Experiment: R2A_PIPES with Nickel (II) chloride 424 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt pyrE and xth are separated by 165 nucleotides xth and OKGIIK_15695 overlap by 11 nucleotides OKGIIK_15695 and OKGIIK_15700 are separated by 137 nucleotides OKGIIK_15700 and OKGIIK_15705 overlap by 31 nucleotides
OKGIIK_15685: pyrE - orotate phosphoribosyltransferase, at 3,426,291 to 3,426,938
pyrE
OKGIIK_15690: xth - exodeoxyribonuclease III, at 3,427,104 to 3,427,874
xth
OKGIIK_15695: OKGIIK_15695 - MFS transporter, at 3,427,864 to 3,429,180
_15695
OKGIIK_15700: OKGIIK_15700 - hypothetical protein, at 3,429,318 to 3,429,629
_15700
OKGIIK_15705: OKGIIK_15705 - anhydro-N-acetylmuramic acid kinase, at 3,429,599 to 3,430,729
_15705
Position (kb)
3427
3428
3429
3430 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3427.008 kb on + strand at 3427.008 kb on + strand at 3427.008 kb on + strand at 3427.008 kb on + strand at 3427.008 kb on + strand at 3427.008 kb on + strand at 3427.009 kb on - strand at 3427.009 kb on - strand at 3427.009 kb on - strand at 3427.066 kb on + strand at 3427.066 kb on + strand at 3427.067 kb on - strand at 3427.075 kb on + strand at 3427.075 kb on + strand at 3427.075 kb on + strand at 3427.075 kb on + strand at 3427.075 kb on + strand at 3427.075 kb on + strand at 3427.075 kb on + strand at 3427.075 kb on + strand at 3427.075 kb on + strand at 3427.075 kb on + strand at 3427.075 kb on + strand at 3427.075 kb on + strand at 3427.075 kb on + strand at 3427.075 kb on + strand at 3427.076 kb on - strand at 3427.076 kb on - strand at 3427.076 kb on - strand at 3427.076 kb on - strand at 3427.076 kb on - strand at 3427.076 kb on - strand at 3427.076 kb on - strand at 3427.076 kb on - strand at 3427.076 kb on - strand at 3427.076 kb on - strand at 3427.076 kb on - strand at 3427.272 kb on + strand, within xth at 3427.272 kb on + strand, within xth at 3427.272 kb on + strand, within xth at 3427.273 kb on - strand, within xth at 3427.299 kb on + strand, within xth at 3427.299 kb on + strand, within xth at 3427.300 kb on - strand, within xth at 3427.317 kb on + strand, within xth at 3427.317 kb on + strand, within xth at 3427.317 kb on + strand, within xth at 3427.342 kb on + strand, within xth at 3427.342 kb on + strand, within xth at 3427.342 kb on + strand, within xth at 3427.342 kb on + strand, within xth at 3427.343 kb on - strand, within xth at 3427.343 kb on - strand, within xth at 3427.343 kb on - strand, within xth at 3427.386 kb on + strand, within xth at 3427.386 kb on + strand, within xth at 3427.387 kb on - strand, within xth at 3427.387 kb on - strand, within xth at 3427.428 kb on + strand, within xth at 3427.428 kb on + strand, within xth at 3427.428 kb on + strand, within xth at 3427.428 kb on + strand, within xth at 3427.428 kb on + strand, within xth at 3427.428 kb on + strand, within xth at 3427.429 kb on - strand, within