Experiment: R2A_PIPES with Nickel (II) chloride 424 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ftsX and ftsE overlap by 4 nucleotides ftsE and OKGIIK_12950 are separated by 110 nucleotides OKGIIK_12950 and trxA are separated by 343 nucleotides trxA and rho are separated by 187 nucleotides
OKGIIK_12940: ftsX - permease-like cell division protein FtsX, at 2,792,318 to 2,793,298
ftsX
OKGIIK_12945: ftsE - cell division ATP-binding protein FtsE, at 2,793,295 to 2,793,972
ftsE
OKGIIK_12950: OKGIIK_12950 - ATP-dependent RNA helicase RhlB, at 2,794,083 to 2,795,861
_12950
OKGIIK_12955: trxA - thioredoxin TrxA, at 2,796,205 to 2,796,531
trxA
OKGIIK_12960: rho - transcription termination factor Rho, at 2,796,719 to 2,798,521
rho
Position (kb)
2794
2795
2796 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2793.093 kb on + strand, within ftsX at 2793.093 kb on + strand, within ftsX at 2793.093 kb on + strand, within ftsX at 2793.094 kb on - strand, within ftsX at 2793.094 kb on - strand, within ftsX at 2793.094 kb on - strand, within ftsX at 2793.094 kb on - strand, within ftsX at 2793.094 kb on - strand, within ftsX at 2793.584 kb on + strand, within ftsE at 2793.584 kb on + strand, within ftsE at 2793.585 kb on - strand, within ftsE at 2793.602 kb on + strand, within ftsE at 2794.017 kb on - strand at 2794.696 kb on + strand, within OKGIIK_12950 at 2794.696 kb on + strand, within OKGIIK_12950 at 2794.696 kb on + strand, within OKGIIK_12950 at 2794.696 kb on + strand, within OKGIIK_12950 at 2794.696 kb on + strand, within OKGIIK_12950 at 2794.696 kb on + strand, within OKGIIK_12950 at 2794.696 kb on + strand, within OKGIIK_12950 at 2794.696 kb on + strand, within OKGIIK_12950 at 2794.696 kb on + strand, within OKGIIK_12950 at 2794.696 kb on + strand, within OKGIIK_12950 at 2794.696 kb on + strand, within OKGIIK_12950 at 2794.696 kb on + strand, within OKGIIK_12950 at 2794.697 kb on - strand, within OKGIIK_12950 at 2794.697 kb on - strand, within OKGIIK_12950 at 2794.697 kb on - strand, within OKGIIK_12950 at 2794.697 kb on - strand, within OKGIIK_12950 at 2794.726 kb on + strand, within OKGIIK_12950 at 2794.726 kb on + strand, within OKGIIK_12950 at 2794.726 kb on + strand, within OKGIIK_12950 at 2794.727 kb on - strand, within OKGIIK_12950 at 2794.727 kb on - strand, within OKGIIK_12950 at 2794.727 kb on - strand, within OKGIIK_12950 at 2794.798 kb on + strand, within OKGIIK_12950 at 2794.798 kb on + strand, within OKGIIK_12950 at 2794.798 kb on + strand, within OKGIIK_12950 at 2794.798 kb on + strand, within OKGIIK_12950 at 2794.798 kb on + strand, within OKGIIK_12950 at 2794.798 kb on + strand, within OKGIIK_12950 at 2794.798 kb on + strand, within OKGIIK_12950 at 2794.798 kb on + strand, within