Experiment: EMM_noCarbon with Sucrose and Putrescine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2057 and GFF2058 are separated by 115 nucleotides GFF2058 and GFF2059 are separated by 4 nucleotides GFF2059 and GFF2060 are separated by 230 nucleotides
GFF2057 - Ketol-acid reductoisomerase (NADP(+)) (EC 1.1.1.86), at 2,094,215 to 2,095,234
GFF2057
GFF2058 - Protein yceI precursor, at 2,095,350 to 2,095,970
GFF2058
GFF2059 - YceI family protein, at 2,095,975 to 2,096,580
GFF2059
GFF2060 - 2-isopropylmalate synthase (EC 2.3.3.13), at 2,096,811 to 2,098,484
GFF2060
Position (kb)
2095
2096
2097 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2094.983 kb on - strand, within GFF2057 at 2095.000 kb on - strand, within GFF2057 at 2095.091 kb on + strand, within GFF2057 at 2095.099 kb on - strand, within GFF2057 at 2095.111 kb on + strand, within GFF2057 at 2095.117 kb on + strand, within GFF2057 at 2095.119 kb on - strand, within GFF2057 at 2095.128 kb on + strand, within GFF2057 at 2095.142 kb on + strand at 2095.143 kb on + strand at 2095.151 kb on - strand at 2095.167 kb on + strand at 2095.167 kb on + strand at 2095.167 kb on + strand at 2095.175 kb on - strand at 2095.175 kb on - strand at 2095.177 kb on - strand at 2095.221 kb on - strand at 2095.236 kb on - strand at 2095.307 kb on + strand at 2095.307 kb on + strand at 2095.307 kb on + strand at 2095.315 kb on - strand at 2095.359 kb on + strand at 2095.359 kb on + strand at 2095.380 kb on - strand at 2095.385 kb on - strand at 2095.399 kb on + strand at 2095.413 kb on + strand, within GFF2058 at 2095.421 kb on - strand, within GFF2058 at 2095.431 kb on + strand, within GFF2058 at 2095.431 kb on + strand, within GFF2058 at 2095.457 kb on + strand, within GFF2058 at 2095.485 kb on - strand, within GFF2058 at 2095.501 kb on - strand, within GFF2058 at 2095.510 kb on + strand, within GFF2058 at 2095.526 kb on - strand, within GFF2058 at 2095.554 kb on - strand, within GFF2058 at 2095.554 kb on - strand, within GFF2058 at 2095.577 kb on + strand, within GFF2058 at 2095.577 kb on + strand, within GFF2058 at 2095.585 kb on - strand, within GFF2058 at 2095.597 kb on + strand, within GFF2058 at 2095.604 kb on - strand, within GFF2058 at 2095.605 kb on + strand, within GFF2058 at 2095.617 kb on - strand, within GFF2058 at 2095.619 kb on + strand, within GFF2058 at 2095.663 kb on - strand, within GFF2058 at 2095.675 kb on - strand, within GFF2058 at 2095.719 kb on - strand, within GFF2058 at 2095.765 kb on - strand, within GFF2058 at 2095.775 kb on - strand, within GFF2058 at 2095.784 kb on - strand, within GFF2058 at 2095.789 kb on - strand, within GFF2058 at 2095.809 kb on - strand, within