Experiment: R2A_PIPES with Nickel (II) chloride 424 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt natB and OKGIIK_16690 are separated by 182 nucleotides OKGIIK_16690 and folE are separated by 26 nucleotides folE and OKGIIK_16700 are separated by 79 nucleotides
OKGIIK_16685: natB - ABC transporter permease, at 3,661,704 to 3,662,906
natB
OKGIIK_16690: OKGIIK_16690 - EF-hand domain-containing protein, at 3,663,089 to 3,663,391
_16690
OKGIIK_16695: folE - GTP cyclohydrolase I FolE, at 3,663,418 to 3,664,020
folE
OKGIIK_16700: OKGIIK_16700 - Recombinase RmuC, at 3,664,100 to 3,665,542
_16700
Position (kb)
3663
3664 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3662.094 kb on + strand, within natB at 3662.094 kb on + strand, within natB at 3662.095 kb on - strand, within natB at 3662.158 kb on - strand, within natB at 3662.158 kb on - strand, within natB at 3662.292 kb on + strand, within natB at 3662.292 kb on + strand, within natB at 3662.328 kb on + strand, within natB at 3662.328 kb on + strand, within natB at 3662.328 kb on + strand, within natB at 3662.328 kb on + strand, within natB at 3662.328 kb on + strand, within natB at 3662.328 kb on + strand, within natB at 3662.329 kb on - strand, within natB at 3662.329 kb on - strand, within natB at 3662.329 kb on - strand, within natB at 3662.329 kb on - strand, within natB at 3662.329 kb on - strand, within natB at 3662.635 kb on - strand, within natB at 3662.652 kb on + strand, within natB at 3662.652 kb on + strand, within natB at 3662.652 kb on + strand, within natB at 3662.652 kb on + strand, within natB at 3662.653 kb on - strand, within natB at 3662.653 kb on - strand, within natB at 3662.653 kb on - strand, within natB at 3662.653 kb on - strand, within natB at 3662.653 kb on - strand, within natB at 3662.653 kb on - strand, within natB at 3662.653 kb on - strand, within natB at 3662.653 kb on - strand, within natB at 3662.733 kb on + strand, within natB at 3662.733 kb on + strand, within natB at 3662.733 kb on + strand, within natB at 3662.734 kb on - strand, within natB at 3662.734 kb on - strand, within natB at 3662.734 kb on - strand, within natB at 3662.999 kb on - strand at 3662.999 kb on - strand at 3663.070 kb on + strand at 3663.070 kb on + strand at 3663.070 kb on + strand at 3663.070 kb on + strand at 3663.070 kb on + strand at 3663.180 kb on + strand, within OKGIIK_16690 at 3663.180 kb on + strand, within OKGIIK_16690 at 3663.181 kb on - strand, within OKGIIK_16690 at 3663.181 kb on - strand, within OKGIIK_16690 at 3663.282 kb on + strand, within OKGIIK_16690 at 3663.282 kb on + strand, within OKGIIK_16690 at 3663.282 kb on + strand, within OKGIIK_16690 at 3663.282 kb on + strand, within OKGIIK_16690 at 3663.283 kb on - strand, within OKGIIK_16690 at 3663.283 kb on - strand, within OKGIIK_16690 at 3663.283 kb on - strand, within OKGIIK_16690 at 3663.283 kb on - strand, within OKGIIK_16690 at 3663.283 kb on - strand, within OKGIIK_16690 at 3663.291 kb on + strand, within OKGIIK_16690 at 3663.291 kb on + strand, within OKGIIK_16690 at 3663.291 kb on + strand, within OKGIIK_16690 at 3663.291 kb on + strand, within OKGIIK_16690 at 3663.291 kb on + strand, within OKGIIK_16690 at 3663.292 kb on - strand, within OKGIIK_16690 at 3663.292 kb on - strand, within OKGIIK_16690 at 3663.292 kb on - strand, within OKGIIK_16690 