OKGIIK_12950 at 2794.799 kb on - strand, within OKGIIK_12950 at 2794.799 kb on - strand, within OKGIIK_12950 at 2794.799 kb on - strand, within OKGIIK_12950 at 2794.799 kb on - strand, within OKGIIK_12950 at 2794.799 kb on - strand, within OKGIIK_12950 at 2794.799 kb on - strand, within OKGIIK_12950 at 2794.799 kb on - strand, within OKGIIK_12950 at 2794.825 kb on + strand, within OKGIIK_12950 at 2794.825 kb on + strand, within OKGIIK_12950 at 2794.825 kb on + strand, within OKGIIK_12950 at 2794.825 kb on + strand, within OKGIIK_12950 at 2794.825 kb on + strand, within OKGIIK_12950 at 2794.826 kb on - strand, within OKGIIK_12950 at 2794.826 kb on - strand, within OKGIIK_12950 at 2794.826 kb on - strand, within OKGIIK_12950 at 2794.826 kb on - strand, within OKGIIK_12950 at 2794.826 kb on - strand, within OKGIIK_12950 at 2795.110 kb on + strand, within OKGIIK_12950 at 2795.110 kb on + strand, within OKGIIK_12950 at 2795.110 kb on + strand, within OKGIIK_12950 at 2795.110 kb on + strand, within OKGIIK_12950 at 2795.110 kb on + strand, within OKGIIK_12950 at 2795.110 kb on + strand, within OKGIIK_12950 at 2795.110 kb on + strand, within OKGIIK_12950 at 2795.110 kb on + strand, within OKGIIK_12950 at 2795.111 kb on - strand, within OKGIIK_12950 at 2795.111 kb on - strand, within OKGIIK_12950 at 2795.111 kb on - strand, within OKGIIK_12950 at 2795.111 kb on - strand, within OKGIIK_12950 at 2795.188 kb on + strand, within OKGIIK_12950 at 2795.188 kb on + strand, within OKGIIK_12950 at 2795.188 kb on + strand, within OKGIIK_12950 at 2795.188 kb on + strand, within OKGIIK_12950 at 2795.188 kb on + strand, within OKGIIK_12950 at 2795.189 kb on - strand, within OKGIIK_12950 at 2795.189 kb on - strand, within OKGIIK_12950 at 2795.374 kb on + strand, within OKGIIK_12950 at 2795.374 kb on + strand, within OKGIIK_12950 at 2795.375 kb on - strand, within OKGIIK_12950 at 2795.375 kb on - strand, within OKGIIK_12950 at 2795.447 kb on - strand, within OKGIIK_12950 at 2795.461 kb on + strand, within OKGIIK_12950 at 2795.461 kb on + strand, within OKGIIK_12950 at 2795.461 kb on + strand, within OKGIIK_12950 at 2795.462 kb on - strand, within OKGIIK_12950 at 2795.802 kb on + strand at 2795.802 kb on + strand at 2795.802 kb on + strand at 2795.802 kb on + strand at 2795.803 kb on - strand at 2795.803 kb on - strand at 2795.816 kb on + strand at 2795.816 kb on + strand at 2795.816 kb on + strand at 2795.816 kb on + strand at 2795.816 kb on + strand at 2795.816 kb on + strand at 2795.816 kb on + strand at 2795.816 kb on + strand at 2795.816 kb on + strand at 2795.816 kb on + strand at 2795.816 kb on + strand at 2795.816 kb on + strand at 2795.816 kb on + strand at 2795.816 kb on + strand at 2795.816 kb on + strand at 