xth at 3427.429 kb on - strand, within xth at 3427.429 kb on - strand, within xth at 3427.429 kb on - strand, within xth at 3427.429 kb on - strand, within xth at 3427.429 kb on - strand, within xth at 3427.533 kb on + strand, within xth at 3427.534 kb on - strand, within xth at 3427.707 kb on + strand, within xth at 3427.707 kb on + strand, within xth at 3427.707 kb on + strand, within xth at 3427.707 kb on + strand, within xth at 3427.707 kb on + strand, within xth at 3427.707 kb on + strand, within xth at 3427.708 kb on - strand, within xth at 3427.708 kb on - strand, within xth at 3427.708 kb on - strand, within xth at 3427.708 kb on - strand, within xth at 3427.708 kb on - strand, within xth at 3427.767 kb on + strand, within xth at 3427.819 kb on - strand at 3427.851 kb on + strand at 3427.851 kb on + strand at 3427.851 kb on + strand at 3427.851 kb on + strand at 3427.851 kb on + strand at 3427.851 kb on + strand at 3427.851 kb on + strand at 3427.851 kb on + strand at 3427.851 kb on + strand at 3427.852 kb on - strand at 3427.852 kb on - strand at 3427.852 kb on - strand at 3427.852 kb on - strand at 3427.852 kb on - strand at 3427.852 kb on - strand at 3427.852 kb on - strand at 3427.852 kb on - strand at 3427.853 kb on + strand at 3427.853 kb on + strand at 3427.853 kb on + strand at 3427.853 kb on + strand at 3427.853 kb on + strand at 3427.853 kb on + strand at 3427.853 kb on + strand at 3427.853 kb on + strand at 3427.853 kb on + strand at 3427.853 kb on + strand at 3427.853 kb on + strand at 3427.853 kb on + strand at 3427.853 kb on + strand at 3427.854 kb on - strand at 3427.854 kb on - strand at 3427.854 kb on - strand at 3427.854 kb on - strand at 3427.854 kb on - strand at 3427.854 kb on - strand at 3427.854 kb on - strand at 3427.854 kb on - strand at 3427.863 kb on + strand at 3427.863 kb on + strand at 3427.863 kb on + strand at 3427.887 kb on + strand at 3427.887 kb on + strand at 3427.888 kb on - strand at 3428.002 kb on + strand, within OKGIIK_15695 at 3428.002 kb on + strand, within OKGIIK_15695 at 3428.003 kb on - strand, within OKGIIK_15695 at 3428.003 kb on - strand, within OKGIIK_15695 at 3428.003 kb on - strand, within OKGIIK_15695 at 3428.068 kb on + strand, within OKGIIK_15695 at 3428.068 kb on + strand, within OKGIIK_15695 at 3428.068 kb on + strand, within OKGIIK_15695 at 3428.068 kb on + strand, within OKGIIK_15695 at 3428.068 kb on + strand, within OKGIIK_15695 at 3428.068 kb on + strand, within OKGIIK_15695 at 3428.068 kb on + strand, within OKGIIK_15695 at 3428.068 kb on + strand, within OKGIIK_15695 at 3428.068 kb on + strand, within OKGIIK_15695 at 3428.069 kb on - strand, within OKGIIK_15695 at 3428.069 kb on - strand, within OKGIIK_15695 at 3428.069 kb on - strand, within OKGIIK_15695 at 3428.069 kb on - strand, within OKGIIK_15695 at 3428.069 kb on - strand, within OKGIIK_15695 at 3428.069 kb on - strand, within