Experiment: R2A_PIPES with Nickel (II) chloride 424 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt fepA and ompR are separated by 194 nucleotides ompR and baeS are separated by 3 nucleotides
OKGIIK_14880: fepA - TonB-dependent receptor, at 3,237,714 to 3,240,386
fepA
OKGIIK_14885: ompR - DNA-binding response regulator, at 3,240,581 to 3,241,270
ompR
OKGIIK_14890: baeS - two-component sensor histidine kinase, at 3,241,274 to 3,242,647
baeS
Position (kb)
3240
3241
3242 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3239.628 kb on + strand, within fepA at 3239.769 kb on + strand, within fepA at 3239.770 kb on - strand, within fepA at 3239.770 kb on - strand, within fepA at 3239.770 kb on - strand, within fepA at 3239.770 kb on - strand, within fepA at 3239.770 kb on - strand, within fepA at 3239.826 kb on + strand, within fepA at 3239.826 kb on + strand, within fepA at 3239.826 kb on + strand, within fepA at 3239.826 kb on + strand, within fepA at 3239.826 kb on + strand, within fepA at 3239.826 kb on + strand, within fepA at 3239.826 kb on + strand, within fepA at 3239.827 kb on - strand, within fepA at 3239.827 kb on - strand, within fepA at 3239.827 kb on - strand, within fepA at 3239.827 kb on - strand, within fepA at 3239.827 kb on - strand, within fepA at 3239.900 kb on + strand, within fepA at 3239.900 kb on + strand, within fepA at 3239.900 kb on + strand, within fepA at 3239.901 kb on - strand, within fepA at 3239.901 kb on - strand, within fepA at 3239.901 kb on - strand, within fepA at 3239.901 kb on - strand, within fepA at 3239.931 kb on + strand, within fepA at 3239.932 kb on - strand, within fepA at 3240.015 kb on + strand, within fepA at 3240.015 kb on + strand, within fepA at 3240.015 kb on + strand, within fepA at 3240.015 kb on + strand, within fepA at 3240.016 kb on - strand, within fepA at 3240.016 kb on - strand, within fepA at 3240.016 kb on - strand, within fepA at 3240.016 kb on - strand, within fepA at 3240.016 kb on - strand, within fepA at 3240.016 kb on - strand, within fepA at 3240.057 kb on + strand, within fepA at 3240.058 kb on - strand, within fepA at 3240.058 kb on - strand, within fepA at 3240.108 kb on + strand, within fepA at 3240.109 kb on - strand, within fepA at 3240.109 kb on - strand, within fepA at 3240.109 kb on - strand, within fepA at 3240.145 kb on - strand at 3240.198 kb on + strand at 3240.198 kb on + strand at 3240.198 kb on + strand at 3240.198 kb on + strand at 3240.199 kb on - strand at 3240.199 kb on - strand at 3240.199 kb on - strand at 3240.199 kb on - strand at 3240.238 kb on - strand at 3240.238 kb on - strand at 3240.264 kb on + strand at 3240.264 kb on + strand at 3240.264 kb on + strand at 3240.265 kb on - strand at 3240.265 kb on - strand at 3240.265 kb on - strand at 3240.265 kb on - strand at 3240.291 kb on - strand at 3240.345 kb on + strand at 3240.346 kb on - strand at 3240.357 kb on + strand at 3240.357 kb on + strand at 3240.357 kb on + strand at 3240.357 kb on + strand at 3240.357 kb on + strand at 3240.357 kb on + strand at 3240.357 kb on + strand at 3240.357 kb on + strand at 3240.357 kb on + strand at 3240.357 kb on + strand at 3240.357 kb on + strand at 3240.357 kb on + strand at 3240.357 kb on + strand at 3240.357 kb on + strand at 3240.358 kb on - strand at 3240.358 kb on - strand at 3240.358 kb on - strand