Experiment: R2A_PIPES with Nickel (II) chloride 424 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ubiA and ubiG are separated by 6 nucleotides ubiG and OKGIIK_13530 overlap by 23 nucleotides
OKGIIK_13520: ubiA - 4-hydroxybenzoate polyprenyltransferase, at 2,929,554 to 2,930,447
ubiA
OKGIIK_13525: ubiG - bifunctional 2-polyprenyl-6-hydroxyphenol methylase/3-demethylubiquinol 3-O-methyltransferase UbiG, at 2,930,454 to 2,931,164
ubiG
OKGIIK_13530: OKGIIK_13530 - 2-epi-5-epi-valiolone synthase, at 2,931,142 to 2,932,293
_13530
Position (kb)
2930
2931
2932 Strain fitness (log2 ratio)
-2
-1
0
1 at 2929.464 kb on + strand at 2930.053 kb on + strand, within ubiA at 2930.053 kb on + strand, within ubiA at 2930.053 kb on + strand, within ubiA at 2930.053 kb on + strand, within ubiA at 2930.053 kb on + strand, within ubiA at 2930.054 kb on - strand, within ubiA at 2930.054 kb on - strand, within ubiA at 2930.054 kb on - strand, within ubiA at 2930.054 kb on - strand, within ubiA at 2930.054 kb on - strand, within ubiA at 2930.054 kb on - strand, within ubiA at 2930.054 kb on - strand, within ubiA at 2930.117 kb on + strand, within ubiA at 2930.118 kb on - strand, within ubiA at 2930.198 kb on - strand, within ubiA at 2930.293 kb on + strand, within ubiA at 2930.293 kb on + strand, within ubiA at 2930.294 kb on - strand, within ubiA at 2930.294 kb on - strand, within ubiA at 2930.294 kb on - strand, within ubiA at 2930.303 kb on + strand, within ubiA at 2930.303 kb on + strand, within ubiA at 2930.304 kb on - strand, within ubiA at 2930.304 kb on - strand, within ubiA at 2930.304 kb on - strand, within ubiA at 2930.304 kb on - strand, within ubiA at 2930.305 kb on + strand, within ubiA at 2930.305 kb on + strand, within ubiA at 2930.305 kb on + strand, within ubiA at 2930.306 kb on - strand, within ubiA at 2930.482 kb on + strand at 2930.482 kb on + strand at 2930.482 kb on + strand at 2930.482 kb on + strand at 2930.482 kb on + strand at 2930.482 kb on + strand at 2930.482 kb on + strand at 2930.482 kb on + strand at 2930.483 kb on - strand at 2930.483 kb on - strand at 2930.483 kb on - strand at 2930.483 kb on - strand at 2930.483 kb on - strand at 2930.483 kb on - strand at 2930.483 kb on - strand at 2930.483 kb on - strand at 2930.483 kb on - strand at 2930.536 kb on + strand, within ubiG at 2930.536 kb on + strand, within ubiG at 2930.536 kb on + strand, within ubiG at 2930.536 kb on + strand, within ubiG at 2930.536 kb on + strand, within ubiG at 2930.537 kb on - strand, within ubiG at 2930.537 kb on - strand, within ubiG at 2930.537 kb on - strand, within ubiG at 2930.537 kb on - strand, within ubiG at 2930.537 kb on - strand, within ubiG at 2930.537 kb on - strand, within ubiG at 2930.537 kb on - strand, within ubiG at 2930.620 kb on + strand, within ubiG at 2930.621 kb on - strand, within ubiG at 2930.621 kb on - strand, within ubiG at 2930.621 kb on - strand, within ubiG at 2930.657 kb on - strand, within ubiG at 2930.657 kb on - strand, within ubiG at 2930.657 kb on - strand, within ubiG at 2930.657 kb on - strand, within ubiG at 2930.853 kb on + strand, within ubiG at 2930.853 kb on + strand, within ubiG at 2930.854 kb on - strand, within ubiG at 2930.854 kb on - strand, within ubiG at 2930.857 kb on + strand, within ubiG at 2930.863 kb on + strand, within ubiG at 2930.864 kb on - strand, within ubiG at 2931.507 kb on - strand, within OKGIIK_13530 at 2931.507 kb on - strand, within OKGIIK_13530 at 2931.863 kb on + strand, within OKGIIK_13530 at 2931.863 kb on + strand, within OKGIIK_13530 at 2931.911 kb on + strand, within OKGIIK_13530 at 2931.911 kb on + strand, within OKGIIK_13530 at 2932.100 kb on + strand, within OKGIIK_13530 at 2932.101 kb on - strand, within OKGIIK_13530
