Experiment: R2A_PIPES with Nickel (II) chloride 424 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt OKGIIK_13555 and thrA are separated by 356 nucleotides thrA and thrB overlap by 4 nucleotides
OKGIIK_13555: OKGIIK_13555 - Cytochrome C, at 2,937,027 to 2,937,782
_13555
OKGIIK_13560: thrA - homoserine dehydrogenase, at 2,938,139 to 2,939,335
thrA
OKGIIK_13565: thrB - homoserine kinase, at 2,939,332 to 2,940,324
thrB
Position (kb)
2938
2939
2940 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2937.196 kb on + strand, within OKGIIK_13555 at 2937.196 kb on + strand, within OKGIIK_13555 at 2937.196 kb on + strand, within OKGIIK_13555 at 2937.196 kb on + strand, within OKGIIK_13555 at 2937.196 kb on + strand, within OKGIIK_13555 at 2937.197 kb on - strand, within OKGIIK_13555 at 2937.197 kb on - strand, within OKGIIK_13555 at 2937.253 kb on + strand, within OKGIIK_13555 at 2937.253 kb on + strand, within OKGIIK_13555 at 2937.253 kb on + strand, within OKGIIK_13555 at 2937.254 kb on - strand, within OKGIIK_13555 at 2937.254 kb on - strand, within OKGIIK_13555 at 2937.254 kb on - strand, within OKGIIK_13555 at 2937.254 kb on - strand, within OKGIIK_13555 at 2937.259 kb on + strand, within OKGIIK_13555 at 2937.259 kb on + strand, within OKGIIK_13555 at 2937.259 kb on + strand, within OKGIIK_13555 at 2937.259 kb on + strand, within OKGIIK_13555 at 2937.448 kb on + strand, within OKGIIK_13555 at 2937.449 kb on - strand, within OKGIIK_13555 at 2937.449 kb on - strand, within OKGIIK_13555 at 2937.449 kb on - strand, within OKGIIK_13555 at 2937.469 kb on + strand, within OKGIIK_13555 at 2937.469 kb on + strand, within OKGIIK_13555 at 2937.469 kb on + strand, within OKGIIK_13555 at 2937.470 kb on - strand, within OKGIIK_13555 at 2937.493 kb on + strand, within OKGIIK_13555 at 2937.496 kb on + strand, within OKGIIK_13555 at 2937.496 kb on + strand, within OKGIIK_13555 at 2937.496 kb on + strand, within OKGIIK_13555 at 2937.496 kb on + strand, within OKGIIK_13555 at 2937.496 kb on + strand, within OKGIIK_13555 at 2937.496 kb on + strand, within OKGIIK_13555 at 2937.496 kb on + strand, within OKGIIK_13555 at 2937.496 kb on + strand, within OKGIIK_13555 at 2937.496 kb on + strand, within OKGIIK_13555 at 2937.496 kb on + strand, within OKGIIK_13555 at 2937.496 kb on + strand, within OKGIIK_13555 at 2937.496 kb on + strand, within OKGIIK_13555 at 2937.496 kb on + strand, within OKGIIK_13555 at 2937.497 kb on - strand, within OKGIIK_13555 at 2937.497 kb on - strand, within OKGIIK_13555 at 2937.497 kb on - strand, within OKGIIK_13555 at 2937.497 kb on - strand, within OKGIIK_13555 at 2937.497 kb on - strand, within OKGIIK_13555 at 2937.497 kb on - strand, within OKGIIK_13555 at 2937.497 kb on - strand, within OKGIIK_13555 at 2937.497 kb on - strand, within OKGIIK_13555 at 2937.497 kb on - strand, within OKGIIK_13555 at 2937.497 kb on - strand, within OKGIIK_13555 at 2937.497 kb on - strand, within OKGIIK_13555 at 2937.497 kb on - strand, within OKGIIK_13555 at 2937.497 kb on - strand, within OKGIIK_13555 at 2937.535 kb on + strand, within