Experiment: EMM_noCarbon with Sucrose and Putrescine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF102 and GFF103 are separated by 123 nucleotides GFF103 and GFF104 are separated by 82 nucleotides GFF104 and GFF105 are separated by 27 nucleotides
GFF102 - Proline iminopeptidase (EC 3.4.11.5), at 92,750 to 93,841
GFF102
GFF103 - Carboxynorspermidine synthase (EC 1.5.1.43), at 93,965 to 95,170
GFF103
GFF104 - Inner membrane protein, at 95,253 to 95,600
GFF104
GFF105 - ATP-dependent DNA ligase (EC 6.5.1.1) LigC, at 95,628 to 97,226
GFF105
Position (kb) 93
94
95
96 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 92.983 kb on - strand, within GFF102 at 92.996 kb on - strand, within GFF102 at 93.023 kb on + strand, within GFF102 at 93.023 kb on + strand, within GFF102 at 93.030 kb on + strand, within GFF102 at 93.044 kb on + strand, within GFF102 at 93.044 kb on + strand, within GFF102 at 93.046 kb on + strand, within GFF102 at 93.051 kb on + strand, within GFF102 at 93.051 kb on + strand, within GFF102 at 93.052 kb on + strand, within GFF102 at 93.052 kb on - strand, within GFF102 at 93.054 kb on - strand, within GFF102 at 93.054 kb on - strand, within GFF102 at 93.059 kb on - strand, within GFF102 at 93.060 kb on - strand, within GFF102 at 93.060 kb on - strand, within GFF102 at 93.091 kb on - strand, within GFF102 at 93.097 kb on + strand, within GFF102 at 93.123 kb on - strand, within GFF102 at 93.130 kb on + strand, within GFF102 at 93.143 kb on - strand, within GFF102 at 93.173 kb on - strand, within GFF102 at 93.227 kb on - strand, within GFF102 at 93.237 kb on + strand, within GFF102 at 93.250 kb on + strand, within GFF102 at 93.255 kb on + strand, within GFF102 at 93.255 kb on + strand, within GFF102 at 93.260 kb on + strand, within GFF102 at 93.260 kb on + strand, within GFF102 at 93.260 kb on + strand, within GFF102 at 93.260 kb on + strand, within GFF102 at 93.260 kb on + strand, within GFF102 at 93.260 kb on + strand, within GFF102 at 93.260 kb on + strand, within GFF102 at 93.260 kb on + strand, within GFF102 at 93.260 kb on + strand, within GFF102 at 93.260 kb on + strand, within GFF102 at 93.260 kb on + strand, within GFF102 at 93.260 kb on + strand, within GFF102 at 93.264 kb on + strand, within GFF102 at 93.268 kb on - strand, within GFF102 at 93.268 kb on - strand, within GFF102 at 93.268 kb on - strand, within GFF102 at 93.268 kb on - strand, within GFF102 at 93.268 kb on - strand, within GFF102 at 93.268 kb on - strand, within GFF102 at 93.268 kb on - strand, within GFF102 at 93.268 kb on - strand, within GFF102 at 93.268 kb on - strand, within GFF102 at 93.268 kb on - strand, within GFF102 at 93.268 kb on - strand, within GFF102 at 93.304 kb on - strand, within GFF102 at 93.318 kb on + strand, within GFF102 at 93.318 kb on + strand, within GFF102 at 93.318 kb on + strand, within GFF102 at 93.318 kb on + strand, within GFF102 at 93.318 kb on + strand, within GFF102 at 93.318 kb on + strand, within GFF102 at 93.318 kb on + strand, within GFF102 at 93.318 kb on + strand, within