Experiment: EMM_noCarbon with Sucrose and Putrescine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1947 and GFF1948 overlap by 4 nucleotides GFF1948 and GFF1949 are separated by 38 nucleotides
GFF1947 - UDP-N-acetylglucosamine--N-acetylmuramyl- (pentapeptide) pyrophosphoryl-undecaprenol N-acetylglucosamine transferase (EC 2.4.1.227), at 1,972,116 to 1,973,282
GFF1947
GFF1948 - Peptidoglycan glycosyltransferase FtsW (EC 2.4.1.129), at 1,973,279 to 1,974,505
GFF1948
GFF1949 - UDP-N-acetylmuramoyl-L-alanine--D-glutamate ligase (EC 6.3.2.9), at 1,974,544 to 1,975,857
GFF1949
Position (kb)
1973
1974
1975 Strain fitness (log2 ratio)
-2
-1
0
1 at 1973.302 kb on - strand at 1973.302 kb on - strand at 1973.330 kb on - strand at 1973.330 kb on - strand at 1973.330 kb on - strand at 1974.461 kb on - strand at 1974.463 kb on - strand at 1974.479 kb on - strand at 1974.479 kb on - strand at 1974.479 kb on - strand at 1974.484 kb on - strand at 1974.484 kb on - strand at 1974.500 kb on + strand at 1974.527 kb on + strand at 1974.527 kb on + strand at 1974.527 kb on + strand at 1974.527 kb on + strand at 1974.535 kb on - strand at 1974.535 kb on - strand at 1974.535 kb on - strand at 1974.535 kb on - strand at 1974.535 kb on - strand at 1974.535 kb on - strand at 1974.535 kb on - strand at 1974.535 kb on - strand at 1974.535 kb on - strand at 1974.535 kb on - strand at 1974.535 kb on - strand at 1974.535 kb on - strand at 1974.535 kb on - strand at 1974.549 kb on - strand at 1974.550 kb on - strand at 1974.554 kb on - strand at 1974.554 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction EMM_noCarbon with Sucrose and Putrescine remove 1,973,302 - +0.0 1,973,302 - -2.0 1,973,330 - -1.4 1,973,330 - +0.3 1,973,330 - +0.7 1,974,461 - -0.3 1,974,463 - -0.9 1,974,479 - +0.7 1,974,479 - -0.4 1,974,479 - +0.1 1,974,484 - +0.7 1,974,484 - -0.2 1,974,500 + +0.2 1,974,527 + -0.3 1,974,527 + -0.8 1,974,527 + -2.1 1,974,527 + -0.0 1,974,535 - -0.1 1,974,535 - -0.2 1,974,535 - +1.1 1,974,535 - -0.3 1,974,535 - +0.2 1,974,535 - -1.5 1,974,535 - -0.6 1,974,535 - +0.5 1,974,535 - -0.3 1,974,535 - -2.1 1,974,535 - +0.1 1,974,535 - -1.1 1,974,535 - +0.5 1,974,549 - +0.0 1,974,550 - +0.1 1,974,554 - -0.2 1,974,554 - -1.0
Or see this region's nucleotide sequence