Experiment: EMM_noCarbon with Sucrose and Putrescine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1641 and GFF1642 are separated by 138 nucleotides GFF1642 and GFF1643 are separated by 72 nucleotides GFF1643 and GFF1644 are separated by 88 nucleotides GFF1644 and GFF1645 are separated by 146 nucleotides GFF1645 and GFF1646 overlap by 4 nucleotides GFF1646 and GFF1647 are separated by 175 nucleotides
GFF1641 - tRNA-Ala-GGC, at 1,669,567 to 1,669,642
GFF1641
GFF1642 - tRNA-Ala-GGC, at 1,669,781 to 1,669,856
GFF1642
GFF1643 - tRNA-Ala-GGC, at 1,669,929 to 1,670,004
GFF1643
GFF1644 - hypothetical protein, at 1,670,093 to 1,670,431
GFF1644
GFF1645 - 3-phosphoshikimate 1-carboxyvinyltransferase (EC 2.5.1.19), at 1,670,578 to 1,671,972
GFF1645
GFF1646 - Cytidylate kinase (EC 2.7.4.25), at 1,671,969 to 1,672,598
GFF1646
GFF1647 - SSU ribosomal protein S1p, at 1,672,774 to 1,674,486
GFF1647
Position (kb)
1670
1671
1672 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 1669.631 kb on + strand, within GFF1641 at 1669.638 kb on + strand at 1669.674 kb on + strand at 1669.674 kb on + strand at 1669.682 kb on - strand at 1669.682 kb on - strand at 1669.705 kb on + strand at 1669.841 kb on + strand, within GFF1642 at 1669.845 kb on + strand, within GFF1642 at 1669.897 kb on - strand at 1669.908 kb on - strand at 1669.943 kb on - strand, within GFF1643 at 1670.264 kb on - strand, within GFF1644 at 1670.368 kb on + strand, within GFF1644 at 1670.421 kb on + strand at 1670.445 kb on - strand at 1670.539 kb on - strand at 1670.539 kb on - strand at 1670.539 kb on - strand at 1670.575 kb on + strand at 1670.579 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction EMM_noCarbon with Sucrose and Putrescine remove 1,669,631 + GFF1641 0.84 -1.5 1,669,638 + -0.7 1,669,674 + -1.9 1,669,674 + +0.8 1,669,682 - +2.8 1,669,682 - -1.2 1,669,705 + +0.2 1,669,841 + GFF1642 0.79 +1.4 1,669,845 + GFF1642 0.84 +0.4 1,669,897 - +0.6 1,669,908 - -0.6 1,669,943 - GFF1643 0.18 +0.4 1,670,264 - GFF1644 0.50 +1.3 1,670,368 + GFF1644 0.81 +0.5 1,670,421 + +0.5 1,670,445 - +0.9 1,670,539 - -0.3 1,670,539 - -0.5 1,670,539 - -0.3 1,670,575 + +0.5 1,670,579 + -0.3
Or see this region's nucleotide sequence