Experiment: EMM_noCarbon with Sucrose and Putrescine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3292 and GFF3293 are separated by 32 nucleotides GFF3293 and GFF3294 overlap by 4 nucleotides GFF3294 and GFF3295 are separated by 28 nucleotides GFF3295 and GFF3296 are separated by 45 nucleotides
GFF3292 - N(6)-L-threonylcarbamoyladenine synthase (EC 2.3.1.234), at 3,346,105 to 3,347,151
GFF3292
GFF3293 - Porphobilinogen deaminase (EC 2.5.1.61), at 3,347,184 to 3,348,110
GFF3293
GFF3294 - Uroporphyrinogen-III synthase, at 3,348,107 to 3,348,766
GFF3294
GFF3295 - hypothetical protein, at 3,348,795 to 3,349,667
GFF3295
GFF3296 - hypothetical protein, at 3,349,713 to 3,350,528
GFF3296
Position (kb)
3348
3349 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 3348.073 kb on + strand at 3348.084 kb on + strand at 3348.098 kb on + strand at 3348.105 kb on + strand at 3348.125 kb on - strand at 3348.742 kb on - strand at 3348.742 kb on - strand at 3348.742 kb on - strand at 3348.755 kb on - strand at 3348.851 kb on + strand at 3349.614 kb on - strand at 3349.624 kb on - strand at 3349.682 kb on + strand at 3349.760 kb on + strand at 3349.760 kb on + strand at 3349.760 kb on + strand at 3349.760 kb on + strand at 3349.760 kb on + strand at 3349.760 kb on + strand at 3349.760 kb on + strand at 3349.760 kb on + strand at 3349.760 kb on + strand at 3349.760 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction EMM_noCarbon with Sucrose and Putrescine remove 3,348,073 + -0.1 3,348,084 + -2.8 3,348,098 + +1.7 3,348,105 + -1.9 3,348,125 - +1.7 3,348,742 - -0.4 3,348,742 - -1.7 3,348,742 - -0.2 3,348,755 - +0.4 3,348,851 + -0.1 3,349,614 - -1.1 3,349,624 - +0.1 3,349,682 + -0.4 3,349,760 + +0.4 3,349,760 + +0.1 3,349,760 + +0.2 3,349,760 + -0.0 3,349,760 + +1.2 3,349,760 + -1.7 3,349,760 + -0.7 3,349,760 + +0.0 3,349,760 + -1.3 3,349,760 + -2.8
Or see this region's nucleotide sequence