GFF102 at 93.323 kb on + strand, within GFF102 at 93.326 kb on - strand, within GFF102 at 93.326 kb on - strand, within GFF102 at 93.328 kb on + strand, within GFF102 at 93.331 kb on - strand, within GFF102 at 93.336 kb on - strand, within GFF102 at 93.341 kb on - strand, within GFF102 at 93.367 kb on + strand, within GFF102 at 93.373 kb on + strand, within GFF102 at 93.393 kb on + strand, within GFF102 at 93.393 kb on + strand, within GFF102 at 93.394 kb on - strand, within GFF102 at 93.394 kb on - strand, within GFF102 at 93.401 kb on - strand, within GFF102 at 93.403 kb on + strand, within GFF102 at 93.414 kb on + strand, within GFF102 at 93.422 kb on - strand, within GFF102 at 93.433 kb on + strand, within GFF102 at 93.463 kb on + strand, within GFF102 at 93.465 kb on + strand, within GFF102 at 93.466 kb on - strand, within GFF102 at 93.473 kb on - strand, within GFF102 at 93.484 kb on + strand, within GFF102 at 93.492 kb on - strand, within GFF102 at 93.506 kb on + strand, within GFF102 at 93.506 kb on + strand, within GFF102 at 93.506 kb on + strand, within GFF102 at 93.506 kb on + strand, within GFF102 at 93.506 kb on + strand, within GFF102 at 93.506 kb on + strand, within GFF102 at 93.506 kb on + strand, within GFF102 at 93.507 kb on - strand, within GFF102 at 93.514 kb on - strand, within GFF102 at 93.514 kb on - strand, within GFF102 at 93.514 kb on - strand, within GFF102 at 93.514 kb on - strand, within GFF102 at 93.514 kb on - strand, within GFF102 at 93.524 kb on - strand, within GFF102 at 93.529 kb on - strand, within GFF102 at 93.534 kb on - strand, within GFF102 at 93.534 kb on - strand, within GFF102 at 93.535 kb on + strand, within GFF102 at 93.545 kb on + strand, within GFF102 at 93.578 kb on + strand, within GFF102 at 93.586 kb on - strand, within GFF102 at 93.586 kb on - strand, within GFF102 at 93.592 kb on - strand, within GFF102 at 93.616 kb on + strand, within GFF102 at 93.616 kb on - strand, within GFF102 at 93.623 kb on - strand, within GFF102 at 93.623 kb on - strand, within GFF102 at 93.624 kb on - strand, within GFF102 at 93.625 kb on + strand, within GFF102 at 93.655 kb on + strand, within GFF102 at 93.668 kb on - strand, within GFF102 at 93.691 kb on - strand, within GFF102 at 93.716 kb on - strand, within GFF102 at 93.746 kb on + strand at 93.841 kb on - strand at 93.866 kb on - strand at 93.869 kb on + strand at 93.869 kb on + strand at 93.870 kb on + strand at 93.882 kb on + strand at 93.887 kb on + strand at 93.889 kb on - strand at 93.890 kb on - strand at 93.952 kb on - strand at 95.189 kb on - strand at 95.223 kb on - strand at 95.226 kb on - strand at 95.229 kb on + strand at 95.237 kb on - strand at 95.239 kb on + strand at 95.247 kb on - strand at 95.259 kb on - strand at 95.266 kb on + strand at 95.266 kb on + strand at 95.274 kb on - strand at 95.283 kb on + strand at 95.291 kb on - strand, within GFF104 at 95.291 kb on - strand, within GFF104 at 95.316 kb on + strand, within GFF104 at 95.316 kb on + strand, within GFF104 at 95.329 kb on - strand, within GFF104 at 