Experiment: R2A_PIPES with Nickel (II) chloride 424 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt OKGIIK_16670 and OKGIIK_16675 overlap by 4 nucleotides OKGIIK_16675 and natA overlap by 4 nucleotides natA and natB overlap by 4 nucleotides natB and OKGIIK_16690 are separated by 182 nucleotides OKGIIK_16690 and folE are separated by 26 nucleotides
OKGIIK_16670: OKGIIK_16670 - Transporter, at 3,659,229 to 3,660,806
_16670
OKGIIK_16675: OKGIIK_16675 - hypothetical protein, at 3,660,803 to 3,660,976
_16675
OKGIIK_16680: natA - ABC transporter, at 3,660,973 to 3,661,707
natA
OKGIIK_16685: natB - ABC transporter permease, at 3,661,704 to 3,662,906
natB
OKGIIK_16690: OKGIIK_16690 - EF-hand domain-containing protein, at 3,663,089 to 3,663,391
_16690
OKGIIK_16695: folE - GTP cyclohydrolase I FolE, at 3,663,418 to 3,664,020
folE
Position (kb)
3661
3662
3663 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3660.964 kb on + strand at 3660.964 kb on + strand at 3660.965 kb on - strand at 3660.965 kb on - strand at 3660.965 kb on - strand at 3661.114 kb on + strand, within natA at 3661.114 kb on + strand, within natA at 3661.114 kb on + strand, within natA at 3661.115 kb on - strand, within natA at 3661.228 kb on + strand, within natA at 3661.228 kb on + strand, within natA at 3661.229 kb on - strand, within natA at 3661.229 kb on - strand, within natA at 3661.229 kb on - strand, within natA at 3661.261 kb on + strand, within natA at 3661.261 kb on + strand, within natA at 3661.262 kb on - strand, within natA at 3661.262 kb on - strand, within natA at 3662.094 kb on + strand, within natB at 3662.094 kb on + strand, within natB at 3662.095 kb on - strand, within natB at 3662.158 kb on - strand, within natB at 3662.158 kb on - strand, within natB at 3662.292 kb on + strand, within natB at 3662.292 kb on + strand, within natB at 3662.328 kb on + strand, within natB at 3662.328 kb on + strand, within natB at 3662.328 kb on + strand, within natB at 3662.328 kb on + strand, within natB at 3662.328 kb on + strand, within natB at 3662.328 kb on + strand, within natB at 3662.329 kb on - strand, within natB at 3662.329 kb on - strand, within natB at 3662.329 kb on - strand, within natB at 3662.329 kb on - strand, within natB at 3662.329 kb on - strand, within natB at 3662.635 kb on - strand, within natB at 3662.652 kb on + strand, within natB at 3662.652 kb on + strand, within natB at 3662.652 kb on + strand, within natB at 3662.652 kb on + strand, within natB at 3662.653 kb on - strand, within natB at 3662.653 kb on - strand, within natB at 3662.653 kb on - strand, within natB at 3662.653 kb on - strand, within natB at 3662.653 kb on - strand, within natB at 3662.653 kb on - strand, within natB at 3662.653 kb on - strand, within natB at 3662.653 kb on - strand, within natB at 3662.733 kb on + strand, within natB at 3662.733 kb on + strand, within natB at 3662.733 kb on + strand, within natB at 3662.734 kb on - strand, within natB at 3662.734 kb on - strand, within natB at 3662.734 kb on - strand, within natB at 3662.999 kb on - strand at 3662.999 kb on - strand at 3663.070 kb on + strand at 3663.070 kb on + strand at 3663.070 kb on + strand at 3663.070 kb on + strand at 3663.070 kb on + strand at 3663.180 kb on + strand, within OKGIIK_16690 at 3663.180 kb on + strand, within OKGIIK_16690 at 3663.181 kb on - strand, within OKGIIK_16690 at 3663.181 kb on - strand, within OKGIIK_16690 at 3663.282 kb on + strand, within OKGIIK_16690 at 3663.282 kb on + strand, within OKGIIK_16690 at 3663.282 kb on + strand, within OKGIIK_16690 at 3663.282 kb on + strand, within OKGIIK_16690 at 3663.283 kb on - strand, within OKGIIK_16690 at 3663.283 kb on - strand, within OKGIIK_16690 at 3663.283 kb on - strand, within OKGIIK_16690 at 3663.283 kb on - strand, within OKGIIK_16690 at 3663.283 kb