within GFF31 at 27.308 kb on - strand at 27.309 kb on - strand at 27.335 kb on - strand at 27.340 kb on - strand at 27.369 kb on - strand at 27.388 kb on + strand at 27.388 kb on + strand at 27.396 kb on - strand at 27.396 kb on - strand at 27.396 kb on - strand at 27.396 kb on - strand at 27.396 kb on - strand at 27.400 kb on + strand at 27.400 kb on + strand at 27.402 kb on + strand, within GFF32 at 27.402 kb on + strand, within GFF32 at 27.407 kb on + strand, within GFF32 at 27.410 kb on - strand, within GFF32 at 27.410 kb on - strand, within GFF32 at 27.410 kb on - strand, within GFF32 at 27.410 kb on - strand, within GFF32 at 27.410 kb on - strand, within GFF32 at 27.439 kb on + strand, within GFF32 at 27.441 kb on + strand, within GFF32 at 27.441 kb on + strand, within GFF32 at 27.441 kb on + strand, within GFF32 at 27.441 kb on + strand, within GFF32 at 27.465 kb on - strand, within GFF32 at 27.473 kb on - strand, within GFF32 at 27.484 kb on + strand, within GFF32 at 27.508 kb on + strand at 27.551 kb on + strand at 27.556 kb on + strand at 27.558 kb on + strand at 27.591 kb on + strand at 27.591 kb on + strand at 27.591 kb on + strand at 27.591 kb on + strand at 27.591 kb on + strand at 27.591 kb on + strand at 27.591 kb on + strand at 27.594 kb on - strand at 27.594 kb on - strand at 27.599 kb on - strand at 27.599 kb on - strand at 27.599 kb on - strand at 27.599 kb on - strand at 27.606 kb on + strand at 27.607 kb on + strand at 27.623 kb on - strand, within GFF33 at 27.623 kb on - strand, within GFF33 at 27.650 kb on - strand, within GFF33 at 27.650 kb on - strand, within GFF33 at 27.650 kb on - strand, within GFF33 at 27.700 kb on - strand, within GFF33 at 27.705 kb on + strand, within GFF33 at 27.705 kb on + strand, within GFF33 at 27.729 kb on + strand at 27.731 kb on - strand at 27.731 kb on - strand at 27.854 kb on + strand, within GFF34 at 27.923 kb on - strand at 27.924 kb on - strand at 27.928 kb on + strand at 27.934 kb on - strand at 27.940 kb on + strand at 27.940 kb on + strand at 27.940 kb on + strand at 27.940 kb on + strand at 27.943 kb on - strand at 27.948 kb on - strand at 27.950 kb on + strand at 27.958 kb on - strand at 27.973 kb on + strand at 27.980 kb on - strand at 27.981 kb on + strand at 27.981 kb on - strand at 27.985 kb on - strand at 27.994 kb on + strand at 28.000 kb on + strand at 28.000 kb on + strand at 28.000 kb on + strand at 28.005 kb on + strand at 28.005 kb on + strand at 28.005 kb on + strand at 28.008 kb on - strand at 28.008 kb on - strand at 28.008 kb on - strand at 28.014 kb on + strand at 28.028 kb on + strand at 28.028 kb on - strand at 28.029 kb on + strand at 28.039 kb on + strand at 28.055 kb on + strand at 28.055 kb on - strand at 28.055 kb on - strand