95.334 kb on + strand, within GFF104 at 95.334 kb on + strand, within GFF104 at 95.342 kb on - strand, within GFF104 at 95.375 kb on - strand, within GFF104 at 95.375 kb on - strand, within GFF104 at 95.384 kb on - strand, within GFF104 at 95.409 kb on - strand, within GFF104 at 95.421 kb on + strand, within GFF104 at 95.433 kb on + strand, within GFF104 at 95.438 kb on + strand, within GFF104 at 95.438 kb on + strand, within GFF104 at 95.451 kb on + strand, within GFF104 at 95.451 kb on + strand, within GFF104 at 95.455 kb on + strand, within GFF104 at 95.463 kb on - strand, within GFF104 at 95.463 kb on - strand, within GFF104 at 95.463 kb on - strand, within GFF104 at 95.471 kb on - strand, within GFF104 at 95.481 kb on + strand, within GFF104 at 95.481 kb on + strand, within GFF104 at 95.482 kb on - strand, within GFF104 at 95.489 kb on - strand, within GFF104 at 95.489 kb on - strand, within GFF104 at 95.489 kb on - strand, within GFF104 at 95.489 kb on - strand, within GFF104 at 95.507 kb on + strand, within GFF104 at 95.570 kb on - strand at 95.576 kb on + strand at 95.651 kb on - strand at 95.659 kb on + strand at 95.659 kb on + strand at 95.667 kb on - strand at 95.694 kb on - strand at 95.728 kb on + strand at 95.745 kb on + strand at 95.745 kb on + strand at 95.745 kb on + strand at 95.745 kb on + strand at 95.745 kb on + strand at 95.792 kb on - strand, within GFF105 at 95.804 kb on + strand, within GFF105 at 95.821 kb on + strand, within GFF105 at 95.821 kb on + strand, within GFF105 at 95.821 kb on + strand, within GFF105 at 95.821 kb on + strand, within GFF105 at 95.821 kb on + strand, within GFF105 at 95.826 kb on + strand, within GFF105 at 95.829 kb on - strand, within GFF105 at 95.829 kb on - strand, within GFF105 at 95.853 kb on - strand, within GFF105 at 95.871 kb on - strand, within GFF105 at 95.875 kb on + strand, within GFF105 at 95.875 kb on + strand, within GFF105 at 95.882 kb on - strand, within GFF105 at 95.883 kb on - strand, within GFF105 at 95.883 kb on - strand, within GFF105 at 95.883 kb on - strand, within GFF105 at 95.909 kb on + strand, within GFF105 at 95.909 kb on + strand, within GFF105 at 95.917 kb on - strand, within GFF105 at 95.917 kb on - strand, within GFF105 at 95.917 kb on - strand, within GFF105 at 95.928 kb on + strand, within GFF105 at 95.936 kb on - strand, within GFF105 at 95.952 kb on + strand, within GFF105 at 95.957 kb on + strand, within GFF105 at 95.972 kb on + strand, within GFF105 at 95.972 kb on + strand, within GFF105 at 95.972 kb on + strand, within GFF105 at 95.979 kb on + strand, within GFF105 at 95.980 kb on - strand, within GFF105 at 95.980 kb on - strand, within GFF105 at 95.980 kb on - strand, within GFF105 at 95.998 kb on - strand, within GFF105 at 96.005 kb on - strand, within GFF105 at 96.019 kb on + strand, within GFF105 at 96.025 kb on - strand, within GFF105 at 96.029 kb on - strand, within GFF105 at 96.034 kb on + strand, within GFF105 at 96.042 kb on - strand, within GFF105 at 96.050 kb on + strand, within GFF105 at 96.058 kb on - strand, within GFF105 at 96.161 kb on + strand, within GFF105 at 96.161 kb on + strand, within GFF105 at 96.161 kb on + strand, within GFF105 at 96.162 kb on - strand, within GFF105 at 96.169 kb on - strand, within GFF105 at 96.169 kb on - strand, within GFF105 at 96.169 kb on - strand, within GFF105