OKGIIK_13555 at 2937.535 kb on + strand, within OKGIIK_13555 at 2937.535 kb on + strand, within OKGIIK_13555 at 2937.535 kb on + strand, within OKGIIK_13555 at 2937.535 kb on + strand, within OKGIIK_13555 at 2937.536 kb on - strand, within OKGIIK_13555 at 2937.536 kb on - strand, within OKGIIK_13555 at 2937.536 kb on - strand, within OKGIIK_13555 at 2937.536 kb on - strand, within OKGIIK_13555 at 2937.556 kb on + strand, within OKGIIK_13555 at 2937.662 kb on + strand, within OKGIIK_13555 at 2937.663 kb on - strand, within OKGIIK_13555 at 2937.663 kb on - strand, within OKGIIK_13555 at 2937.793 kb on + strand at 2937.793 kb on + strand at 2937.793 kb on + strand at 2937.793 kb on + strand at 2937.793 kb on + strand at 2937.793 kb on + strand at 2937.794 kb on - strand at 2937.794 kb on - strand at 2937.933 kb on - strand at 2938.037 kb on + strand at 2938.037 kb on + strand at 2938.038 kb on - strand at 2938.038 kb on - strand at 2938.049 kb on + strand at 2938.049 kb on + strand at 2938.049 kb on + strand at 2938.050 kb on - strand at 2938.050 kb on - strand at 2938.050 kb on - strand at 2938.050 kb on - strand at 2938.058 kb on + strand at 2938.088 kb on + strand at 2938.088 kb on + strand at 2938.088 kb on + strand at 2938.088 kb on + strand at 2938.088 kb on + strand at 2938.088 kb on + strand at 2938.088 kb on + strand at 2938.088 kb on + strand at 2938.089 kb on - strand at 2938.089 kb on - strand at 2938.089 kb on - strand at 2938.089 kb on - strand at 2938.089 kb on - strand at 2938.089 kb on - strand at 2938.372 kb on + strand, within thrA at 2938.685 kb on + strand, within thrA at 2938.685 kb on + strand, within thrA at 2938.719 kb on + strand, within thrA at 2938.719 kb on + strand, within thrA at 2938.719 kb on + strand, within thrA at 2938.720 kb on - strand, within thrA at 2938.865 kb on + strand, within thrA at 2938.865 kb on + strand, within thrA at 2938.865 kb on + strand, within thrA at 2938.866 kb on - strand, within thrA at 2938.866 kb on - strand, within thrA at 2938.867 kb on + strand, within thrA at 2938.867 kb on + strand, within thrA at 2938.868 kb on - strand, within thrA at 2939.222 kb on + strand at 2939.223 kb on - strand at 2939.231 kb on - strand at 2939.753 kb on - strand, within thrB at 2940.182 kb on - strand, within thrB at 2940.335 kb on - strand at 2940.335 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A_PIPES with Nickel (II) chloride 424 uM remove 2,937,196 + OKGIIK_13555 0.22 -0.3 2,937,196 + OKGIIK_13555 0.22 +1.5 2,937,196 + OKGIIK_13555 0.22 +0.6 2,937,196 + OKGIIK_13555 0.22 -0.8 2,937,196 + OKGIIK_13555 0.22 -1.7 2,937,197 - OKGIIK_13555 0.22 -0.4 2,937,197 - OKGIIK_13555 0.22 -1.6 2,937,253 + OKGIIK_13555 0.30 -2.4 2,937,253 + OKGIIK_13555 0.30 -0.5 2,937,253 + OKGIIK_13555 0.30 -1.0 2,937,254 - OKGIIK_13555 0.30 +0.1 2,937,254 - OKGIIK_13555 0.30 -1.4 2,937,254 - OKGIIK_13555 0.30 -0.9 2,937,254 - OKGIIK_13555 0.30 -0.8 2,937,259 + OKGIIK_13555 0.31 +1.1 2,937,259 + OKGIIK_13555 0.31 +0.5 2,937,259 + OKGIIK_13555 0.31 +0.5 2,937,259 + OKGIIK_13555 0.31 +0.1 