at 28.056 kb on - strand at 28.056 kb on - strand at 28.063 kb on - strand at 28.063 kb on - strand at 28.063 kb on - strand at 28.063 kb on - strand at 28.063 kb on - strand at 28.064 kb on + strand at 28.064 kb on - strand at 28.086 kb on + strand at 28.086 kb on + strand at 28.087 kb on + strand at 28.087 kb on + strand at 28.093 kb on + strand, within GFF35 at 28.094 kb on - strand, within GFF35 at 28.101 kb on - strand, within GFF35 at 28.137 kb on - strand, within GFF35 at 28.144 kb on - strand, within GFF35 at 28.144 kb on - strand, within GFF35 at 28.157 kb on - strand, within GFF35 at 28.159 kb on + strand, within GFF35 at 28.159 kb on + strand, within GFF35 at 28.164 kb on + strand, within GFF35 at 28.164 kb on + strand, within GFF35 at 28.164 kb on + strand, within GFF35 at 28.164 kb on + strand, within GFF35 at 28.164 kb on + strand, within GFF35 at 28.164 kb on + strand, within GFF35 at 28.164 kb on + strand, within GFF35 at 28.164 kb on + strand, within GFF35 at 28.164 kb on + strand, within GFF35 at 28.172 kb on - strand, within GFF35 at 28.172 kb on - strand, within GFF35 at 28.172 kb on - strand, within GFF35 at 28.172 kb on - strand, within GFF35 at 28.172 kb on - strand, within GFF35 at 28.172 kb on - strand, within GFF35 at 28.172 kb on - strand, within GFF35 at 28.172 kb on - strand, within GFF35 at 28.172 kb on - strand, within GFF35 at 28.172 kb on - strand, within GFF35 at 28.172 kb on - strand, within GFF35 at 28.189 kb on + strand, within GFF35 at 28.215 kb on + strand, within GFF35 at 28.215 kb on + strand, within GFF35 at 28.215 kb on + strand, within GFF35 at 28.223 kb on - strand at 28.223 kb on - strand at 28.296 kb on + strand at 28.304 kb on - strand at 28.311 kb on + strand at 28.311 kb on + strand at 28.369 kb on + strand, within GFF36 at 28.372 kb on - strand, within GFF36 at 28.373 kb on + strand, within GFF36 at 28.373 kb on + strand, within GFF36 at 28.381 kb on - strand, within GFF36 at 28.387 kb on - strand, within GFF36 at 28.387 kb on - strand, within GFF36 at 28.392 kb on - strand, within GFF36 at 28.407 kb on - strand, within GFF36 at 28.417 kb on + strand, within GFF36 at 28.425 kb on - strand, within GFF36 at 28.433 kb on + strand, within GFF36 at 28.433 kb on + strand, within GFF36 at 28.436 kb on - strand, within GFF36 at 28.441 kb on - strand, within GFF36 at 28.441 kb on - strand, within GFF36 at 28.441 kb on - strand, within GFF36 at 28.441 kb on - strand, within GFF36 at 28.441 kb on - strand, within GFF36 at 28.441 kb on - strand, within GFF36 at 28.443 kb on + strand, within GFF36 at 28.443 kb on + strand, within GFF36 at 28.443 kb on + strand, within GFF36 at 28.443 kb on + strand, within GFF36 at 28.443 kb on + strand, within GFF36 at 28.443 kb on + strand, within GFF36 at 28.445 kb on + strand, within GFF36 at 28.451 kb on - strand, within GFF36 at 28.451 kb on - strand, within GFF36 at 28.452 kb on + strand, within GFF36 at 28.457 kb on + strand, within GFF36 at 28.457 kb on + strand, within GFF36 at 28.457 kb on + strand, within GFF36 at 28.460 kb on - strand, within GFF36 at 28.467 kb on + strand, within GFF36 at 28.467 kb on + strand, within GFF36 at 28.475 kb on - strand, within GFF36 at 28.475 kb on - strand, within GFF36 at 28.498 kb on - strand, within GFF36 at 28.498 kb on - strand, within GFF36 at 28.498 kb on - strand, within GFF36 at 28.498 kb on - strand, within GFF36 at 28.498 kb on - strand, within GFF36 at 28.498 kb on - strand, within GFF36 at 28.514 kb on - strand, within GFF36 at 28.514 kb on - strand, within GFF36 at 28.521 kb on + strand, within GFF36 at 28.521 kb on + strand, within GFF36 at 28.521 kb on + strand, within GFF36 at 28.521 kb on + strand, within GFF36 at 28.521 kb on + strand, within GFF36 at 28.521 kb on + strand, within GFF36 at 28.521 kb on + strand, within GFF36 at 28.537 kb on + strand, within GFF36 at 28.550 kb on + strand, within GFF36 at 28.569 kb on - strand, within GFF36 at 28.600 kb on - strand, within GFF36 at 28.616 kb on + strand, within GFF36 at 28.616 kb on + strand, within GFF36 at 28.616 kb on + strand, within GFF36 at 28.618 kb on + strand, within GFF36 at 28.624 kb on - strand, within GFF36 at 28.624 kb on - strand, within GFF36 at 28.624 kb on - strand, within GFF36 at 28.624 kb on - strand, within GFF36 at 28.624 kb on - strand, within GFF36 at 28.624 kb on - strand, within GFF36 at 28.624 kb on - strand, within GFF36 at 28.627 kb on - strand, within GFF36 at 28.658 kb on + strand, within GFF36 at 28.658 kb on + strand, within GFF36 at 28.658 kb on + strand, within GFF36 at 28.658 kb on + strand, within GFF36 at 28.660 kb on - strand, within GFF36 at 28.666 kb on - strand, within GFF36 at 28.666 kb on - strand, within GFF36 at 28.666 kb on - strand, within GFF36 at 28.666 kb on - strand, within GFF36 at 28.666 kb on - strand, within GFF36 at 28.667 kb on - strand, within GFF36 at 28.720 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction EMM_noCarbon with Sucrose and Putrescine remove 26,625 + GFF30 0.65 +1.3 26,627 - GFF30 0.66 -0.8 26,629 + GFF30 0.66 -0.8 26,637 - GFF30 0.68 -0.1 26,642 + GFF30 0.69 -1.2 26,650 - GFF30 0.70 -0.1 26,650 - GFF30 0.70 -0.5 26,650 - GFF30 0.70 +1.4 26,650 - GFF30 0.70 +0.5 26,655 + GFF30 0.71 -0.1 26,655 + GFF30 0.71 +0.2 26,655 - GFF30 0.71 -1.1 26,675 - GFF30 0.75 +0.9 26,685 - GFF30 0.77 +1.3 26,766 - +1.5 26,766 - -2.4 26,777 - +0.8 26,818 - -0.9 26,820 - +1.2 26,820 - -0.3 26,823 + -1.8 26,857 - -0.4 26,872 - +0.3 26,905 + -1.6 26,905 + -0.8 26,913 - -0.6 