Per-strain Table
Position Strand Gene LocusTag Fraction R2A_PIPES with Nickel (II) chloride 424 uM remove 2,929,464 + -0.9 2,930,053 + ubiA OKGIIK_13520 0.56 -0.4 2,930,053 + ubiA OKGIIK_13520 0.56 -0.9 2,930,053 + ubiA OKGIIK_13520 0.56 +0.7 2,930,053 + ubiA OKGIIK_13520 0.56 +0.4 2,930,053 + ubiA OKGIIK_13520 0.56 -0.5 2,930,054 - ubiA OKGIIK_13520 0.56 +0.1 2,930,054 - ubiA OKGIIK_13520 0.56 -1.7 2,930,054 - ubiA OKGIIK_13520 0.56 -0.5 2,930,054 - ubiA OKGIIK_13520 0.56 -1.5 2,930,054 - ubiA OKGIIK_13520 0.56 +0.9 2,930,054 - ubiA OKGIIK_13520 0.56 -0.2 2,930,054 - ubiA OKGIIK_13520 0.56 +0.1 2,930,117 + ubiA OKGIIK_13520 0.63 -1.1 2,930,118 - ubiA OKGIIK_13520 0.63 -0.9 2,930,198 - ubiA OKGIIK_13520 0.72 -0.9 2,930,293 + ubiA OKGIIK_13520 0.83 -1.8 2,930,293 + ubiA OKGIIK_13520 0.83 -1.0 2,930,294 - ubiA OKGIIK_13520 0.83 -1.1 2,930,294 - ubiA OKGIIK_13520 0.83 -1.1 2,930,294 - ubiA OKGIIK_13520 0.83 +1.7 2,930,303 + ubiA OKGIIK_13520 0.84 -1.0 2,930,303 + ubiA OKGIIK_13520 0.84 +1.2 2,930,304 - ubiA OKGIIK_13520 0.84 -1.4 2,930,304 - ubiA OKGIIK_13520 0.84 +0.1 2,930,304 - ubiA OKGIIK_13520 0.84 -1.2 2,930,304 - ubiA OKGIIK_13520 0.84 -0.9 2,930,305 + ubiA OKGIIK_13520 0.84 +0.1 2,930,305 + ubiA OKGIIK_13520 0.84 -0.7 2,930,305 + ubiA OKGIIK_13520 0.84 -1.3 2,930,306 - ubiA OKGIIK_13520 0.84 +0.5 2,930,482 + +1.3 2,930,482 + -0.1 2,930,482 + -0.6 2,930,482 + -0.1 2,930,482 + -0.6 2,930,482 + -1.4 2,930,482 + -1.5 2,930,482 + -0.3 2,930,483 - -0.9 2,930,483 - -0.3 2,930,483 - +0.5 2,930,483 - +0.1 2,930,483 - -0.6 2,930,483 - +0.1 2,930,483 - -0.4 2,930,483 - +0.2 2,930,483 - -0.3 2,930,536 + ubiG OKGIIK_13525 0.12 -0.9 2,930,536 + ubiG OKGIIK_13525 0.12 -0.2 2,930,536 + ubiG OKGIIK_13525 0.12 +0.1 2,930,536 + ubiG OKGIIK_13525 0.12 -0.3 2,930,536 + ubiG OKGIIK_13525 0.12 -0.9 2,930,537 - ubiG OKGIIK_13525 0.12 -1.7 2,930,537 - ubiG OKGIIK_13525 0.12 -1.1 2,930,537 - ubiG OKGIIK_13525 0.12 -0.5 2,930,537 - ubiG OKGIIK_13525 0.12 +0.5 2,930,537 - ubiG OKGIIK_13525 0.12 -0.9 2,930,537 - ubiG OKGIIK_13525 0.12 -0.6 2,930,537 - ubiG OKGIIK_13525 0.12 -2.0 2,930,620 + ubiG OKGIIK_13525 0.23 -0.9 2,930,621 - ubiG OKGIIK_13525 0.23 -2.2 2,930,621 - ubiG OKGIIK_13525 0.23 -1.1 2,930,621 - ubiG OKGIIK_13525 0.23 -0.4 2,930,657 - ubiG OKGIIK_13525 0.29 -0.6 2,930,657 - ubiG OKGIIK_13525 0.29 -0.1 2,930,657 - ubiG OKGIIK_13525 0.29 +0.3 2,930,657 - ubiG OKGIIK_13525 0.29 +0.5 2,930,853 + ubiG OKGIIK_13525 0.56 +0.1 2,930,853 + ubiG OKGIIK_13525 0.56 -0.4 2,930,854 - ubiG OKGIIK_13525 0.56 -0.4 2,930,854 - ubiG OKGIIK_13525 0.56 -0.8 2,930,857 + ubiG OKGIIK_13525 0.57 -2.0 2,930,863 + ubiG OKGIIK_13525 0.58 -0.8 2,930,864 - ubiG OKGIIK_13525 0.58 -0.1 2,931,507 - OKGIIK_13530 0.32 -0.2 2,931,507 - OKGIIK_13530 0.32 +0.1 2,931,863 + OKGIIK_13530 0.63 -0.1 2,931,863 + OKGIIK_13530 0.63 -2.3 2,931,911 + OKGIIK_13530 0.67 +0.4 2,931,911 + OKGIIK_13530 0.67 +0.3 2,932,100 + OKGIIK_13530 0.83 +0.1 2,932,101 - OKGIIK_13530 0.83 +0.3
Or see this region's nucleotide sequence