Experiment: EMM_noCarbon with Sucrose and Putrescine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1969 and GFF1970 are separated by 123 nucleotides GFF1970 and GFF1971 are separated by 137 nucleotides GFF1971 and GFF1972 are separated by 5 nucleotides GFF1972 and GFF1973 overlap by 4 nucleotides
GFF1969 - Arginyl-tRNA synthetase (EC 6.1.1.19), at 1,992,771 to 1,994,504
GFF1969
GFF1970 - hypothetical protein, at 1,994,628 to 1,995,266
GFF1970
GFF1971 - 4-hydroxy-3-methylbut-2-enyl diphosphate reductase (EC 1.17.7.4), at 1,995,404 to 1,996,363
GFF1971
GFF1972 - Homoserine kinase (EC 2.7.1.39), at 1,996,369 to 1,997,346
GFF1972
GFF1973 - Ribonuclease HI (EC 3.1.26.4), at 1,997,343 to 1,997,789
GFF1973
Position (kb)
1995
1996
1997 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1994.633 kb on - strand at 1994.658 kb on - strand at 1994.668 kb on - strand at 1994.674 kb on - strand at 1994.682 kb on - strand at 1994.692 kb on - strand, within GFF1970 at 1994.708 kb on - strand, within GFF1970 at 1994.710 kb on + strand, within GFF1970 at 1994.710 kb on + strand, within GFF1970 at 1994.718 kb on - strand, within GFF1970 at 1994.718 kb on - strand, within GFF1970 at 1994.718 kb on - strand, within GFF1970 at 1994.718 kb on - strand, within GFF1970 at 1994.718 kb on - strand, within GFF1970 at 1994.817 kb on - strand, within GFF1970 at 1994.885 kb on - strand, within GFF1970 at 1994.949 kb on - strand, within GFF1970 at 1994.954 kb on - strand, within GFF1970 at 1994.959 kb on - strand, within GFF1970 at 1994.959 kb on - strand, within GFF1970 at 1995.004 kb on - strand, within GFF1970 at 1995.021 kb on - strand, within GFF1970 at 1995.021 kb on - strand, within GFF1970 at 1995.021 kb on - strand, within GFF1970 at 1995.021 kb on - strand, within GFF1970 at 1995.021 kb on - strand, within GFF1970 at 1995.021 kb on - strand, within GFF1970 at 1995.021 kb on - strand, within GFF1970 at 1995.021 kb on - strand, within GFF1970 at 1995.037 kb on - strand, within GFF1970 at 1995.037 kb on - strand, within GFF1970 at 1995.037 kb on - strand, within GFF1970 at 1995.048 kb on - strand, within GFF1970 at 1995.058 kb on - strand, within GFF1970 at 1995.060 kb on - strand, within GFF1970 at 1995.079 kb on - strand, within GFF1970 at 1995.111 kb on - strand, within GFF1970 at 1995.111 kb on - strand, within GFF1970 at 1995.121 kb on - strand, within GFF1970 at 1995.130 kb on - strand, within GFF1970 at 1995.270 kb on - strand at 1995.291 kb on - strand at 1995.302 kb on - strand at 1995.302 kb on - strand at 1995.302 kb on - strand at 1995.302 kb on - strand at 1995.335 kb on + strand at 1995.348 kb on - strand at 1995.354 kb on + strand at 1995.362 kb on - strand at 1995.367 kb on - strand at 1995.388 kb on + strand at 1995.388 kb on + strand at 1995.388 kb on + strand at 1995.388 kb on + strand at 1995.388 kb on + strand at 1995.388 kb on + strand at 1995.388 kb on + strand at 1995.393 kb on + strand at 1996.337 kb on - strand at 1996.337 kb on - strand at 1996.347 kb on + strand at 1996.347 kb on + strand at 1996.355 kb on - strand at 1996.358 kb on + strand at 1996.366 