Experiment: EMM_noCarbon with Sucrose and Putrescine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3190 and GFF3191 are separated by 347 nucleotides GFF3191 and GFF3192 are separated by 77 nucleotides GFF3192 and GFF3193 are separated by 125 nucleotides
GFF3190 - hypothetical protein, at 3,250,990 to 3,251,553
GFF3190
GFF3191 - SSU ribosomal protein S13p (S18e), at 3,251,901 to 3,252,269
GFF3191
GFF3192 - SSU ribosomal protein S11p (S14e), at 3,252,347 to 3,252,736
GFF3192
GFF3193 - DNA-directed RNA polymerase alpha subunit (EC 2.7.7.6), at 3,252,862 to 3,253,923
GFF3193
Position (kb)
3252
3253 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3251.350 kb on - strand, within GFF3190 at 3251.350 kb on - strand, within GFF3190 at 3251.425 kb on + strand, within GFF3190 at 3251.448 kb on + strand, within GFF3190 at 3251.453 kb on + strand, within GFF3190 at 3251.461 kb on - strand, within GFF3190 at 3251.471 kb on - strand, within GFF3190 at 3251.478 kb on - strand, within GFF3190 at 3251.478 kb on - strand, within GFF3190 at 3251.483 kb on - strand, within GFF3190 at 3251.485 kb on + strand, within GFF3190 at 3251.489 kb on + strand, within GFF3190 at 3251.497 kb on - strand at 3251.497 kb on - strand at 3251.503 kb on + strand at 3251.508 kb on - strand at 3251.513 kb on + strand at 3251.513 kb on + strand at 3251.517 kb on + strand at 3251.518 kb on + strand at 3251.518 kb on + strand at 3251.518 kb on - strand at 3251.582 kb on - strand at 3251.612 kb on - strand at 3251.663 kb on + strand at 3251.663 kb on - strand at 3251.711 kb on - strand at 3252.279 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction EMM_noCarbon with Sucrose and Putrescine remove 3,251,350 - GFF3190 0.64 +0.7 3,251,350 - GFF3190 0.64 -0.0 3,251,425 + GFF3190 0.77 +0.4 3,251,448 + GFF3190 0.81 -2.7 3,251,453 + GFF3190 0.82 +1.0 3,251,461 - GFF3190 0.84 -1.2 3,251,471 - GFF3190 0.85 +0.9 3,251,478 - GFF3190 0.87 +0.8 3,251,478 - GFF3190 0.87 +0.4 3,251,483 - GFF3190 0.87 -0.8 3,251,485 + GFF3190 0.88 +0.3 3,251,489 + GFF3190 0.88 -0.9 3,251,497 - -1.0 3,251,497 - -1.4 3,251,503 + +1.3 3,251,508 - -1.7 3,251,513 + +0.8 3,251,513 + -1.5 3,251,517 + -0.6 3,251,518 + +0.6 3,251,518 + -0.1 3,251,518 - -0.5 3,251,582 - +0.3 3,251,612 - +1.0 3,251,663 + -1.0 3,251,663 - +1.8 3,251,711 - -2.0 3,252,279 + -1.5
Or see this region's nucleotide sequence