GFF2058 at 2095.836 kb on - strand, within GFF2058 at 2095.887 kb on - strand, within GFF2058 at 2095.999 kb on + strand at 2096.037 kb on + strand, within GFF2059 at 2096.168 kb on - strand, within GFF2059 at 2096.168 kb on - strand, within GFF2059 at 2096.168 kb on - strand, within GFF2059 at 2096.168 kb on - strand, within GFF2059 at 2096.195 kb on - strand, within GFF2059 at 2096.200 kb on - strand, within GFF2059 at 2096.200 kb on - strand, within GFF2059 at 2096.316 kb on - strand, within GFF2059 at 2096.340 kb on + strand, within GFF2059 at 2096.348 kb on - strand, within GFF2059 at 2096.389 kb on + strand, within GFF2059 at 2096.389 kb on + strand, within GFF2059 at 2096.397 kb on - strand, within GFF2059 at 2096.397 kb on - strand, within GFF2059 at 2096.397 kb on - strand, within GFF2059 at 2096.397 kb on - strand, within GFF2059 at 2096.397 kb on - strand, within GFF2059 at 2096.402 kb on + strand, within GFF2059 at 2096.424 kb on - strand, within GFF2059 at 2096.437 kb on - strand, within GFF2059 at 2096.506 kb on + strand, within GFF2059 at 2096.520 kb on - strand at 2096.535 kb on + strand at 2096.538 kb on - strand at 2096.548 kb on - strand at 2096.601 kb on + strand at 2096.619 kb on - strand at 2096.629 kb on - strand at 2096.644 kb on - strand at 2096.644 kb on - strand at 2096.690 kb on - strand at 2096.690 kb on - strand at 2096.703 kb on - strand at 2096.711 kb on + strand at 2096.718 kb on + strand at 2096.718 kb on + strand at 2096.718 kb on + strand at 2096.718 kb on + strand at 2096.723 kb on + strand at 2096.726 kb on - strand at 2096.726 kb on - strand at 2096.726 kb on - strand at 2096.771 kb on - strand at 2096.819 kb on + strand at 2096.844 kb on + strand at 2096.862 kb on - strand at 2096.983 kb on + strand, within GFF2060 at 2096.983 kb on + strand, within GFF2060 at 2096.996 kb on + strand, within GFF2060 at 2096.996 kb on + strand, within GFF2060 at 2097.001 kb on + strand, within GFF2060 at 2097.004 kb on - strand, within GFF2060 at 2097.004 kb on - strand, within GFF2060 at 2097.015 kb on - strand, within GFF2060 at 2097.082 kb on - strand, within GFF2060 at 2097.093 kb on - strand, within GFF2060 at 2097.106 kb on + strand, within GFF2060 at 2097.122 kb on - strand, within GFF2060 at 2097.122 kb on - strand, within GFF2060 at 2097.122 kb on - strand, within GFF2060 at 2097.122 kb on - strand, within GFF2060 at 2097.122 kb on - strand, within GFF2060 at 2097.169 kb on + strand, within GFF2060 at 2097.169 kb on + strand, within GFF2060 at 2097.169 kb on + strand, within GFF2060 at 2097.177 kb on - strand, within GFF2060 at 2097.177 kb on - strand, within GFF2060 at 2097.186 kb on + strand, within GFF2060 at 2097.218 