Experiment: EMM_noCarbon with Sucrose and Putrescine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2433 and GFF2434 are separated by 14 nucleotides GFF2434 and GFF2435 overlap by 4 nucleotides GFF2435 and GFF2436 are separated by 23 nucleotides GFF2436 and GFF2437 are separated by 45 nucleotides
GFF2433 - hypothetical protein, at 2,451,260 to 2,451,496
GFF2433
GFF2434 - CDP-diacylglycerol--serine O-phosphatidyltransferase (EC 2.7.8.8) @ Archaetidylserine synthase (EC 2.7.8.38), at 2,451,511 to 2,452,278
GFF2434
GFF2435 - Phosphatidylserine decarboxylase (EC 4.1.1.65), at 2,452,275 to 2,453,009
GFF2435
GFF2436 - hypothetical protein, at 2,453,033 to 2,453,167
GFF2436
GFF2437 - Isocitrate dehydrogenase [NADP] (EC 1.1.1.42), at 2,453,213 to 2,454,433
GFF2437
Position (kb)
2452
2453
2454 Strain fitness (log2 ratio)
-2
-1
0
1 at 2451.278 kb on - strand at 2451.311 kb on + strand, within GFF2433 at 2451.323 kb on + strand, within GFF2433 at 2451.331 kb on - strand, within GFF2433 at 2451.331 kb on - strand, within GFF2433 at 2451.344 kb on + strand, within GFF2433 at 2451.352 kb on - strand, within GFF2433 at 2451.370 kb on + strand, within GFF2433 at 2451.410 kb on - strand, within GFF2433 at 2451.416 kb on + strand, within GFF2433 at 2451.424 kb on - strand, within GFF2433 at 2451.447 kb on + strand, within GFF2433 at 2451.457 kb on + strand, within GFF2433 at 2451.470 kb on - strand, within GFF2433 at 2451.470 kb on - strand, within GFF2433 at 2451.472 kb on + strand, within GFF2433 at 2451.478 kb on - strand at 2451.489 kb on - strand at 2451.491 kb on + strand at 2451.498 kb on - strand at 2451.502 kb on + strand at 2451.503 kb on - strand at 2451.534 kb on + strand at 2451.542 kb on - strand at 2451.542 kb on - strand at 2451.542 kb on - strand at 2453.021 kb on - strand at 2453.038 kb on + strand at 2453.041 kb on + strand at 2453.064 kb on + strand, within GFF2436 at 2453.084 kb on + strand, within GFF2436 at 2453.084 kb on + strand, within GFF2436 at 2453.092 kb on - strand, within GFF2436 at 2453.092 kb on - strand, within GFF2436 at 2453.094 kb on - strand, within GFF2436 at 2453.141 kb on + strand, within GFF2436 at 2453.149 kb on - strand, within GFF2436 at 2453.149 kb on - strand, within GFF2436 at 2453.160 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction EMM_noCarbon with Sucrose and Putrescine remove 2,451,278 - +0.6 2,451,311 + GFF2433 0.22 -1.9 2,451,323 + GFF2433 0.27 -2.1 2,451,331 - GFF2433 0.30 +0.5 2,451,331 - GFF2433 0.30 -1.1 2,451,344 + GFF2433 0.35 -0.6 2,451,352 - GFF2433 0.39 +0.6 2,451,370 + GFF2433 0.46 -1.1 2,451,410 - GFF2433 0.63 -0.4 2,451,416 + GFF2433 0.66 +0.1 2,451,424 - GFF2433 0.69 -0.1 2,451,447 + GFF2433 0.79 +1.2 2,451,457 + GFF2433 0.83 -0.8 2,451,470 - GFF2433 0.89 -0.2 2,451,470 - GFF2433 0.89 -0.4 2,451,472 + GFF2433 0.89 -0.5 2,451,478 - -0.1 2,451,489 - +0.2 2,451,491 + -1.9 2,451,498 - +0.8 2,451,502 + -0.8 2,451,503 - +0.8 2,451,534 + +0.6 2,451,542 - +0.4 2,451,542 - -2.5 2,451,542 - -0.8 2,453,021 - -2.0 2,453,038 + -1.0 2,453,041 + -0.1 2,453,064 + GFF2436 0.23 +0.9 2,453,084 + GFF2436 0.38 -0.8 2,453,084 + GFF2436 0.38 -0.5 2,453,092 - GFF2436 0.44 -0.4 2,453,092 - GFF2436 0.44 -0.3 2,453,094 - GFF2436 0.45 +0.2 2,453,141 + GFF2436 0.80 -0.6 2,453,149 - GFF2436 0.86 +0.2 2,453,149 - GFF2436 0.86 +0.2 2,453,160 + +0.1
Or see this region's nucleotide sequence