Experiment: R2A_PIPES with Nickel (II) chloride 424 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt glyA and ettA are separated by 190 nucleotides ettA and pyrF are separated by 103 nucleotides pyrF and OKGIIK_11455 are separated by 24 nucleotides
OKGIIK_11440: glyA - serine hydroxymethyltransferase, at 2,456,167 to 2,457,435
glyA
OKGIIK_11445: ettA - energy-dependent translational throttle protein EttA, at 2,457,626 to 2,459,293
ettA
OKGIIK_11450: pyrF - orotidine-5'-phosphate decarboxylase, at 2,459,397 to 2,460,215
pyrF
OKGIIK_11455: OKGIIK_11455 - hypothetical protein, at 2,460,240 to 2,460,497
_11455
Position (kb)
2457
2458
2459
2460 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 2456.849 kb on + strand, within glyA at 2456.849 kb on + strand, within glyA at 2456.850 kb on - strand, within glyA at 2456.850 kb on - strand, within glyA at 2456.850 kb on - strand, within glyA at 2456.850 kb on - strand, within glyA at 2456.906 kb on + strand, within glyA at 2456.907 kb on - strand, within glyA at 2456.972 kb on + strand, within glyA at 2456.973 kb on - strand, within glyA at 2457.002 kb on + strand, within glyA at 2457.002 kb on + strand, within glyA at 2457.002 kb on + strand, within glyA at 2457.003 kb on - strand, within glyA at 2457.003 kb on - strand, within glyA at 2457.119 kb on + strand, within glyA at 2457.119 kb on + strand, within glyA at 2457.120 kb on - strand, within glyA at 2457.120 kb on - strand, within glyA at 2457.132 kb on + strand, within glyA at 2457.167 kb on + strand, within glyA at 2457.167 kb on + strand, within glyA at 2457.228 kb on - strand, within glyA at 2457.228 kb on - strand, within glyA at 2457.228 kb on - strand, within glyA at 2457.228 kb on - strand, within glyA at 2457.228 kb on - strand, within glyA at 2457.242 kb on + strand, within glyA at 2457.243 kb on - strand, within glyA at 2457.243 kb on - strand, within glyA at 2457.245 kb on + strand, within glyA at 2457.246 kb on - strand, within glyA at 2457.246 kb on - strand, within glyA at 2457.246 kb on - strand, within glyA at 2457.246 kb on - strand, within glyA at 2457.272 kb on + strand, within glyA at 2457.272 kb on + strand, within glyA at 2457.273 kb on - strand, within glyA at 2457.273 kb on - strand, within glyA at 2457.273 kb on - strand, within glyA at 2457.273 kb on - strand, within glyA at 2457.273 kb on - strand, within glyA at 2457.273 kb on - strand, within glyA at 2457.273 kb on - strand, within glyA at 2457.323 kb on + strand at 2457.323 kb on + strand at 2457.323 kb on + strand at 2457.323 kb on + strand at 2457.323 kb on + strand at 2457.323 kb on + strand at 2457.323 kb on + strand at 2457.324 kb on - strand at 2457.324 kb on - strand at 2457.324 kb on - strand at 2457.324 kb on - strand at 2457.324 kb on - strand at 2457.324 kb on - strand at 2457.324 kb on - strand at 2457.324 kb on - strand at 2457.324 kb on - strand at 2457.405 kb on - strand at 2457.550 kb on + strand at 2457.550 kb on + strand at 2457.550 kb on + strand at 2457.550 kb on + strand at 2457.551 kb on - strand at 2457.551 kb on - strand at 2457.551 kb on - strand at 2457.552 kb on + strand at 2457.553 kb on - strand at 2457.590 kb on + strand at 2457.590 kb on + strand at 2457.590 kb on + strand at 2457.591 kb on - strand at 2457.591 kb on - strand at 2457.591 kb on - strand at 2457.638 kb on + strand at 2457.638 kb on + strand at 2457.639 kb on - strand at 2457.639 kb on - strand