OKGIIK_15695 at 3428.069 kb on - strand, within OKGIIK_15695 at 3428.069 kb on - strand, within OKGIIK_15695 at 3428.069 kb on - strand, within OKGIIK_15695 at 3428.069 kb on - strand, within OKGIIK_15695 at 3428.290 kb on + strand, within OKGIIK_15695 at 3428.341 kb on + strand, within OKGIIK_15695 at 3428.342 kb on - strand, within OKGIIK_15695 at 3428.426 kb on - strand, within OKGIIK_15695 at 3428.426 kb on - strand, within OKGIIK_15695 at 3428.426 kb on - strand, within OKGIIK_15695 at 3428.666 kb on - strand, within OKGIIK_15695 at 3428.716 kb on + strand, within OKGIIK_15695 at 3428.717 kb on - strand, within OKGIIK_15695 at 3428.833 kb on + strand, within OKGIIK_15695 at 3428.834 kb on - strand, within OKGIIK_15695 at 3428.834 kb on - strand, within OKGIIK_15695 at 3428.834 kb on - strand, within OKGIIK_15695 at 3428.901 kb on + strand, within OKGIIK_15695 at 3428.977 kb on + strand, within OKGIIK_15695 at 3428.977 kb on + strand, within OKGIIK_15695 at 3428.978 kb on - strand, within OKGIIK_15695 at 3429.068 kb on - strand at 3429.068 kb on - strand at 3429.068 kb on - strand at 3429.068 kb on - strand at 3429.130 kb on + strand at 3429.130 kb on + strand at 3429.131 kb on - strand at 3429.131 kb on - strand at 3429.131 kb on - strand at 3429.178 kb on + strand at 3429.178 kb on + strand at 3429.178 kb on + strand at 3429.179 kb on + strand at 3429.179 kb on - strand at 3429.179 kb on - strand at 3429.256 kb on - strand at 3429.300 kb on + strand at 3429.300 kb on + strand at 3429.300 kb on + strand at 3429.300 kb on + strand at 3429.300 kb on + strand at 3429.300 kb on + strand at 3429.300 kb on + strand at 3429.301 kb on - strand at 3429.301 kb on - strand at 3429.301 kb on - strand at 3429.301 kb on - strand at 3429.301 kb on - strand at 3429.317 kb on + strand at 3429.317 kb on + strand at 3429.317 kb on + strand at 3429.318 kb on - strand at 3429.318 kb on - strand at 3429.318 kb on - strand at 3429.341 kb on + strand at 3429.341 kb on + strand at 3429.341 kb on + strand at 3429.342 kb on - strand at 3429.342 kb on - strand at 3429.342 kb on - strand at 3429.600 kb on + strand at 3429.600 kb on + strand at 3429.600 kb on + strand at 3429.627 kb on + strand at 3429.627 kb on + strand at 3429.627 kb on + strand at 3429.628 kb on - strand at 3429.628 kb on - strand at 3429.628 kb on - strand at 3429.987 kb on + strand, within OKGIIK_15705 at 3429.988 kb on - strand, within OKGIIK_15705 at 3429.988 kb on - strand, within OKGIIK_15705 at 3430.026 kb on + strand, within OKGIIK_15705 at 3430.026 kb on + strand, within OKGIIK_15705 at 3430.027 kb on - strand, within OKGIIK_15705 at 3430.027 kb on - strand, within OKGIIK_15705 at 3430.084 kb on - strand, within OKGIIK_15705 at 3430.084 kb on - strand, within OKGIIK_15705
Per-strain Table
