Experiment: R2A_PIPES with Nickel (II) chloride 424 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt hutI and OKGIIK_14825 are separated by 16 nucleotides OKGIIK_14825 and OKGIIK_14830 are separated by 60 nucleotides OKGIIK_14830 and OKGIIK_14835 are separated by 34 nucleotides
OKGIIK_14820: hutI - amidohydrolase family protein, at 3,227,797 to 3,229,032
hutI
OKGIIK_14825: OKGIIK_14825 - SDR family NAD(P)-dependent oxidoreductase, at 3,229,049 to 3,229,180
_14825
OKGIIK_14830: OKGIIK_14830 - 3-oxoacyl-[acyl-carrier-protein] reductase, at 3,229,241 to 3,229,813
_14830
OKGIIK_14835: OKGIIK_14835 - Lipocalin-like domain-containing protein, at 3,229,848 to 3,230,318
_14835
Position (kb)
3229
3230 Strain fitness (log2 ratio)
-2
-1
0
1 at 3228.079 kb on + strand, within hutI at 3228.079 kb on + strand, within hutI at 3228.079 kb on + strand, within hutI at 3228.079 kb on + strand, within hutI at 3228.080 kb on - strand, within hutI at 3228.080 kb on - strand, within hutI at 3228.080 kb on - strand, within hutI at 3228.251 kb on - strand, within hutI at 3228.251 kb on - strand, within hutI at 3228.373 kb on + strand, within hutI at 3228.373 kb on + strand, within hutI at 3228.374 kb on - strand, within hutI at 3228.542 kb on - strand, within hutI at 3228.816 kb on + strand, within hutI at 3228.887 kb on + strand, within hutI at 3228.887 kb on + strand, within hutI at 3228.888 kb on - strand, within hutI at 3228.990 kb on + strand at 3228.990 kb on + strand at 3228.994 kb on + strand at 3228.994 kb on + strand at 3228.994 kb on + strand at 3228.995 kb on - strand at 3228.995 kb on - strand at 3229.030 kb on + strand at 3229.030 kb on + strand at 3229.030 kb on + strand at 3229.030 kb on + strand at 3229.031 kb on - strand at 3229.068 kb on + strand, within OKGIIK_14825 at 3229.068 kb on + strand, within OKGIIK_14825 at 3229.068 kb on + strand, within OKGIIK_14825 at 3229.068 kb on + strand, within OKGIIK_14825 at 3229.069 kb on - strand, within OKGIIK_14825 at 3229.178 kb on + strand at 3229.178 kb on + strand at 3229.178 kb on + strand at 3229.179 kb on - strand at 3229.179 kb on - strand at 3229.179 kb on - strand at 3229.179 kb on - strand at 3229.179 kb on - strand at 3229.312 kb on + strand, within OKGIIK_14830 at 3229.312 kb on + strand, within OKGIIK_14830 at 3229.312 kb on + strand, within OKGIIK_14830 at 3229.313 kb on - strand, within OKGIIK_14830 at 3229.313 kb on - strand, within OKGIIK_14830 at 3229.313 kb on - strand, within OKGIIK_14830 at 3229.313 kb on - strand, within OKGIIK_14830 at 3229.490 kb on + strand, within OKGIIK_14830 at 3229.491 kb on - strand, within OKGIIK_14830 at 3229.491 kb on - strand, within OKGIIK_14830 at 