kb on + strand at 1996.401 kb on + strand at 1996.401 kb on + strand at 1996.409 kb on - strand at 1996.410 kb on - strand at 1996.411 kb on + strand at 1996.429 kb on - strand at 1996.430 kb on - strand at 1996.440 kb on - strand at 1996.455 kb on - strand at 1996.459 kb on + strand at 1996.468 kb on + strand, within GFF1972 at 1996.474 kb on - strand, within GFF1972 at 1996.488 kb on + strand, within GFF1972 at 1996.514 kb on - strand, within GFF1972 at 1996.521 kb on + strand, within GFF1972 at 1996.523 kb on + strand, within GFF1972 at 1996.523 kb on + strand, within GFF1972 at 1996.523 kb on + strand, within GFF1972 at 1996.523 kb on + strand, within GFF1972 at 1996.523 kb on + strand, within GFF1972 at 1996.523 kb on + strand, within GFF1972 at 1996.528 kb on + strand, within GFF1972 at 1996.528 kb on + strand, within GFF1972 at 1996.528 kb on + strand, within GFF1972 at 1996.528 kb on + strand, within GFF1972 at 1996.528 kb on + strand, within GFF1972 at 1996.528 kb on + strand, within GFF1972 at 1996.528 kb on + strand, within GFF1972 at 1996.531 kb on - strand, within GFF1972 at 1996.531 kb on - strand, within GFF1972 at 1996.536 kb on - strand, within GFF1972 at 1996.536 kb on - strand, within GFF1972 at 1996.536 kb on - strand, within GFF1972 at 1996.564 kb on + strand, within GFF1972 at 1996.564 kb on + strand, within GFF1972 at 1996.572 kb on - strand, within GFF1972 at 1996.594 kb on - strand, within GFF1972 at 1996.610 kb on + strand, within GFF1972 at 1996.613 kb on + strand, within GFF1972 at 1996.613 kb on + strand, within GFF1972 at 1996.616 kb on - strand, within GFF1972 at 1996.618 kb on + strand, within GFF1972 at 1996.621 kb on - strand, within GFF1972 at 1996.621 kb on - strand, within GFF1972 at 1996.684 kb on - strand, within GFF1972 at 1996.703 kb on + strand, within GFF1972 at 1996.748 kb on + strand, within GFF1972 at 1996.767 kb on + strand, within GFF1972 at 1996.772 kb on + strand, within GFF1972 at 1996.772 kb on + strand, within GFF1972 at 1996.775 kb on - strand, within GFF1972 at 1996.775 kb on - strand, within GFF1972 at 1996.777 kb on + strand, within GFF1972 at 1996.780 kb on - strand, within GFF1972 at 1996.785 kb on - strand, within GFF1972 at 1996.815 kb on - strand, within GFF1972 at 1996.818 kb on + strand, within GFF1972 at 1996.820 kb on - strand, within GFF1972 at 1996.908 kb on - strand, within GFF1972 at 1996.954 kb on - strand, within GFF1972 at 1996.955 kb on + strand, within GFF1972 at 1996.955 kb on + strand, within GFF1972 at 1996.963 kb on - strand, within GFF1972 at 1996.974 kb on - strand, within GFF1972 at 1996.986 kb on - strand, within GFF1972 at 1996.986 kb on - strand, within GFF1972 at 1997.037 kb on + strand, within GFF1972 at 1997.045 kb on + strand, within GFF1972 at 1997.045 kb on + strand, within GFF1972 at 1997.045 kb on + strand, within GFF1972 at 1997.045 kb on + strand, within GFF1972 at 1997.045 kb on + strand, within GFF1972 at 1997.045 kb on + strand, within GFF1972 at 1997.045 kb on - strand, within GFF1972 at 1997.053 kb on - strand, within GFF1972 at 1997.142 kb on - strand, within