Position Strand Gene LocusTag Fraction R2A_PIPES with Nickel (II) chloride 424 uM remove 3,427,008 + -0.5 3,427,008 + +0.6 3,427,008 + +0.5 3,427,008 + -0.5 3,427,008 + -0.1 3,427,008 + -1.8 3,427,009 - -0.5 3,427,009 - +0.5 3,427,009 - -0.1 3,427,066 + -0.6 3,427,066 + +1.2 3,427,067 - -0.8 3,427,075 + -2.0 3,427,075 + -1.1 3,427,075 + +0.1 3,427,075 + +0.5 3,427,075 + +0.7 3,427,075 + -0.2 3,427,075 + -0.1 3,427,075 + -0.8 3,427,075 + -1.8 3,427,075 + +0.3 3,427,075 + -0.7 3,427,075 + -0.7 3,427,075 + +0.6 3,427,075 + -0.1 3,427,076 - -0.1 3,427,076 - +0.7 3,427,076 - -1.2 3,427,076 - -0.5 3,427,076 - +0.5 3,427,076 - -1.2 3,427,076 - -0.3 3,427,076 - -0.5 3,427,076 - -2.2 3,427,076 - -0.3 3,427,076 - +0.3 3,427,272 + xth OKGIIK_15690 0.22 +2.1 3,427,272 + xth OKGIIK_15690 0.22 +1.7 3,427,272 + xth OKGIIK_15690 0.22 -2.1 3,427,273 - xth OKGIIK_15690 0.22 -0.1 3,427,299 + xth OKGIIK_15690 0.25 -0.1 3,427,299 + xth OKGIIK_15690 0.25 -1.3 3,427,300 - xth OKGIIK_15690 0.25 -1.2 3,427,317 + xth OKGIIK_15690 0.28 -0.4 3,427,317 + xth OKGIIK_15690 0.28 -0.5 3,427,317 + xth OKGIIK_15690 0.28 -0.1 3,427,342 + xth OKGIIK_15690 0.31 +1.5 3,427,342 + xth OKGIIK_15690 0.31 -0.8 3,427,342 + xth OKGIIK_15690 0.31 -0.2 3,427,342 + xth OKGIIK_15690 0.31 -2.1 3,427,343 - xth OKGIIK_15690 0.31 +1.0 3,427,343 - xth OKGIIK_15690 0.31 -0.3 3,427,343 - xth OKGIIK_15690 0.31 +1.5 3,427,386 + xth OKGIIK_15690 0.37 -1.1 3,427,386 + xth OKGIIK_15690 0.37 -0.5 3,427,387 - xth OKGIIK_15690 0.37 -1.0 3,427,387 - xth OKGIIK_15690 0.37 -0.0 3,427,428 + xth OKGIIK_15690 0.42 -1.5 3,427,428 + xth OKGIIK_15690 0.42 -1.4 3,427,428 + xth OKGIIK_15690 0.42 -2.2 3,427,428 + xth OKGIIK_15690 0.42 +0.2 3,427,428 + xth OKGIIK_15690 0.42 -2.1 3,427,428 + xth OKGIIK_15690 0.42 -0.4 3,427,429 - xth OKGIIK_15690 0.42 -0.5 3,427,429 - xth OKGIIK_15690 0.42 -1.3 3,427,429 - xth OKGIIK_15690 0.42 +0.5 3,427,429 - xth OKGIIK_15690 0.42 +0.1 3,427,429 - xth OKGIIK_15690 0.42 -0.5 3,427,429 - xth OKGIIK_15690 0.42 +0.1 3,427,533 + xth OKGIIK_15690 0.56 +0.5 3,427,534 - xth OKGIIK_15690 0.56 +0.9 3,427,707 + xth OKGIIK_15690 0.78 -2.0 3,427,707 + xth OKGIIK_15690 0.78 -1.3 3,427,707 + xth OKGIIK_15690 0.78 -0.4 3,427,707 + xth OKGIIK_15690 0.78 +0.9 3,427,707 + xth OKGIIK_15690 0.78 +0.5 3,427,707 + xth OKGIIK_15690 0.78 -1.1 3,427,708 - xth OKGIIK_15690 0.78 -2.3 3,427,708 - xth OKGIIK_15690 0.78 -1.7 3,427,708 - xth OKGIIK_15690 0.78 -0.1 3,427,708 - xth OKGIIK_15690 0.78 -0.8 3,427,708 - xth OKGIIK_15690 0.78 -2.4 3,427,767 + xth OKGIIK_15690 0.86 -0.1 3,427,819 - -0.1 3,427,851 + +0.9 3,427,851 + -0.5 3,427,851 + -1.3 3,427,851 + -1.3 3,427,851 + +0.9 3,427,851 + -0.8 3,427,851 + -2.3 3,427,851 + -0.8 3,427,851 + +0.2 3,427,852 - +0.3 3,427,852 - -1.3 3,427,852 - -1.3 3,427,852 - -0.4 3,427,852 - +0.5 3,427,852 - +0.0 3,427,852 - +0.2 3,427,852 - -0.5 3,427,853 + -1.4 3,427,853 + -0.4 3,427,853 + +0.2 3,427,853 + +1.5 3,427,853 + +0.3 3,427,853 + -0.7 3,427,853 + -0.5 3,427,853 + -0.3 3,427,853 + -1.1 3,427,853 + +0.9 