on - strand, within OKGIIK_16690 at 3663.291 kb on + strand, within OKGIIK_16690 at 3663.291 kb on + strand, within OKGIIK_16690 at 3663.291 kb on + strand, within OKGIIK_16690 at 3663.291 kb on + strand, within OKGIIK_16690 at 3663.291 kb on + strand, within OKGIIK_16690 at 3663.292 kb on - strand, within OKGIIK_16690 at 3663.292 kb on - strand, within OKGIIK_16690 at 3663.292 kb on - strand, within OKGIIK_16690 at 3663.292 kb on - strand, within OKGIIK_16690 at 3663.342 kb on + strand, within OKGIIK_16690 at 3663.342 kb on + strand, within OKGIIK_16690 at 3663.342 kb on + strand, within OKGIIK_16690
Per-strain Table
Position Strand Gene LocusTag Fraction R2A_PIPES with Nickel (II) chloride 424 uM remove 3,660,964 + -1.1 3,660,964 + +0.6 3,660,965 - -0.5 3,660,965 - -2.7 3,660,965 - -0.8 3,661,114 + natA OKGIIK_16680 0.19 +0.1 3,661,114 + natA OKGIIK_16680 0.19 +0.2 3,661,114 + natA OKGIIK_16680 0.19 -0.3 3,661,115 - natA OKGIIK_16680 0.19 -0.4 3,661,228 + natA OKGIIK_16680 0.35 +0.6 3,661,228 + natA OKGIIK_16680 0.35 -1.8 3,661,229 - natA OKGIIK_16680 0.35 -1.5 3,661,229 - natA OKGIIK_16680 0.35 +0.2 3,661,229 - natA OKGIIK_16680 0.35 -1.3 3,661,261 + natA OKGIIK_16680 0.39 +1.1 3,661,261 + natA OKGIIK_16680 0.39 -1.1 3,661,262 - natA OKGIIK_16680 0.39 -0.8 3,661,262 - natA OKGIIK_16680 0.39 +0.3 3,662,094 + natB OKGIIK_16685 0.32 +2.2 3,662,094 + natB OKGIIK_16685 0.32 -0.3 3,662,095 - natB OKGIIK_16685 0.33 +0.5 3,662,158 - natB OKGIIK_16685 0.38 +0.5 3,662,158 - natB OKGIIK_16685 0.38 -0.5 3,662,292 + natB OKGIIK_16685 0.49 -0.5 3,662,292 + natB OKGIIK_16685 0.49 +0.8 3,662,328 + natB OKGIIK_16685 0.52 -2.0 3,662,328 + natB OKGIIK_16685 0.52 -1.5 3,662,328 + natB OKGIIK_16685 0.52 -0.8 3,662,328 + natB OKGIIK_16685 0.52 -1.1 3,662,328 + natB OKGIIK_16685 0.52 -1.1 3,662,328 + natB OKGIIK_16685 0.52 -0.5 3,662,329 - natB OKGIIK_16685 0.52 +0.1 3,662,329 - natB OKGIIK_16685 0.52 -1.1 3,662,329 - natB OKGIIK_16685 0.52 -2.1 3,662,329 - natB OKGIIK_16685 0.52 -0.2 3,662,329 - natB OKGIIK_16685 0.52 +0.5 3,662,635 - natB OKGIIK_16685 0.77 -0.5 3,662,652 + natB OKGIIK_16685 0.79 -0.5 3,662,652 + natB OKGIIK_16685 0.79 -2.6 3,662,652 + natB OKGIIK_16685 0.79 -0.7 3,662,652 + natB OKGIIK_16685 0.79 -1.0 3,662,653 - natB OKGIIK_16685 0.79 -0.5 3,662,653 - natB OKGIIK_16685 0.79 -0.2 3,662,653 - natB OKGIIK_16685 0.79 -2.3 3,662,653 - natB OKGIIK_16685 0.79 -0.3 3,662,653 - natB OKGIIK_16685 0.79 -1.3 3,662,653 - natB OKGIIK_16685 0.79 -2.2 3,662,653 - natB OKGIIK_16685 0.79 -1.6 3,662,653 - natB OKGIIK_16685 0.79 -0.1 3,662,733 + natB OKGIIK_16685 0.86 -1.5 3,662,733 + natB OKGIIK_16685 0.86 +1.1 3,662,733 + natB OKGIIK_16685 0.86 +0.0 3,662,734 - natB OKGIIK_16685 0.86 -1.7 3,662,734 - natB OKGIIK_16685 0.86 +0.8 3,662,734 - natB OKGIIK_16685 0.86 -1.1 3,662,999 - +0.5 3,662,999 - +0.2 3,663,070 + -1.3 3,663,070 + -2.5 3,663,070 + +0.0 3,663,070 + +0.4 3,663,070 + +0.3 3,663,180 + OKGIIK_16690 0.30 +0.0 3,663,180 + OKGIIK_16690 0.30 -0.2 3,663,181 - OKGIIK_16690 0.30 -2.0 3,663,181 - OKGIIK_16690 0.30 -2.0 3,663,282 + OKGIIK_16690 0.64 -2.0 3,663,282 + OKGIIK_16690 0.64 +1.8 3,663,282 + OKGIIK_16690 0.64 -0.8 3,663,282 + OKGIIK_16690 0.64 -1.1 3,663,283 - OKGIIK_16690 0.64 -0.8 3,663,283 - OKGIIK_16690 0.64 +0.5 3,663,283 - OKGIIK_16690 0.64 -0.2 3,663,283 - OKGIIK_16690 0.64 -0.7 3,663,283 - OKGIIK_16690 0.64 -1.9 3,663,291 + OKGIIK_16690 0.67 -0.4 3,663,291 + OKGIIK_16690 0.67 -0.8 3,663,291 + OKGIIK_16690 0.67 +0.1 3,663,291 + OKGIIK_16690 0.67 +0.0 3,663,291 + OKGIIK_16690 0.67 -0.1 3,663,292 - OKGIIK_16690 0.67 -1.5 3,663,292 - OKGIIK_16690 0.67 -1.0 3,663,292 - OKGIIK_16690 0.67 -0.8 3,663,292 - OKGIIK_16690 0.67 -1.3 3,663,342 + OKGIIK_16690 0.83 -2.2 3,663,342 + OKGIIK_16690 0.83 -1.0 3,663,342 + OKGIIK_16690 0.83 -0.7
Or see this region's nucleotide sequence