at 3663.292 kb on - strand, within OKGIIK_16690 at 3663.342 kb on + strand, within OKGIIK_16690 at 3663.342 kb on + strand, within OKGIIK_16690 at 3663.342 kb on + strand, within OKGIIK_16690 at 3664.055 kb on + strand at 3664.331 kb on + strand, within OKGIIK_16700 at 3664.331 kb on + strand, within OKGIIK_16700 at 3664.331 kb on + strand, within OKGIIK_16700 at 3664.331 kb on + strand, within OKGIIK_16700 at 3664.332 kb on - strand, within OKGIIK_16700 at 3664.332 kb on - strand, within OKGIIK_16700
Per-strain Table
Position Strand Gene LocusTag Fraction R2A_PIPES with Nickel (II) chloride 424 uM remove 3,662,094 + natB OKGIIK_16685 0.32 +2.2 3,662,094 + natB OKGIIK_16685 0.32 -0.3 3,662,095 - natB OKGIIK_16685 0.33 +0.5 3,662,158 - natB OKGIIK_16685 0.38 +0.5 3,662,158 - natB OKGIIK_16685 0.38 -0.5 3,662,292 + natB OKGIIK_16685 0.49 -0.5 3,662,292 + natB OKGIIK_16685 0.49 +0.8 3,662,328 + natB OKGIIK_16685 0.52 -2.0 3,662,328 + natB OKGIIK_16685 0.52 -1.5 3,662,328 + natB OKGIIK_16685 0.52 -0.8 3,662,328 + natB OKGIIK_16685 0.52 -1.1 3,662,328 + natB OKGIIK_16685 0.52 -1.1 3,662,328 + natB OKGIIK_16685 0.52 -0.5 3,662,329 - natB OKGIIK_16685 0.52 +0.1 3,662,329 - natB OKGIIK_16685 0.52 -1.1 3,662,329 - natB OKGIIK_16685 0.52 -2.1 3,662,329 - natB OKGIIK_16685 0.52 -0.2 3,662,329 - natB OKGIIK_16685 0.52 +0.5 3,662,635 - natB OKGIIK_16685 0.77 -0.5 3,662,652 + natB OKGIIK_16685 0.79 -0.5 3,662,652 + natB OKGIIK_16685 0.79 -2.6 3,662,652 + natB OKGIIK_16685 0.79 -0.7 3,662,652 + natB OKGIIK_16685 0.79 -1.0 3,662,653 - natB OKGIIK_16685 0.79 -0.5 3,662,653 - natB OKGIIK_16685 0.79 -0.2 3,662,653 - natB OKGIIK_16685 0.79 -2.3 3,662,653 - natB OKGIIK_16685 0.79 -0.3 3,662,653 - natB OKGIIK_16685 0.79 -1.3 3,662,653 - natB OKGIIK_16685 0.79 -2.2 3,662,653 - natB OKGIIK_16685 0.79 -1.6 3,662,653 - natB OKGIIK_16685 0.79 -0.1 3,662,733 + natB OKGIIK_16685 0.86 -1.5 3,662,733 + natB OKGIIK_16685 0.86 +1.1 3,662,733 + natB OKGIIK_16685 0.86 +0.0 3,662,734 - natB OKGIIK_16685 0.86 -1.7 3,662,734 - natB OKGIIK_16685 0.86 +0.8 3,662,734 - natB OKGIIK_16685 0.86 -1.1 3,662,999 - +0.5 3,662,999 - +0.2 3,663,070 + -1.3 3,663,070 + -2.5 3,663,070 + +0.0 3,663,070 + +0.4 3,663,070 + +0.3 3,663,180 + OKGIIK_16690 0.30 +0.0 3,663,180 + OKGIIK_16690 0.30 -0.2 3,663,181 - OKGIIK_16690 0.30 -2.0 3,663,181 - OKGIIK_16690 0.30 -2.0 3,663,282 + OKGIIK_16690 0.64 -2.0 3,663,282 + OKGIIK_16690 0.64 +1.8 3,663,282 + OKGIIK_16690 0.64 -0.8 3,663,282 + OKGIIK_16690 0.64 -1.1 3,663,283 - OKGIIK_16690 0.64 -0.8 3,663,283 - OKGIIK_16690 0.64 +0.5 3,663,283 - OKGIIK_16690 0.64 -0.2 3,663,283 - OKGIIK_16690 0.64 -0.7 3,663,283 - OKGIIK_16690 0.64 -1.9 3,663,291 + OKGIIK_16690 0.67 -0.4 3,663,291 + OKGIIK_16690 0.67 -0.8 3,663,291 + OKGIIK_16690 0.67 +0.1 3,663,291 + OKGIIK_16690 0.67 +0.0 3,663,291 + OKGIIK_16690 0.67 -0.1 3,663,292 - OKGIIK_16690 0.67 -1.5 3,663,292 - OKGIIK_16690 0.67 -1.0 3,663,292 - OKGIIK_16690 0.67 -0.8 3,663,292 - OKGIIK_16690 0.67 -1.3 3,663,342 + OKGIIK_16690 0.83 -2.2 3,663,342 + OKGIIK_16690 0.83 -1.0 3,663,342 + OKGIIK_16690 0.83 -0.7 3,664,055 + -0.3 3,664,331 + OKGIIK_16700 0.16 -0.1 3,664,331 + OKGIIK_16700 0.16 +0.7 3,664,331 + OKGIIK_16700 0.16 -1.7 3,664,331 + OKGIIK_16700 0.16 -0.7 3,664,332 - OKGIIK_16700 0.16 +0.6 3,664,332 - OKGIIK_16700 0.16 +1.2
Or see this region's nucleotide sequence