2795.816 kb on + strand at 2795.816 kb on + strand at 2795.816 kb on + strand at 2795.816 kb on + strand at 2795.816 kb on + strand at 2795.816 kb on + strand at 2795.816 kb on + strand at 2795.816 kb on + strand at 2795.816 kb on + strand at 2795.817 kb on - strand at 2795.817 kb on - strand at 2795.817 kb on - strand at 2795.817 kb on - strand at 2795.817 kb on - strand at 2795.817 kb on - strand at 2795.817 kb on - strand at 2795.817 kb on - strand at 2795.817 kb on - strand at 2795.817 kb on - strand at 2795.817 kb on - strand at 2795.817 kb on - strand at 2795.817 kb on - strand at 2795.817 kb on - strand at 2795.817 kb on - strand at 2795.817 kb on - strand at 2795.817 kb on - strand at 2795.817 kb on - strand at 2795.817 kb on - strand at 2795.817 kb on - strand at 2795.817 kb on - strand at 2795.971 kb on - strand at 2795.971 kb on - strand at 2795.971 kb on - strand at 2795.971 kb on - strand at 2795.971 kb on - strand at 2796.102 kb on + strand at 2796.596 kb on + strand at 2796.618 kb on + strand at 2796.618 kb on + strand at 2796.618 kb on + strand at 2796.689 kb on + strand at 2796.689 kb on + strand at 2796.689 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A_PIPES with Nickel (II) chloride 424 uM remove 2,793,093 + ftsX OKGIIK_12940 0.79 +0.1 2,793,093 + ftsX OKGIIK_12940 0.79 -0.5 2,793,093 + ftsX OKGIIK_12940 0.79 -1.7 2,793,094 - ftsX OKGIIK_12940 0.79 -2.4 2,793,094 - ftsX OKGIIK_12940 0.79 +0.8 2,793,094 - ftsX OKGIIK_12940 0.79 +0.5 2,793,094 - ftsX OKGIIK_12940 0.79 -1.8 2,793,094 - ftsX OKGIIK_12940 0.79 -0.3 2,793,584 + ftsE OKGIIK_12945 0.43 +0.5 2,793,584 + ftsE OKGIIK_12945 0.43 +0.2 2,793,585 - ftsE OKGIIK_12945 0.43 -0.1 2,793,602 + ftsE OKGIIK_12945 0.45 +0.7 2,794,017 - +0.8 2,794,696 + OKGIIK_12950 0.34 -1.5 2,794,696 + OKGIIK_12950 0.34 +0.1 2,794,696 + OKGIIK_12950 0.34 -0.2 2,794,696 + OKGIIK_12950 0.34 -0.5 2,794,696 + OKGIIK_12950 0.34 +1.5 2,794,696 + OKGIIK_12950 0.34 +0.5 2,794,696 + OKGIIK_12950 0.34 -0.5 2,794,696 + OKGIIK_12950 0.34 -2.1 2,794,696 + OKGIIK_12950 0.34 -2.0 2,794,696 + OKGIIK_12950 0.34 +1.5 2,794,696 + OKGIIK_12950 0.34 -1.1 2,794,696 + OKGIIK_12950 0.34 -0.5 2,794,697 - OKGIIK_12950 0.35 +0.9 2,794,697 - OKGIIK_12950 0.35 +0.5 2,794,697 - OKGIIK_12950 0.35 -1.1 2,794,697 - OKGIIK_12950 0.35 -0.5 2,794,726 + OKGIIK_12950 0.36 -2.2 2,794,726 + OKGIIK_12950 0.36 -0.5 2,794,726 + OKGIIK_12950 0.36 +1.1 2,794,727 - OKGIIK_12950 0.36 -0.5 2,794,727 - OKGIIK_12950 0.36 -0.1 2,794,727 - OKGIIK_12950 0.36 -0.8 2,794,798 + OKGIIK_12950 0.40 -1.3 2,794,798 + OKGIIK_12950 0.40 -1.8 2,794,798 + OKGIIK_12950 0.40 -0.8 2,794,798 + OKGIIK_12950 0.40 -0.5 2,794,798 + OKGIIK_12950 0.40 -2.0 2,794,798 + OKGIIK_12950 0.40 -1.4 2,794,798 + OKGIIK_12950 