at 3240.358 kb on - strand at 3240.358 kb on - strand at 3240.358 kb on - strand at 3240.358 kb on - strand at 3240.358 kb on - strand at 3240.358 kb on - strand at 3240.358 kb on - strand at 3240.623 kb on + strand at 3240.623 kb on + strand at 3240.623 kb on + strand at 3240.623 kb on + strand at 3240.624 kb on - strand at 3240.624 kb on - strand at 3240.624 kb on - strand at 3240.624 kb on - strand at 3240.624 kb on - strand at 3240.624 kb on - strand at 3240.624 kb on - strand at 3240.624 kb on - strand at 3240.625 kb on + strand at 3240.626 kb on - strand at 3240.626 kb on - strand at 3240.720 kb on + strand, within ompR at 3240.720 kb on + strand, within ompR at 3240.720 kb on + strand, within ompR at 3240.720 kb on + strand, within ompR at 3240.720 kb on + strand, within ompR at 3240.721 kb on - strand, within ompR at 3240.797 kb on - strand, within ompR at 3240.872 kb on + strand, within ompR at 3240.873 kb on - strand, within ompR at 3240.873 kb on - strand, within ompR at 3240.873 kb on - strand, within ompR at 3240.873 kb on - strand, within ompR at 3241.064 kb on + strand, within ompR at 3241.065 kb on - strand, within ompR at 3241.128 kb on - strand, within ompR at 3241.128 kb on - strand, within ompR at 3241.223 kb on - strand at 3241.241 kb on + strand at 3241.241 kb on + strand at 3241.241 kb on + strand at 3241.245 kb on + strand at 3241.245 kb on + strand at 3241.245 kb on + strand at 3241.245 kb on + strand at 3241.245 kb on + strand at 3241.245 kb on + strand at 3241.246 kb on - strand at 3241.246 kb on - strand at 3241.246 kb on - strand at 3241.409 kb on + strand at 3241.410 kb on - strand at 3241.535 kb on + strand, within baeS at 3241.536 kb on - strand, within baeS at 3241.813 kb on + strand, within baeS at 3241.814 kb on - strand, within baeS at 3241.826 kb on + strand, within baeS at 3242.023 kb on + strand, within baeS at 3242.023 kb on + strand, within baeS at 3242.023 kb on + strand, within baeS at 3242.024 kb on - strand, within baeS at 3242.140 kb on + strand, within baeS at 3242.141 kb on - strand, within baeS at 3242.256 kb on + strand, within baeS at 3242.256 kb on + strand, within baeS at 3242.257 kb on - strand, within baeS at 3242.257 kb on - strand, within baeS
Per-strain Table
Position Strand Gene LocusTag Fraction R2A_PIPES with Nickel (II) chloride 424 uM remove 3,239,628 + fepA OKGIIK_14880 0.72 -2.0 3,239,769 + fepA OKGIIK_14880 0.77 -2.2 3,239,770 - fepA OKGIIK_14880 0.77 -0.5 3,239,770 - fepA OKGIIK_14880 0.77 -0.8 3,239,770 - fepA OKGIIK_14880 0.77 -0.5 3,239,770 - fepA OKGIIK_14880 0.77 -0.5 3,239,770 - fepA OKGIIK_14880 0.77 -0.2 3,239,826 + fepA OKGIIK_14880 0.79 -0.3 3,239,826 + fepA OKGIIK_14880 0.79 -0.7 3,239,826 + fepA OKGIIK_14880 0.79 +1.1 3,239,826 + fepA OKGIIK_14880 0.79 +0.7 3,239,826 + fepA OKGIIK_14880 0.79 +1.8 3,239,826 + fepA OKGIIK_14880 0.79 -0.0 3,239,826 + fepA OKGIIK_14880 0.79 -1.3 3,239,827 - fepA OKGIIK_14880 0.79 -0.8 3,239,827 - fepA OKGIIK_14880 0.79 -1.2 3,239,827 - fepA OKGIIK_14880 0.79 -0.2 3,239,827 - fepA OKGIIK_14880 0.79 +1.2 3,239,827 - fepA OKGIIK_14880 0.79 -0.5 3,239,900 + fepA OKGIIK_14880 0.82 +1.0 3,239,900 + fepA OKGIIK_14880 0.82 -0.5 3,239,900 + fepA OKGIIK_14880 0.82 +0.5 3,239,901 - fepA OKGIIK_14880 0.82 -0.7 3,239,901 - fepA OKGIIK_14880 0.82 +1.0 3,239,901 - fepA OKGIIK_14880 0.82 +0.1 