GFF1972 at 1997.147 kb on + strand, within GFF1972 at 1997.147 kb on + strand, within GFF1972 at 1997.147 kb on + strand, within GFF1972 at 1997.147 kb on + strand, within GFF1972 at 1997.152 kb on + strand, within GFF1972 at 1997.152 kb on + strand, within GFF1972 at 1997.155 kb on - strand, within GFF1972 at 1997.155 kb on - strand, within GFF1972 at 1997.179 kb on + strand, within GFF1972 at 1997.180 kb on + strand, within GFF1972 at 1997.190 kb on + strand, within GFF1972 at 1997.198 kb on + strand, within GFF1972 at 1997.198 kb on - strand, within GFF1972 at 1997.203 kb on + strand, within GFF1972 at 1997.203 kb on + strand, within GFF1972 at 1997.215 kb on + strand, within GFF1972 at 1997.215 kb on + strand, within GFF1972 at 1997.215 kb on + strand, within GFF1972 at 1997.215 kb on + strand, within GFF1972 at 1997.223 kb on - strand, within GFF1972 at 1997.235 kb on - strand, within GFF1972 at 1997.252 kb on + strand at 1997.260 kb on - strand at 1997.278 kb on + strand at 1997.287 kb on + strand at 1997.291 kb on - strand at 1997.292 kb on + strand at 1997.316 kb on + strand at 1997.324 kb on + strand at 1997.329 kb on + strand at 1997.339 kb on + strand at 1997.339 kb on + strand at 1997.342 kb on - strand at 1997.342 kb on - strand at 1997.347 kb on - strand at 1997.347 kb on - strand at 1997.347 kb on - strand at 1997.361 kb on + strand at 1997.361 kb on + strand at 1997.361 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction EMM_noCarbon with Sucrose and Putrescine remove 1,994,633 - -0.3 1,994,658 - -0.8 1,994,668 - +0.3 1,994,674 - +0.2 1,994,682 - +0.6 1,994,692 - GFF1970 0.10 -0.2 1,994,708 - GFF1970 0.13 -1.1 1,994,710 + GFF1970 0.13 +0.1 1,994,710 + GFF1970 0.13 -1.3 1,994,718 - GFF1970 0.14 +0.7 1,994,718 - GFF1970 0.14 +0.6 1,994,718 - GFF1970 0.14 -0.4 1,994,718 - GFF1970 0.14 +0.3 1,994,718 - GFF1970 0.14 +0.0 1,994,817 - GFF1970 0.30 +0.6 1,994,885 - GFF1970 0.40 -0.4 1,994,949 - GFF1970 0.50 -1.6 1,994,954 - GFF1970 0.51 +0.3 1,994,959 - GFF1970 0.52 -0.3 1,994,959 - GFF1970 0.52 -0.3 1,995,004 - GFF1970 0.59 -0.3 1,995,021 - GFF1970 0.62 -0.4 1,995,021 - GFF1970 0.62 +1.0 1,995,021 - GFF1970 0.62 -1.4 1,995,021 - GFF1970 0.62 +0.3 1,995,021 - GFF1970 0.62 +0.4 1,995,021 - GFF1970 0.62 -0.8 1,995,021 - GFF1970 0.62 -0.6 1,995,021 - GFF1970 0.62 +0.9 1,995,037 - GFF1970 0.64 -1.0 1,995,037 - GFF1970 0.64 -0.5 1,995,037 - GFF1970 0.64 -0.4 1,995,048 - GFF1970 0.66 +0.6 1,995,058 - GFF1970 0.67 -0.7 1,995,060 - GFF1970 0.68 +0.4 1,995,079 - GFF1970 0.71 +0.7 1,995,111 - GFF1970 0.76 +1.5 1,995,111 - GFF1970 0.76 -0.8 1,995,121 - GFF1970 0.77 +0.2 1,995,130 - GFF1970 0.79 -0.8 1,995,270 - -2.3 1,995,291 - +0.3 1,995,302 - -1.4 1,995,302 - -1.0 1,995,302 - -1.8 1,995,302 - -0.1 1,995,335 + -1.6 1,995,348 - -0.4 1,995,354 + -0.2 1,995,362 - +0.8 1,995,367 - -3.0 1,995,388 + -1.4 1,995,388 + +0.7 1,995,388 + -0.5 1,995,388 + +0.4 1,995,388 + -1.1 1,995,388 + +0.6 1,995,388 + +0.3 1,995,393 + -0.7 1,996,337 - -3.0 1,996,337 - -1.7 1,996,347 + +0.5 1,996,347 + -1.0 1,996,355 - +0.5 1,996,358 + -2.4 1,996,366 + +0.1 1,996,401 + -2.0 1,996,401 + -2.1 1,996,409 - -1.3 1,996,410 - -2.5 1,996,411 + -2.3 1,996,429 - +0.6 1,996,430 - -1.8 1,996,440 - -1.3 1,996,455 - -2.1 1,996,459 + -1.5 1,996,468 + GFF1972 0.10 -2.4 1,996,474 - GFF1972 0.11 -1.3 1,996,488 + GFF1972 0.12 -2.7 1,996,514 - GFF1972 0.15 -2.8 1,996,521 + GFF1972 0.16 -1.8 1,996,523 + GFF1972 0.16 -2.3 1,996,523 + GFF1972 0.16 -1.9 1,996,523 + GFF1972 0.16 -2.0 1,996,523 + GFF1972 0.16 -2.0 1,996,523 + GFF1972 0.16 -2.3 1,996,523 + GFF1972 0.16 -2.3 1,996,528 + GFF1972 0.16 -1.1 1,996,528 + GFF1972 0.16 -2.1 1,996,528 + GFF1972 0.16 -2.5 1,996,528 + GFF1972 0.16 -1.8 1,996,528 + GFF1972 0.16 -1.4 1,996,528 + GFF1972 0.16 -2.8 1,996,528 + GFF1972 0.16 -1.1 1,996,531 - GFF1972 0.17 -1.7 1,996,531 - GFF1972 0.17 -1.8 1,996,536 - GFF1972 0.17 -2.1 1,996,536 - GFF1972 0.17 -0.8 1,996,536 - GFF1972 0.17 -1.6 1,996,564 + GFF1972 0.20 -2.4 1,996,564 + GFF1972 0.20 -1.1 1,996,572 - GFF1972 0.21 -1.5 1,996,594 - GFF1972 0.23 -1.8 1,996,610 + GFF1972 0.25 -1.7 1,996,613 + GFF1972 0.25 -1.4 1,996,613 + GFF1972 0.25 -2.4 1,996,616 - GFF1972 0.25 -1.7 1,996,618 + GFF1972 0.25 -1.7 1,996,621 - GFF1972 0.26 -2.1 1,996,621 - GFF1972 0.26 -1.8 1,996,684 - GFF1972 0.32 -1.6 1,996,703 + GFF1972 0.34 -1.4 1,996,748 + GFF1972 0.39 -1.1 1,996,767 + GFF1972 0.41 +0.4 1,996,772 + GFF1972 0.41 -1.8 1,996,772 + GFF1972 0.41 -2.0 1,996,775 - GFF1972 0.42 -1.4 1,996,775 - GFF1972 0.42 -1.5 1,996,777 + GFF1972 0.42 -2.9 1,996,780 - GFF1972 0.42 -2.2 1,996,785 - GFF1972 0.43 -1.6 1,996,815 - GFF1972 0.46 -0.5 1,996,818 + GFF1972 0.46 -1.8 1,996,820 - GFF1972 0.46 -1.1 1,996,908 - GFF1972 0.55 +0.5 1,996,954 - GFF1972 0.60 -1.1 1,996,955 + GFF1972 0.60 -3.3 1,996,955 + GFF1972 0.60 -3.1 1,996,963 - GFF1972 0.61 -1.3 1,996,974 - GFF1972 0.62 -3.0 1,996,986 - GFF1972 0.63 -1.6 1,996,986 - GFF1972 0.63 -1.7 1,997,037 + GFF1972 0.68 -1.3 1,997,045 + GFF1972 0.69 -1.6 1,997,045 + GFF1972 0.69 -2.1 1,997,045 + GFF1972 0.69 -1.9 1,997,045 + GFF1972 0.69 -1.7 1,997,045 + GFF1972 0.69 -1.3 1,997,045 + GFF1972 0.69 -2.3 1,997,045 - GFF1972 0.69 -2.9 1,997,053 - GFF1972 0.70 -1.7 1,997,142 - GFF1972 0.79 -1.7 1,997,147 + GFF1972 0.80 -1.3 1,997,147 + GFF1972 0.80 -1.7 1,997,147 + GFF1972 0.80 -1.9 1,997,147 + GFF1972 0.80 -2.1 1,997,152 + GFF1972 0.80 -1.1 1,997,152 + GFF1972 0.80 -1.8 1,997,155 - GFF1972 0.80 +0.5 1,997,155 - GFF1972 0.80 +1.1 1,997,179 + GFF1972 0.83 -2.0 1,997,180 + GFF1972 0.83 -0.5 1,997,190 + GFF1972 0.84 -1.8 1,997,198 + GFF1972 0.85 -1.3 1,997,198 - GFF1972 0.85 +0.1 1,997,203 + GFF1972 0.85 -1.4 1,997,203 + GFF1972 0.85 -2.8 1,997,215 + GFF1972 0.87 -2.4 1,997,215 + GFF1972 0.87 -1.9 1,997,215 + GFF1972 0.87 +0.2 1,997,215 + GFF1972 0.87 -1.8 1,997,223 - GFF1972 0.87 -0.5 1,997,235 - GFF1972 0.89 -1.3 1,997,252 + -1.5 1,997,260 - -1.1 1,997,278 + -2.3 1,997,287 + -2.5 1,997,291 - -2.4 1,997,292 + -2.4 1,997,316 + +0.3 1,997,324 + -1.8 1,997,329 + -2.0 1,997,339 + -0.8 1,997,339 + -1.1 1,997,342 - -1.4 1,997,342 - +0.7 1,997,347 - +0.5 1,997,347 - -1.3 1,997,347 - -0.9 1,997,361 + -0.9 1,997,361 + +1.2 1,997,361 + -1.9
Or see this region's nucleotide sequence