3229.553 kb on + strand, within OKGIIK_14830 at 3229.553 kb on + strand, within OKGIIK_14830 at 3229.554 kb on - strand, within OKGIIK_14830 at 3229.554 kb on - strand, within OKGIIK_14830 at 3229.808 kb on + strand at 3229.808 kb on + strand at 3229.809 kb on - strand at 3229.899 kb on + strand, within OKGIIK_14835 at 3229.899 kb on + strand, within OKGIIK_14835 at 3229.899 kb on + strand, within OKGIIK_14835 at 3229.899 kb on + strand, within OKGIIK_14835 at 3229.900 kb on - strand, within OKGIIK_14835 at 3229.900 kb on - strand, within OKGIIK_14835 at 3229.900 kb on - strand, within OKGIIK_14835 at 3229.900 kb on - strand, within OKGIIK_14835 at 3229.900 kb on - strand, within OKGIIK_14835 at 3229.935 kb on + strand, within OKGIIK_14835 at 3229.936 kb on - strand, within OKGIIK_14835 at 3229.971 kb on + strand, within OKGIIK_14835 at 3229.971 kb on + strand, within OKGIIK_14835 at 3229.971 kb on + strand, within OKGIIK_14835 at 3229.971 kb on + strand, within OKGIIK_14835 at 3229.971 kb on + strand, within OKGIIK_14835 at 3229.972 kb on - strand, within OKGIIK_14835 at 3229.986 kb on + strand, within OKGIIK_14835 at 3229.986 kb on + strand, within OKGIIK_14835 at 3229.986 kb on + strand, within OKGIIK_14835 at 3229.987 kb on - strand, within OKGIIK_14835 at 3230.085 kb on + strand, within OKGIIK_14835 at 3230.085 kb on + strand, within OKGIIK_14835 at 3230.085 kb on + strand, within OKGIIK_14835 at 3230.085 kb on + strand, within OKGIIK_14835 at 3230.085 kb on + strand, within OKGIIK_14835 at 3230.085 kb on + strand, within OKGIIK_14835 at 3230.085 kb on + strand, within OKGIIK_14835 at 3230.086 kb on - strand, within OKGIIK_14835 at 3230.086 kb on - strand, within OKGIIK_14835 at 3230.086 kb on - strand, within OKGIIK_14835 at 3230.086 kb on - strand, within OKGIIK_14835 at 3230.086 kb on - strand, within OKGIIK_14835 at 3230.086 kb on - strand, within OKGIIK_14835 at 3230.086 kb on - strand, within OKGIIK_14835 at 3230.086 kb on - strand, within OKGIIK_14835 at 3230.086 kb on - strand, within OKGIIK_14835 at 3230.087 kb on + strand, within OKGIIK_14835 at 3230.094 kb on + strand, within OKGIIK_14835
Per-strain Table
Position Strand Gene LocusTag Fraction R2A_PIPES with Nickel (II) chloride 424 uM remove 3,228,079 + hutI OKGIIK_14820 0.23 +0.5 3,228,079 + hutI OKGIIK_14820 0.23 +0.3 3,228,079 + hutI OKGIIK_14820 0.23 +0.8 3,228,079 + hutI OKGIIK_14820 0.23 -1.6 3,228,080 - hutI OKGIIK_14820 0.23 -1.1 3,228,080 - hutI OKGIIK_14820 0.23 +0.8 3,228,080 - hutI OKGIIK_14820 0.23 -0.2 3,228,251 - hutI OKGIIK_14820 0.37 -1.7 3,228,251 - hutI OKGIIK_14820 0.37 -1.2 3,228,373 + hutI OKGIIK_14820 0.47 +0.2 3,228,373 + hutI OKGIIK_14820 0.47 -1.0 3,228,374 - hutI OKGIIK_14820 0.47 +0.3 3,228,542 - hutI OKGIIK_14820 0.60 +1.5 3,228,816 + hutI OKGIIK_14820 0.82 -0.5 3,228,887 + hutI OKGIIK_14820 0.88 -2.0 3,228,887 + hutI OKGIIK_14820 0.88 +0.5 3,228,888 - hutI OKGIIK_14820 0.88 -0.3 3,228,990 + +0.2 3,228,990 + -2.2 3,228,994 + -0.5 3,228,994 + -0.3 3,228,994 + -1.1 3,228,995 - -1.8 3,228,995 - -1.7 3,229,030 + -1.3 3,229,030 + -0.1 3,229,030 + -0.4 3,229,030 + +0.3 3,229,031 - +0.4 3,229,068 + OKGIIK_14825 0.14 -1.3 3,229,068 + OKGIIK_14825 0.14 -0.5 3,229,068 + OKGIIK_14825 0.14 -1.4 3,229,068 + OKGIIK_14825 0.14 -0.5 3,229,069 - OKGIIK_14825 0.15 -0.7 3,229,178 + +0.4 3,229,178 + -0.8 3,229,178 + +0.3 3,229,179 - -0.5 3,229,179 - -0.7 3,229,179 - -0.2 3,229,179 - +0.2 3,229,179 - -1.1 3,229,312 + OKGIIK_14830 0.12 -1.0 3,229,312 + OKGIIK_14830 0.12 -0.0 3,229,312 + OKGIIK_14830 0.12 +0.0 3,229,313 - OKGIIK_14830 0.13 +0.3 3,229,313 - OKGIIK_14830 0.13 -0.0 3,229,313 - OKGIIK_14830 0.13 +0.2 3,229,313 - OKGIIK_14830 0.13 +0.4 3,229,490 + OKGIIK_14830 0.43 +0.1 3,229,491 - OKGIIK_14830 0.44 -0.8 3,229,491 - OKGIIK_14830 0.44 +1.0 3,229,553 + OKGIIK_14830 0.54 -0.7 3,229,553 + OKGIIK_14830 0.54 +0.3 3,229,554 - OKGIIK_14830 0.55 -2.0 3,229,554 - OKGIIK_14830 0.55 +0.0 3,229,808 + -1.2 3,229,808 + -0.9 3,229,809 - +0.6 3,229,899 + OKGIIK_14835 0.11 -0.2 3,229,899 + OKGIIK_14835 0.11 -0.4 3,229,899 + OKGIIK_14835 0.11 -0.5 3,229,899 + OKGIIK_14835 0.11 +1.1 3,229,900 - OKGIIK_14835 0.11 -2.3 3,229,900 - OKGIIK_14835 0.11 +0.0 3,229,900 - OKGIIK_14835 0.11 -0.6 3,229,900 - OKGIIK_14835 0.11 -0.7 3,229,900 - OKGIIK_14835 0.11 -0.4 3,229,935 + OKGIIK_14835 0.18 -2.0 3,229,936 - OKGIIK_14835 0.19 -0.1 3,229,971 + OKGIIK_14835 0.26 +0.0 3,229,971 + OKGIIK_14835 0.26 +0.2 3,229,971 + OKGIIK_14835 0.26 -0.7 3,229,971 + OKGIIK_14835 0.26 +0.0 3,229,971 + OKGIIK_14835 0.26 -1.1 3,229,972 - OKGIIK_14835 0.26 -1.3 3,229,986 + OKGIIK_14835 0.29 +1.3 3,229,986 + OKGIIK_14835 0.29 -1.0 3,229,986 + OKGIIK_14835 0.29 -0.0 3,229,987 - OKGIIK_14835 0.30 -1.1 3,230,085 + OKGIIK_14835 0.50 -0.1 3,230,085 + OKGIIK_14835 0.50 -1.4 3,230,085 + OKGIIK_14835 0.50 -1.1 3,230,085 + OKGIIK_14835 0.50 -0.5 3,230,085 + OKGIIK_14835 0.50 +0.5 3,230,085 + OKGIIK_14835 0.50 +0.4 3,230,085 + OKGIIK_14835 0.50 -0.4 3,230,086 - OKGIIK_14835 0.51 -1.2 3,230,086 - OKGIIK_14835 0.51 -0.1 3,230,086 - OKGIIK_14835 0.51 +1.3 3,230,086 - OKGIIK_14835 0.51 -0.3 3,230,086 - OKGIIK_14835 0.51 +0.9 3,230,086 - OKGIIK_14835 0.51 -1.4 3,230,086 - OKGIIK_14835 0.51 -0.2 3,230,086 - OKGIIK_14835 0.51 -1.1 3,230,086 - OKGIIK_14835 0.51 +1.1 3,230,087 + OKGIIK_14835 0.51 +0.7 3,230,094 + OKGIIK_14835 0.52 -0.5
Or see this region's nucleotide sequence