Experiment: R2A_PIPES with Nickel (II) chloride 424 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt lpdA and aceF are separated by 15 nucleotides aceF and ccmA are separated by 228 nucleotides
OKGIIK_12590: lpdA - dihydrolipoyl dehydrogenase, at 2,710,870 to 2,712,654
lpdA
OKGIIK_12595: aceF - dihydrolipoyllysine-residue acetyltransferase, at 2,712,670 to 2,714,079
aceF
OKGIIK_12600: ccmA - multidrug ABC transporter ATP-binding protein, at 2,714,308 to 2,715,192
ccmA
Position (kb)
2712
2713
2714
2715 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 2711.709 kb on - strand, within lpdA at 2711.870 kb on + strand, within lpdA at 2712.233 kb on + strand, within lpdA at 2712.474 kb on + strand, within lpdA at 2712.475 kb on - strand, within lpdA at 2712.761 kb on + strand at 2712.884 kb on + strand, within aceF at 2712.884 kb on + strand, within aceF at 2712.885 kb on - strand, within aceF at 2712.885 kb on - strand, within aceF at 2712.885 kb on - strand, within aceF at 2713.083 kb on + strand, within aceF at 2713.085 kb on + strand, within aceF at 2713.467 kb on - strand, within aceF at 2713.780 kb on - strand, within aceF at 2713.780 kb on - strand, within aceF at 2714.127 kb on + strand at 2714.127 kb on + strand at 2714.127 kb on + strand at 2714.128 kb on - strand at 2714.128 kb on - strand at 2714.128 kb on - strand at 2714.128 kb on - strand at 2714.128 kb on - strand at 2714.128 kb on - strand at 2714.128 kb on - strand at 2714.144 kb on - strand at 2714.166 kb on - strand at 2714.166 kb on - strand at 2714.216 kb on + strand at 2714.216 kb on + strand at 2714.216 kb on + strand at 2714.217 kb on - strand at 2714.217 kb on - strand at 2714.217 kb on - strand at 2714.217 kb on - strand at 2714.217 kb on - strand at 2714.217 kb on - strand at 2714.217 kb on - strand at 2714.338 kb on + strand at 2714.339 kb on - strand at 2714.563 kb on + strand, within ccmA at 2714.707 kb on + strand, within ccmA at 2714.707 kb on + strand, within ccmA at 2714.707 kb on + strand, within ccmA at 2714.708 kb on - strand, within ccmA at 2714.708 kb on - strand, within ccmA at 2714.708 kb on - strand, within ccmA at 2714.708 kb on - strand, within ccmA at 2714.708 kb on - strand, within ccmA at 2714.877 kb on + strand, within ccmA at 2714.877 kb on + strand, within ccmA at 2714.877 kb on + strand, within ccmA at 2714.877 kb on + strand, within ccmA at 2714.877 kb on + strand, within ccmA at 2714.877 kb on + strand, within ccmA at 2714.878 kb on - strand, within ccmA at 2714.878 kb on - strand, within ccmA
Per-strain Table
Position Strand Gene LocusTag Fraction R2A_PIPES with Nickel (II) chloride 424 uM remove 2,711,709 - lpdA OKGIIK_12590 0.47 -0.8 2,711,870 + lpdA OKGIIK_12590 0.56 -0.3 2,712,233 + lpdA OKGIIK_12590 0.76 +0.3 2,712,474 + lpdA OKGIIK_12590 0.90 -1.1 2,712,475 - lpdA OKGIIK_12590 0.90 -0.1 2,712,761 + +0.2 2,712,884 + aceF OKGIIK_12595 0.15 -1.4 2,712,884 + aceF OKGIIK_12595 0.15 +0.2 2,712,885 - aceF OKGIIK_12595 0.15 -1.7 2,712,885 - aceF OKGIIK_12595 0.15 -1.3 2,712,885 - aceF OKGIIK_12595 0.15 -1.1 2,713,083 + aceF OKGIIK_12595 0.29 -0.4 2,713,085 + aceF OKGIIK_12595 0.29 -1.0 2,713,467 - aceF OKGIIK_12595 0.57 +0.2 2,713,780 - aceF OKGIIK_12595 0.79 -0.8 2,713,780 - aceF OKGIIK_12595 0.79 +0.8 2,714,127 + -1.7 2,714,127 + -0.5 2,714,127 + -1.7 2,714,128 - -0.5 2,714,128 - -1.8 2,714,128 - +1.0 2,714,128 - -1.3 2,714,128 - -0.3 2,714,128 - +0.3 2,714,128 - -1.0 2,714,144 - -0.2 2,714,166 - +0.2 2,714,166 - -0.5 2,714,216 + -1.1 2,714,216 + -0.3 2,714,216 + +0.6 2,714,217 - -0.2 2,714,217 - -0.1 2,714,217 - -0.2 2,714,217 - +0.5 2,714,217 - +0.5 2,714,217 - -0.1 2,714,217 - -1.3 2,714,338 + -1.3 2,714,339 - -0.6 2,714,563 + ccmA OKGIIK_12600 0.29 -0.8 2,714,707 + ccmA OKGIIK_12600 0.45 -0.1 2,714,707 + ccmA OKGIIK_12600 0.45 +0.2 2,714,707 + ccmA OKGIIK_12600 0.45 -1.4 2,714,708 - ccmA OKGIIK_12600 0.45 +1.5 2,714,708 - ccmA OKGIIK_12600 0.45 +0.4 2,714,708 - ccmA OKGIIK_12600 0.45 -0.4 2,714,708 - ccmA OKGIIK_12600 0.45 -2.8 2,714,708 - ccmA OKGIIK_12600 0.45 +1.0 2,714,877 + ccmA OKGIIK_12600 0.64 +0.5 2,714,877 + ccmA OKGIIK_12600 0.64 -0.5 2,714,877 + ccmA OKGIIK_12600 0.64 +0.2 2,714,877 + ccmA OKGIIK_12600 0.64 +0.2 2,714,877 + ccmA OKGIIK_12600 0.64 -1.0 2,714,877 + ccmA OKGIIK_12600 0.64 +0.8 2,714,878 - ccmA OKGIIK_12600 0.64 -2.5 2,714,878 - ccmA OKGIIK_12600 0.64 +0.1
Or see this region's nucleotide sequence