at 2457.644 kb on + strand at 2457.644 kb on + strand at 2457.644 kb on + strand at 2457.644 kb on + strand at 2457.644 kb on + strand at 2457.644 kb on + strand at 2457.644 kb on + strand at 2457.644 kb on + strand at 2457.644 kb on + strand at 2457.645 kb on - strand at 2457.645 kb on - strand at 2457.645 kb on - strand at 2457.846 kb on - strand, within ettA at 2457.885 kb on + strand, within ettA at 2457.886 kb on - strand, within ettA at 2457.950 kb on + strand, within ettA at 2457.959 kb on + strand, within ettA at 2458.155 kb on + strand, within ettA at 2458.155 kb on + strand, within ettA at 2458.156 kb on - strand, within ettA at 2458.286 kb on + strand, within ettA at 2458.358 kb on + strand, within ettA at 2458.358 kb on + strand, within ettA at 2458.358 kb on + strand, within ettA at 2458.358 kb on + strand, within ettA at 2458.358 kb on + strand, within ettA at 2458.358 kb on + strand, within ettA at 2458.358 kb on + strand, within ettA at 2458.359 kb on - strand, within ettA at 2458.359 kb on - strand, within ettA at 2458.533 kb on - strand, within ettA at 2458.796 kb on + strand, within ettA at 2458.796 kb on + strand, within ettA at 2458.796 kb on + strand, within ettA at 2458.797 kb on - strand, within ettA at 2458.797 kb on - strand, within ettA at 2458.907 kb on + strand, within ettA at 2458.907 kb on + strand, within ettA at 2458.907 kb on + strand, within ettA at 2458.907 kb on + strand, within ettA at 2458.907 kb on + strand, within ettA at 2458.908 kb on - strand, within ettA at 2458.908 kb on - strand, within ettA at 2459.207 kb on + strand at 2459.207 kb on + strand at 2459.208 kb on - strand at 2459.208 kb on - strand at 2459.208 kb on - strand at 2459.208 kb on - strand at 2459.208 kb on - strand at 2459.216 kb on + strand at 2459.216 kb on + strand at 2459.217 kb on - strand at 2459.217 kb on - strand at 2459.276 kb on + strand at 2459.276 kb on + strand at 2459.276 kb on + strand at 2459.277 kb on - strand at 2459.277 kb on - strand at 2459.277 kb on - strand at 2459.277 kb on - strand at 2459.292 kb on - strand at 2459.292 kb on - strand at 2459.292 kb on - strand at 2459.292 kb on - strand at 2459.307 kb on + strand at 2459.307 kb on + strand at 2459.307 kb on + strand at 2459.307 kb on + strand at 2459.307 kb on + strand at 2459.307 kb on + strand at 2459.308 kb on - strand at 2459.308 kb on - strand at 2459.308 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A_PIPES with Nickel (II) chloride 424 uM remove 2,456,849 + glyA OKGIIK_11440 0.54 -0.8 2,456,849 + glyA OKGIIK_11440 0.54 -1.0 2,456,850 - glyA OKGIIK_11440 0.54 -1.0 2,456,850 - glyA OKGIIK_11440 0.54 -0.9 2,456,850 - glyA OKGIIK_11440 0.54 -1.2 2,456,850 - glyA OKGIIK_11440 0.54 -1.6 2,456,906 + glyA OKGIIK_11440 0.58 -0.6 2,456,907 - glyA OKGIIK_11440 0.58 -1.4 2,456,972 + glyA OKGIIK_11440 0.63 -2.6 2,456,973 - glyA OKGIIK_11440 0.64 -0.6 2,457,002 + glyA OKGIIK_11440 0.66 -0.0 2,457,002 + glyA OKGIIK_11440 0.66 -1.4 2,457,002 + glyA OKGIIK_11440 0.66 +0.6 2,457,003 - glyA OKGIIK_11440 0.66 -0.8 2,457,003 - glyA OKGIIK_11440 0.66 -1.6 2,457,119 + glyA OKGIIK_11440 0.75 -0.4 2,457,119 + glyA OKGIIK_11440 0.75 -0.1 2,457,120 - glyA OKGIIK_11440 0.75 -0.8 2,457,120 - glyA OKGIIK_11440 0.75 -1.2 2,457,132 + glyA OKGIIK_11440 0.76 +0.1 2,457,167 + glyA OKGIIK_11440 0.79 +0.2 2,457,167 + glyA OKGIIK_11440 0.79 -1.0 2,457,228 - glyA OKGIIK_11440 0.84 -1.4 2,457,228 - glyA OKGIIK_11440 