2,937,448 + OKGIIK_13555 0.56 +0.5 2,937,449 - OKGIIK_13555 0.56 -0.1 2,937,449 - OKGIIK_13555 0.56 +0.3 2,937,449 - OKGIIK_13555 0.56 -0.9 2,937,469 + OKGIIK_13555 0.58 -0.8 2,937,469 + OKGIIK_13555 0.58 -0.5 2,937,469 + OKGIIK_13555 0.58 -0.9 2,937,470 - OKGIIK_13555 0.59 +0.3 2,937,493 + OKGIIK_13555 0.62 +0.2 2,937,496 + OKGIIK_13555 0.62 +0.6 2,937,496 + OKGIIK_13555 0.62 -0.4 2,937,496 + OKGIIK_13555 0.62 -0.1 2,937,496 + OKGIIK_13555 0.62 +0.7 2,937,496 + OKGIIK_13555 0.62 -0.4 2,937,496 + OKGIIK_13555 0.62 -1.1 2,937,496 + OKGIIK_13555 0.62 -0.1 2,937,496 + OKGIIK_13555 0.62 +1.1 2,937,496 + OKGIIK_13555 0.62 -1.0 2,937,496 + OKGIIK_13555 0.62 -0.5 2,937,496 + OKGIIK_13555 0.62 +0.5 2,937,496 + OKGIIK_13555 0.62 -1.4 2,937,496 + OKGIIK_13555 0.62 -0.1 2,937,497 - OKGIIK_13555 0.62 -0.7 2,937,497 - OKGIIK_13555 0.62 +0.5 2,937,497 - OKGIIK_13555 0.62 +1.5 2,937,497 - OKGIIK_13555 0.62 -1.3 2,937,497 - OKGIIK_13555 0.62 +1.5 2,937,497 - OKGIIK_13555 0.62 -1.4 2,937,497 - OKGIIK_13555 0.62 -0.8 2,937,497 - OKGIIK_13555 0.62 -0.1 2,937,497 - OKGIIK_13555 0.62 +0.7 2,937,497 - OKGIIK_13555 0.62 +0.6 2,937,497 - OKGIIK_13555 0.62 -0.7 2,937,497 - OKGIIK_13555 0.62 +0.2 2,937,497 - OKGIIK_13555 0.62 -0.7 2,937,535 + OKGIIK_13555 0.67 -1.5 2,937,535 + OKGIIK_13555 0.67 +0.5 2,937,535 + OKGIIK_13555 0.67 +1.2 2,937,535 + OKGIIK_13555 0.67 +1.1 2,937,535 + OKGIIK_13555 0.67 +1.6 2,937,536 - OKGIIK_13555 0.67 -1.9 2,937,536 - OKGIIK_13555 0.67 -1.1 2,937,536 - OKGIIK_13555 0.67 +0.4 2,937,536 - OKGIIK_13555 0.67 -1.9 2,937,556 + OKGIIK_13555 0.70 +0.2 2,937,662 + OKGIIK_13555 0.84 -0.3 2,937,663 - OKGIIK_13555 0.84 -0.9 2,937,663 - OKGIIK_13555 0.84 +1.8 2,937,793 + -1.7 2,937,793 + -0.9 2,937,793 + -1.5 2,937,793 + -0.9 2,937,793 + +0.4 2,937,793 + -0.1 2,937,794 - -0.5 2,937,794 - -1.6 2,937,933 - -0.1 2,938,037 + +0.0 2,938,037 + -1.0 2,938,038 - -0.5 2,938,038 - +1.5 2,938,049 + -2.3 2,938,049 + -0.0 2,938,049 + -0.9 2,938,050 - +0.5 2,938,050 - -3.3 2,938,050 - -0.7 2,938,050 - -0.1 2,938,058 + -0.1 2,938,088 + +0.1 2,938,088 + +0.1 2,938,088 + -1.4 2,938,088 + -1.5 2,938,088 + +1.7 2,938,088 + -0.9 2,938,088 + -2.8 2,938,088 + -0.5 2,938,089 - -2.4 2,938,089 - +0.1 2,938,089 - -1.1 2,938,089 - -0.9 2,938,089 - -1.3 2,938,089 - -1.5 2,938,372 + thrA OKGIIK_13560 0.19 -1.5 2,938,685 + thrA OKGIIK_13560 0.46 +0.8 2,938,685 + thrA OKGIIK_13560 0.46 -1.3 2,938,719 + thrA OKGIIK_13560 0.48 -2.1 2,938,719 + thrA OKGIIK_13560 0.48 +0.0 2,938,719 + thrA OKGIIK_13560 0.48 -0.1 2,938,720 - thrA OKGIIK_13560 0.49 -1.7 2,938,865 + thrA OKGIIK_13560 0.61 -0.5 2,938,865 + thrA OKGIIK_13560 0.61 -1.1 2,938,865 + thrA OKGIIK_13560 0.61 -1.3 2,938,866 - thrA OKGIIK_13560 0.61 -0.1 2,938,866 - thrA OKGIIK_13560 0.61 -0.9 2,938,867 + thrA OKGIIK_13560 0.61 +0.2 2,938,867 + thrA OKGIIK_13560 0.61 -0.9 2,938,868 - thrA OKGIIK_13560 0.61 -1.7 2,939,222 + +0.1 2,939,223 - -1.1 2,939,231 - -1.3 2,939,753 - thrB OKGIIK_13565 0.42 -1.0 2,940,182 - thrB OKGIIK_13565 0.86 -2.0 2,940,335 - -0.1 2,940,335 - -1.5
Or see this region's nucleotide sequence