0.40 -0.8 2,794,798 + OKGIIK_12950 0.40 +0.6 2,794,799 - OKGIIK_12950 0.40 -1.3 2,794,799 - OKGIIK_12950 0.40 -1.3 2,794,799 - OKGIIK_12950 0.40 -1.3 2,794,799 - OKGIIK_12950 0.40 +0.2 2,794,799 - OKGIIK_12950 0.40 +0.6 2,794,799 - OKGIIK_12950 0.40 -1.0 2,794,799 - OKGIIK_12950 0.40 -0.1 2,794,825 + OKGIIK_12950 0.42 -0.7 2,794,825 + OKGIIK_12950 0.42 -0.4 2,794,825 + OKGIIK_12950 0.42 -0.9 2,794,825 + OKGIIK_12950 0.42 +0.5 2,794,825 + OKGIIK_12950 0.42 +0.5 2,794,826 - OKGIIK_12950 0.42 +1.0 2,794,826 - OKGIIK_12950 0.42 -0.8 2,794,826 - OKGIIK_12950 0.42 -1.5 2,794,826 - OKGIIK_12950 0.42 -1.1 2,794,826 - OKGIIK_12950 0.42 +0.8 2,795,110 + OKGIIK_12950 0.58 -1.1 2,795,110 + OKGIIK_12950 0.58 -0.8 2,795,110 + OKGIIK_12950 0.58 +0.3 2,795,110 + OKGIIK_12950 0.58 -1.1 2,795,110 + OKGIIK_12950 0.58 -1.5 2,795,110 + OKGIIK_12950 0.58 +0.2 2,795,110 + OKGIIK_12950 0.58 -1.1 2,795,110 + OKGIIK_12950 0.58 -0.6 2,795,111 - OKGIIK_12950 0.58 +0.5 2,795,111 - OKGIIK_12950 0.58 +1.3 2,795,111 - OKGIIK_12950 0.58 -1.8 2,795,111 - OKGIIK_12950 0.58 +1.2 2,795,188 + OKGIIK_12950 0.62 -2.2 2,795,188 + OKGIIK_12950 0.62 -0.1 2,795,188 + OKGIIK_12950 0.62 +0.8 2,795,188 + OKGIIK_12950 0.62 +1.1 2,795,188 + OKGIIK_12950 0.62 -1.3 2,795,189 - OKGIIK_12950 0.62 -0.8 2,795,189 - OKGIIK_12950 0.62 +0.2 2,795,374 + OKGIIK_12950 0.73 +0.5 2,795,374 + OKGIIK_12950 0.73 +0.2 2,795,375 - OKGIIK_12950 0.73 -0.8 2,795,375 - OKGIIK_12950 0.73 -3.0 2,795,447 - OKGIIK_12950 0.77 -0.5 2,795,461 + OKGIIK_12950 0.77 -1.1 2,795,461 + OKGIIK_12950 0.77 -0.8 2,795,461 + OKGIIK_12950 0.77 -1.0 2,795,462 - OKGIIK_12950 0.78 +0.5 2,795,802 + -0.6 2,795,802 + -1.7 2,795,802 + +0.2 2,795,802 + -0.8 2,795,803 - +0.2 2,795,803 - -1.0 2,795,816 + +0.6 2,795,816 + -1.1 2,795,816 + +0.2 2,795,816 + -1.8 2,795,816 + -1.3 2,795,816 + -1.1 2,795,816 + +0.1 2,795,816 + +0.3 2,795,816 + +0.5 2,795,816 + +0.5 2,795,816 + -0.5 2,795,816 + -1.2 2,795,816 + -1.5 2,795,816 + -1.8 2,795,816 + -0.9 2,795,816 + -1.5 2,795,816 + -1.1 2,795,816 + -1.1 2,795,816 + -1.8 2,795,816 + -1.1 2,795,816 + -0.8 2,795,816 + +0.3 2,795,816 + -1.5 2,795,816 + +2.1 2,795,817 - -0.7 2,795,817 - +1.1 2,795,817 - -1.7 2,795,817 - +0.9 2,795,817 - -0.8 2,795,817 - -1.1 2,795,817 - +0.7 2,795,817 - -0.3 2,795,817 - +0.2 2,795,817 - -0.2 2,795,817 - +0.1 2,795,817 - +1.3 2,795,817 - +0.5 2,795,817 - -0.9 2,795,817 - +0.6 2,795,817 - -0.8 2,795,817 - -1.5 2,795,817 - +1.1 2,795,817 - -1.1 2,795,817 - +0.6 2,795,817 - +1.2 2,795,971 - -1.3 2,795,971 - -0.8 2,795,971 - +1.2 2,795,971 - -0.8 2,795,971 - -1.3 2,796,102 + -0.5 2,796,596 + +0.2 2,796,618 + +0.5 2,796,618 + +0.0 2,796,618 + -1.1 2,796,689 + -0.1 2,796,689 + -0.7 2,796,689 + +1.6
Or see this region's nucleotide sequence