0.84 -0.4 2,457,228 - glyA OKGIIK_11440 0.84 -2.3 2,457,228 - glyA OKGIIK_11440 0.84 -1.0 2,457,228 - glyA OKGIIK_11440 0.84 -1.0 2,457,242 + glyA OKGIIK_11440 0.85 -2.1 2,457,243 - glyA OKGIIK_11440 0.85 +0.9 2,457,243 - glyA OKGIIK_11440 0.85 -1.0 2,457,245 + glyA OKGIIK_11440 0.85 -1.0 2,457,246 - glyA OKGIIK_11440 0.85 -1.2 2,457,246 - glyA OKGIIK_11440 0.85 -1.2 2,457,246 - glyA OKGIIK_11440 0.85 -2.8 2,457,246 - glyA OKGIIK_11440 0.85 -1.6 2,457,272 + glyA OKGIIK_11440 0.87 +0.2 2,457,272 + glyA OKGIIK_11440 0.87 -0.4 2,457,273 - glyA OKGIIK_11440 0.87 -3.0 2,457,273 - glyA OKGIIK_11440 0.87 -1.2 2,457,273 - glyA OKGIIK_11440 0.87 -1.4 2,457,273 - glyA OKGIIK_11440 0.87 -1.8 2,457,273 - glyA OKGIIK_11440 0.87 -1.0 2,457,273 - glyA OKGIIK_11440 0.87 -3.4 2,457,273 - glyA OKGIIK_11440 0.87 -2.3 2,457,323 + -1.4 2,457,323 + -2.1 2,457,323 + -0.8 2,457,323 + -1.4 2,457,323 + -1.2 2,457,323 + -0.4 2,457,323 + -1.1 2,457,324 - -0.4 2,457,324 - -1.7 2,457,324 - -1.3 2,457,324 - -2.1 2,457,324 - -0.8 2,457,324 - -1.2 2,457,324 - -1.4 2,457,324 - +0.6 2,457,324 - +0.8 2,457,405 - -1.1 2,457,550 + +0.6 2,457,550 + -0.6 2,457,550 + -0.0 2,457,550 + +1.7 2,457,551 - -0.6 2,457,551 - -0.3 2,457,551 - +0.6 2,457,552 + -0.5 2,457,553 - -1.0 2,457,590 + -0.4 2,457,590 + -0.6 2,457,590 + -0.4 2,457,591 - +0.3 2,457,591 - -1.0 2,457,591 - -0.9 2,457,638 + -1.4 2,457,638 + -0.7 2,457,639 - -1.6 2,457,639 - -3.0 2,457,644 + -1.6 2,457,644 + -1.0 2,457,644 + -0.8 2,457,644 + +1.4 2,457,644 + -1.0 2,457,644 + -1.7 2,457,644 + -1.5 2,457,644 + -1.2 2,457,644 + -1.3 2,457,645 - -2.1 2,457,645 - -1.6 2,457,645 - -2.0 2,457,846 - ettA OKGIIK_11445 0.13 -0.4 2,457,885 + ettA OKGIIK_11445 0.16 -0.7 2,457,886 - ettA OKGIIK_11445 0.16 -0.6 2,457,950 + ettA OKGIIK_11445 0.19 -0.6 2,457,959 + ettA OKGIIK_11445 0.20 -1.6 2,458,155 + ettA OKGIIK_11445 0.32 -2.1 2,458,155 + ettA OKGIIK_11445 0.32 -1.2 2,458,156 - ettA OKGIIK_11445 0.32 -2.2 2,458,286 + ettA OKGIIK_11445 0.40 -1.5 2,458,358 + ettA OKGIIK_11445 0.44 -1.0 2,458,358 + ettA OKGIIK_11445 0.44 -0.4 2,458,358 + ettA OKGIIK_11445 0.44 -1.5 2,458,358 + ettA OKGIIK_11445 0.44 -1.2 2,458,358 + ettA OKGIIK_11445 0.44 -2.4 2,458,358 + ettA OKGIIK_11445 0.44 -1.2 2,458,358 + ettA OKGIIK_11445 0.44 -0.8 2,458,359 - ettA OKGIIK_11445 0.44 -1.7 2,458,359 - ettA OKGIIK_11445 0.44 -0.9 2,458,533 - ettA OKGIIK_11445 0.54 -0.4 2,458,796 + ettA OKGIIK_11445 0.70 -0.6 2,458,796 + ettA OKGIIK_11445 0.70 -1.0 2,458,796 + ettA OKGIIK_11445 0.70 -1.2 2,458,797 - ettA OKGIIK_11445 0.70 -1.9 2,458,797 - ettA OKGIIK_11445 0.70 -1.2 2,458,907 + ettA OKGIIK_11445 0.77 -0.8 2,458,907 + ettA OKGIIK_11445 0.77 -1.6 2,458,907 + ettA OKGIIK_11445 0.77 -1.4 2,458,907 + ettA OKGIIK_11445 0.77 -1.4 2,458,907 + ettA OKGIIK_11445 0.77 +0.2 2,458,908 - ettA OKGIIK_11445 0.77 -1.0 2,458,908 - ettA OKGIIK_11445 0.77 -2.2 2,459,207 + -2.4 2,459,207 + -1.8 2,459,208 - -0.8 2,459,208 - -0.2 2,459,208 - -0.4 2,459,208 - -2.6 2,459,208 - -1.4 2,459,216 + +0.1 2,459,216 + -2.1 2,459,217 - -1.6 2,459,217 - -1.2 2,459,276 + -0.1 2,459,276 + +0.0 2,459,276 + -0.4 2,459,277 - +0.6 2,459,277 - +0.9 2,459,277 - -2.5 2,459,277 - +0.1 2,459,292 - -1.1 2,459,292 - -1.4 2,459,292 - -0.8 2,459,292 - -0.4 2,459,307 + +1.1 2,459,307 + -0.4 2,459,307 + -0.4 2,459,307 + +0.4 2,459,307 + +0.4 2,459,307 + +0.2 2,459,308 - -1.4 2,459,308 - -1.1 2,459,308 - +0.6
Or see this region's nucleotide sequence