Per-strain Table
Position Strand Gene LocusTag Fraction EMM_noCarbon with Sucrose and Putrescine remove 92,983 - GFF102 0.21 +0.4 92,996 - GFF102 0.23 +1.1 93,023 + GFF102 0.25 +0.2 93,023 + GFF102 0.25 -2.4 93,030 + GFF102 0.26 -0.4 93,044 + GFF102 0.27 -0.5 93,044 + GFF102 0.27 -1.0 93,046 + GFF102 0.27 +0.1 93,051 + GFF102 0.28 +0.4 93,051 + GFF102 0.28 -0.1 93,052 + GFF102 0.28 -0.8 93,052 - GFF102 0.28 +0.5 93,054 - GFF102 0.28 +0.7 93,054 - GFF102 0.28 -2.8 93,059 - GFF102 0.28 -0.7 93,060 - GFF102 0.28 -0.1 93,060 - GFF102 0.28 +0.1 93,091 - GFF102 0.31 -1.4 93,097 + GFF102 0.32 +0.6 93,123 - GFF102 0.34 -0.3 93,130 + GFF102 0.35 +1.0 93,143 - GFF102 0.36 +1.3 93,173 - GFF102 0.39 -1.4 93,227 - GFF102 0.44 +0.2 93,237 + GFF102 0.45 -0.5 93,250 + GFF102 0.46 -0.8 93,255 + GFF102 0.46 -1.3 93,255 + GFF102 0.46 +1.0 93,260 + GFF102 0.47 -0.9 93,260 + GFF102 0.47 -1.4 93,260 + GFF102 0.47 -0.1 93,260 + GFF102 0.47 +0.8 93,260 + GFF102 0.47 -0.3 93,260 + GFF102 0.47 +0.1 93,260 + GFF102 0.47 -0.9 93,260 + GFF102 0.47 +0.4 93,260 + GFF102 0.47 -2.0 93,260 + GFF102 0.47 +0.7 93,260 + GFF102 0.47 +0.2 93,260 + GFF102 0.47 -1.5 93,264 + GFF102 0.47 -0.5 93,268 - GFF102 0.47 -0.0 93,268 - GFF102 0.47 -0.7 93,268 - GFF102 0.47 -1.5 93,268 - GFF102 0.47 +0.3 93,268 - GFF102 0.47 -0.9 93,268 - GFF102 0.47 -1.2 93,268 - GFF102 0.47 -0.4 93,268 - GFF102 0.47 -0.5 93,268 - GFF102 0.47 -0.1 93,268 - GFF102 0.47 -0.3 93,268 - GFF102 0.47 -1.3 93,304 - GFF102 0.51 +1.0 93,318 + GFF102 0.52 +1.5 93,318 + GFF102 0.52 +0.5 93,318 + GFF102 0.52 -1.1 93,318 + GFF102 0.52 -1.6 93,318 + GFF102 0.52 -0.1 93,318 + GFF102 0.52 +0.8 93,318 + GFF102 0.52 -0.4 93,318 + GFF102 0.52 -1.3 93,323 + GFF102 0.52 +0.3 93,326 - GFF102 0.53 -0.3 93,326 - GFF102 0.53 -1.1 93,328 + GFF102 0.53 +0.2 93,331 - GFF102 0.53 +0.6 93,336 - GFF102 0.54 +0.7 93,341 - GFF102 0.54 +0.3 93,367 + GFF102 0.57 -1.8 93,373 + GFF102 0.57 -0.1 93,393 + GFF102 0.59 -1.6 93,393 + GFF102 0.59 -0.3 93,394 - GFF102 0.59 -2.0 93,394 - GFF102 0.59 +0.3 93,401 - GFF102 0.60 +0.1 93,403 + GFF102 0.60 -1.0 93,414 + GFF102 0.61 +0.3 93,422 - GFF102 0.62 -2.9 93,433 + GFF102 0.63 -0.6 93,463 + GFF102 0.65 -0.8 93,465 + GFF102 0.65 +0.0 93,466 - GFF102 0.66 +0.2 93,473 - GFF102 0.66 +0.2 93,484 + GFF102 0.67 +1.1 93,492 - GFF102 0.68 -0.3 93,506 + GFF102 0.69 -0.9 93,506 + GFF102 0.69 +0.5 93,506 + GFF102 0.69 -1.1 93,506 + GFF102 0.69 -1.6 93,506 + GFF102 0.69 -2.0 93,506 + GFF102 0.69 +0.3 93,506 + GFF102 0.69 -0.9 93,507 - GFF102 0.69 -1.3 93,514 - GFF102 0.70 -1.1 93,514 - GFF102 0.70 -0.1 93,514 - GFF102 0.70 -2.3 93,514 - GFF102 0.70 +1.3 93,514 - GFF102 0.70 -0.6 93,524 - GFF102 0.71 -1.1 93,529 - GFF102 0.71 +1.1 93,534 - GFF102 0.72 -0.7 93,534 - GFF102 0.72 +1.2 93,535 + GFF102 0.72 +1.5 93,545 + GFF102 0.73 -1.8 93,578 + GFF102 0.76 -1.1 93,586 - GFF102 0.77 -0.1 93,586 - GFF102 0.77 -0.3 93,592 - GFF102 0.77 +0.7 93,616 + GFF102 0.79 -1.9 93,616 - GFF102 0.79 +1.5 93,623 - GFF102 0.80 -0.2 93,623 - GFF102 0.80 -0.9 93,624 - GFF102 0.80 +0.7 93,625 + GFF102 0.80 -1.1 93,655 + GFF102 0.83 -0.4 93,668 - GFF102 0.84 -0.1 93,691 - GFF102 0.86 -0.1 93,716 - GFF102 0.88 +1.2 93,746 + +0.4 93,841 - -0.4 93,866 - +0.5 93,869 + -0.5 93,869 + -0.5 93,870 + +0.1 93,882 + -0.4 93,887 + -1.1 93,889 - -0.4 93,890 - -0.9 93,952 - -1.6 95,189 - -0.9 95,223 - -0.4 95,226 - +1.9 95,229 + +0.3 95,237 - -2.0 95,239 + -0.3 95,247 - +1.6 95,259 - -0.4 95,266 + +0.1 95,266 + +0.6 95,274 - +1.6 95,283 + -0.1 95,291 - GFF104 0.11 +0.1 95,291 - GFF104 0.11 +0.4 95,316 + GFF104 0.18 -1.8 95,316 + GFF104 0.18 +0.9 95,329 - GFF104 0.22 -2.3 95,334 + GFF104 0.23 -0.2 95,334 + GFF104 0.23 -0.1 95,342 - GFF104 0.26 +0.8 95,375 - GFF104 0.35 +0.9 95,375 - GFF104 0.35 -1.5 95,384 - GFF104 0.38 -0.9 95,409 - GFF104 0.45 -1.8 95,421 + GFF104 0.48 -3.2 95,433 + GFF104 0.52 -0.8 95,438 + GFF104 0.53 -1.1 95,438 + GFF104 0.53 +1.2 95,451 + GFF104 0.57 +0.7 95,451 + GFF104 0.57 -2.4 95,455 + GFF104 0.58 -0.3 95,463 - GFF104 0.60 +0.5 95,463 - GFF104 0.60 +0.7 95,463 - GFF104 0.60 +0.3 95,471 - GFF104 0.63 -0.7 95,481 + GFF104 0.66 +1.9 95,481 + GFF104 0.66 -0.3 95,482 - GFF104 0.66 -0.3 95,489 - GFF104 0.68 -0.5 95,489 - GFF104 0.68 -1.3 95,489 - GFF104 0.68 +0.6 95,489 - GFF104 0.68 +0.3 95,507 + GFF104 0.73 -3.4 95,570 - +0.7 95,576 + +0.9 95,651 - -0.1 95,659 + -0.1 95,659 + -1.3 95,667 - -2.4 95,694 - -1.1 95,728 + -0.8 95,745 + +0.2 95,745 + +0.2 95,745 + +0.6 95,745 + +0.3 95,745 + -0.6 95,792 - GFF105 0.10 -0.6 95,804 + GFF105 0.11 -2.9 95,821 + GFF105 0.12 -0.3 95,821 + GFF105 0.12 -0.5 95,821 + GFF105 0.12 -1.4 95,821 + GFF105 0.12 +0.0 95,821 + GFF105 0.12 -0.2 95,826 + GFF105 0.12 +0.5 95,829 - GFF105 0.13 -0.3 95,829 - GFF105 0.13 -0.4 95,853 - GFF105 0.14 +0.2 95,871 - GFF105 0.15 +0.4 95,875 + GFF105 0.15 -1.9 95,875 + GFF105 0.15 -1.0 95,882 - GFF105 0.16 +0.5 95,883 - GFF105 0.16 +0.1 95,883 - GFF105 0.16 -0.4 95,883 - GFF105 0.16 -0.6 95,909 + GFF105 0.18 -0.6 95,909 + GFF105 0.18 -2.0 95,917 - GFF105 0.18 -0.5 95,917 - GFF105 0.18 +1.0 95,917 - GFF105 0.18 -0.3 95,928 + GFF105 0.19 +0.3 95,936 - GFF105 0.19 +0.3 95,952 + GFF105 0.20 -1.3 95,957 + GFF105 0.21 -1.0 95,972 + GFF105 0.22 -1.4 95,972 + GFF105 0.22 +0.5 95,972 + GFF105 0.22 +0.2 95,979 + GFF105 0.22 -0.4 95,980 - GFF105 0.22 +0.5 95,980 - GFF105 0.22 -0.1 95,980 - GFF105 0.22 +1.2 95,998 - GFF105 0.23 +0.7 96,005 - GFF105 0.24 -1.3 96,019 + GFF105 0.24 -0.5 96,025 - GFF105 0.25 -0.9 96,029 - GFF105 0.25 -0.9 96,034 + GFF105 0.25 -0.2 96,042 - GFF105 0.26 -1.2 96,050 + GFF105 0.26 +0.7 96,058 - GFF105 0.27 -0.6 96,161 + GFF105 0.33 -1.3 96,161 + GFF105 0.33 -1.6 96,161 + GFF105 0.33 +0.1 96,162 - GFF105 0.33 -0.4 96,169 - GFF105 0.34 +0.2 96,169 - GFF105 0.34 -0.4 96,169 - GFF105 0.34 -0.2
Or see this region's nucleotide sequence