Experiment: R2A_PIPES with Nickel (II) chloride 424 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt OKGIIK_14010 and yjbJ are separated by 241 nucleotides yjbJ and mnhG are separated by 48 nucleotides mnhG and mnhF overlap by 4 nucleotides mnhF and mnhE overlap by 4 nucleotides mnhE and phaD overlap by 4 nucleotides
OKGIIK_14010: OKGIIK_14010 - McrBC 5-methylcytosine restriction system component, at 3,045,621 to 3,046,925
_14010
OKGIIK_14015: yjbJ - CsbD family protein, at 3,047,167 to 3,047,364
yjbJ
OKGIIK_14020: mnhG - monovalent cation/H(+) antiporter subunit G, at 3,047,413 to 3,047,766
mnhG
OKGIIK_14025: mnhF - K+/H+ antiporter subunit F, at 3,047,763 to 3,048,044
mnhF
OKGIIK_14030: mnhE - Na+/H+ antiporter subunit E, at 3,048,041 to 3,048,529
mnhE
OKGIIK_14035: phaD - monovalent cation/H+ antiporter subunit D, at 3,048,526 to 3,050,124
phaD
Position (kb)
3047
3048
3049 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3046.807 kb on - strand at 3046.819 kb on + strand at 3046.819 kb on + strand at 3046.820 kb on - strand at 3046.820 kb on - strand at 3046.924 kb on - strand at 3046.924 kb on - strand at 3046.946 kb on + strand at 3046.947 kb on - strand at 3046.947 kb on - strand at 3046.947 kb on - strand at 3046.959 kb on + strand at 3046.959 kb on + strand at 3046.959 kb on + strand at 3046.960 kb on - strand at 3046.960 kb on - strand at 3047.016 kb on + strand at 3047.017 kb on - strand at 3047.143 kb on + strand at 3047.144 kb on - strand at 3047.271 kb on + strand, within yjbJ at 3047.279 kb on - strand, within yjbJ at 3047.279 kb on - strand, within yjbJ at 3047.279 kb on - strand, within yjbJ at 3047.279 kb on - strand, within yjbJ at 3047.279 kb on - strand, within yjbJ at 3047.363 kb on - strand at 3047.363 kb on - strand at 3047.469 kb on - strand, within mnhG at 3047.543 kb on + strand, within mnhG at 3047.544 kb on - strand, within mnhG at 3047.649 kb on - strand, within mnhG at 3047.649 kb on - strand, within mnhG at 3047.912 kb on - strand, within mnhF at 3047.912 kb on - strand, within mnhF at 3047.912 kb on - strand, within mnhF at 3048.156 kb on + strand, within mnhE at 3048.156 kb on + strand, within mnhE at 3048.566 kb on + strand at 3048.567 kb on - strand at 3048.971 kb on + strand, within phaD
Per-strain Table
Position Strand Gene LocusTag Fraction R2A_PIPES with Nickel (II) chloride 424 uM remove 3,046,807 - -1.7 3,046,819 + +0.4 3,046,819 + +2.0 3,046,820 - -0.8 3,046,820 - -0.1 3,046,924 - +0.7 3,046,924 - +0.1 3,046,946 + -0.1 3,046,947 - -0.9 3,046,947 - -0.3 3,046,947 - -2.0 3,046,959 + +1.0 3,046,959 + -1.9 3,046,959 + -0.5 3,046,960 - -0.5 3,046,960 - -0.8 3,047,016 + -2.2 3,047,017 - +0.6 3,047,143 + +0.1 3,047,144 - -0.3 3,047,271 + yjbJ OKGIIK_14015 0.53 +0.0 3,047,279 - yjbJ OKGIIK_14015 0.57 -0.9 3,047,279 - yjbJ OKGIIK_14015 0.57 -2.3 3,047,279 - yjbJ OKGIIK_14015 0.57 -0.2 3,047,279 - yjbJ OKGIIK_14015 0.57 +0.5 3,047,279 - yjbJ OKGIIK_14015 0.57 -0.9 3,047,363 - -1.3 3,047,363 - -0.3 3,047,469 - mnhG OKGIIK_14020 0.16 +0.0 3,047,543 + mnhG OKGIIK_14020 0.37 -0.1 3,047,544 - mnhG OKGIIK_14020 0.37 -0.3 3,047,649 - mnhG OKGIIK_14020 0.67 -3.4 3,047,649 - mnhG OKGIIK_14020 0.67 -1.7 3,047,912 - mnhF OKGIIK_14025 0.53 -1.3 3,047,912 - mnhF OKGIIK_14025 0.53 -2.6 3,047,912 - mnhF OKGIIK_14025 0.53 -0.4 3,048,156 + mnhE OKGIIK_14030 0.24 -0.5 3,048,156 + mnhE OKGIIK_14030 0.24 +0.4 3,048,566 + -1.0 3,048,567 - +0.3 3,048,971 + phaD OKGIIK_14035 0.28 -0.3
Or see this region's nucleotide sequence