3,427,853 + -0.1 3,427,853 + +0.2 3,427,853 + -1.0 3,427,854 - -1.2 3,427,854 - -0.3 3,427,854 - -2.1 3,427,854 - -1.6 3,427,854 - +0.4 3,427,854 - -0.8 3,427,854 - +0.1 3,427,854 - -1.0 3,427,863 + -0.1 3,427,863 + -0.5 3,427,863 + -2.2 3,427,887 + -0.9 3,427,887 + -2.1 3,427,888 - -2.0 3,428,002 + OKGIIK_15695 0.10 -2.1 3,428,002 + OKGIIK_15695 0.10 -1.8 3,428,003 - OKGIIK_15695 0.11 -0.5 3,428,003 - OKGIIK_15695 0.11 -1.5 3,428,003 - OKGIIK_15695 0.11 -1.2 3,428,068 + OKGIIK_15695 0.15 -0.8 3,428,068 + OKGIIK_15695 0.15 -0.8 3,428,068 + OKGIIK_15695 0.15 -0.1 3,428,068 + OKGIIK_15695 0.15 -1.8 3,428,068 + OKGIIK_15695 0.15 -0.5 3,428,068 + OKGIIK_15695 0.15 -1.1 3,428,068 + OKGIIK_15695 0.15 +0.6 3,428,068 + OKGIIK_15695 0.15 -0.8 3,428,068 + OKGIIK_15695 0.15 -1.6 3,428,069 - OKGIIK_15695 0.16 -1.5 3,428,069 - OKGIIK_15695 0.16 +1.1 3,428,069 - OKGIIK_15695 0.16 -1.4 3,428,069 - OKGIIK_15695 0.16 -1.0 3,428,069 - OKGIIK_15695 0.16 -0.5 3,428,069 - OKGIIK_15695 0.16 -1.5 3,428,069 - OKGIIK_15695 0.16 -1.7 3,428,069 - OKGIIK_15695 0.16 +0.2 3,428,069 - OKGIIK_15695 0.16 -1.1 3,428,069 - OKGIIK_15695 0.16 -0.5 3,428,290 + OKGIIK_15695 0.32 -0.5 3,428,341 + OKGIIK_15695 0.36 +0.6 3,428,342 - OKGIIK_15695 0.36 -0.8 3,428,426 - OKGIIK_15695 0.43 -1.3 3,428,426 - OKGIIK_15695 0.43 -2.1 3,428,426 - OKGIIK_15695 0.43 -0.8 3,428,666 - OKGIIK_15695 0.61 -2.5 3,428,716 + OKGIIK_15695 0.65 -1.0 3,428,717 - OKGIIK_15695 0.65 -1.7 3,428,833 + OKGIIK_15695 0.74 -1.1 3,428,834 - OKGIIK_15695 0.74 -2.5 3,428,834 - OKGIIK_15695 0.74 -1.4 3,428,834 - OKGIIK_15695 0.74 -1.3 3,428,901 + OKGIIK_15695 0.79 -1.1 3,428,977 + OKGIIK_15695 0.85 -2.1 3,428,977 + OKGIIK_15695 0.85 -0.8 3,428,978 - OKGIIK_15695 0.85 -0.8 3,429,068 - -0.5 3,429,068 - -1.2 3,429,068 - -0.5 3,429,068 - -0.8 3,429,130 + -1.8 3,429,130 + +0.2 3,429,131 - -0.6 3,429,131 - +0.7 3,429,131 - -2.8 3,429,178 + -0.4 3,429,178 + -0.1 3,429,178 + -1.7 3,429,179 + +0.5 3,429,179 - -1.7 3,429,179 - +0.3 3,429,256 - -0.6 3,429,300 + +0.9 3,429,300 + +0.5 3,429,300 + -0.8 3,429,300 + +1.5 3,429,300 + -0.9 3,429,300 + +0.2 3,429,300 + -0.1 3,429,301 - -1.7 3,429,301 - +0.7 3,429,301 - +0.8 3,429,301 - +0.4 3,429,301 - -0.8 3,429,317 + -2.0 3,429,317 + +0.3 3,429,317 + -1.5 3,429,318 - -0.1 3,429,318 - +2.2 3,429,318 - +1.1 3,429,341 + -2.8 3,429,341 + +0.5 3,429,341 + +0.5 3,429,342 - +0.8 3,429,342 - -1.5 3,429,342 - +2.1 3,429,600 + -0.2 3,429,600 + +0.8 3,429,600 + -0.2 3,429,627 + -2.1 3,429,627 + -3.4 3,429,627 + -2.2 3,429,628 - -2.3 3,429,628 - -0.1 3,429,628 - -1.1 3,429,987 + OKGIIK_15705 0.34 -2.4 3,429,988 - OKGIIK_15705 0.34 -0.5 3,429,988 - OKGIIK_15705 0.34 +0.0 3,430,026 + OKGIIK_15705 0.38 -1.3 3,430,026 + OKGIIK_15705 0.38 -0.4 3,430,027 - OKGIIK_15705 0.38 +0.5 3,430,027 - OKGIIK_15705 0.38 -1.1 3,430,084 - OKGIIK_15705 0.43 -1.3 3,430,084 - OKGIIK_15705 0.43 -0.5
Or see this region's nucleotide sequence