26,929 - +0.6 26,933 - -0.1 26,933 - -1.5 26,947 + -0.2 26,952 + -0.4 26,953 - -0.2 26,955 - -2.0 26,981 + -2.2 26,981 + +0.7 26,985 + -1.5 26,994 - -0.1 26,994 - -1.3 26,995 - +0.7 27,004 - -0.7 27,026 + -0.4 27,026 - -0.5 27,111 + -0.7 27,113 - -0.3 27,127 + GFF31 0.11 -2.1 27,127 + GFF31 0.11 +1.1 27,129 + GFF31 0.12 -0.1 27,135 - GFF31 0.15 -3.0 27,135 - GFF31 0.15 +0.1 27,135 - GFF31 0.15 -0.8 27,137 - GFF31 0.16 -2.2 27,199 + GFF31 0.46 -0.5 27,200 - GFF31 0.46 +0.0 27,204 + GFF31 0.48 -1.1 27,205 + GFF31 0.49 -0.3 27,208 - GFF31 0.50 +0.5 27,255 + GFF31 0.72 -2.3 27,256 + GFF31 0.73 -0.7 27,256 + GFF31 0.73 -0.5 27,256 + GFF31 0.73 -0.1 27,263 - GFF31 0.76 +0.8 27,263 - GFF31 0.76 +0.1 27,264 - GFF31 0.77 -1.5 27,278 - GFF31 0.83 -1.2 27,308 - +0.7 27,309 - +0.2 27,335 - -0.7 27,340 - -0.8 27,369 - -0.2 27,388 + -0.4 27,388 + +0.5 27,396 - -0.3 27,396 - -0.2 27,396 - -0.9 27,396 - -0.7 27,396 - -0.0 27,400 + +0.5 27,400 + +0.0 27,402 + GFF32 0.10 +1.1 27,402 + GFF32 0.10 +0.0 27,407 + GFF32 0.15 -2.1 27,410 - GFF32 0.17 -0.4 27,410 - GFF32 0.17 +0.2 27,410 - GFF32 0.17 -0.2 27,410 - GFF32 0.17 +0.6 27,410 - GFF32 0.17 -1.0 27,439 + GFF32 0.42 -2.5 27,441 + GFF32 0.44 +0.5 27,441 + GFF32 0.44 -0.1 27,441 + GFF32 0.44 -1.2 27,441 + GFF32 0.44 +1.4 27,465 - GFF32 0.64 -1.4 27,473 - GFF32 0.71 +0.0 27,484 + GFF32 0.80 +0.6 27,508 + -0.8 27,551 + +0.5 27,556 + +0.9 27,558 + -0.6 27,591 + +0.1 27,591 + -1.8 27,591 + -0.6 27,591 + -0.4 27,591 + -2.2 27,591 + -0.8 27,591 + -0.2 27,594 - +0.7 27,594 - -1.5 27,599 - +0.0 27,599 - +0.5 27,599 - +0.5 27,599 - +0.9 27,606 + -1.6 27,607 + -1.2 27,623 - GFF33 0.15 -1.9 27,623 - GFF33 0.15 -1.7 27,650 - GFF33 0.38 +0.1 27,650 - GFF33 0.38 -1.6 27,650 - GFF33 0.38 -0.6 27,700 - GFF33 0.81 -1.8 27,705 + GFF33 0.85 -0.9 27,705 + GFF33 0.85 +0.9 27,729 + -1.0 27,731 - -0.7 27,731 - -0.3 27,854 + GFF34 0.54 +0.4 27,923 - +0.5 27,924 - +0.4 27,928 + -3.3 27,934 - -1.8 27,940 + -0.7 27,940 + -1.5 27,940 + -1.4 27,940 + -1.2 27,943 - -2.1 27,948 - -1.3 27,950 + -0.4 27,958 - -0.6 27,973 + -1.6 27,980 - -0.8 27,981 + -0.6 27,981 - -0.5 27,985 - -0.8 27,994 + -0.6 28,000 + -0.6 28,000 + -0.4 28,000 + -1.3 28,005 + -1.0 28,005 + -0.2 28,005 + +0.1 28,008 - +0.1 28,008 - -1.1 28,008 - +0.0 28,014 + -0.7 28,028 + -0.6 28,028 - +0.3 28,029 + -0.6 28,039 + -2.4 28,055 + +0.8 28,055 - -0.5 28,055 - +0.5 28,056 - -0.5 28,056 - +0.1 28,063 - -0.2 28,063 - +0.1 28,063 - +0.1 28,063 - -1.1 28,063 - +0.5 28,064 + +2.1 28,064 - -0.9 28,086 + -0.4 28,086 + +1.2 28,087 + -1.1 28,087 + -1.6 28,093 + GFF35 0.12 -1.6 28,094 - GFF35 0.13 -0.3 28,101 - GFF35 0.17 -0.8 28,137 - GFF35 0.40 +0.0 28,144 - GFF35 0.44 -0.8 28,144 - GFF35 0.44 +0.7 28,157 - GFF35 0.52 +0.0 28,159 + GFF35 0.53 +0.2 28,159 + GFF35 0.53 -0.6 28,164 + GFF35 0.56 -1.1 28,164 + GFF35 0.56 -2.4 28,164 + GFF35 0.56 +0.3 28,164 + GFF35 0.56 +1.1 28,164 + GFF35 0.56 +0.0 28,164 + GFF35 0.56 -0.9 28,164 + GFF35 0.56 +1.9 28,164 + GFF35 0.56 -0.0 28,164 + GFF35 0.56 -0.3 28,172 - GFF35 0.61 -3.0 28,172 - GFF35 0.61 -0.0 28,172 - GFF35 0.61 -0.3 28,172 - GFF35 0.61 +0.6 28,172 - GFF35 0.61 +0.7 28,172 - GFF35 0.61 -0.4 28,172 - GFF35 0.61 -2.0 28,172 - GFF35 0.61 -0.5 28,172 - GFF35 0.61 +0.4 28,172 - GFF35 0.61 -0.8 28,172 - GFF35 0.61 -1.5 28,189 + GFF35 0.72 +1.7 28,215 + GFF35 0.88 -0.5 28,215 + GFF35 0.88 -1.4 28,215 + GFF35 0.88 -1.6 28,223 - +0.3 28,223 - -0.4 28,296 + -0.1 28,304 - -0.5 28,311 + +0.1 28,311 + -0.1 28,369 + GFF36 0.12 -2.0 28,372 - GFF36 0.13 +1.1 28,373 + GFF36 0.13 +0.5 28,373 + GFF36 0.13 -0.4 28,381 - GFF36 0.15 +1.0 28,387 - GFF36 0.17 -0.2 28,387 - GFF36 0.17 -2.1 28,392 - GFF36 0.18 -1.7 28,407 - GFF36 0.21 +0.8 28,417 + GFF36 0.23 -1.2 28,425 - GFF36 0.25 +1.6 28,433 + GFF36 0.27 -0.9 28,433 + GFF36 0.27 -1.5 28,436 - GFF36 0.28 -0.2 28,441 - GFF36 0.29 -1.1 28,441 - GFF36 0.29 -0.6 28,441 - GFF36 0.29 -0.7 28,441 - GFF36 0.29 +2.2 28,441 - GFF36 0.29 +0.7 28,441 - GFF36 0.29 +1.1 28,443 + GFF36 0.29 -0.1 28,443 + GFF36 0.29 -1.4 28,443 + GFF36 0.29 -0.0 28,443 + GFF36 0.29 +0.7 28,443 + GFF36 0.29 -0.6 28,443 + GFF36 0.29 -2.3 28,445 + GFF36 0.30 +1.2 28,451 - GFF36 0.31 -1.0 28,451 - GFF36 0.31 -1.4 28,452 + GFF36 0.31 +0.9 28,457 + GFF36 0.32 +0.6 28,457 + GFF36 0.32 -1.7 28,457 + GFF36 0.32 -1.4 28,460 - GFF36 0.33 +0.2 28,467 + GFF36 0.35 +0.5 28,467 + GFF36 0.35 -0.8 28,475 - GFF36 0.37 -0.2 28,475 - GFF36 0.37 +1.3 28,498 - GFF36 0.42 -0.2 28,498 - GFF36 0.42 -0.1 28,498 - GFF36 0.42 -1.6 28,498 - GFF36 0.42 -1.0 28,498 - GFF36 0.42 -0.3 28,498 - GFF36 0.42 -0.3 28,514 - GFF36 0.45 +0.2 28,514 - GFF36 0.45 +1.0 28,521 + GFF36 0.47 -1.8 28,521 + GFF36 0.47 -1.7 28,521 + GFF36 0.47 +1.5 28,521 + GFF36 0.47 -1.9 28,521 + GFF36 0.47 -0.2 28,521 + GFF36 0.47 -1.9 28,521 + GFF36 0.47 +0.0 28,537 + GFF36 0.51 -0.8 28,550 + GFF36 0.54 -1.0 28,569 - GFF36 0.58 +1.2 28,600 - GFF36 0.65 +1.2 28,616 + GFF36 0.68 -1.2 28,616 + GFF36 0.68 -1.0 28,616 + GFF36 0.68 -0.9 28,618 + GFF36 0.69 +0.2 28,624 - GFF36 0.70 +0.2 28,624 - GFF36 0.70 -0.6 28,624 - GFF36 0.70 -0.1 28,624 - GFF36 0.70 +0.6 28,624 - GFF36 0.70 +0.1 28,624 - GFF36 0.70 +0.2 28,624 - GFF36 0.70 -0.7 28,627 - GFF36 0.71 -0.1 28,658 + GFF36 0.78 +0.3 28,658 + GFF36 0.78 -1.2 28,658 + GFF36 0.78 -1.5 28,658 + GFF36 0.78 +1.1 28,660 - GFF36 0.78 -1.0 28,666 - GFF36 0.80 +1.3 28,666 - GFF36 0.80 +0.8 28,666 - GFF36 0.80 -2.5 28,666 - GFF36 0.80 -0.3 28,666 - GFF36 0.80 -0.2 28,667 - GFF36 0.80 +0.1 28,720 + -1.1
Or see this region's nucleotide sequence