3,239,901 - fepA OKGIIK_14880 0.82 -0.1 3,239,931 + fepA OKGIIK_14880 0.83 -0.9 3,239,932 - fepA OKGIIK_14880 0.83 +1.0 3,240,015 + fepA OKGIIK_14880 0.86 -0.1 3,240,015 + fepA OKGIIK_14880 0.86 -0.2 3,240,015 + fepA OKGIIK_14880 0.86 -0.5 3,240,015 + fepA OKGIIK_14880 0.86 +0.2 3,240,016 - fepA OKGIIK_14880 0.86 +0.9 3,240,016 - fepA OKGIIK_14880 0.86 +1.1 3,240,016 - fepA OKGIIK_14880 0.86 -1.0 3,240,016 - fepA OKGIIK_14880 0.86 -0.7 3,240,016 - fepA OKGIIK_14880 0.86 +0.0 3,240,016 - fepA OKGIIK_14880 0.86 +0.7 3,240,057 + fepA OKGIIK_14880 0.88 -0.2 3,240,058 - fepA OKGIIK_14880 0.88 -0.4 3,240,058 - fepA OKGIIK_14880 0.88 -1.1 3,240,108 + fepA OKGIIK_14880 0.90 -2.6 3,240,109 - fepA OKGIIK_14880 0.90 -0.2 3,240,109 - fepA OKGIIK_14880 0.90 -0.8 3,240,109 - fepA OKGIIK_14880 0.90 -0.5 3,240,145 - -0.3 3,240,198 + +1.1 3,240,198 + -0.4 3,240,198 + -0.4 3,240,198 + -0.2 3,240,199 - -1.5 3,240,199 - +0.3 3,240,199 - +0.1 3,240,199 - -0.9 3,240,238 - +0.3 3,240,238 - +0.6 3,240,264 + +0.5 3,240,264 + -1.1 3,240,264 + +0.2 3,240,265 - +0.1 3,240,265 - -0.7 3,240,265 - -0.4 3,240,265 - -0.8 3,240,291 - +0.1 3,240,345 + -1.4 3,240,346 - +1.0 3,240,357 + -1.7 3,240,357 + -0.3 3,240,357 + -0.1 3,240,357 + -1.2 3,240,357 + +1.2 3,240,357 + +0.6 3,240,357 + -0.7 3,240,357 + -0.4 3,240,357 + -0.7 3,240,357 + -0.1 3,240,357 + +0.3 3,240,357 + -0.8 3,240,357 + -0.1 3,240,357 + +0.9 3,240,358 - -0.0 3,240,358 - -2.1 3,240,358 - +0.3 3,240,358 - -1.8 3,240,358 - +1.3 3,240,358 - -0.2 3,240,358 - +1.9 3,240,358 - -1.8 3,240,358 - +1.7 3,240,358 - +0.2 3,240,623 + -1.5 3,240,623 + +0.5 3,240,623 + +0.0 3,240,623 + +0.3 3,240,624 - +0.1 3,240,624 - -2.0 3,240,624 - +1.8 3,240,624 - -0.1 3,240,624 - -1.7 3,240,624 - -1.1 3,240,624 - +1.0 3,240,624 - +0.3 3,240,625 + -0.8 3,240,626 - -0.8 3,240,626 - -0.7 3,240,720 + ompR OKGIIK_14885 0.20 -2.2 3,240,720 + ompR OKGIIK_14885 0.20 -0.9 3,240,720 + ompR OKGIIK_14885 0.20 +0.8 3,240,720 + ompR OKGIIK_14885 0.20 -0.5 3,240,720 + ompR OKGIIK_14885 0.20 -1.1 3,240,721 - ompR OKGIIK_14885 0.20 -0.6 3,240,797 - ompR OKGIIK_14885 0.31 +0.8 3,240,872 + ompR OKGIIK_14885 0.42 -0.5 3,240,873 - ompR OKGIIK_14885 0.42 -0.6 3,240,873 - ompR OKGIIK_14885 0.42 +0.3 3,240,873 - ompR OKGIIK_14885 0.42 -2.1 3,240,873 - ompR OKGIIK_14885 0.42 -1.3 3,241,064 + ompR OKGIIK_14885 0.70 -0.3 3,241,065 - ompR OKGIIK_14885 0.70 -1.0 3,241,128 - ompR OKGIIK_14885 0.79 -2.2 3,241,128 - ompR OKGIIK_14885 0.79 -0.3 3,241,223 - -1.1 3,241,241 + -0.1 3,241,241 + -0.5 3,241,241 + +0.2 3,241,245 + -0.1 3,241,245 + +0.6 3,241,245 + +1.1 3,241,245 + -1.0 3,241,245 + +0.4 3,241,245 + +0.3 3,241,246 - -0.6 3,241,246 - -1.3 3,241,246 - -0.4 3,241,409 + -0.3 3,241,410 - +0.8 3,241,535 + baeS OKGIIK_14890 0.19 -1.1 3,241,536 - baeS OKGIIK_14890 0.19 -0.4 3,241,813 + baeS OKGIIK_14890 0.39 +0.9 3,241,814 - baeS OKGIIK_14890 0.39 +1.3 3,241,826 + baeS OKGIIK_14890 0.40 -0.7 3,242,023 + baeS OKGIIK_14890 0.55 +1.1 3,242,023 + baeS OKGIIK_14890 0.55 -1.0 3,242,023 + baeS OKGIIK_14890 0.55 -0.5 3,242,024 - baeS OKGIIK_14890 0.55 -0.9 3,242,140 + baeS OKGIIK_14890 0.63 +0.7 3,242,141 - baeS OKGIIK_14890 0.63 +0.6 3,242,256 + baeS OKGIIK_14890 0.71 -1.2 3,242,256 + baeS OKGIIK_14890 0.71 -0.2 3,242,257 - baeS OKGIIK_14890 0.72 -1.1 3,242,257 - baeS OKGIIK_14890 0.72 -1.1
Or see this region's nucleotide sequence