Strain Fitness in Sphingobium sp. HT1-2 around GFF3294

Experiment: EMM_noCarbon with Sucrose and Putrescine

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF3292 and GFF3293 are separated by 32 nucleotidesGFF3293 and GFF3294 overlap by 4 nucleotidesGFF3294 and GFF3295 are separated by 28 nucleotidesGFF3295 and GFF3296 are separated by 45 nucleotides GFF3292 - N(6)-L-threonylcarbamoyladenine synthase (EC 2.3.1.234), at 3,346,105 to 3,347,151 GFF3292 GFF3293 - Porphobilinogen deaminase (EC 2.5.1.61), at 3,347,184 to 3,348,110 GFF3293 GFF3294 - Uroporphyrinogen-III synthase, at 3,348,107 to 3,348,766 GFF3294 GFF3295 - hypothetical protein, at 3,348,795 to 3,349,667 GFF3295 GFF3296 - hypothetical protein, at 3,349,713 to 3,350,528 GFF3296 Position (kb) 3348 3349Strain fitness (log2 ratio) -3 -2 -1 0 1at 3348.073 kb on + strandat 3348.084 kb on + strandat 3348.098 kb on + strandat 3348.105 kb on + strandat 3348.125 kb on - strandat 3348.742 kb on - strandat 3348.742 kb on - strandat 3348.742 kb on - strandat 3348.755 kb on - strandat 3348.851 kb on + strandat 3349.614 kb on - strandat 3349.624 kb on - strandat 3349.682 kb on + strandat 3349.760 kb on + strandat 3349.760 kb on + strandat 3349.760 kb on + strandat 3349.760 kb on + strandat 3349.760 kb on + strandat 3349.760 kb on + strandat 3349.760 kb on + strandat 3349.760 kb on + strandat 3349.760 kb on + strandat 3349.760 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction EMM_noCarbon with Sucrose and Putrescine
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3,348,073 + -0.1
3,348,084 + -2.8
3,348,098 + +1.7
3,348,105 + -1.9
3,348,125 - +1.7
3,348,742 - -0.4
3,348,742 - -1.7
3,348,742 - -0.2
3,348,755 - +0.4
3,348,851 + -0.1
3,349,614 - -1.1
3,349,624 - +0.1
3,349,682 + -0.4
3,349,760 + +0.4
3,349,760 + +0.1
3,349,760 + +0.2
3,349,760 + -0.0
3,349,760 + +1.2
3,349,760 + -1.7
3,349,760 + -0.7
3,349,760 + +0.0
3,349,760 + -1.3
3,349,760 + -2.8

Or see this region's nucleotide sequence