kb on + strand, within GFF2060 at 2097.226 kb on - strand, within GFF2060 at 2097.282 kb on - strand, within GFF2060 at 2097.308 kb on + strand, within GFF2060 at 2097.373 kb on + strand, within GFF2060 at 2097.373 kb on + strand, within GFF2060 at 2097.382 kb on + strand, within GFF2060 at 2097.405 kb on - strand, within GFF2060 at 2097.433 kb on + strand, within GFF2060 at 2097.440 kb on - strand, within GFF2060 at 2097.441 kb on - strand, within GFF2060 at 2097.465 kb on + strand, within GFF2060 at 2097.473 kb on + strand, within GFF2060 at 2097.473 kb on + strand, within GFF2060 at 2097.473 kb on + strand, within GFF2060 at 2097.473 kb on + strand, within GFF2060 at 2097.473 kb on + strand, within GFF2060 at 2097.481 kb on - strand, within GFF2060 at 2097.481 kb on - strand, within GFF2060 at 2097.486 kb on - strand, within GFF2060 at 2097.547 kb on + strand, within GFF2060 at 2097.547 kb on + strand, within GFF2060 at 2097.555 kb on - strand, within GFF2060 at 2097.555 kb on - strand, within GFF2060 at 2097.555 kb on - strand, within GFF2060 at 2097.557 kb on - strand, within GFF2060 at 2097.577 kb on + strand, within GFF2060 at 2097.577 kb on + strand, within GFF2060
Per-strain Table
Position Strand Gene LocusTag Fraction EMM_noCarbon with Sucrose and Putrescine remove 2,094,983 - GFF2057 0.75 -1.0 2,095,000 - GFF2057 0.77 -2.1 2,095,091 + GFF2057 0.86 -2.2 2,095,099 - GFF2057 0.87 -0.3 2,095,111 + GFF2057 0.88 -1.5 2,095,117 + GFF2057 0.88 -1.2 2,095,119 - GFF2057 0.89 -3.1 2,095,128 + GFF2057 0.90 -1.0 2,095,142 + -2.2 2,095,143 + -2.8 2,095,151 - -1.0 2,095,167 + -2.5 2,095,167 + -2.8 2,095,167 + -0.1 2,095,175 - -1.8 2,095,175 - -3.1 2,095,177 - -0.7 2,095,221 - -2.0 2,095,236 - -0.6 2,095,307 + -1.2 2,095,307 + -1.9 2,095,307 + +0.7 2,095,315 - -0.0 2,095,359 + +0.1 2,095,359 + +1.0 2,095,380 - -0.3 2,095,385 - +1.1 2,095,399 + -1.4 2,095,413 + GFF2058 0.10 -0.2 2,095,421 - GFF2058 0.11 -0.3 2,095,431 + GFF2058 0.13 +1.9 2,095,431 + GFF2058 0.13 -0.0 2,095,457 + GFF2058 0.17 -0.4 2,095,485 - GFF2058 0.22 +1.2 2,095,501 - GFF2058 0.24 -0.1 2,095,510 + GFF2058 0.26 -0.3 2,095,526 - GFF2058 0.28 -1.0 2,095,554 - GFF2058 0.33 +1.4 2,095,554 - GFF2058 0.33 +0.2 2,095,577 + GFF2058 0.37 -0.4 2,095,577 + GFF2058 0.37 +0.8 2,095,585 - GFF2058 0.38 -0.4 2,095,597 + GFF2058 0.40 -0.2 2,095,604 - GFF2058 0.41 +0.4 2,095,605 + GFF2058 0.41 -0.2 2,095,617 - GFF2058 0.43 +0.3 2,095,619 + GFF2058 0.43 +0.3 2,095,663 - GFF2058 0.50 -0.6 2,095,675 - GFF2058 0.52 +2.3 2,095,719 - GFF2058 0.59 -1.4 2,095,765 - GFF2058 0.67 -0.2 2,095,775 - GFF2058 0.68 -1.1 2,095,784 - GFF2058 0.70 -0.6 2,095,789 - GFF2058 0.71 +1.5 2,095,809 - GFF2058 0.74 +0.9 2,095,836 - GFF2058 0.78 +0.2 2,095,887 - GFF2058 0.86 -1.1 2,095,999 + +0.2 2,096,037 + GFF2059 0.10 -1.2 2,096,168 - GFF2059 0.32 -0.6 2,096,168 - GFF2059 0.32 -1.4 2,096,168 - GFF2059 0.32 +0.0 2,096,168 - GFF2059 0.32 +1.5 2,096,195 - GFF2059 0.36 -1.2 2,096,200 - GFF2059 0.37 -1.1 2,096,200 - GFF2059 0.37 -0.4 2,096,316 - GFF2059 0.56 +1.2 2,096,340 + GFF2059 0.60 +0.0 2,096,348 - GFF2059 0.62 -0.1 2,096,389 + GFF2059 0.68 +0.4 2,096,389 + GFF2059 0.68 +1.3 2,096,397 - GFF2059 0.70 +0.8 2,096,397 - GFF2059 0.70 -0.8 2,096,397 - GFF2059 0.70 +0.7 2,096,397 - GFF2059 0.70 -1.0 2,096,397 - GFF2059 0.70 -2.2 2,096,402 + GFF2059 0.70 -1.7 2,096,424 - GFF2059 0.74 +0.5 2,096,437 - GFF2059 0.76 -1.4 2,096,506 + GFF2059 0.88 -1.3 2,096,520 - -0.5 2,096,535 + -2.0 2,096,538 - -0.5 2,096,548 - -0.5 2,096,601 + +1.0 2,096,619 - -2.4 2,096,629 - -0.9 2,096,644 - -0.1 2,096,644 - -0.6 2,096,690 - -0.7 2,096,690 - -0.6 2,096,703 - -1.7 2,096,711 + -1.6 2,096,718 + -0.0 2,096,718 + -0.2 2,096,718 + -0.0 2,096,718 + +0.4 2,096,723 + +0.2 2,096,726 - -0.2 2,096,726 - -2.8 2,096,726 - -2.5 2,096,771 - +1.0 2,096,819 + -2.2 2,096,844 + -3.1 2,096,862 - -1.4 2,096,983 + GFF2060 0.10 -2.7 2,096,983 + GFF2060 0.10 -1.5 2,096,996 + GFF2060 0.11 -0.7 2,096,996 + GFF2060 0.11 -2.5 2,097,001 + GFF2060 0.11 -3.1 2,097,004 - GFF2060 0.12 -3.0 2,097,004 - GFF2060 0.12 -2.9 2,097,015 - GFF2060 0.12 -1.6 2,097,082 - GFF2060 0.16 +0.2 2,097,093 - GFF2060 0.17 -2.8 2,097,106 + GFF2060 0.18 -1.7 2,097,122 - GFF2060 0.19 -2.6 2,097,122 - GFF2060 0.19 -0.9 2,097,122 - GFF2060 0.19 -0.0 2,097,122 - GFF2060 0.19 -2.0 2,097,122 - GFF2060 0.19 -1.8 2,097,169 + GFF2060 0.21 -2.0 2,097,169 + GFF2060 0.21 -2.5 2,097,169 + GFF2060 0.21 -0.3 2,097,177 - GFF2060 0.22 -1.7 2,097,177 - GFF2060 0.22 -1.7 2,097,186 + GFF2060 0.22 -3.4 2,097,218 + GFF2060 0.24 -2.6 2,097,226 - GFF2060 0.25 -2.1 2,097,282 - GFF2060 0.28 -2.0 2,097,308 + GFF2060 0.30 +0.2 2,097,373 + GFF2060 0.34 -1.7 2,097,373 + GFF2060 0.34 -2.6 2,097,382 + GFF2060 0.34 -2.7 2,097,405 - GFF2060 0.35 -2.7 2,097,433 + GFF2060 0.37 -0.8 2,097,440 - GFF2060 0.38 +1.2 2,097,441 - GFF2060 0.38 -2.6 2,097,465 + GFF2060 0.39 -0.7 2,097,473 + GFF2060 0.40 -0.9 2,097,473 + GFF2060 0.40 -2.2 2,097,473 + GFF2060 0.40 -2.1 2,097,473 + GFF2060 0.40 -0.8 2,097,473 + GFF2060 0.40 -3.0 2,097,481 - GFF2060 0.40 -2.6 2,097,481 - GFF2060 0.40 -1.4 2,097,486 - GFF2060 0.40 -1.0 2,097,547 + GFF2060 0.44 -1.7 2,097,547 + GFF2060 0.44 -2.3 2,097,555 - GFF2060 0.44 -2.2 2,097,555 - GFF2060 0.44 -2.0 2,097,555 - GFF2060 0.44 -2.9 2,097,557 - GFF2060 0.45 -2.5 2,097,577 + GFF2060 0.46 +0.3 2,097,577 + GFF2060 0.46 -2.0
Or see this region's nucleotide sequence